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作物学报

作物学报 ›› 2020, Vol. 46 ›› Issue (6): 889-901.doi: 10.3724/SP.J.1006.2020.94150

• 耕作栽培·生理生化 • 上一篇    下一篇

高粱种子对萌发温度的响应分析与耐低温萌发能力鉴定

张瑞栋1,2,肖梦颖1,徐晓雪1,姜冰1,邢艺凡1,陈小飞1,李邦1,艾雪莹1,周宇飞1,*(),黄瑞冬1   

  • 收稿日期:2019-10-08 接受日期:2020-01-15 出版日期:2020-06-12 网络出版日期:2020-01-23
  • 通讯作者: 周宇飞
  • 作者简介:E-mail: sxnkyzrd@126.com
  • 基金资助:
    国家现代农业产业技术体系建设专项(CARS-06-135-A17)

Responses of sorghum hybrids to germination temperatures and identification of low temperature resistance

ZHANG Rui-Dong1,2,XIAO Meng-Ying1,XU Xiao-Xue1,JIANG Bing1,XING Yi-Fan1,CHEN Xiao-Fei1,LI Bang1,AI Xue-Ying1,ZHOU Yu-Fei1,*(),HUANG Rui-Dong1   

  • Received:2019-10-08 Accepted:2020-01-15 Published:2020-06-12 Published online:2020-01-23
  • Contact: Yu-Fei ZHOU
  • Supported by:
    Earmarked Fund for the China Agriculture Research System(CARS-06-135-A17)

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摘要：

萌发期的低温是限制种子萌发的一个重要非生物胁迫因子, 高粱种子耐低温萌发能力是保证高粱出苗整齐、建立良好群体的基础, 因此研究高粱品种对不同萌发温度的响应特征具有重要的理论和应用价值。本试验用来源于不同地区的30份高粱品种, 在25℃、20℃、16℃和12℃人工气候箱中进行萌发试验, 测定不同温度下高粱品种的发芽势、发芽率、芽长、根长、芽重、根重等萌发指标, 分析不同高粱品种的萌发差异; 通过主成分分析和聚类分析, 对不同高粱品种萌发期耐低温特性鉴定和分类, 结果表明, 随着萌发温度的降低, 高粱芽和根的生长均受到抑制, 但根与芽的重量和长度比均增加, 说明萌发过程中芽比根对低温更加敏感。30个高粱品种的6个萌发指标相对值之间存在一定相关性。主成分分析表明, 相对芽长、相对根长和相对发芽率分别在3个主成分中载荷较大, 可作为高粱萌发期耐低温的主要鉴定指标。30个高粱品种按萌发期耐低温能力可分为四大类, 辽粘3号极不耐低温, 济粱1号等18个品种对低温敏感, 冀酿1号等5个品种对低温不敏感, 赤杂101等6个品种对萌发期低温具有较强的耐性。

关键词: 高粱, 萌发特征, 低温敏感性, 主成分分析

Abstract:

Low temperature during germination is an important abiotic stress factor limiting seed germination. Sorghum seeds with high germination capacity at low temperature are conducive to the emergence and establishment of a good population. Therefore, studying the response characteristics of sorghum hybrids to different germination temperatures is of great theoretical and practical value. Thirty sorghum hybrids from different regions of China were germinated in the artificial climate chamber, at 25°C, 20°C, 16°C, or 12°C to measure germination potential, germination percent, bud length, root length, bud weight and root weight. Sorghum hybrids were identified and classified according to low temperature resistance during the germination based on principal component analysis (PCA) and clustering analysis. With a decrease in germination temperature, the growth of both buds and roots were inhibited, but the ratio of roots to buds in both weight and length increased, indicating that buds were more sensitive to low temperature during germination than roots. The relative values of six germination indicators of the thirty sorghum hybrids were correlated between each other. The PCA results showed that relative bud length, relative root length and relative germination percent could be used to evaluate the sorghum germination capacity under low temperatures because these indicators had the largest loads in the three principal components, respectively. According to the low temperature tolerance ability of the 30 sorghum hybrids were divided into four categories: Liaonian 3 was extremely sensitive to low temperature; eighteen hybrids, such as Jiliang 1, were sensitive to low temperature; five hybrids, such as Jiniang 1, were insensitive to low temperature, and six hybrids, such as Chiza 101 had a strong low temperature tolerance during the germination.

