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作物学报 ›› 2013, Vol. 39 ›› Issue (03): 455-463.doi: 10.3724/SP.J.1006.2013.00455

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米出籽率、籽粒深度和百粒重的QTL分析

张伟强1,2,库丽霞2,张君2,韩赞平2,3,陈彦惠2,*   

  1. 1 驻马店市农业科学院, 河南驻马店463000; 2 河南农业大学农学院, 河南郑州450002; 3 河南科技大学农学院, 河南洛阳471003
  • 收稿日期:2012-11-16 修回日期:2012-11-16 出版日期:2013-03-12 网络出版日期:2013-01-04
  • 通讯作者: 陈彦惠, E-mail: chy989@sohu.com, Tel: 0371-63558032
  • 基金资助:

    本研究由河南省主要粮食作物遗传改良及优异种质创新基础研究和河南省玉米产业技术体系首席专家项目(S2010-02)资助。

QTL Analysis of Kernel Ratio, Kernel Depth and 100-Kernel Weight in Maize (Zea mays L.)

ZHANG Wei-Qiang1,2,KU Li-Xia2,ZHANG Jun2,HAN Zan-Ping2,3,CHEN Yan-Hui2,*   

  1. 1 Zhumadian Academy of Agricultural Sciences, Zhumadian 463000, China; 2 College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China ?; 3 College of Agronomy, Henan University of Science and Technology, Luoyang 471003, China
  • Received:2012-11-16 Revised:2012-11-16 Published:2013-03-12 Published online:2013-01-04
  • Contact: 陈彦惠, E-mail: chy989@sohu.com, Tel: 0371-63558032

摘要:

为研究玉米出籽率、籽粒深度、百粒重的遗传机制,以豫82×137组配的229F2:3家系为试验材料,采用复合区间作图法进行QTL定位分析。在3个环境下共检测到10QTL。其中,控制出籽率、籽粒深度、百粒重相关QTL分别为3个、3个和4个,它们的联合贡献率分别为35.5%28.1%39.0%位于第1染色体上介于标记umc1335umc2236之间控制出籽率的QTL qKR1b和位于第9染色体上介于标记bnlg1209–umc2095之间控制百粒重QTL q100-KW9b分别解释18.9%11.7%的表型变异,且作用方式为加性效应,分析表明这些区域可能包含调控玉米籽粒性状关键基因,对剖析玉米产量形成机制具有重要的参考价值。

关键词: 玉米, 出籽率, 籽粒深度, 百粒重, QTL分析

Abstract:

In order to study the genetic mechanism of kernel ratio (KR, %), kernel depth (KD), and 100-kernel weight (100-KW) in maize, we constructed a mapping population consisting of 229 F2:3 lines from the cross between inbred lines Yu 82 and Shen 137. QTL mapping and analysis for the three traits were conducted under the three environments by composite interval mapping (CIM) method. Three, three and four QTLs were detected for kernel ratio, kernel depth, and 100-kernel weight, with the joint contribution rate of 35.5%, 28.1%, and 39.0% respectively. One major QTL qKR1b controlling kernel ratio was detected on chromosome 1, locating in marker of interval umc1335–umc2236, explaining 18.9% of the phenotypic variation. Another major QTL q100-KW9b controlling 100-kernel weight was detected in marker of interval bnlg1209-umc2095 on chromosome 9, with explained 11.7% of the phenotypic variation, and gene action of additive effect. The results showed that some key genes for kernel characters are possibly contained in these regions, having an important researchvalue to analyze the genetic mechanism of maize yield formation.

Key words: Maize (Zea mays L.), Kernel ratio, Kernel depth, 100-kernel weight, QTL analysis

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