欢迎访问作物学报,今天是

作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1771-1777.doi: 10.3724/SP.J.1006.2009.01771

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

根癌农杆菌介导转Bt基因苎麻的获得及其抗虫鉴定

符家平,汪波**,刘立军,杨金雨,王绪霞,邢秀龙,彭定祥*   

  1. 华中农业大学植物科技学院麻类研究室,湖北武汉430070
  • 收稿日期:2009-03-03 修回日期:2009-06-25 出版日期:2009-10-12 网络出版日期:2009-08-07
  • 通讯作者: 彭定祥,E-mail:pdxiang@mail.hzau.edu.cn; Tel:13397121298
  • 基金资助:

    本研究由公益性行业(农业)科研专项(nyhyzx07-018-16),国家自然科学基金项目(30800696),教育部博士点新教师基金项目(20070504059)资助。

Transgenic Ramie with Bt Gene  Mediated by Agrobacterium tumefacien and Evaluation of Its Pest-Resistance

FU Jia-Ping,WANG Bo**,LIU Li-Jun,YANG Jin-Yu,WANG Xu-Xia,XING Xiu-Long,PENG Ding-Xiang*   

  1. College of Plant Sciences and Technology,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2009-03-03 Revised:2009-06-25 Published:2009-10-12 Published online:2009-08-07
  • Contact: PENG Ding-Xiang,E-mail: pdxiang@mail.hzau.edu.cn; Tel: 13397121298

摘要:

采用本研究室建立的根癌农杆菌介导的高效遗传转化体系,转化苎麻优良品种芦竹青,获得了转Bt基因苎麻候选植株。通过PCRSouthern杂交等分子检测,证明Bt基因已经整合到部分候选植株基因组中。选取PCRSouthern杂交均为阳性的部分株系(T0)种植在大田,对这些植株进行室内抗虫鉴定并考察其主要农艺性状和品质性状,次年对T1代植株进行PCRSouthern杂交检测。结果表明, 与对照相比,T0代转Bt基因苎麻植株的抗虫性均强于对照,部分株系的抗虫性显著强于对照植株;且基本保持了亲本的优良性状;T1代植株中也含有Bt基因,表明Bt基因能稳定遗传,且T1代植株在大田的抗虫性明显强于非转基因植株。

关键词: 苎麻, 转基因, Bt基因, 赤蛱蝶, 抗虫

Abstract:

Agrobacterium tumefaciens-mediated approach is an effectively and widely used to introduce foreign DNA into plants. However, there were few reports published on ramie transformation mediated by Agrobacterium tumefaciens up to date. In the present study, transgenic ramie containing Bt gene were obtained by our highly efficient Agrobacterium-mediated transformation system using ramie Luzhuqing. Three T0 plants of transgenic ramie were chosen for further experiments and were transplanted into the fields. Laboratory assay of artificial infestation and the survey on agronomic traits and quality traits of T0 plants were conducted. The results showed that T0 transgenic plants exhibited more excellent pest resistance than the controls, and some were significantly superior to the controls. In addition, T0 transgenic plants maintained the main agronomic traits, such as plant height, stem diameter, rate of effective tiller, raw fiber weight, fiber production ratio from stem and fiber production ratio from phloem, and quality traits, such as strength, filament breaking tenacity, fiber fineness, fineness uniformity and glue content of raw fiber in Luzhuqing. PCR and Southern blotting analysis showed that T1 plants contained Bt gene, which indicated that Bt gene could be stably inherited. T1 plants in the fields performed highly pest resistance compared with non-transgenic plants.

Key words: Ramie, Transformation, Bt gene, Pyrameis indica(HerBst), Agrobaterium tumefaciens

[1] 单露英, 李俊, 李亮, 张丽, 王颢潜, 高佳琪, 吴刚, 武玉花, 张秀杰. 转基因玉米NK603基体标准物质研制[J]. 作物学报, 2022, 48(5): 1059-1070.
[2] 王渭霞, 赖凤香, 胡海燕, 何佳春, 魏琪, 万品俊, 傅强. 超低温11年保存期对转基因作物基体标准样品核酸检测的影响[J]. 作物学报, 2022, 48(1): 238-248.
[3] 李富, 王延周, 严理, 朱四元, 刘头明. 苎麻茎皮环状RNA表达谱分析[J]. 作物学报, 2021, 47(6): 1020-1030.
[4] 左香君, 房朋朋, 李加纳, 钱伟, 梅家琴. 有毛野生甘蓝(Brassica incana)抗蚜虫特性研究[J]. 作物学报, 2021, 47(6): 1109-1113.
[5] 李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明. 新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定[J]. 作物学报, 2021, 47(5): 789-798.
[6] 杨琴莉, 杨多凤, 丁林云, 赵汀, 张军, 梅欢, 黄楚珺, 高阳, 叶莉, 高梦涛, 严孙艺, 张天真, 胡艳. 棉花花器官突变体的鉴定及候选基因的克隆[J]. 作物学报, 2021, 47(10): 1854-1862.
[7] 付虹雨, 崔国贤, 李绪孟, 佘玮, 崔丹丹, 赵亮, 苏小惠, 王继龙, 曹晓兰, 刘婕仪, 刘皖慧, 王昕惠. 基于无人机遥感图像的苎麻产量估测研究[J]. 作物学报, 2020, 46(9): 1448-1455.
[8] 李俊,李亮,李夏莹,宋贵文,沈平,张丽,翟杉杉,柳方方,吴刚,张秀杰,武玉花. 转基因玉米MIR604基体标准物质研制[J]. 作物学报, 2020, 46(4): 473-483.
[9] 衡友强,游西龙,王艳. 费尔干猪毛菜病程相关蛋白SfPR1a基因的异源表达增强了烟草对干旱、盐及叶斑病的抗性[J]. 作物学报, 2020, 46(4): 503-512.
[10] 陈杉彬, 孙思凡, 聂楠, 杜冰, 何绍贞, 刘庆昌, 翟红. 甘薯IbCAF1基因的克隆及耐盐性、抗旱性鉴定[J]. 作物学报, 2020, 46(12): 1862-1869.
[11] 马硕, 焦悦, 杨江涛, 王旭静, 王志兴. 基因组测序技术解析耐除草剂转基因水稻G2-7的分子特征[J]. 作物学报, 2020, 46(11): 1703-1710.
[12] 郝志明,耿妙苗,温树敏,闫桂军,王睿辉,刘桂茹. 小麦抗麦红吸浆虫基因标记的开发与验证[J]. 作物学报, 2020, 46(02): 179-193.
[13] 张双双,王立伟,姚楠,郭光艳,夏玉凤,秘彩莉. 水稻OsUBA基因的表达及其在促进种子萌发和开花中的功能[J]. 作物学报, 2019, 45(9): 1327-1337.
[14] 周向阳,赵亮,狄佳春,陈旭升. 2个抗虫棉的外源Bt基因分子鉴定及其染色体定位[J]. 作物学报, 2019, 45(9): 1440-1445.
[15] 潘丽娟,陈娜,陈明娜,王通,王冕,陈静,杨珍,万勇善,禹山林,迟晓元,刘风珍. 花生AhPEPC1基因抑制表达的转基因后代转录组分析[J]. 作物学报, 2019, 45(7): 993-1001.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!