欢迎访问作物学报,今天是

作物学报 ›› 2013, Vol. 39 ›› Issue (07): 1148-1154.doi: 10.3724/SP.J.1006.2013.01148

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻类病变突变体c5的遗传分析与目标基因的精细定位

陈红霖1,2,**,向阳海1,**,赵纪莹1,2,尹德东1,2,梁国华3,翟文学1,江光怀1,*   

  1. 1 中国科学院遗传与发育生物学研究所, 北京 100101; 2 中国科学院大学, 北京 100049; 3 扬州大学农学院, 江苏扬州 225009
  • 收稿日期:2012-12-17 修回日期:2013-03-11 出版日期:2013-07-12 网络出版日期:2013-04-23
  • 通讯作者: 江光怀, E-mail: ghjiang@genetics.ac.cn, Tel: 010-64807622
  • 基金资助:

    本研究由国家转基因生物新品种培育重大专项(2011ZX08009-001, 003)和国家自然科学基金项目(31071379)资助。

Genetic Analysis and Gene Fine Mapping of Rice Lesion Mimic Mutant c5

CHEN Hong-Lin1,2,**,XIANG Yang-Hai1,**,ZHAO Ji-Ying1,2,YIN De-Dong1,2,LIANG Guo-Hua3,ZHAI Wen-Xue1,JIANG Guang-Huai1,*   

  1. 1 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China; 3 Agricultural College of Yangzhou University, Yangzhou 225009, China
  • Received:2012-12-17 Revised:2013-03-11 Published:2013-07-12 Published online:2013-04-23
  • Contact: 江光怀, E-mail: ghjiang@genetics.ac.cn, Tel: 010-64807622

摘要:

水稻类病变突变体c5是由粳稻品种中花11种子经化学诱变剂EMS (甲基磺酸乙酯)诱变处理得到的。该突变体叶片在三叶期开始出现近似圆形褐色斑点,经DAB染色和台酚蓝染色显示这些斑点积累了过多的H2O2并引起程序性细胞死亡。与野生型相比,突变体c5的成熟期株高从110.4 cm减少到74.6 cm,有效分蘖数和每穗着粒数分别减少23.7%28.5%,千粒重和结实率都显著降低,此外,c5还表现出对白叶枯病菌的广谱抗病性,对10个菲律宾生理小种都有强烈的抗性反应。遗传分析表明,c5的突变性状受单隐性核基因控制。利用c5和明恢86配组形成的包含6269个单株的F2群体和18个分子标记,将c基因限定在水稻第5染色体长臂STS标记S41S47之间大约102 kb的遗传距离内。序列分析发现该区间内其中有11个编码基因,且它们与现已报道的类病变基因都不同,暗示c5可能是一个新型类病变性状控制基因。

关键词: 水稻(Oryza sativa L.), 抗病, 类病变, 遗传分析, 基因定位

Abstract:

The lesion mimic mutant c5 was obtained bytreating the seeds of japonica variety Zhonghua 11 with chemical mutagen EMS (ethyl methane sulfonate). The phenotypes and main agronomic traits of the c5 mutant were investigated. There were many nearly circularbrown spots began to appear on c5 leaves at 3-leaf stage. These leaves were stained further with DAB and trypan blue respectively and indicated that there were excessive H2O2 production and clusters of dead cells in the lesion tissues. Compared with the wild-type, the height of c5 plant decreased from 110.4 cm to 74.6 cm, the number of effective tiller per plant and spikelet per panicle were reduced by 23.7% and 28.5% respectively, thousand-grain-weight and seed setting rate were significantly dropped.In addition, c5 is endowed a broad-spectrum resistance to bacterial blight resistance to ten Philippine races of X. oryzae pv. Oryzae (Xoo). Genetic analysis showed that the lesion mimic mutant c5 was controlled by a single recessive nuclear gene. Minghui86 was crossed with c5 and 6269 individuals of F2 population and 18 pairs of molecular markers were used for gene mapping. And thegene was further mapping to rice chromosome 5 in a 102 kb region between STS markers S41 and S47. Sequence analysis of the 102 kb region revealed that there were 11 candidates for c5 gene. All the 11 candidate genes were different from those lesion mimic genes reported previously, which suggested that the phenotype of c5 may be controlled by a novel gene.