Key words: sorghum, germination traits, low temperature sensitivity, principal components analysis

表1

供试高粱品种及来源"

编号
Serial number		 名称
Cultivar		 品种来源
Origin
S1 济粱1号 Jiliang 1 山东省农业科学院Shandong Academy of Agricultural Sciences
S2 济粱2号 Jiliang 2 山东省农业科学院Shandong Academy of Agricultural Sciences
S3 冀酿1号 Jiniang 1 河北省农林科学院Hebei Academy of Agricultural and Forestry Sciences
S4 冀酿2号 Jiniang 2 河北省农林科学院Hebei Academy of Agricultural and Forestry Sciences
S5 辽粘3号 Liaonian 3 辽宁省农业科学院Liaoning Academy of Agricultural Sciences
S6 辽糯11 Liaonuo 11 辽宁省农业科学院Liaoning Academy of Agricultural Sciences
S7 辽杂19 Liaoza 19 辽宁省农业科学院Liaoning Academy of Agricultural Sciences
S8 辽杂27 Liaoza 27 辽宁省农业科学院Liaoning Academy of Agricultural Sciences
S9 辽杂36 Liaoza 36 辽宁省农业科学院Liaoning Academy of Agricultural Sciences
S10 辽杂37 Liaoza 37 辽宁省农业科学院Liaoning Academy of Agricultural Sciences
S11 13163A/早21R 13163A/Zao 21R 四川省农业科学院Sichuan Academy of Agricultural Sciences
S12 3401A/早21R 3401A/Zao 21R 四川省农业科学院Sichuan Academy of Agricultural Sciences
S13 吉杂127 Jiza 127 吉林省农业科学院Jilin Academy of Agricultural Sciences
S14 吉杂136 Jiza 136 吉林省农业科学院Jilin Academy of Agricultural Sciences
S15 吉杂137 Jiza 137 吉林省农业科学院Jilin Academy of Agricultural Sciences
S16 吉杂145 Jiza 145 吉林省农业科学院Jilin Academy of Agricultural Sciences
S17 吉杂152 Jiza 152 吉林省农业科学院Jilin Academy of Agricultural Sciences
S18 吉杂305 Jiza 305 吉林省农业科学院Jilin Academy of Agricultural Sciences
S19 通杂108 Tongza 108 通辽市农业科学研究院Tongliao Academy of Agricultural Sciences
S20 通杂137 Tongza 137 通辽市农业科学研究院Tongliao Academy of Agricultural Sciences
S21 407/272 四川省农业科学院Sichuan Academy of Agricultural Sciences
S22 407/M5 四川省农业科学院Sichuan Academy of Agricultural Sciences
S23 锦杂107 Jinza 107 锦州市科学技术研究院Jinzhou Academy of Science and Technology
S24 锦杂109 Jinza 109 锦州市科学技术研究院Jinzhou Academy of Science and Technology
S25 晋中0592 Jinzhong 0592 山西省农业科学院Shanxi Academy of Agricultural Sciences
S26 晋中0742 Jinzhong 0742 山西省农业科学院Shanxi Academy of Agricultural Sciences
S27 晋杂31 Jinza 31 山西省农业科学院Shanxi Academy of Agricultural Sciences
S28 晋杂33 Jinza 33 山西省农业科学院Shanxi Academy of Agricultural Sciences
S29 平试13 Pingshi 13 平凉市农业科学院Pingliang Academy of Agricultural Sciences
S30 赤杂101 Chiza 101 赤峰市农牧科学院Chifeng Academy of Agricultural and Animal Husbandry Sciences

表2

不同温度下高粱品种萌发指标的差异比较"

