作物学报 ›› 2013, Vol. 39 ›› Issue (11): 2099-2105.doi: 10.3724/SP.J.1006.2013.02099
• 研究简报 • 上一篇
李彦生1,2,南海洋1,2,杜明3,连腾祥1,2,张秋英1,*,刘晓冰1
LI Yan-Sheng1,2,NAN Hai-Yang1,2,DU Ming3,LIAN Teng-Xiang1,2,ZHANG Qiu-Ying1,*,LIU Xiao-Bing1
摘要:
田间种植可溶性糖含量不同的3个菜用大豆品种,在R5.5、R6、R6.2、R6.5和R7期取样,分析籽粒种皮、子叶和胚轴中蔗糖含量及4种关键酶活性动态,结果表明,籽粒不同部位蔗糖积累呈先增加后下降的趋势,R6.2期是高峰期,此时期品种台292、中科毛豆1号和品系121的胚轴蔗糖含量比子叶分别高57.6%、53.6%和44.2%;比种皮分别高71.6%、75.3%和73.6%。由于子叶干重占整粒重90%以上,因此整个籽粒的蔗糖含量主要由子叶决定。子叶的蔗糖磷酸合酶(SPS)活性高于胚轴和种皮,在R6.2期表现更加明显,且蔗糖含量高的品系121子叶中SPS活性高于另外2个品种;蔗糖合酶(SS)在籽粒形成期活性变化呈前期高于后期的趋势,最高值出现在灌浆前期R5.5期胚轴中;两种转化酶活性变化差异较大,中性转化酶(NI)活性一直呈不断下降趋势;籽粒不同部位NI活性无明显差异,而酸性转化酶(AI)活性差异较大;胚轴和子叶中AI活性明显低于种皮,且种皮中AI活性与种皮中蔗糖积累显著负相关(r=–0.59)。蔗糖积累与4种关键酶活性的相关分析发现,籽粒中蔗糖的含量并非受某一种酶绝对调控,SPS活性与SS+AI+NI活性总和之差与籽粒中蔗糖的积累显著正相关(r=0.53**)。
[1]Brar G S, Carter T E. Soybean (Glycine max (L.) Merrill): In: Kalloo G, Bergh B O, eds. Genetic Improvement of Vegetable Crops. New York: Pergamon Press, 1993. pp 427–763[2]Messina M, Kucuk O, Lampe J W. An overview of the health effects of isoflavones with an emphasis on prostate cancer risk and prostate-specific antigen levels. J Aoac Intl, 2006, 89: 1121–1134[3]Rao M, Bhagsari A, Mohamed A. Fresh green seed yield and seed nutritional traits of vegetable soybean genotypes. Crop Sci, 2002, 42: 1950–1958[4]Duppong L M, Harlene H V. Yield and quality of vegetable soybean cultivars for production in North Dakota. Horttechnology, 2005, 15: 896–900[5]Young G, Mebrahtu T, Johnson J. Acceptability of green soybeans as a vegetable entity. Plant Food Human Nutr, 2000, 55: 323–333[6]Defilippi B G, Manríquez D, Luengwilai K, González-Agüero M. Aroma volatiles: biosynthesis and mechanisms of modulation during fruit ripening. Adv Bot Res, 2009, 50: 1–37[7]Jouquand C, Chandler C, Plotto A, Gooder K. A sensory and chemical analysis of fresh strawberries over harvest dates and seasons reveals factors that affect eating quality. J Am Soc Hort Sci, 2008, 133: 859–867[8]Masuda R. Quality requirement and improvement of vegetable soybean. Vegetable Soybean: Proceedings of a workshop, Taiwan. 1991, 92–102[9]Shanmugasundaram, Yan S R, Yang R Y. Association between protein, oil and sugar in vegetable soybean. Paper presented at the 2nd international vegetable soybean conference. Tacoma: Washington State University, 2001. pp 10–12[10]Li Y S, Du M, Zhang Q Y, Wang G H, Liu X B. Greater differences exist in seed protein, oil, total soluble sugar and sucrose content of vegetable soybean genotypes [Glycine max (L.) Merrill] in Northeast China. Aust J Crop Sci, 2012, 60: 1681–1686[11]Liu Y(刘洋), Lin X-H(林希昊), Yao Y-L(姚艳丽), Su J-B(苏俊波). Sucrose metabolism in higher plants. Chin Agric Sci Bull (中国农学通报),2012, 28(6): 145–152 (in Chinese with English abstract)[12]Yan M-L(闫梅玲), Dai H-J(代红军), Shan S-M(单守明), Wang