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作物学报 ›› 2010, Vol. 36 ›› Issue (08): 1296-1301.doi: 10.3724/SP.J.1006.2010.01296

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用RIL群体创造抗黄曲霉兼抗青枯病的高油花生新种质

廖伯寿,雷永,王圣玉,黄家权,任小平,姜慧芳,晏立英   

  1. 中国农业科学院油料作物研究所 / 农业部油料作物生物学重点开放实验室，湖北武汉 430062
  • 收稿日期:2010-01-26 修回日期:2010-04-18 出版日期:2010-08-12 网络出版日期:2010-06-11
  • 基金资助:

    本研究由国家自然科学基金项目(30270840,30571132),国家科技支撑计划(2006BAD13B05-2),国家科技基础条件平台项目(2005DKA21002-13),农业部作物种质资源保护项目(NB08-2130135-36)资助.

Novel High Oil Germplasm with Resistance to Aspergillus flavus and Bacterial Wilt Developed from Recombinant Inbred Lines

LIAO Bo-Shou,LEI Yong,WANG Sheng-Yu,HUANG Jia-Quan,LIN Xiao-Peng,JIANG Hui-Fang,YAN Li-Ying   

  1. Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences / Key Laboratory of Oil Crop Biology, Ministry of Agriculture, Wuhan 430062, China
  • Received:2010-01-26 Revised:2010-04-18 Published:2010-08-12 Published online:2010-06-11

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1271

被引次数

18

摘要： 协同提高抗青枯病花生品种的黄曲霉抗性及含油量是我国花生育种的重要目标之一。利用远杂9102×中花5号杂交后代衍生的重组近交系群体(RIL)，通过黄曲霉抗性、青枯病抗性鉴定及含油量测试，表明黄曲霉抗性受2对连锁并具累加作用主基因+加性多基因控制，含油量受2对具抑制作用主基因+加性多基因控制，RIL群体的黄曲霉抗性和含油量变异远远超过双亲的差异，表明它们均具有通过互补产生超亲性状的潜力，获得了抗黄曲霉或抗青枯病的高油后代家系18份，其中抗黄曲霉兼抗青枯病高油新种质1份(J091)。农艺性状和SSR分析结果表明，18份后代材料具丰富的遗传多样性，农艺性状优良，具有重要育种价值。

关键词: 种质创新, 抗黄曲霉, 抗青枯病, 高含油量, RIL群体

Abstract: One of the important goals for peanut breeding in China is to enhance the resistance to aflatoxin accumulation and bacterial wilt (Ralstonia solanacearum) as well as to increase the oil content in peanut. In the present study, recombinant inbred lines were constructed with the offspring derived from Yuanza 9120 ´ Zhonghua 5. Yuanza 9120 with average oil content has high resistance to bacterial wilt but no resistance to aflatoxin accumulation; Zhonghua 5 is high yielding, high susceptible to bacterial wilt and not resistant to aflatoxin accumulation. Resistances to aflatoxining and bacterial wilt were identified in F7-10 as well as the oil content and agronomic traits of lines were investigated. The results indicated that resistance to Aspergillus flavus was controlled accumulatively by two linked main genes and other additive genes, and oil content was controlled by two inhibitory main genes as well as other additive genes. There were significant difference for bacterial wilt resistance, resistance to Aspergillus flavus and oil content between the offspring and parents which presented heterobeltiosis. Eighteen high oil content lines with resistance to to Aspergillus flavus or bacterial wilt were obtained, one (J091) of which was resistant both to Aspergillus flavus and bacterial wilt. Analysis of agronomic traits and SSR data indicated that 18 lines were considerably diverse and may be used as potential breeding materials.

Key words: Peanut lines, Resistance to Aspergillus flavus, Bacterial wilt resistance, High oil content, Recombined inbreed lines