Key words: Rice (Oryza sativa L.), Resistance, Lesion mimic, Genetic analysis, Gene mapping

[1]Dietrich R A, Richberg M H, Schmidt R, Dean C, Dangl J L. A novel zinc finger protein is encoded by the Arabidopsis LSD1 gene and functions as a negative regulator of plant cell death. Cell, 1997, 88: 685–694



[2]Buschges R, Hollricher K, Panstruga R, Simons G, Wolter M, Frijters A, Daelen R, Lee T, Diergaarde P, Groenendijk J, Topsch S, Vos P, Salamini F, Schulze-Lefert P. The barley mlo gene: A novel control element of plant pathogen resistance. Cell, 1997, 88: 695–705



[3]Gray J, Close P S, Briggs S P, Johal G S. A novel suppressor of cell death in plants encoded by the lls1 gene of maize. Cell, 1997, 89: 25–3l



[4]Badigannavar A M, Kale D M, Eapen S, Murty G S S. Inheritance of disease lesion mimic leaf trait in groundnut. J Hered, 2002, 93: 50–52



[5]Chen X-F(陈析丰), Jin Y(金杨), Ma B-J(马伯军). Progress on the studies of rice lesion mimics and their resistant mechanism to the pathogens. Acta Phytopathol Sin (植物病理学报), 2011, 41(1): 1–9 (in Chinese with English abstract)



[6]Matin M N, Saief S A, Rahman M M, Lee D H, Kang H, Lee D S, Kang S G. Comparative phenotypic and physiological characteristics of spotted leaf 6 (spl6) and brown leaf spot2 (bl2) lesion mimic mutants (LMM) in rice. Mol Cells, 30: 533–543



[7]Wu C, Bordeos A, Madamba M R, Baraoidan M, Ramos M, Wang G L, Leach J E, Leung H. Rice lesion mimic mutants with enhanced resistance to diseases. Mol Genet Genomics, 2008, 279: 605–619



[8]Yamanouchi U, Yano M, Lin H X, Ashikari M, Yamada K. A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein. Proc Natl Acad Sci USA, 2002, 99: 7530–7535



[9]Liu D-F(刘道峰), Cheng Z-K(程祝宽), Liu G-Q(刘国庆), Liu G-Z(刘国振), Wang B(王斌), Zhao X-F(赵显峰), Zhu L-H(朱立煌). Identification of rice lesion mimic mutant lmi and gene mapping. Chin Sci Bull (科学通报), 2003, 48(8): 831–835 (in Chinese)



[10]Babu R, Jiang C J, Xu X, Kottapalli K R, Takatsuji H, Miyao A. Isolation, fine mapping and expression profiling of a lesion mimic genotype, spl(NF4050-8) that confers blast resistance in rice. Theor Appl Genet, 122: 831–854



[11]Zeng L, Yin Z, Chen J, Leung H, Wang G L. Fine genetic mapping and physical delimitation of the lesion mimic gene Spl11 to a 160-kb DNA segment of the rice genome. Mol Genet Genomics, 2002, 268: 253–261



[12]Wu C, Bordeos A, Madamba M R, Baraoidan M, Ramos M, Wang G L, Leach J E, Leung H. Rice lesion mimic mutants with enhanced resistance to diseases. Mol Genet Genomics, 2008, 279: 605–619



[13]Liu G, Wang L, Zhou Z, Leung H, Wang G L, He C. Physical mapping of a rice lesion mimic gene, Spl1, to a 70-kb segment of rice chromosome 12. Mol Genet Genomics, 2004, 272: 108–115



[14]Sun C, Liu L, Tang J, Lin A, Zhang F, Fang J, Zhang G, Chu C. RLIN1, encoding a putative coproporphyrinogen III oxidase, is involved in lesion initiation in rice. J Genet Genomics, 2008, 38: 29–37



[15]Fujiwara T, Maisonneuve S, Isshiki M, Mizutani M, Chen L, Wong H L, Kawasaki T, Shimamoto K. Sekiguchi lesion gene encodes a cytochrome P450 monooxygenase that catalyzes conversion of tryptamine to serotonin in rice. J Biol Chem, 285: 11308–11313



[16]Zeng L R, Qu S H, Bordeos A, Yang C W, Baraoidan M, Yan H Y, Xie Q, Nahm B H, Leung H, Wang G L. Spotted leaf11, a negative regulator of plant cell death and defense, encodes a u-box/armadillo repeat protein endowed with E3 ubiquitin ligase activity. Plant Cell, 2004, 16: 2795–2808



[17]Mori M, Tomita C, Sugimoto K, Hasegawa M, Hayashi N, Dubouzet J G, Ochiai H, Sekimoto H, Hirohiko H, Kikuchi S. Isolation and molecular characterization of a Spotted leaf 18 mutant by modified activation-tagging in rice. Plant Mol Biol, 2007, 63: 847–860



[18]Qiao Y L, Jiang W Z, Lee J H, Park B S, Choi M S, Piao R H, Woo M O, Roh J H, Han L Z, Paek N C, Seo H S, Koh H J. SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit μl (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa). New Phytol, 2009, 184: 566–573



