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作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1791-1797.doi: 10.3724/SP.J.1006.2009.01791

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

从野生二粒小麦导入普通小麦的抗白粉病基因MlWE18分子标记定位

韩俊1,2，张连松1，李根桥1，张宏涛1，解超杰1，杨作民1，孙其信1，刘志勇1,*   

  1. 1中国农业大学植物遗传育种系/农业生物技术重点实验室/农业部作物基因组学与遗传改良重点开放实验室/北京市作物遗传改良重点实验室/进一步作物杂种优势研究与利用重点实验室，北京100193；2北京农学院植物科技学院，北京102206
  • 收稿日期:2009-02-23 修回日期:2009-04-30 出版日期:2009-10-12 网络出版日期:2009-08-07
  • 通讯作者: 刘志勇，E-mail: zhiyongliu@cau.edu.cn
  • 基金资助:

    本研究由国家高技术研究发展计划（863计划）项目（2006AA100102，2006AA10Z1E9，2006AA10Z1C4，2006BAD01A02），国家自然科学基金项目（30425039，30771341），教育部长江学者和创新团队发展计划项目，高等学校学科创新引智计划项目（111-2-03）资助。

Molecular Mapping of Powdery Mildew Resistance Gene MlWE18 in Wheat Originated from Wild Emmer(Triticum turgidum var. dicoccoides)

HAN Jun1,2,ZHANG Lian-Song1,LI Gen-Qiao1,ZHANG Hong-Tao1,XIE Chao-Jie1,YANG Zuo-Min1,SUN Qi-Xin1,LIU Zhi-Yong1*   

  1. 1Department of Plant Genetics & Breeding,China Agricultural University/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Researdch & Utilization,Ministry of Education,Beijing 100193 China;2College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China
  • Received:2009-02-23 Revised:2009-04-30 Published:2009-10-12 Published online:2009-08-07
  • Contact: LIU Zhi-Yong，E-mail: zhiyongliu@cau.edu.cn

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摘要：

野生二粒小麦(Triticum turgidumvar. dicoccoides)是小麦抗白粉病遗传改良的重要基因资源。利用野生二粒小麦WE18与普通小麦品种(系)连续多次杂交和自交，育成对白粉病菌生理小种E09高度抵抗的小麦新品系3D249(京双27//燕大1817/WE18/3/温麦4，F7)。利用高感白粉病品系薛早和3D249组配杂交组合，获得杂种F1代、F2分离群体和F3代家系，进行苗期白粉病抗性鉴定和遗传分析。结果表明，小麦品系3D249对E09小种的抗性受显性单基因控制，暂命名该基因为MlWE18。利用集群分离分析法(BSA)和分子标记分析，发现4个简单重复序列(SSR)标记(Xwmc525、Xwmc273、Xcfa2040和Xcfa2240)、1个EST-STS标记(Xmag1759)和1个EST-STS序列标记(XE13-2)与抗白粉病基因MlWE18连锁，在遗传连锁图谱上的顺序为Xwmc525–Xcfa2040–Xwmc273–XE13-2–Xmag1759–MlWE18–Xcfa2240。SSR标记的染色体缺失系物理定位结果表明，抗白粉病基因MlWE18位于小麦7A染色体长臂末端的Bin 7AL 16–0.85–1.00。与已知定位于该染色体区域的Pm基因遗传连锁图谱比较表明，MlWE18与抗白粉病基因Pm1、MlIW72、PmU、Mlm2033和Mlm80均位于7AL相同染色体区段。

关键词: 普通小麦品系3D249, 野生二粒小麦, 抗白粉病基因, MlWE18, 分子标记

Abstract:

Wild emmer (Triticum turgidum var. dicoccoides) is an important germplasm for wheat improvement especially for resistance to powdery mildew caused by Blumeria graminis f. sp. tritici. Common wheat line 3D249 (Jingshuang 27//Yanda 1817/WE18/3/Wenmai 4, F7), a derivative of wild emmer accession WE18 and susceptible elite common wheat lines, was found highly resistant to prevailing powdery mildew isolate E09 at both seedling and adult plant stages in Beijing, China. Genetic analyses of the F1, F2 segregating population and their F3 progenies derived from a cross between susceptible line Xuezao and resistant line 3D249 indicated that the powdery mildew resistance of line 3D249 was controlled by a single dominant gene, temporarily designated MlWE18. By bulked segregant analysis (BSA), four SSR markers (Xwmc525, Xwmc273, Xcfa2040, and Xcfa2240), one RFLP-derived STS marker (Xmag1759) and one EST-STS marker (XE13-2) were found to be linked to MlWE18, with an order of Xwmc525–Xcfa2040–Xwmc273–XE13-2–Xmag1759–MlWE18–Xcfa2240 in the genetic linkage map. Using Chinese Spring nullisomic-tetrasomics, ditelosomics, and deletion lines, MlWE18 was physically mapped on chromosome 7AL terminal bin 7AL 16–0.85–1.00. However, the allelism of wild emmer derived MlWE18 to known powdery mildew resistance genes Pm1, PmU, MlIW72, Mlm2033, and Mlm80, all located on the same chromosome bin, need to be characterized in the future. The common wheat powdery mildew resistance line 3D249 provides useful new germplasm for disease resistance genes pyramiding and marker-assisted selection (MAS) in wheat breeding program.

Key words: Common wheat lin 3D249;Wild emmer(Triticum turgidum var.dicoccoides）, Powdery mildew Resistance genes, Mlwe18, Molecular markers

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