Z-P(王振平), Fan Y(范永), Zhou M(周明). Research progress of the influence of sucrose-metabolizing enzymes on the sugar accumulation in fruit. J Anhui Agric Sci (安徽农业科学), 2009, 37(29): 14021–14023 (in Chinese with English abstract)[13]Wang X-Y(王小燕), Wang D(王东), Yu Z-W(于振文). Interactions of water management and nitrogen application on photosynthetic character and kernel yield and nitrogen use efficiency and water use efficiency in wheat. Agric Res Arid Areas (干旱地区农业研究), 2009, 27(6): 17–22 (in Chinese with English abstract)[14]Chen Y(陈洋), Zhao H-W(赵宏伟). Studies on the accumulation of sucrose in gains of different spring maize. J Northeast Agric Univ (东北农业大学学报), 2007, 38(4): 449–453 (in Chinese with English abstract)[15]Zhang G-W(张古文), Hu Q-Z(胡齐赞), Xu S-C(徐盛春), Gong Y-M(龚亚明). Study on sucrose accumulation and enzyme activities involved in sucrose metabolism in developing seeds of vegetable soybean. Acta Agric Zhejiangensis (浙江农业学报), 2012, 24(6): 1015–1020 (in Chinese with English abstract)[16]Ishimaru K, Hirotsu N, Kashiwagi T, Madoka Y, Nagasuga K, Ono K, Ohsugi R. Overexpression of a maize SPS gene improves yield characters of potato under field conditions. Plant Prod Sci, 2008, 11: 104–107[17]Jang J C, Sheen J. Sugar sensing in higher plants. Plant Cell Online, 1994, 6: 1665–1679[18]Qin Q-P(秦巧平), Zhang S-L(张上隆), Xie M(谢鸣), Chen J-W(陈俊伟). Progress on the research of the molecular regulation of sugar content and composition in fruit. J Fruit Sci (果树学报), 2005, 22(5): 519–525 (in Chinese with English abstract)[19]Wang W-J(王文静), Wang G-J(王国杰), Wang Y-H(王永华). Dynamic changes of activities of key enzymes involved in sucrose metabolism during grain filling in wheat and the relationship with starch accumulation in grain. Acta Agron Sin (作物学报), 2007, 33(7): 1122–1128 (in Chinese with English abstract)[20]Ma C-M(马春梅), Guo H-L(郭海龙), Gong Z-P(龚振平), Xu Y(徐瑶), Wei D(魏丹), Chi F-Q(迟凤琴). The effects of plant growth regulating substances on the reverse sugar beet bolting. Crops (作物杂志), 2012, 150(5): 71–75 (in Chinese with English abstract)[21]Wang Y-Z(王永章), Wang X-F(王小芳), Zhang D-P(张大鹏). Study of invertase in apple fruit. J China Agric Univ (中国农业大学学报), 2001, 6(5): 9–14 (in Chinese with English abstract)[22]Sturm A. Invertases primary structures, functions, and roles in plant development and sucrose partitioning. Plant Physiol, 1999, 121: 1–8[23]Xu D P, Sung S J S, Black C C. Sucrose metabolism in lima bean seeds. Plant Physiol, 1989, 89: 1106–1116[24]Estruch J J, Beltrán J P. Changes in invertase activities precede ovary growth induced by gibberellic acid in. Physiol Plant, 2006, 81: 319–326[25]Wang Z-Q(王志琴), Ye Y-X(叶玉秀), Yang J-C(杨建昌), Yuan L-M(袁莉民), Wang X-M(王学明), Zhu Q-S(朱庆森). Changes and regulations of sucrose synthase activity in rice grains