苎麻BnGCL1基因响应干旱胁迫的功能研究

doi:10.3724/SP.J.1006.2026.54037

摘要/Abstract

摘要： 干旱是影响植物生长发育的主要逆境之一。本研究探讨了苎麻(Boehmeria nivea L.) BnGCL1基因在干旱胁迫响应中的功能机制。结果表明，BnGCL1基因序列最大开放阅读框(ORF)为1581 bp，编码526个氨基酸，蛋白的等电点为5.79，分子量为59,123.98 Da，脂肪系数为78.78，稳定系数为37.42，属于稳定蛋白。BnGCL1基因在苎麻的根、茎、叶中均有表达，受干旱胁迫诱导表达。在干旱条件下，过表达BnGCL1植株的根长、鲜重、叶绿素a、叶绿素b均显著高于野生型，同时抗氧化酶如APX和γ-GCL活性以及渗透调节相关物质如GSSG、Pro等的代谢水平均发生显著变化。BnGCL1的过表达显著上调AtGST1、AtGST11、AtNCED3和AtWRKY40等干旱应答相关基因的表达水平，表明BnGCL1通过调控抗氧化系统和干旱响应信号通路参与植物的抗旱应答。通过VIGS技术验证了BnGCL1基因的沉默能够降低苎麻的耐旱性，表明其在苎麻干旱响应中的作用。本研究揭示了BnGCL1基因在干旱胁迫中的重要作用，为苎麻的抗旱分子机制研究和抗旱品种培育提供了理论依据。

关键词: 苎麻, BnGCL1, 干旱胁迫, 抗氧化酶, 分子机制

Abstract:

Drought is one of the major environmental stresses that affects plant growth and development. In this study, the functional role of the BnGCL1 gene in the drought stress response of ramie (Boehmeria nivea L.) was investigated. The results showed that the gene contains a maximum open reading frame (ORF) of 1581 bp, encoding a protein of 526 amino acids. The predicted protein has an isoelectric point of 5.79, a molecular weight of 59,123.98 Da, a fat index of 78.78, and an instability index of 37.42, indicating that it is a stable protein. BnGCL1 is expressed in the roots, stems, and leaves of ramie, and its expression is induced by drought stress. Under drought conditions, transgenic plants overexpressing BnGCL1 exhibited significantly greater root length, fresh weight, chlorophyll a, and chlorophyll b contents compared to wild-type plants. In addition, the activities of antioxidant enzymes such as APX and γ-GCL, as well as the levels of osmotic regulators including GSSG and Pro, were significantly altered. Overexpression of BnGCL1 also markedly upregulated the expression of drought-responsive genes, including AtGST1, AtGST11, AtNCED3, and AtWRKY40, suggesting that BnGCL1 enhances drought tolerance by modulating the antioxidant defense system and drought-responsive signaling pathways. Gene silencing experiments using VIGS technology further confirmed that suppression of BnGCL1 reduces drought tolerance in ramie, highlighting its critical role in drought response. This study reveals the important function of BnGCL1 under drought stress and provides a theoretical foundation for elucidating the molecular mechanisms of drought tolerance and for breeding drought-resistant ramie varieties.

Key words: Boehmeria nivea L., BnGCL1, drought stress, antioxidant enzymes, molecular mechanism

刘海波, 张蕾, 王立琦, 石晓丽, 周文莹, 崔国贤, 佘玮. 苎麻BnGCL1基因响应干旱胁迫的功能研究[J]. 作物学报, 0, (): 0-.

Liu Hai-Bo, Zhang Lei, Wang Li-Qi, Shi Xiao-Li, Zhou Wen-Ying, Cui Guo-Xian, She Wei. Functional study of the BnGCL1 gene in ramie (Boehmeria nivea L.) in response to drought stress[J]. Acta Agronomica Sinica, 0, (): 0-.

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