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作物学报 ›› 2013, Vol. 39 ›› Issue (11): 2099-2105.doi: 10.3724/SP.J.1006.2013.02099

• 研究简报 • 上一篇    

菜用大豆籽粒不同部位蔗糖积累及关键酶活性研究

李彦生1,2,南海洋1,2,杜明3,连腾祥1,2,张秋英1,*,刘晓冰1   

  1. 1 中国科学院东北地理与农业生态研究所 / 黑土区农业生态重点实验室,黑龙江哈尔滨 150081;2 中国科学院大学,北京 100049;3 黑龙江省农垦科学院 / 水稻研究所,黑龙江佳木斯 154007
  • 收稿日期:2013-04-17 修回日期:2013-06-01 出版日期:2013-11-12 网络出版日期:2013-08-12
  • 通讯作者: 张秋英, E-mail: zhangqy@neigaehrb.ac.cn, Tel: 0451-86601320
  • 基金资助:

    本研究由哈尔滨市创新人才基金(2012RFXXN016)资助。

Sucrose Accumulation and Key Enzyme Activities in Different Parts of Seed in Vegetable Soybean

LI Yan-Sheng1,2,NAN Hai-Yang1,2,DU Ming3,LIAN Teng-Xiang1,2,ZHANG Qiu-Ying1,*,LIU Xiao-Bing1   

  1. 1 Key Laboratory of Mollisol Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China;
    2 University of Chinese Academy of Sciences, Beijing 100049, China; 3 Rice Research Institute, Heilongjiang Academy of Land Reclamation Sciences, Jiamusi 154007, China
  • Received:2013-04-17 Revised:2013-06-01 Published:2013-11-12 Published online:2013-08-12
  • Contact: 张秋英, E-mail: zhangqy@neigaehrb.ac.cn, Tel: 0451-86601320

摘要:

田间种植可溶性糖含量不同的3个菜用大豆品种,在R5.5R6R6.2R6.5R7期取样,分析籽粒种皮、子叶和胚轴中蔗糖含量及4种关键酶活性动态,结果表明,籽粒不同部位蔗糖积累呈先增加后下降的趋势,R6.2期是高峰期,此时期品种台292、中科毛豆1号和品系121的胚轴蔗糖含量比子叶分别高57.6%53.6%44.2%;比种皮分别高71.6%75.3%73.6%。由于子叶干重占整粒重90%以上,因此整个籽粒的蔗糖含量主要由子叶决定。子叶的蔗糖磷酸合酶(SPS)活性高于胚轴和种皮,在R6.2期表现更加明显,且蔗糖含量高的品系121子叶中SPS活性高于另外2个品种;蔗糖合酶(SS)在籽粒形成期活性变化呈前期高于后期的趋势,最高值出现在灌浆前期R5.5期胚轴中;两种转化酶活性变化差异较大,中性转化酶(NI)活性一直呈不断下降趋势;籽粒不同部位NI活性无明显差异,而酸性转化酶(AI)活性差异较大;胚轴和子叶中AI活性明显低于种皮,且种皮中AI活性与种皮中蔗糖积累显著负相关(r=0.59)蔗糖积累与4种关键酶活性的相关分析发现,籽粒中蔗糖的含量并非受某一种酶绝对调控,SPS活性与SS+AI+NI活性总和之差与籽粒中蔗糖的积累显著正相关(r=0.53**)

关键词: 蔗糖磷酸合成酶, 光合产物, 菜用大豆, 可溶性糖

Abstract:

Three vegetable soybean varieties with different soluble sugar contents were grown in field condition. Sucrose contents and four critical enzymes activities in different parts of seed were analyzed at R5.5, R6, R6.2, R6.5, and R7 stages, respectively. Sucrose contents of different seed parts increased at early seed filling stage and then decreased at late stage with the peak value at R6.2 stage. Sucrose content in hypocotyl was 57.6%, 53.6%, 44.2% greater than that of cotyledon, and 71.6%, 75.3%, 73.6% greater than that of seed coat at R6.2 stage for the three varieties, respectively. The cotyledon contributed 90% dry weight to the total seed dry weight, and thus the sucrose content of the whole seed was primarily determined by sucrose content in cotyledon. Cotyledon had the highest activity of sucrose phosphate synthase (SPS). The cotyledon SPS activity of cv. 121 was much higher than that of the other two varieties. The sucrose synthase (SS) activity of different seed parts was higher in early seed filling stage than in late filling stage. The highest value of SS activity was observed in hypocotyl at R5.5 stage. Greater differences were found between acid invertase (AI) and neutral invertase (NI) activities. NI activity was higher in early seed filling stage and then decreased till R7 stage. Significant differences were observed for AI activities among three seed parts at the same seed filling stage but there were no differences for NI activities. AI activity in seed coat was much higher than that in hypocotyl and cotyledon. Negative relationship was found between AI activity and sucrose content in seed coat (r= –0.59**). The correlation analysis between sucrose accumulation and four critical enzymes activities showed that seed sucrose content was not controlled dominantly by one enzyme. The difference between sucrose synthesis enzyme activity (SPS) and sucrose decomposition enzyme activity (SS+AI+NI) was positively correlated with seed sucrose accumulation (r=0.53**).