[1]Yu S-L(禹山林). Peanut Varieties and Their Pedigree in China (中国花生品种及其系谱). Shanghai: Shanghai Scientific and Technical Publishers, 2003 (in Chinese)
[2]Jiang H-F(姜慧芳), Wang S-Y(王圣玉), Ren X-P(任小平). Reaction of groundnut germplasm to Aspergillus flavus invasion. Chin Oil Crops Sci (中国油料作物学报), 2002, 24(1): 23-25 (in Chinese with English abstract)
[3]Jiang H-F(姜慧芳), Ren X-P(任小平), Wang S-Y(王圣玉). Evaluation for resistance to invasion and aflatoxin contamination caused by Aspergillus flavus in groundnut. Chin Oil Crops Sci (中国油料作物学报), 2005, 27(3): 21-25 (in Chinese withEnglish abstract)
[4]Jiang H-F(姜慧芳), Ren X-P(任小平), Wang S-Y(王圣玉), Liao B-S(廖伯寿). Durability of resistance to Aspergillus flavus infection and effect of intact testa without injury on aflatoxin production in peanut. Acta Agron Sin (作物学报), 2006, 32(6): 851-855 (in Chinese with English abstract)
[5]Mehan V K, McDonald D. Research on the aflatoxin problem in groundnut at ICRISAT
[J].Plant Soil
[6]Mehan V K, McDonald D, Rasagopalan K. Resistance of peanut genotypes to seed infection by Aspergillus flavus in field trials in India
[J].Peanut Sci
[7]Mehan V K, McDonald D, Haravu L J, Jayanthi S. The groundnut aflatoxin problem: review and literature database. Patancheru, India: ICRISAT Press, 1991
[8]Mixon A C. Peanut accessions resistance to seed infection by Aspergillus flavus
[J].Agron J
[9]Mixon A C. Reducing Aspergillus species infection of peanut seed using resistant genotypes. J Environ Qual, 1986, 15: 101-103
[10]Xiao D-R(肖达人), Wang S-Y(王圣玉), Qu Z(瞿桢). Progress on aflatoxin contamination of peanut. Peanut Sci Tech (花生科技), 1999, 28(suppl): 124-129 (in Chinese)
[11]Liang X-Q(梁炫强), Pan R-Z(潘瑞炽), Bin J-H(宾金华). Progress on mechanism of resistance to Aspergillus infection in peanut. Chin Oil Crops Sci (中国油料作物学报), 2000, 22(3): 77-80 (in Chinese with English abstract)
[12]Liang X-Q(梁炫强), Pan R-Z(潘瑞炽), Bin J-H(宾金华). Factors related to preharvest resistance to Aspergillus infection in peanut. Chin Oil Crops Sci (中国油料作物学报), 2000, 22(4): 67-70 (in Chinese with English abstract)
[13]Lei Y(雷永), Liao B-S(廖伯寿), Wang S-Y(王圣玉), Li D(李栋), Jiang H-F(姜慧芳). Identification of AFLP markers for resistance to seed infection by Aspergillus flavus in peanut (Arachis hypogaea L.). Acta Agron Sin (作物学报), 2005, 31(10): 1349-1353 (in Chinese with English abstract)
[14]Xue H Q, Isleib T G. Comparison of aflatoxin production in normal- and high-oleic backcross-derived peanut lines
[J].Plant Dis
[15]Xiao D-R(肖达人), Wang S-Y(王圣玉), Zhang H-L(张洪玲). Rapid identifying method for resistance to aflatoxin production in peanut. Chin Oil Crops Sci (中国油料作物学报), 1999, 21(3): 72-76 (in Chinese with English abstract)
[16]Jiang H-F(姜慧芳), duan N-X(段乃雄). Descriptors and Data Standard for Peanut (Arachis spp.). Beijing: China Agriculture Press, 2006. pp 11-36 (in Chinese)
[17]Zhang Y-M(章元明), Gai J-Y(盖钧镒). Identification of mixed major genes and polygenes inheritance model of quantitative traits by using DH or RIL population. Acta Genet Sin (遗传学报), 2000, 27(7): 634−640 (in Chinese with English abstract)
[18]Zhang Y-M(章元明), Gai J-Y(盖钧镒), Wang Y-J(王永军). An expansion of joint segregation analysis of quantitative trait for using P1, P2 and DH or RIL populations. Hereditas (遗传), 2001, 23(5): 467−470 (in Chinese with English abstract)
[19]Gai J-Y(盖钧镒), Zhang Y-M(章元明), Wang J-K(王建康). Genetic System of Quantitative Traits in Plants (植物数量遗传体系). Beijing: Science Press, 2003. pp 96−168 (in Chinese)
[20]Tang R-H(唐荣华), Zhuang W-J(庄伟建), Gao G-Q(高国庆), Han Z-Q(韩柱强), Zhong R-C(钟瑞春), He L-Q(贺梁琼), Zhou C-Q(周翠球). Simple sequence repeats polymorphism among accessions of var. vulgaris Harz in Arachis hypogaea L. Chin J Oil Crops Sci (中国油料作物学报), 2004, 26(2): 20−26 (in Chinese with English abstract)
[21]Liao B-S(廖伯寿), Duan N-X(段乃雄), Tan Y-J(谈宇俊), Tang G-Y(唐桂英), Li D(李栋), Jiang H-F(姜慧芳). Genetic studies on resistance to bacterial wilt in dragon groundnut: I. Genetic nature and combining abilities. Chin J Oil Crops Sci (中国油料), 1994, 16(4): 4−8 (in Chinese with English abstract)
[22]Liao B-S(廖伯寿), Lei Y(雷永), Wang S-Y(王圣玉), Li D(李栋), Huang J-Q(黄家权), Jiang H-F(姜慧芳), Ren X-P(任小平). Genetic diversity of peanut RILs and enhancement for high oil genotypes
[J].Acta Agron Sin (作物学报.2008, 34(6):999-
[23]Yu S-L(禹山林), Yang Q-L(杨庆利), Pan L-J(潘丽娟), Bo W-N(薄文娜). Genetic analysis for oil content of peanut seeds. J Plant Genet Resour (植物遗传资源学报), 2009, 10(3): 453−456 (in Chinese with English abstract)
[24]Chen S-L(陈四龙), Li Y-R(李玉荣), Cheng Z-S(程增书), Liao B-S(廖伯寿), Lei Y(雷永), Liu J-S(刘吉生). Heterosis and genetic analysis of oil content in peanut using mixed model of major gene and polygene. Sci Agric Sin (中国农业科学), 2009, 42(9): 3048−3057 (in Chinese with English abstract)
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