[19]Takahashi A, Agrawal G K Yamazaki M, Onosato K, Miyao A, Kawasaki T, Shimamoto K, Hirochika H. Rice Pti1a negatively regulates RAR1-dependent defense responses. Plant Cell, 2007, 19: 2940–2951



[20]Chern MFitzgerald H A, Canlas P E, Navarre D A, Ronald P C. Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light. Mol Plant Microbe Interact, 2005, 18: 511–520



[21]Wang L, Pei Z, Tian Y, He C. OsLSD1, a rice zinc finger protein, regulates programmed cell death and callus differentiation. Mol Plant Microbe Interact, 2005, 18: 375–384



[22]Kim J A, Agrawal G K, Rakwal R, Han K S, Kim K N, Yun C H, Heu S, Park S Y, Lee Y H. Jwa N S. Molecular cloning and mRNA expression analysis of a novel rice (Oryza sativa L.) MAPK kinase kinase, OsEDR1, an ortholog of Arabidopsis AtEDR1, reveal its role in defense/stress signalling pathways and development. Biochem Biophys Res Commun, 2003, 300: 868–876



[23]Peng D H, Qiu D W, Ruan L F, Zhou C F, Sun M. Protein elicitor PemG1 from Magnaporthe grisea induces systemic acquired resistance (SAR) in plants. Mol Plant Microbe Interact, 24: 1239–1246



[24]Hu G, N Yalpani, Briggs S P, Johal G S. A porphyrin pathway impairment is responsible for the phenotype of a dominant disease lesion mimic mutant of maize. Plant Cell, 1998, 10: 1095–1105



[25]Tang X Y, Xie M T, Kim Y J, Zhou J M, Klessig D F, Martin G B. Overexpression of Pto activates defense responses and confers broad resistance. Plant Cell, 1999, 11: 15–29



[26]McCouch S R, Kochert G, Yu Z H, Wang Z Y, Khush G S, Coffman W R, Tanksley S D. Molecular mapping of rice chromosome. Theor Appl Genet, 1988, 76: 815–829



[27]McCouch S R, Teytelman L, Xu Y B, Lobos K B, Clare K, Walton M, Fu B Y, Maghirang R, Li Z K, Xing Y Z, Zhang Q F, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res, 2002, 9: 257–279



[28]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Genetics Genomics, 1996, 252: 597–607



[29]Yin Z C, Chen J, Zeng L R, Goh M L, Leung H, Khush G S, Wang G L. Characterizing rice lesion mimic mutants and identifying a mutant with broad-spectrum resistance to rice and bacterial blight. Mol Plant-Microbe Interact, 2000, 13: 869–876



[30]Thordal-Christensen H, Zhang Z G, Wei Y D, Collinge D B. Subcellular localization of H2O2 in plants: H2O2 accumulation in papillae and hypersensitive response during the barley–powdery mildew interaction. Plant J, 1997, 11: 1187–1194



[31]Buege J A, Aust S D. Microsomal lipid peroxidation. Methods Enzymol, 1978, 52: 302–310



[32]Wang J-J(王建军), Zhu X-D(朱旭东), Wang L-Y(王林友), Zhang L-H(张利华), Xue Q-Z(薛庆中), He Z-H(何祖华). Physiological and genetic analysis of lesion resembling disease mutants (lrd) of Oryza sativa L. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 2004, 30(3): 331–338 (in Chinese with English abstract)



[33]Mizobuchi R, Hirabayashi H. Isolation and characterization of rice lesion mimic mutants with enhanced resistance to rice blast and bacterial blight. Plant Sci, 2002, 163: 345–353



[34]Jung Y H, Rakwal R, Agrawal G K, Shibato J, Kim J A, Lee M O, Choi P K, Jung S H, Kim S H, Koh H J, Yonekura M, Iwahashi H, Jwa N S. Differential expression of defense/stress-related marker proteins in leaves of a unique rice blast lesion mimic mutant (blm). J Proteome Res, 2006, 5: 2586–2598



[35]Li X-L(李秀兰), Wang P-R(王平荣), Qu Z-C(曲志才), Sun X-Q(孙小秋), Wang B(王兵), Deng X-J(邓晓建). Genetic analysis and fine mapping of a lesion mimic mutant C23 in rice. Sci Agric Sin (中国农业科学), 2010, 43(18): 3691–3697 (in Chinese with English abstract)