品种
Hybrid		 温度
Temperature
(℃)		 发芽势
Germination potential (%)		 发芽率
Germination percent (%)		 芽长
Bud length
(cm)		 根长
Root length
(cm)		 芽重
Bud FW
(mg)		 根重
Root FW
(mg)
S1 25 78.67±6.11 84.90±4.29 9.90±0.93 13.17±2.38 92.00±7.77 72.17±7.96
20 73.33±6.11 84.67±2.31 4.92±0.62 12.80±1.65 54.50±10.48 76.17±11.89
16 33.67±4.51 82.33±6.03 3.35±0.31 6.60±0.88 25.00±3.10 27.50±3.02
12 0.00±0.00 74.67±12.86 0.32±0.04 1.19±0.17 1.83±0.26 8.17±0.75
S2 25 81.33±2.31 84.67±2.52 10.37±1.51 9.00±1.46 89.67±13.17 25.00±4.94
20 80.57±3.44 84.33±4.04 6.02±0.90 15.42±2.80 62.50±6.12 64.03±12.69
16 63.33±3.06 80.00±2.00 2.02±0.16 7.52±0.72 20.60±3.83 63.20±9.45
12 0.00±0.00 72.00±4.00 0.42±0.04 1.45±0.14 2.50±0.46 11.50±1.52
S3 25 68.00±4.00 89.33±2.31 9.03±0.65 13.60±2.05 75.00±8.29 74.33±14.62
20 65.33±7.02 88.00±3.46 5.77±0.64 12.52±1.42 57.17±5.91 65.67±9.58
16 21.33±1.53 87.33±3.06 1.98±0.21 6.85±0.90 22.00±2.90 37.50±5.99
12 0.00±0.00 84.00±6.93 0.37±0.08 1.40±0.13 2.33±0.29 10.67±1.21
S4 25 60.00±4.00 78.33±6.03 9.47±1.16 6.62±0.66 70.67±8.82 17.17±1.47
20 58.67±2.31 76.00±5.29 5.18±0.73 10.18±0.83 55.17±4.58 57.67±9.58
16 37.33±2.31 68.23±3.28 1.86±0.14 4.92±0.68 21.80±4.23 44.80±8.86
12 0.00±0.00 38.67±5.03 0.40±0.06 1.08±0.12 1.50±0.41 6.50±0.55
S5 25 72.00±4.00 74.00±4.00 11.53±1.20 14.47±1.98 106.17±15.78 64.67±7.17
20 31.33±1.53 66.67±2.31 4.40±0.46 7.83±1.16 49.50±5.24 40.00±6.63
16 0.00±0.00 61.33±8.33 2.10±0.29 4.62±0.45 24.00±4.15 26.33±3.27
12 0.00±0.00 10.67±4.62 0.10±0.01 0.72±0.13 0.48±0.15 3.33±0.52
S6 25 93.33±4.62 94.67±2.31 10.97±0.96 14.80±2.23 102.00±14.64 59.33±11.45
20 84.00±5.29 93.67±2.08 5.10±0.28 10.58±1.66 64.83±8.47 56.00±4.43
16 22.67±2.31 93.33±4.62 2.47±0.16 5.67±0.75 28.67±4.18 27.83±4.22
12 0.00±0.00 81.33±4.62 0.27±0.05 1.27±0.16 2.00±0.52 8.33±1.03
S7 25 86.00±4.00 95.33±1.15 11.80±0.98 9.83±1.05 108.58±14.56 19.17±3.13
20 81.33±8.33 94.67±1.53 6.68±0.26 11.73±2.10 72.67±10.89 52.50±7.82
16 80.00±4.00 94.33±3.21 2.28±0.21 6.72±1.12 22.20±2.79 27.40±5.00
12 0.00±0.00 88.00±4.00 0.63±0.12 2.40±0.42 4.17±0.75 12.00±0.89
S8 25 97.33±2.31 98.67±2.31 11.13±1.41 8.23±1.37 77.50±15.14 21.17±3.37
20 92.23±5.08 95.67±3.21 5.77±0.71 13.78±1.50 46.50±5.68 55.83±7.65
16 82.00±3.46 92.67±3.06 1.92±0.07 6.26±0.66 15.20±1.60 31.40±3.72
12 0.00±0.00 90.67±2.31 0.40±0.06 1.65±0.10 1.17±0.41 7.00±0.89
S9 25 84.43±5.10 93.33±2.31 9.10±0.94 13.65±1.96 99.00±14.76 38.67±4.59
20 68.90±6.97 93.33±2.31 8.70±1.28 15.02±1.99 75.67±6.98 64.83±5.27
16 18.67±1.53 92.00±4.00 2.40±0.20 6.35±0.86 32.17±4.92 40.83±6.74
12 0.00±0.00 90.00±2.00 0.67±0.08 1.93±0.23 7.83±1.33 14.33±2.42
S10 25 73.33±5.03 90.67±2.31 11.45±0.98 13.88±2.72 85.33±10.19 62.67±3.61
20 53.33±3.06 89.33±2.31 5.72±0.74 11.58±2.17 52.33±6.56 56.17±8.35
16 33.33±4.16 86.67±4.62 2.65±0.26 6.98±1.05 27.17±4.75 28.83±0.75
12 0.00±0.00 58.67±8.33 0.17±0.05 1.27±0.23 1.33±0.39 7.00±1.10
