欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2010, Vol. 36 ›› Issue (08): 1286-1295.doi: 10.3724/SP.J.1006.2010.01286

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用SRAP标记构建甘薯分子连锁图谱

李爱贤1,2,刘庆昌1,*,王庆美2,张立明2,翟红1,刘树震3   

  1. 1 中国农业大学农业部作物基因组学与遗传改良重点开放实验室 / 北京市作物遗传改良重点实验室/教育部作物杂种优势研究与利用重点实验室，北京100193；2 山东省农业科学院作物研究所，山东济南250100；3 山东省济南市农业局，山东济南250021
  • 收稿日期:2010-01-28 修回日期:2010-04-20 出版日期:2010-08-12 网络出版日期:2010-05-20
  • 通讯作者: 刘庆昌, E-mail: liuqc@cau.edu.cn
  • 基金资助:
    本研究由国家高技术研究发展计划(863计划)项目(2006AA100107), 国家甘薯现代产业技术体系, 国家科技支撑计划项目(2006BAD01A06), 农业部引进国际先进农业科学技术计划(948计划)项目(2006-G21-02), 国家公益性行业(农业)科研专项(nyhyzx07-012-03)和山东省自然科学基金项目(Q2006D10)资助。

Establishment of Molecular Linkage Maps Using SRAP Markers in Sweet potato

LI Ai-Xian1,2,LIU Qing-chang1,*,WANG Qing-mei2,ZHANG Li-ming2,ZHAI Hong1,LIU Shu-Zhen3   

  1. 1 Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, Beijing 100193, China; 2 Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 3 Jinan Agricultural Bureau, Jinan 250021, China
  • Received:2010-01-28 Revised:2010-04-20 Published:2010-08-12 Published online:2010-05-20
  • Contact: LIU Qing-Chang,E-mail: liuqc@cau.edu.cn

PDF

2761

被引次数

48

摘要： 以高淀粉甘薯品种漯徐薯8号为母本，低淀粉甘薯品种郑薯20为父本，杂交得到的F1分离群体的240个单株，利用SRAP标记技术，共得到770个母本的多态性标记和523个父本的多态性标记，用JoinMap3.0软件和“双假测交”策略，分别构建了2个亲本的分子连锁图谱。其中漯徐薯8号的图谱包括由473个SRAP标记组成的81个连锁群，总图距为5 802.46 cM，标记间平均图距为10.16 cM；郑薯20的图谱包括由328个SRAP标记组成的66个连锁群，总图距为3 967.90 cM，标记间平均图距为12.02 cM。该高密度分子连锁图谱的构建，为甘薯分子标记辅助选择、基因定位及克隆研究奠定了基础。

关键词: 甘薯, 分子连锁图谱, SRAP标记

Abstract: Sweet potato [Ipomoea batatas (L.) Lam.] is an important vegetative reproduction crop. Despite its worldwide importance, molecular biology researches in sweet potato have lagged behind other crops due to its characteristics including polyploidy, outcrossing behavior and high heterozygosity. As a result, it is difficult to make a breakthrough for the improvement of sweet potato using conventional methods. In this study, sequence-related amplified polymorphism (SRAP) markers were applied to establish molecular genetic linkage maps with F1 population consisting of 240 individuals derived from a cross of sweet potato cv. Luoxushu 8 (high starch content cultivar) × cv. Zhengshu 20 (low starch content cultivar). A total of 770 and 523 SRAP markers were generated, respectively, in Luoxushu 8 and Zhengshu 20. Using the software of JoinMap 3.0 and the ‘double pseudo testcross strategy’, a molecular linkage map containing 81 linkage groups was established with 473 SRAP markers in Luoxushu 8, and the map covered 5 802.46 cM with an average marker interval of 10.16 cM. In Zhengshu 20，the molecular linkage map containing 66 linkage groups was constructed using 328 SRAP markers, and the map covered 3 967.90 cM with an average marker interval of 12.02 cM. The results will provide a powerful tool to facilitate the introgression of desired traits into cultivars, gene localization and map-based cloning techniques, and greatly increase breeding efficiency through marker-assisted selection (MAS) techniques.