[1] 毛嘉琦, 黄朋雨, 赵佳佳, 郑兴卫, 武棒棒, 郝宇琼, 屈非, 刘成, 马朋涛, 郑军. 山西小麦品种白粉病抗性评价及抗病基因分子检测[J]. 作物学报, 2026, 52(6): 1669-1681.
[2] 王粤生, 葛冬冬, 程兰斐, 陈春环, 王长有, 刘新伦, 李停栋, 邓平川, 吉万全, 赵继新. 小麦-华山新麦草二体异代换系16DH25-7的分子细胞遗传学及抗病性鉴定[J]. 作物学报, 2026, 52(2): 433-445.
[3] 苏爱国, 肖森林, 易红梅, 段赛茹, 王帅帅, 张如养, 邢锦丰, 李春辉, 孙轩, 徐瑞斌, 徐田军, 李志勇, 张勇, 王荣焕, 宋伟, 赵久然. 玉米穗腐病的抗性遗传研究进展与育种应用[J]. 作物学报, 2026, 52(1): 1-13.
[4] 李世鹏, 陈才武, 张晶, 吕恬, 傅廷栋, 易斌. 基于改进U-Net++模型的油菜pol TCMS温敏两系育性等级鉴定及温度育性关系的量化研究[J]. 作物学报, 2025, 51(6): 1423-1434.
[5] 杨思杰, 杜启迪, 柴守玺, 熊宏春, 谢永盾, 赵林姝, 古佳玉, 郭会君, 刘录祥. 小麦小旗叶突变性状基因定位与遗传分析[J]. 作物学报, 2025, 51(6): 1548-1557.
[6] 袁鑫, 赵卓凡, 赵瑞清, 刘孝伟, 郑名敏, 刘育生, 董好胜, 邓丽娟, 曹墨菊, 黄强. 一份玉米小籽粒发育突变体mn-like1的遗传分析与分子鉴定[J]. 作物学报, 2025, 51(6): 1569-1581.
[7] 孟祥宇, 刁邓超, 刘雅睿, 李云丽, 孙玉晨, 吴玮, 赵雯, 汪妤, 吴建辉, 李春莲, 曾庆东, 韩德俊, 郑炜君. 小麦新品种西农877高产稳产的遗传特性解析[J]. 作物学报, 2025, 51(5): 1261-1276.
[8] 焦文娟, 白斌, 谢克莱·依拉木, 张飞飞, 贾秋珍, 耿洪伟, 程宇坤. 295份国内外小麦种质资源条锈病抗性评价及抗病基因分子检测[J]. 作物学报, 2025, 51(11): 2886-2898.
[9] 马骏, 陈锋, 殷贵鸿, 胡海燕, 魏学宁, 解超杰, 孔令让. 小麦抗茎基腐病遗传育种研究的现状与展望[J]. 作物学报, 2025, 51(10): 2559-2569.
[10] 孙美红, 王彩琴, 王蓉, 白文斌, 高振峰. Bacillus velezensis G-1·脂肽悬浮种衣剂的制备及其对高粱丝黑穗病菌的防侵染效果[J]. 作物学报, 2024, 50(6): 1467-1485.
[11] 苏帅, 刘孝伟, 牛群凯, 时子文, 侯雨微, 冯开洁, 荣廷昭, 曹墨菊. 玉米多叶矮化突变体lyd1的鉴定与基因克隆[J]. 作物学报, 2024, 50(5): 1124-1135.
[12] 余瑶, 王紫瑶, 周思睿, 刘鹏程, 叶亚峰, 马伯军, 刘斌美, 陈析丰. 水稻类病变突变体lms1的表型鉴定与抗病分子机制分析[J]. 作物学报, 2024, 50(4): 857-870.
[13] 王亚琪, 徐海风, 李曙光, 傅蒙蒙, 余希文, 赵志鑫, 杨加银, 赵团结. 大豆类病变皱叶突变体NT301遗传分析和2对基因定位[J]. 作物学报, 2024, 50(4): 808-819.
[14] 宋健, 熊亚俊, 陈伊洁, 徐瑞新, 刘康林, 郭庆元, 洪慧龙, 高华伟, 谷勇哲, 张丽娟, 郭勇, 阎哲, 刘章雄, 关荣霞, 李英慧, 王晓波, 郭兵福, 孙如建, 闫龙, 王好让, 姬月梅, 常汝镇, 王俊, 邱丽娟. 大豆巢式关联作图(NAM)群体构建及花色和种皮色遗传分析[J]. 作物学报, 2024, 50(3): 556-575.
[15] 胡瑶洁, 刘亚萍, 郑君妍, 韩婷, 马伯军, 叶亚峰, 刘斌美, 陈析丰. 水稻类病变早衰突变体lmes6的表型鉴定与基因定位[J]. 作物学报, 2024, 50(11): 2764-2774.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!