S11 25 61.33±6.11 85.33±2.31 8.92±0.77 7.70±1.00 81.83±6.21 33.33±4.32
20 58.00±6.00 82.67±2.31 5.60±0.49 10.67±1.35 54.50±6.66 61.50±10.56
16 12.33±1.53 80.00±4.00 2.45±0.19 5.30±0.83 24.67±3.67 30.17±3.60
S11 12 0.00±0.00 78.67±2.31 0.35±0.08 2.05±0.18 1.33±0.34 11.67±1.63
S12 25 84.00±2.43 89.33±2.31 10.13±0.90 14.78±2.05 111.00±18.22 50.00±7.95
20 61.33±5.13 86.67±2.31 6.98±1.01 9.73±1.41 82.33±8.24 56.00±10.08
16 27.33±2.31 84.00±4.00 2.10±0.26 5.67±0.81 27.33±4.13 36.00±5.73
12 0.00±0.00 81.33±2.31 0.68±0.10 2.10±0.22 6.17±1.17 13.33±2.07
S13 25 87.33±5.03 89.33±3.06 8.97±1.34 13.22±2.28 78.17±13.47 44.50±6.09
20 78.00±5.29 84.67±3.06 5.87±0.48 11.52±2.09 59.33±9.93 66.67±3.33
16 5.33±0.58 82.67±2.31 2.03±0.24 5.20±0.89 24.50±4.32 38.67±3.39
12 0.00±0.00 52.00±4.00 0.10±0.00 1.02±0.19 0.55±0.26 6.50±1.05
S14 25 82.00±4.00 93.33±2.31 11.22±1.39 14.00±1.52 112.17±16.86 56.67±9.27
20 73.33±6.11 90.67±2.31 6.18±0.39 10.88±1.19 76.33±9.97 60.17±11.32
16 46.00±4.00 80.00±3.46 2.17±0.19 6.60±0.54 25.00±2.53 33.17±3.06
12 0.00±0.00 62.00±2.00 0.38±0.12 1.97±0.27 3.17±0.88 11.33±2.16
S15 25 90.67+2.31 93.33±2.31 9.37±0.81 14.48±1.23 76.67±4.59 52.83±6.91
20 89.33+2.31 90.67±2.31 5.83±0.66 13.25±1.46 58.33±10.13 64.67±8.36
16 16.00±1.00 90.67±6.11 2.13±0.15 6.68±1.02 25.33±4.72 33.67±5.65
12 0.00±0.00 70.67±4.62 0.27±0.08 1.17±0.20 2.17±0.75 7.17±0.75
S16 25 74.67±8.32 89.33±2.31 9.33±1.05 14.80±2.21 88.44±6.62 51.44±9.39
20 72.00±9.17 81.33±6.11 5.25±0.33 14.20±2.62 52.17±5.56 67.67±6.53
16 42.67±5.03 80.67±7.57 1.68±0.12 8.93±1.28 17.67±1.86 47.33±7.97
12 0.00±0.00 56.00±4.00 0.45±0.10 2.10±0.25 2.50±0.49 12.17±0.75
S17 25 78.67±6.11 86.67±2.31 12.42±0.69 9.92±1.21 101.67±12.86 27.33±4.03
20 73.23±5.85 82.67±4.38 7.22±0.63 14.75±1.88 72.50±11.13 75.5±12.63
16 58.90±3.44 78.67±2.31 2.14±0.34 5.96±0.79 21.40±4.03 54.40±8.45
12 0.00±0.00 58.00±6.00 0.50±0.13 1.43±0.16 3.33±1.03 9.00±1.55
S18 25 78.67±8.33 89.33±2.31 11.83±1.11 13.78±2.15 108.67±12.14 62.50±9.46
20 62.67±6.11 88.00±4.00 5.53±0.38 9.50±1.37 62.00±7.01 56.50±9.73
16 14.67±1.53 81.33±6.11 2.38±0.36 6.55±0.27 32.17±6.21 36.33±5.20
12 0.00±0.00 68.00±6.93 0.43±0.05 1.55±0.19 2.83±0.75 9.50±1.87
S19 25 63.33±5.03 85.33±4.62 8.28±0.54 12.95±2.42 74.67±11.52 50.33±5.09
20 56.00±8.00 72.67±5.03 7.50±0.93 15.45±2.27 77.17±12.91 67.67±8.94
16 17.33±1.53 66.00±5.29 2.07±0.26 6.60±1.25 21.00±3.03 31.50±6.09
12 0.00±0.00 62.00±6.00 0.25±0.05 1.53±0.12 1.83±0.68 7.33±1.03
S20 25 86.67±6.65 88.00±8.00 9.28±1.25 15.87±2.83 98.33±7.60 32.67±1.32
20 66.00±7.21 80.00±4.00 6.40±0.92 15.38±2.63 78.00±7.62 82.00±10.71
16 40.00±5.29 77.33±3.06 1.97±0.14 6.95±1.09 24.00±2.28 35.33±4.13
12 0.00±0.00 74.00±2.00 0.40±0.06 2.37±0.35 3.83±1.03 15.50±1.87
S21 25 86.67±6.11 94.67±2.31 9.27±1.04 7.70±1.04 87.28±9.61 27.33±4.63
20 80.80±3.73 93.33±4.62 5.98±0.67 12.83±1.96 58.00±11.24 60.65±7.38