Key words: Sweet potato, Molecular linkage map, SRAP marker

[1]Grattapaglia D, Sederoff R R. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics,1994, 137: 1121-1137
[2]Ukoskit K, Thompson P G, Watson J C E, Lawrence G W. Identifying a randomly amplified polymorphic DNA (RAPD) marker linked to a gene form root-knot nematode resistance in sweet potato.J Am Soc Hort Sci, 1997, 122: 818-821
[3]Thompson P G, Hong L L, Ukoskit K, Zhu Z. Genetic linkage of randomly amplified polymorphic DNA (RAPD) markers in sweet potato. J Am Soc Hort Sci, 1997, 122: 79-82
[4]Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica
[J].Theor Appl Genet,
[5]Kriegner A, Cervantes J C, Burg K, Mwanga R O M, Zhang D P. A genetic linkage map of sweetpotato
[Ipomoea batatas (L.) Lam.] based on AFLP markers. Mol Breed, 2003, 11: 169-185
[6]Cervantes-Flores J C, Craig Y G, Kriegner A, Kenneth P V, Faulk M A, Mwanga R O M, Sosinski B R. Development of a genetic linkage map and identification of homologous linkage groups in sweet potato using multiple-dose AFLP markers
[J].Mol Breed
[7]Wu J(吴洁), Tan W-F(谭文芳), He J-R(何俊蓉), Pu Z-G(蒲志刚), Wang D-Y(王大一), Zhang Z-S(张正圣), Zhan F-F(詹付凤), Yan W-Z(阎文昭). Construct on of SRAP linkage map and QTL mapping for starch content in sweet potato. Mol Plant Breed (分子植物育种), 2005, 3(6): 841-845 (in Chinese with English abstract)
[8]Jie Q(揭琴). Construction of a Genetic Lingkage Map and Development of AFLP Markers Linked to Stem Nematode Resistance Gene in Sweet potato
[Ipomoea batatas (L.) Lam.]. PhDDissertation of China Agricultural University, 2008 (in Chinese with English abstract)
[9]Bassam B J, Caetano-Anolles G, Gresshoff P M. Fast and sensitive silver-staining of DNA in polyacry-lamide gels
[J].Anal Biochem
[10]Jones A. Theoretical segregation ratios of qualitatively inherited characters for hexaploid sweet potato (Ipomoea batatas). USDA Tech Bull, 1967, p 1368
[11]Jin M-Y(金梦阳), Liu L-Z(刘列钊), Fu F-Y(付福友), Zhang Z-S(张正圣), Zhang X-K(张学昆), Li J-N(李加纳). Construction of a genetic linkage map in Brassica napus based on SRAP, SSR, AFLP and TRAP. Mol Plant Breed (分子植物育种), 2006, 4(4): 520-526 (in Chinese with English abstract)
[12]Ripol M I, Churchill G A, Da Silva J A, Sorrells M. Statistical aspects of genetic mapping in autopolyploids. Gene, 1999, 235: 31-41
[13]Lyttle T W. Segregation distorters
[J].Annu Rev Genet
[14]Xu S J, Singh R J, Hymowitz T. Establishment of a cytogenetic map soybean: progress and prospective. Soybean Genet Newslett, 1997, 24: 121-122
[15]Knox M R, Ellis T H N. Excess heterozygosity contributes to genetic map expansion in pea recombinant inbred populations. Genetics, 2002, 162: 861-873
[1] 王懿涵, 李富昌, 刘意, 朱国鹏. 甘薯IbOPR2基因启动子克隆及调控因子的筛选[J]. 作物学报, 2026, 52(4): 1268-1276.
[2] 蒋嘉卉, 江炳志, 刘冠明, 王章英, 唐朝臣. 紫肉甘薯品质性状的近红外光谱预测模型构建与优化[J]. 作物学报, 2026, 52(4): 1088-1102.
[3] 于永超, 刘明, 靳容, 赵鹏, 张强强, 王静, 朱晓亚, 唐忠厚. 甘薯高氮徒长的生理机制和转录组分析研究[J]. 作物学报, 2026, 52(3): 813-824.
[4] 杨璇, 李健康, 何婉洁, 李友军, 程相涵, 侯文邦. 硒肥对甘薯鲜切后褐变的影响及其机理解析[J]. 作物学报, 2026, 52(2): 578-589.
[5] 张海燕, 解备涛, 董顺旭, 张立明, 段文学. 滴灌条件下不同水溶肥种类和配比对鲜食甘薯产量和品质的影响[J]. 作物学报, 2025, 51(9): 2485-2500.
[6] 尹雨萌, 王雁楠, 康志河, 乔守晨, 卞倩倩, 李亚蔚, 曹郭郑, 赵国瑞, 徐丹丹, 杨育峰. 甘薯谷胱甘肽S-转移酶基因IbGSTU7的克隆及功能分析[J]. 作物学报, 2025, 51(7): 1736-1746.
[7] 阳新月, 肖人滈, 张林茜, 唐铭均, 孙光燕, 杜康, 吕长文, 唐道彬, 王季春. 不同生育期涝渍对甘薯抗逆生理特性及产量形成的影响[J]. 作物学报, 2025, 51(3): 744-754.
[8] 霍如雪, 葛祥菡, 石嘉, 李雪蕊, 戴圣杰, 刘振宁, 李宗芸. 甘薯组氨酸激酶蛋白IbHK5响应干旱和盐胁迫的功能分析[J]. 作物学报, 2025, 51(3): 650-666.
[9] 王语新, 陈天羽, 翟红, 张欢, 高少培, 何绍贞, 赵宁, 刘庆昌. 甘薯激酶基因IbHT1的克隆及抗旱性功能鉴定[J]. 作物学报, 2025, 51(2): 301-311.
[10] 磨冬枝, 刘芃君, 徐桂娣, 陈泽炀, 谭雪明, 黄英金, 程建峰, 曾研华. 有机无机肥配施对甘薯产量、品质及硒吸收的影响[J]. 作物学报, 2025, 51(12): 3342-3356.
[11] 张顺杰, 吴维泰, 冉禧玥, 赵梓含, 韩永辉, 吴正丹, 张凯. 甘薯β淀粉酶基因IbBAM48829的功能解析[J]. 作物学报, 2025, 51(11): 3096-3104.
[12] 孙一鸣, 田侠, 王少霞, 刘庆. 不同施磷水平对甘薯硒吸收、分配和转化的影响[J]. 作物学报, 2024, 50(6): 1608-1615.
[13] 杨春菊, 唐道彬, 张凯, 杜康, 黄红, 乔欢欢, 王季春, 吕长文. 氮钾减量配施对甘薯产量和品质的影响[J]. 作物学报, 2024, 50(5): 1341-1350.
[14] 朱晓亚, 张强强, 赵鹏, 刘明, 王静, 靳容, 于永超, 唐忠厚. 叶面喷施丹参碳点缓解甘薯低磷胁迫的转录组与代谢组学分析[J]. 作物学报, 2024, 50(2): 383-393.
[15] 蒋杨影, 唐铭均, 张林茜, 吕长文, 唐道彬, 王季春. 生长前期光照强度对甘薯叶片光合生理和结薯的影响[J]. 作物学报, 2024, 50(10): 2575-2585.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2