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作物学报 ›› 2013, Vol. 39 ›› Issue (09): 1576-1581.doi: 10.3724/SP.J.1006.2013.01576

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

抗全蚀病、根腐病的转PgPGIP1基因小麦的获得与鉴定

杨丽华1,2,王金凤2,杜丽璞2,徐惠君2,魏学宁2,李钊2,马翎健1,*,张增艳2,*   

  1. 1西北农林科技大学农学院，陕西杨凌 712100；2中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程 /农业部麦类生物学与遗传育种重点实验室，北京 100081
  • 收稿日期:2013-02-20 修回日期:2013-06-09 出版日期:2013-09-12 网络出版日期:2013-07-09
  • 通讯作者: 马翎健, E-mail: malingjian@nwsuaf.edu.cn; 张增艳, E-mail: zhangzengyan@caas.cn
  • 基金资助:

    本研究由国家转基因生物新品种培育科技重大专项(2011ZX08002-001和2013ZX08002-001)资助。

Generation and Characterization of PgPGIP1 Transgenic Wheat Plants with Enhanced Resistance to Take-All and Common Root Rot

YANG Li-Hua1,2,WANG Jin-Feng2,DU Li-Pu2,XU Hui-Jun2,WEI Xue-Ning2,LI Zhao2,MA Ling-Jian1,*,ZHANG Zeng-Yan2,*   

  1. 1 College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 National Key Facility of Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-02-20 Revised:2013-06-09 Published:2013-09-12 Published online:2013-07-09
  • Contact: 马翎健, E-mail: malingjian@nwsuaf.edu.cn; 张增艳, E-mail: zhangzengyan@caas.cn

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摘要：

全蚀病和根腐病是小麦(Triticum aestivum)重要的土传真菌病害。PgPGIP1是人参(Panax ginseng)的一种多聚半乳糖醛酸酶抑制蛋白，可以抑制部分病原真菌分泌的多聚半乳糖醛酸酶的活性。本研究人工合成了PgPGIP1基因，并构建PgPGIP1基因的单子叶植物表达载体pA25-PgPGIP1，通过基因枪介导法将其转入小麦品种扬麦18中。对转PgPGIP1基因的T0至T4代植株进行PCR、RT-PCR和Q-RT-PCR分析，并对其全蚀病和根腐病抗性进行鉴定。结果表明，PgPGIP1基因能够在4个转基因小麦株系中遗传、转录与表达。与未转基因的小麦扬麦18相比，4个转基因小麦株系对全蚀病与根腐病的抗性明显提高，说明PgPGIP1表达增强了转基因小麦对全蚀病与根腐病的抗性。

关键词: 人参多聚半乳糖醛酸酶抑制蛋白, 转基因小麦, 全蚀病, 根腐病, 抗性

Abstract:

Take-all disease is primarily caused by a soil-borne fungus Gaeumannomyces graminis var. tritici (Ggt). The disease common root rot is mainly caused by fungal pathogen Bipolaris sorokiniana (teleomorph Cochliobolus sativus). Both of them are important diseases of wheat (Triticum aestivum L.)worldwide. PgPGIP1, a polygalacturonase-inhibiting protein from Panax ginseng, can reduce the infection of some fungal phytopathogens through inhibiting the polygalacturonase activity of the pathogens. In this study, the full-length coding sequence of PgPGIP1 gene was synthesized, and the gene transformation vector pA25-PgPGIP1 was constructed. In the expression vector pA25-PgPGIP1, the PgPGIP1 gene can be expressed highly in monocot plants driving by maize ubiquitin promoter. Embryo callus of Yangmai 18 was bombarded by the gold particle containing pA25-PgPGIP1. The transgenic wheat plants from T0 to T4 generations were subjected to PCR, RT-PCR, and Q-RT-PCR assays. Results showed that the PgPGIP1 gene was indeed introduced into four transgenic wheat lines, and inherited from T0 to T4 generations and expressed. After inoculation with Ggt and B. sorokiniana, the disease evaluations  showed that the four transgenic wheat lines expressing PgPGIP1 displayed an enhanced resistances to take-all and common root rot compared with untransformed Yangmai 18.

Key words: Polygalacturonase-inhibiting protein (PGIP) in Panax ginseng, Transgenic wheat plants, Take-all, Common root rot, Resistance

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