16 72.67±5.03 90.67±6.11 2.50±0.28 7.12±0.44 26.20±3.19 56.47±5.18
12 0.00±0.00 89.33±2.31 0.30±0.06 1.32±0.12 2.33±0.45 6.50±1.05
S22 25 54.67±8.08 69.33±4.62 11.02±1.43 14.17±2.24 102.00±18.19 100.67±16.85
20 36.67±3.06 66.67±6.11 6.20±0.46 10.97±1.65 67.33±7.00 68.83±6.52
16 8.00±1.00 65.33±2.31 1.57±0.20 4.58±0.52 16.17±1.83 27.67±2.07
S22 12 0.00±0.00 61.33±8.33 0.23±0.05 1.52±0.16 1.17±0.27 9.00±0.63
S23 25 91.33±4.16 96.67±1.15 9.95±1.34 15.23±2.92 73.00±10.77 46.33±6.92
20 90.67±4.62 96.00±4.00 5.40±0.25 12.43±1.26 45.83±3.82 58.17±4.88
16 13.33±1.53 94.67±2.31 2.48±0.44 6.85±1.07 40.67±6.19 33.83±6.18
12 0.00±0.00 52.00±8.00 0.22±0.04 0.95±0.15 3.67±0.82 5.50±0.55
S24 25 76.67±5.03 85.33±2.31 9.83±1.14 8.75±1.49 79.50±12.60 40.50±7.69
20 66.67±6.11 81.33±5.77 5.68±0.62 7.72±1.26 54.50±7.37 40.67±7.34
16 16.00±1.73 77.33±8.33 2.05±0.21 5.67±0.48 25.83±2.48 24.33±4.68
12 0.00±0.00 54.00±2.00 0.23±0.05 1.13±0.22 1.17±0.41 7.50±1.05
S25 25 68.33±2.52 89.33±2.31 10.90±0.51 16.05±2.61 89.67±7.55 84.67±9.14
20 64.00±6.00 89.33±6.11 6.22±0.58 14.17±1.87 64.50±6.44 98.50±18.25
16 60.00±3.46 86.67±6.11 2.68±0.19 7.68±0.61 33.00±2.53 46.17±5.81
12 0.00±0.00 57.33±4.62 0.15±0.05 1.25±0.08 1.58±0.49 8.83±1.17
S26 25 89.33±8.33 92.00±4.00 9.35±1.13 16.28±3.11 74.67±11.2 67.33±11.93
20 65.33±6.43 90.67±6.11 4.85±0.71 10.35±1.23 48.00±9.23 59.00±5.59
16 36.00±1.73 89.33±2.31 2.62±0.30 6.18±0.73 27.00±3.41 32.33±6.02
12 0.00±0.00 77.33±8.33 0.13±0.05 0.95±0.08 0.67±0.22 6.83±0.75
S27 25 90.67±2.31 93.33±2.31 8.30±1.08 15.13±2.06 95.17±11.34 51.00±9.84
20 89.33±2.31 89.33±6.11 7.35±1.08 16.13±2.32 98.50±7.92 78.17±8.66
16 62.00±6.00 88.67±3.06 2.27±0.20 8.07±0.79 28.67±3.93 46.00±7.40
12 0.00±0.00 38.00±2.00 0.55±0.05 2.28±0.29 3.50±0.84 12.17±1.47
S28 25 84.00±10.58 88.00±6.93 11.87±1.37 16.00±1.11 94.83±15.46 61.33±4.72
20 60.67±6.11 86.67±6.11 6.20±0.87 12.07±2.01 68.67±10.78 67.67±11.31
16 36.00±2.00 85.33±6.11 2.63±0.30 6.83±1.04 30.83±5.12 35.50±6.12
12 0.00±0.00 78.67±2.31 0.38±0.10 1.93±0.34 2.83±0.71 11.00±1.67
S29 25 82.67±4.16 88.00±4.00 10.73±1.54 8.62±1.40 80.83±10.32 32.67±4.80
20 80.57±3.44 84.00±6.93 5.15±0.34 10.85±1.03 39.33±4.89 47.83±7.47
16 73.33±2.31 82.67±4.62 1.76±0.14 6.44±0.83 15.20±2.48 35.20±3.19
12 0.00±0.00 66.67±6.11 0.25±0.05 1.65±0.27 1.33±0.41 8.33±1.03
S30 25 83.33±7.02 86.67±6.11 9.40±0.73 9.62±1.06 93.00±8.10 20.67±3.78
20 78.30±5.00 85.33±8.33 6.25±0.86 16.78±2.67 69.17±8.06 86.17±16.46
16 70.00±8.00 84.00±4.00 2.16±0.24 7.94±1.04 23.00±3.90 45.20±7.08
12 0.00±0.00 56.00±4.00 0.57±0.08 1.93±0.37 5.67±1.21 14.33±1.63
品种
Hybrid(H)		 F-value 42.97 42.84 7.28 16.59 16.3 24.31
P-value ** ** ** ** ** **
处理Treatment(T) F value 3919.17 397.94 7772.28 2495.72 4756.06 2039.14
P-value ** ** ** ** ** **
H×T F value 17.42 7.62 8.09 10.44 7.08 21.35
P-value ** ** ** ** ** **
变异系数CV (%) 74.29 17.34 82.17 60.62 78.02 59.88

