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作物学报 ›› 2011, Vol. 37 ›› Issue (01): 119-126.doi: 10.3724/SP.J.1006.2011.00119

• 耕作栽培·生理生化 • 上一篇    下一篇

空间环境诱变小麦叶绿素缺失突变体的主要农艺性状和光合特性

赵洪兵,郭会君,赵林姝,古佳玉,赵世荣,李军辉,刘录祥*   

  1. 中国农业科学院作物科学研究所 / 国家农作物基因资源与基因改良重大科学工程 / 国家农作物航天诱变技术改良中心, 北京100081
  • 收稿日期:2010-04-23 修回日期:2010-06-23 出版日期:2011-01-12 网络出版日期:2010-11-16
  • 通讯作者: 刘录祥,E-mail:luxiang@263.net.cn,Tel:010-62122719
  • 基金资助:

    本研究由国家航天育种工程(发改高技[2003]138号), 国家高技术研究发展计划(863计划)项目(2007AA100102), 国家科技支撑计划项目(2008BAD97B01, 2009BAA24B05), 农业部农业公益性行业科研专项(200803034)和国际原子能机构项目(CRP14195, CPR5017)资助。

Agronomic Traits and Photosynthetic Characteristics of Chlorophyll-Deficient Wheat Mutant Induced by Spaceflight Environment

ZHAO Hong-Bing,GUO Hui-Jun,ZHAO Lin-Shu,GU Jia-Yu,ZHAO Shi-Rong,LI Jun-Hui,LIU Lu-Xiang*   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/ National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
  • Received:2010-04-23 Revised:2010-06-23 Published:2011-01-12 Published online:2010-11-16
  • Contact: LIU Lu-Xiang,E-mail:luxiang@263.net.cn,Tel:010-62122719

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被引次数

23

摘要： 叶绿素缺失突变体对于研究植物光合作用机制, 揭示叶绿素生物合成与降解途径, 发掘鉴定光合作用相关新基因以及了解基因间的相互作用有重要意义。空间诱变创制的小麦叶绿素缺失突变体Mt135的叶色表现为完全白化、条纹和绿3种类型, 其中完全白化株叶片完全白化, 于苗期死亡; 条纹株叶片为绿白相间的条纹, 能够正常成穗结实, 但其株高、穗长、株粒数、株粒重、千粒重都显著低于原始亲本, 生育期比原始亲本延长5~7 d; 绿株与原始亲本没有显著差异。初步遗传分析表明, Mt135是一个由核质基因共同作用的突变材料。对突变体及其原始亲本叶绿素荧光动力学参数分析表明, 当光照强度为110 μmol m-2 s-1时, 条纹株绿色组织光系统II的最大量子产量与原始亲本无显著差异, 光系统II的潜在活性显著低于原始亲本, 而光化学猝灭系数、非光化学猝灭系数、实际量子产量、调节性能量耗散的量子产量、非调节性能量耗散的量子产量在不同的生育期间变化不同。另外, 不同的光照强度下, 条纹株绿色组织的电子传递速率、光化学猝灭系数、实际量子产量的变化也不相同。条纹株白色组织和完全白化株则完全失去光合能力。上述结果证实, 小麦叶绿素缺失突变体Mt135的光合作用受到很大的影响, 光合特性发生了改变, 较高的光照强度在拔节期对突变体影响较大, 抽穗期影响相对较小。条纹株光合特性的改变与其株高、穗长和产量相关性状显著降低的结果相互印证。

关键词: 小麦, 空间诱变, 叶绿素缺失突变体, 农艺性状, 叶绿素荧光动力学参数

Abstract: Chlorophyll deficiency mutants are ideal materials to study photosynthetic mechanism, pathways of chlorophyll biosynthesis and degradation, and genes related to photosynthesis. The chlorophyll-deficient wheat (Triticum aestivum L.) mutant Mt135, induced by space mutagenesis, was investigated on main agronomic traits and photosynthesis characteristics with a comparison to its wild type. The leaf color of the Mt135 showed albino, stripe, and green phenotypes. The albino plants with entire albino leaves died at seedling stage, while plants with green-and-white striped leaves usually matured 5–7 d later than the wild type, and the plant height, spike length, grains and grain weight per plant, and 1000-grain weight were lower. The green plants were not significantly different from the wild type. Preliminary genetic analysis showed that the inheritance model of Mt135 was nucleo-cytoplasmic interaction. When exposed to photosynthetic active radiation (PAR) of 110 μmol m-2 s-1, the green tissue of striped plants (S-G) and the wild plant had no significant difference on the value of maximum photosystem II quantum yield, but the potential activity of photosystem II was significantly lower in the S-G plants than in the wild plant. The changes of the photochemical quenching, non-photochemical quenching, effective quantum yield, regulated non-photochemical energy dissipation, and non-regulated energy dissipation were different at various growth stages. In addition, the differences of the electron transport rate, photochemical quenching, and effective quantum yield between S-G plant and wild plant varied under different PAR conditions. The white tissue of striped plant and the albino plant completely lose the photosynthetic function. As a result, the photosynthesis of Mt135 was greatly restricted. High photosynthetic active radiation had tremendous impact on the mutant at elongation stage and relatively weak impact at heading stage. The changes of photosynthetic function of striped plant are in accordance with the reduction of their plant height, spike length, and yield related traits.

Key words: Triticum aestivum L., Space mutagenesis, Chlorophyll-deficient mutant, Agronomic traits, Chlorophyll fluorescence kinetic parameters

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