作物学报 ›› 2015, Vol. 41 ›› Issue (04): 658-665.doi: 10.3724/SP.J.1006.2015.00658
王瑞1,张福耀1,*,程庆军1,田承华1,凌亮2
WANG Rui1,ZHANG Fu-Yao1,*,CHENG Qing-Jun1,TIAN Cheng-Hua1,LING Liang2
摘要:
为建立高粱种子纯度及真实性鉴定技术体系, 构建高粱栽培品种DNA指纹图谱数据库是必要的。利用SSR荧光标记技术筛选出36对SSR荧光标记引物,构建我国高粱杂种优势利用以来中晚熟区主推的20个品种的SSR指纹图谱。36对SSR引物共扩增出235个等位基因,平均每个等位基因检测到多态性位点7个,多态性位点变化范围为2~12个。20个高粱品种36个SSR位点的遗传多样性指数和多态性信息量的变化范围分别为0.3490~0.8813和0.3144~0.8696,平均值分别为0.6976和0.6571。从36对SSR引物中筛选到多态性丰富的2对引物作为高粱品种鉴定的特征引物,2对SSR特征引物组合可区分所有供试品种。20个高粱品种的SSR指纹图谱互不相同,可以作为各品种特定的图谱,为品种鉴别提供依据。
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