表3

温度对发芽指标的影响"

性状 Trait 25℃ 20℃ 16℃ 12℃
发芽势 Germination potential (%) 79.64±11.73 a 69.73±15.26 b 37.32±24.57 c 0.00±0.00 d
发芽率 Germination percent (%) 88.55±6.92 a 85.63±8.38 b 82.85±9.34 c 66.07±18.19 d
芽长 Bud length (cm) 10.17±1.15 a 6.00±0.90 b 2.23±0.36 c 0.35±0.16 d
根长 Root length (cm) 12.54±3.01 a 12.38±2.39 a 6.48±1.01 b 1.55±0.46 c
芽重 Bud FW (mg) 90.25±12.66 a 62.58±12.88 b 24.95±5.56 c 2.56±1.70 d
根重 Root FW (mg) 48.28±20.66 b 63.76±12.48 a 37.16±9.57 c 9.41±2.96 d
根芽比(长度) Root/bud (length) 1.01±0.32 d 1.91±0.33 c 2.62±0.83 b 5.01±1.79 a
根芽比(鲜重) Root/bud (FW) 0.54±0.23 c 1.04±0.21 bc 1.57±0.56 b 4.80±2.41 a

表4

不同高粱品种萌发特性相对值"

品种
Hybrid		 X1 X2 X3 X4 X5 X6
S1 31.21±4.75 gh 92.95±5.53 ac 22.01±1.03 ce 39.40±3.01 fi 20.91±2.07 kn 37.66±1.90 no
S2 44.30±1.43 ad 90.86±1.26 ad 19.26±1.07 fj 67.62±3.94 a 21.97±1.09 ik 155.08±9.07 b
S3 28.01±6.37 hk 96.05±2.89 a 21.04±1.02 cf 39.17±4.17 gi 25.39±0.82 dg 40.46±3.09 mn
S4 38.89±0.93 df 70.77±1.03 ij 18.76±1.07 gk 61.19±2.16 bc 25.75±0.58 df 166.71±16.62 b
S5 8.02±1.12 o 51.71±4.40 k 13.56±0.22 m 23.08±0.79 m 16.41±0.45 o 27.46±1.81 o
S6 24.75±2.18 il 92.52±2.05 ac 17.31±0.55 jl 30.19±1.25 kl 22.10±0.64 hk 40.37±3.71 mn
S7 48.49±1.16 a 95.80±2.53 a 19.53±0.69 fi 56.33±4.38 cd 21.05±0.58 kn 128.91±4.45 c
S8 45.63±1.70 ac 93.50±0.82 ac 17.14±0.90 kl 66.63±4.64 ab 18.68±2.28 mo 115.11±9.18 de
S9 22.42±1.76 kn 97.80±1.26 a 30.00±1.66 a 43.07±1.58 eg 29.08±3.20 bc 84.01±2.02 f
S10 28.68±1.97 hj 81.23±5.42 eh 17.63±0.73 il 36.84±3.05 hj 22.66±1.11 gk 37.25±1.64 no
S11 23.71±4.95 jm 93.58±2.40 ac 22.67±0.42 c 61.45±2.31 bc 23.08±1.55 fk 81.16±4.36 fg
S12 24.28±4.70 il 93.14±1.23 ac 22.84±1.23 c 31.90±1.65 jl 24.77±1.52 di 57.60±2.66 ik
S13 18.55±0.78 mn 76.40±0.97 hi 20.35±1.17 eh 32.95±1.63 jl 24.85±1.39 dh 64.16±4.33 hj
S14 35.22±1.28 fg 78.54±1.01 fh 18.38±1.02 hk 36.93±1.65 hj 21.47±1.09 km 48.95±1.77 kn
S15 24.11±1.45 im 86.79±2.79 ce 20.49±1.20 dg 36.09±1.70 hj 26.34±3.33 ce 50.41±1.61 km
S16 36.88±0.51 ef 77.09±4.68 hi 18.81±0.72 gk 44.74±1.13 ef 18.90±0.70 lo 67.06±4.82 hi
S17 42.23±0.79 be 80.10±3.23 eh 18.39±1.21 gk 54.58±3.14 d 21.70±1.17 jl 133.62±13.08 c