Key words: Sucrose phosphate synthase, Photosynthate, Vegetable soybean, Soluble sugar

[1]Brar G S, Carter T E. Soybean (Glycine max (L.) Merrill): In: Kalloo G, Bergh B O, eds. Genetic Improvement of Vegetable Crops. New York: Pergamon Press, 1993. pp 427–763

[2]Messina M, Kucuk O, Lampe J W. An overview of the health effects of isoflavones with an emphasis on prostate cancer risk and prostate-specific antigen levels. J Aoac Intl, 2006, 89: 1121–1134

[3]Rao M, Bhagsari A, Mohamed A. Fresh green seed yield and seed nutritional traits of vegetable soybean genotypes. Crop Sci, 2002, 42: 1950–1958

[4]Duppong L M, Harlene H V. Yield and quality of vegetable soybean cultivars for production in North Dakota. Horttechnology, 2005, 15: 896–900

[5]Young G, Mebrahtu T, Johnson J. Acceptability of green soybeans as a vegetable entity. Plant Food Human Nutr, 2000, 55: 323–333

[6]Defilippi B G, Manríquez D, Luengwilai K, González-Agüero M. Aroma volatiles: biosynthesis and mechanisms of modulation during fruit ripening. Adv Bot Res, 2009, 50: 1–37

[7]Jouquand C, Chandler C, Plotto A, Gooder K. A sensory and chemical analysis of fresh strawberries over harvest dates and seasons reveals factors that affect eating quality. J Am Soc Hort Sci, 2008, 133: 859–867

[8]Masuda R. Quality requirement and improvement of vegetable soybean. Vegetable Soybean: Proceedings of a workshop, Taiwan. 1991, 92–102

[9]Shanmugasundaram, Yan S R, Yang R Y. Association between protein, oil and sugar in vegetable soybean. Paper presented at the 2nd international vegetable soybean conference. Tacoma: Washington State University, 2001. pp 10–12

[10]Li Y S, Du M, Zhang Q Y, Wang G H, Liu X B. Greater differences exist in seed protein, oil, total soluble sugar and sucrose content of vegetable soybean genotypes [Glycine max (L.) Merrill] in Northeast China. Aust J Crop Sci, 2012, 60: 1681–1686

[11]Liu Y(刘洋), Lin X-H(林希昊), Yao Y-L(姚艳丽), Su J-B(苏俊波). Sucrose metabolism in higher plants. Chin Agric Sci Bull (中国农学通报),2012, 28(6): 145–152 (in Chinese with English abstract)

[12]Yan M-L(闫梅玲), Dai H-J(代红军), Shan S-M(单守明), Wang Z-P(王振平), Fan Y(范永), Zhou M(周明). Research progress of the influence of sucrose-metabolizing enzymes on the sugar accumulation in fruit. J Anhui Agric Sci (安徽农业科学), 2009, 37(29): 14021–14023 (in Chinese with English abstract)

[13]Wang X-Y(王小燕), Wang D(王东), Yu Z-W(于振文). Interactions of water management and nitrogen application on photosynthetic character and kernel yield and nitrogen use efficiency and water use efficiency in wheat. Agric Res Arid Areas (干旱地区农业研究), 2009, 27(6): 17–22 (in Chinese with English abstract)

[14]Chen Y(陈洋), Zhao H-W(赵宏伟). Studies on the accumulation of sucrose in gains of different spring maize. J Northeast Agric Univ (东北农业大学学报), 2007, 38(4): 449–453 (in Chinese with English abstract)

[15]Zhang G-W(张古文), Hu Q-Z(胡齐赞), Xu S-C(徐盛春), Gong Y-M(龚亚明). Study on sucrose accumulation and enzyme activities involved in sucrose metabolism in developing seeds of vegetable soybean. Acta Agric Zhejiangensis (浙江农业学报), 2012, 24(6): 1015–1020 (in Chinese with English abstract)

[16]Ishimaru K, Hirotsu N, Kashiwagi T, Madoka Y, Nagasuga K, Ono K, Ohsugi R. Overexpression of a maize SPS gene improves yield characters of potato under field conditions. Plant Prod Sci, 2008, 11: 104–107

[17]Jang J C, Sheen J. Sugar sensing in higher plants. Plant Cell Online, 1994, 6: 1665–1679

[18]Qin Q-P(秦巧平), Zhang S-L(张上隆), Xie M(谢鸣), Chen J-W(陈俊伟). Progress on the research of the molecular regulation of sugar content and composition in fruit. J Fruit Sci (果树学报), 2005, 22(5): 519–525 (in Chinese with English abstract)

[19]Wang W-J(王文静), Wang G-J(王国杰), Wang Y-H(王永华). Dynamic changes of activities of key enzymes involved in sucrose metabolism during grain filling in wheat and the relationship with starch accumulation in grain. Acta Agron Sin (作物学报), 2007, 33(7): 1122–1128 (in Chinese with English abstract)

