作物学报 ›› 2010, Vol. 36 ›› Issue (10): 1666-1673.doi: 10.3724/SP.J.1006.2010.01666
王希,李勇,朱延明*,柏锡,才华,纪巍
WANG Xi,LI Yong,ZHU Yan-Ming*,BAI Xi,CAI Hua,JI Wei
摘要: 野生大豆对非生物胁迫具有优良的抗性,是研究胁迫机制、挖掘抗性基因的理想材料。以耐盐野生大豆为试材,利用前期获得的盐胁迫基因芯片杂交结果和EST数据库,筛选出1个响应胁迫的3¢-EST,序列分析表明, 该EST编码膜联蛋白(annexin, ANN)。通过改进的5¢-RACE获得了该基因的完整编码区,翻译产物与拟南芥膜联蛋白的相似性为57%,将该基因命名为GsANN。GsANN基因的翻译产物具有膜联蛋白家族特征性的结构域,无强烈的亲水/疏水性,无信号肽,无跨膜结构域,与已报道的植物ANN蛋白一致。亚细胞定位结果表明GsANN蛋白在细胞内聚集于质膜附近。GsANN基因在野生大豆叶中受盐及干旱胁迫的诱导,超量表达GsANN基因的拟南芥对盐胁迫和干旱胁迫的敏感性提高,揭示了该基因与植物抗性间的关系。
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