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作物学报 ›› 2010, Vol. 36 ›› Issue (08): 1286-1295.doi: 10.3724/SP.J.1006.2010.01286

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用SRAP标记构建甘薯分子连锁图谱

李爱贤1,2,刘庆昌1,*,王庆美2,张立明2,翟红1,刘树震3   

  1. 1 中国农业大学农业部作物基因组学与遗传改良重点开放实验室 / 北京市作物遗传改良重点实验室/教育部作物杂种优势研究与利用重点实验室，北京100193；2 山东省农业科学院作物研究所，山东济南250100；3 山东省济南市农业局，山东济南250021
  • 收稿日期:2010-01-28 修回日期:2010-04-20 出版日期:2010-08-12 网络出版日期:2010-05-20
  • 通讯作者: 刘庆昌, E-mail: liuqc@cau.edu.cn
  • 基金资助:
    本研究由国家高技术研究发展计划(863计划)项目(2006AA100107), 国家甘薯现代产业技术体系, 国家科技支撑计划项目(2006BAD01A06), 农业部引进国际先进农业科学技术计划(948计划)项目(2006-G21-02), 国家公益性行业(农业)科研专项(nyhyzx07-012-03)和山东省自然科学基金项目(Q2006D10)资助。

Establishment of Molecular Linkage Maps Using SRAP Markers in Sweet potato

LI Ai-Xian1,2,LIU Qing-chang1,*,WANG Qing-mei2,ZHANG Li-ming2,ZHAI Hong1,LIU Shu-Zhen3   

  1. 1 Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, Beijing 100193, China; 2 Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 3 Jinan Agricultural Bureau, Jinan 250021, China
  • Received:2010-01-28 Revised:2010-04-20 Published:2010-08-12 Published online:2010-05-20
  • Contact: LIU Qing-Chang,E-mail: liuqc@cau.edu.cn

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被引次数

48

摘要： 以高淀粉甘薯品种漯徐薯8号为母本，低淀粉甘薯品种郑薯20为父本，杂交得到的F1分离群体的240个单株，利用SRAP标记技术，共得到770个母本的多态性标记和523个父本的多态性标记，用JoinMap3.0软件和“双假测交”策略，分别构建了2个亲本的分子连锁图谱。其中漯徐薯8号的图谱包括由473个SRAP标记组成的81个连锁群，总图距为5 802.46 cM，标记间平均图距为10.16 cM；郑薯20的图谱包括由328个SRAP标记组成的66个连锁群，总图距为3 967.90 cM，标记间平均图距为12.02 cM。该高密度分子连锁图谱的构建，为甘薯分子标记辅助选择、基因定位及克隆研究奠定了基础。

关键词: 甘薯, 分子连锁图谱, SRAP标记

Abstract: Sweet potato [Ipomoea batatas (L.) Lam.] is an important vegetative reproduction crop. Despite its worldwide importance, molecular biology researches in sweet potato have lagged behind other crops due to its characteristics including polyploidy, outcrossing behavior and high heterozygosity. As a result, it is difficult to make a breakthrough for the improvement of sweet potato using conventional methods. In this study, sequence-related amplified polymorphism (SRAP) markers were applied to establish molecular genetic linkage maps with F1 population consisting of 240 individuals derived from a cross of sweet potato cv. Luoxushu 8 (high starch content cultivar) × cv. Zhengshu 20 (low starch content cultivar). A total of 770 and 523 SRAP markers were generated, respectively, in Luoxushu 8 and Zhengshu 20. Using the software of JoinMap 3.0 and the ‘double pseudo testcross strategy’, a molecular linkage map containing 81 linkage groups was established with 473 SRAP markers in Luoxushu 8, and the map covered 5 802.46 cM with an average marker interval of 10.16 cM. In Zhengshu 20，the molecular linkage map containing 66 linkage groups was constructed using 328 SRAP markers, and the map covered 3 967.90 cM with an average marker interval of 12.02 cM. The results will provide a powerful tool to facilitate the introgression of desired traits into cultivars, gene localization and map-based cloning techniques, and greatly increase breeding efficiency through marker-assisted selection (MAS) techniques.

Key words: Sweet potato, Molecular linkage map, SRAP marker

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