during grain filling. Acta Agron Sin (作物学报), 2004, 30(7): 634–643 (in Chinese with English abstract)[26]Li T(李天), Liu Q-H(刘奇华), Qhsug R(大杉立), Yamagishi T(山岸徹), Sasaki H(佐佐木治人). Effect of high temperature on sucrose content and sucrose-cleaving enzymes activity in rice during grain filling stage. Chin J Rice Sci (中国水稻科学), 2006, 20(6): 626–630 (in Chinese with English abstract)[27]Kong Q-Q(孙庆泉), Wu Y-Q(吴元奇), Hu C-H(胡昌浩), Dong S-T(董树亭), Rong Y-Z(荣廷昭), Zhang Y(张颖). Physiological activities and multiplication of endosperm cell at filling stage of kernels with different yield potential in maize. Acta Agron Sin (作物学报), 2005, 31(5): 612–618 (in Chinese with English abstract)[28]MinKuo T, Lowell C A, Smith P T. Changes in soluble carbohydrates and enzymic activities in maturing soybean seed tissues. Plant Sci, 1997, 125: 1–11[29]Farrar J, Pollock C, Gallagher J. Sucrose and the integration of metabolism in vascular plants. Plant Sci, 2000, 154: 1–11[30]Zhang Q Y, Gao Q L, Herbert S J, Li Y S, Hashemi A M. Influence of sowing date on phenological stages, seed growth and marketable yield of four vegetable soybean cultivars in Northeastern USA. Afr J Agric Res, 2010, 5: 2556–2562[31]Sch?fer W E, Rohwer J M, Botha F C. Partial purification and characterisation of sucrose synthase in sugarcane. J Plant Physiol, 2005, 162: 11–20[32]Koch K. Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Curr Opin Plant Biol, 2004, 7: 235–246[33]Lu H-Q(卢合全), Shen F-F(沈法富), Liu L-X(刘凌霄), Sun W-F(孙维方). Recent advances in study on plant sucrose synthase. Chin Agric Sci Bull (中国农学通报), 2005, 21(7): 34–37 (in Chinese with English abstract)[34]Huang D-L(黄东亮), Li S-X(李双喜), Liao Q(廖青), Qin C-X(秦翠鲜), Lin L(林丽), Fang F-X(方锋学), Li Y-R(李杨瑞). Advances on sucrose phosphate synthase in plants. China Biotechnol (中国生物工程杂志), 2012, 32(6): 109–119 (in Chinese with English abstract)[35]Gan C-X(甘彩霞), Wu C(吴楚). Advances on the studies of 3 key enzymes on sucrose metabolism. J Yangtze Univ (Nat Sci Edn) (长江大学学报?自然科学版), 2007,4 (1): 74–78 (in Chinese with English abstract)[36]Thorne J H, Rainbird R M. An in vivo technique for the study of phloem unloading in seed coats of developing soybean seeds. Plant Physiol, 1983, 72: 268–271[37]Liu H-Y(刘慧英), Zhu Z-J(朱祝军). Advances on the studies of invertase on sucrose metabolism in higher plant. Chin Bullf Bot (植物学通报), 2002, 19(6): 666–674 (in Chinese with English abstract)[38]Pelleschi S, Rocher J P, Prioul J L. Effect of water restriction on carbohydrate metabolism and photosynthesis in mature maize leaves. Plant, Cell Environt, 2008, 20: 493–503[39]Lee H S, Sturm A. Purification and characterization of neutral and alkaline invertase from carrot. Plant Physiol, 1996, 112: 1513–1522 |
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