S18 20.87±2.42 ln 85.17±4.50 dg 17.14±0.80 kl 34.40±3.22 hk 21.64±1.38 jl 43.52±2.78 ln
S19 25.32±3.55 il 76.47±3.18 hi 26.48±2.07 b 45.02±2.72 e 29.70±1.69 b 52.56±3.38 jl
S20 29.43±1.50 hi 86.91±4.83 be 22.03±1.90 ce 39.86±2.25 eh 24.66±0.93 di 105.15±9.62 e
S21 45.24±0.81 ac 95.60±2.08 a 22.51±0.97 cd 70.31±5.17 a 23.49±1.43 ek 122.58±9.24 cd
S22 17.35±1.15 n 91.74±3.24 ad 16.25±0.73 l 30.43±1.28 kl 18.30±1.88 no 26.45±2.51 o
S23 23.19±2.03 jm 76.91±4.72 hi 19.48±0.88 fi 33.61±3.57 jk 32.70±1.20 a 53.46±1.37 jl
S24 22.95±2.69 kn 78.29±3.76 gh 18.84±1.09 gk 44.66±4.60 eg 24.42±1.94 dj 47.66±2.84 kn
S25 47.03±2.22 ab 81.72±4.59 eh 19.41±1.12 fj 36.47±3.29 hj 26.44±1.21 cd 44.58±2.38 ln
S26 26.94±1.76 hk 91.03±2.44 ad 19.60±0.82 fi 27.61±2.67 lm 24.39±1.28 dj 37.45±2.57 no
S27 41.02±1.86 ce 68.97±1.86 j 28.75±1.22 a 44.89±1.61 ef 31.04±1.15 ab 70.68±5.12 gh
S28 27.6±1.99 hk 93.78±4.45 ab 18.61±1.25 gk 33.86±3.30 ik 25.69±1.49 df 48.11±4.41 kn
S29 47.65±1.22 ab 85.40±2.34 df 15.62±1.33 lm 57.83±3.06 cd 16.06±0.85 o 75.70±3.67 fh
S30 45.37±0.57 ac 81.67±0.63 eh 22.81±1.40 c 69.31±3.67 a 24.85±1.47 dh 184.80±13.69 a
平均值 Mean 31.51 84.75 20.19 44.35 23.62 74.96
变异范围 Range 8.02-48.49 51.71-97.80 13.56-30.00 23.08-70.31 16.06-32.70 26.45-184.80
变异系数
CV (%)		 34.40 12.07 17.77 30.75 16.77 58.36

表5

萌发期低温胁迫下各性状的相关系数"

萌发指标
Germination traits		 X1 X2 X3 X4 X5 X6
X1 1
X2 0.20 1
X3 0.06 0.20 1
X4 0.74** 0.21 0.17 1
X5 -0.09 -0.02 0.71** -0.10 1
X6 0.66** 0.08 0.17 0.85** 0.01 1

附表1

3个主成分的特征值及贡献率"

主成分
Principal component		 特征值
Eigen value		 贡献率
Contribution (%)		 累计贡献率
Cumulative contribution (%)
I 2.59 43.23 43.23
II 1.71 28.56 71.79
III 0.97 16.21 87.99

附表2

各因子载荷矩阵"

萌发指标
Germination traits		 主成分I
Principal I		 主成分II
Principal II		 主成分III
Principal III
X1 0.854 -0.166 -0.025
X2 0.307 0.136 0.935
X3 0.272 0.897 0.030
X4 0.943 -0.112 -0.062
X5 0.010 0.921 -0.193
X6 0.899 -0.053 -0.237

附表3

因子得分系数矩阵"

萌发指标
Germination traits		 主成分I
Principal I		 主成分II
Principal II		 主成分III
Principal III
X1 0.329 -0.097 -0.026
X2 0.118 0.079 0.961
X3 0.105 0.523 0.031
X4 0.363 -0.065 -0.064
X5 0.004 0.538 -0.198
X6 0.346 -0.031 -0.244

附表4

各品种综合因子得分Y 值及耐低温特性的排序"