[20]Ma C-M(马春梅), Guo H-L(郭海龙), Gong Z-P(龚振平), Xu Y(徐瑶), Wei D(魏丹), Chi F-Q(迟凤琴). The effects of plant growth regulating substances on the reverse sugar beet bolting. Crops (作物杂志), 2012, 150(5): 71–75 (in Chinese with English abstract)

[21]Wang Y-Z(王永章), Wang X-F(王小芳), Zhang D-P(张大鹏). Study of invertase in apple fruit. J China Agric Univ (中国农业大学学报), 2001, 6(5): 9–14 (in Chinese with English abstract)

[22]Sturm A. Invertases primary structures, functions, and roles in plant development and sucrose partitioning. Plant Physiol, 1999, 121: 1–8

[23]Xu D P, Sung S J S, Black C C. Sucrose metabolism in lima bean seeds. Plant Physiol, 1989, 89: 1106–1116

[24]Estruch J J, Beltrán J P. Changes in invertase activities precede ovary growth induced by gibberellic acid in. Physiol Plant, 2006, 81: 319–326

[25]Wang Z-Q(王志琴), Ye Y-X(叶玉秀), Yang J-C(杨建昌), Yuan L-M(袁莉民), Wang X-M(王学明), Zhu Q-S(朱庆森). Changes and regulations of sucrose synthase activity in rice grains during grain filling. Acta Agron Sin (作物学报), 2004, 30(7): 634–643 (in Chinese with English abstract)

[26]Li T(李天), Liu Q-H(刘奇华), Qhsug R(大杉立), Yamagishi T(山岸徹), Sasaki H(佐佐木治人). Effect of high temperature on sucrose content and sucrose-cleaving enzymes activity in rice during grain filling stage. Chin J Rice Sci (中国水稻科学), 2006, 20(6): 626–630 (in Chinese with English abstract)

[27]Kong Q-Q(孙庆泉), Wu Y-Q(吴元奇), Hu C-H(胡昌浩), Dong S-T(董树亭), Rong Y-Z(荣廷昭), Zhang Y(张颖). Physiological activities and multiplication of endosperm cell at filling stage of kernels with different yield potential in maize. Acta Agron Sin (作物学报), 2005, 31(5): 612–618 (in Chinese with English abstract)

[28]MinKuo T, Lowell C A, Smith P T. Changes in soluble carbohydrates and enzymic activities in maturing soybean seed tissues. Plant Sci, 1997, 125: 1–11

[29]Farrar J, Pollock C, Gallagher J. Sucrose and the integration of metabolism in vascular plants. Plant Sci, 2000, 154: 1–11

[30]Zhang Q Y, Gao Q L, Herbert S J, Li Y S, Hashemi A M. Influence of sowing date on phenological stages, seed growth and marketable yield of four vegetable soybean cultivars in Northeastern USA. Afr J Agric Res, 2010, 5: 2556–2562

[31]Sch?fer W E, Rohwer J M, Botha F C. Partial purification and characterisation of sucrose synthase in sugarcane. J Plant Physiol, 2005, 162: 11–20

[32]Koch K. Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Curr Opin Plant Biol, 2004, 7: 235–246

[33]Lu H-Q(卢合全), Shen F-F(沈法富), Liu L-X(刘凌霄), Sun W-F(孙维方). Recent advances in study on plant sucrose synthase. Chin Agric Sci Bull (中国农学通报), 2005, 21(7): 34–37 (in Chinese with English abstract)

[34]Huang D-L(黄东亮), Li S-X(李双喜), Liao Q(廖青), Qin C-X(秦翠鲜), Lin L(林丽), Fang F-X(方锋学), Li Y-R(李杨瑞). Advances on sucrose phosphate synthase in plants. China Biotechnol (中国生物工程杂志), 2012, 32(6): 109–119 (in Chinese with English abstract)

[35]Gan C-X(甘彩霞), Wu C(吴楚). Advances on the studies of 3 key enzymes on sucrose metabolism. J Yangtze Univ (Nat Sci Edn) (长江大学学报?自然科学版), 2007,4 (1): 74–78 (in Chinese with English abstract)

[36]Thorne J H, Rainbird R M. An in vivo technique for the study of phloem unloading in seed coats of developing soybean seeds. Plant Physiol, 1983, 72: 268–271

[37]Liu H-Y(刘慧英), Zhu Z-J(朱祝军). Advances on the studies of invertase on sucrose metabolism in higher plant. Chin Bullf Bot (植物学通报), 2002, 19(6): 666–674 (in Chinese with English abstract)

[38]Pelleschi S, Rocher J P, Prioul J L. Effect of water restriction on carbohydrate metabolism and photosynthesis in mature maize leaves. Plant, Cell Environt, 2008, 20: 493–503

[39]Lee H S, Sturm A. Purification and characterization of neutral and alkaline invertase from carrot. Plant Physiol, 1996, 112: 1513–1522

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