编号
Serial number		 Y1 Y2 Y3 Y 排序
Order
S1 0.510 0.234 0.734 0.461 16
S2 1.058 0.156 0.403 0.644 2
S3 0.512 0.257 0.748 0.473 14
S4 1.033 0.164 0.179 0.593 6
S5 0.282 0.169 0.385 0.264 30
S6 0.460 0.226 0.727 0.433 22
S7 0.946 0.168 0.522 0.615 4
S8 0.921 0.141 0.532 0.596 5
S9 0.670 0.315 0.653 0.551 8
S10 0.473 0.215 0.619 0.416 28
S11 0.718 0.229 0.617 0.540 9
S12 0.531 0.264 0.686 0.473 13
S13 0.516 0.241 0.509 0.425 26
S14 0.533 0.200 0.566 0.431 23
S15 0.511 0.255 0.636 0.451 20
S16 0.628 0.175 0.508 0.459 18
S17 0.915 0.158 0.361 0.567 7
S18 0.464 0.217 0.647 0.418 27
S19 0.549 0.289 0.521 0.459 17
S20 0.733 0.230 0.504 0.527 10
S21 0.967 0.192 0.524 0.634 3
S22 0.386 0.211 0.762 0.398 29
S23 0.497 0.278 0.523 0.430 24
S24 0.516 0.226 0.559 0.430 25
S25 0.560 0.225 0.595 0.458 19
S26 0.448 0.250 0.717 0.433 21
S27 0.656 0.281 0.399 0.487 12
S28 0.512 0.246 0.710 0.462 15
S29 0.747 0.128 0.560 0.512 11
S30 1.163 0.171 0.236 0.670 1

表6

不同高粱品种耐低温特性聚类结果"

温度敏感类别
Type of temperature-sensitivity		 品种
Hybrid
高度低温敏感Highly cold-sensitive S5
低温敏感Cold-sensitive S1, S3, S6, S10, S12, S13, S14, S15, S16, S18, S19, S22, S23, S24, S25, S26, S27, S28
低温不敏感Cold-tolerance S9, S11, S17, S19, S20
低温极不敏感Highly cold-tolerance S2, S4, S7, S8, S21, S30
[1] Huang R D . Research progress on plant tolerance to soil salinity and alkalinity in sorghum. J Integr Agric, 2018,17:739-746.
[2] Tiryaki I, Andrews D J . Germination and seedling cold tolerance in sorghum: II. Parental lines and hybrids. Agron J, 2001,93:1391-1397.
doi: 10.2134/agronj2001.1391
[3] Peacock J M. Response and tolerance of sorghum to temperature stress. In: House L R, Mughogho L K, Peacock J M, eds. Sorghum in the Eighties Proceedings of the International Symposium on Sorghum. Patancheru, India: International Crops Research Institute for the Semi-Arid Tropics. 1982. pp 143-159.
[4] Yu J, Tuinstra M R . Genetic analysis of seedling growth under cold temperature stress in grain sorghum. Crop Sci, 2001,41:1438-1443.
[5] Bekele W A, Fiedler K, Shiringani A, Schnaubelt D, Windpassinger S, Uptmoor R, Friedt W, Snowdon R J . Unravelling the genetic complexity of sorghum seedling development under low-temperature conditions. Plant Cell Environ, 2014,37:707-723.
[6] Ma Z, Bykova N V, Igamberdiev A U . Cell signaling mechanisms and metabolic regulation of germination and dormancy in barley seeds. Crop J, 2017,5:459-477.
[7] Aoki N, Scofield G N, Wang X D, Offler H E, Patrick J W, Furbank R T . Pathway of sugar transport in germinating wheat seeds. Plant Physiol, 2006,141:1255-1263.
doi: 10.1104/pp.106.082719
[8] Hong Y F, Ho T H D, Wu C F, Ho S L, Yeh R H, Lu C A, Chen P W, Yu L C, Chao A, Yu A M . Convergent starvation signals and hormone crosstalk in regulating nutrient mobilization upon germination in cereals. Plant Cell, 2012,24:2857-2873.
doi: 10.1105/tpc.112.097741
[9] Ma Z, Marsolais F, Bernards M A, Sumarahb M W, Bykovad N V, Lgamberdiev A U . Glyoxylate cycle and metabolism of organic acids in the scutellum of barley seeds during germination. Plant Sci, 2016,248:37-44.
doi: 10.1016/j.plantsci.2016.04.007
[10] 鲜孟筑, 杨萍, 胡立勇, 徐正华 . 油菜种子萌发成苗期耐低温性评价. 作物杂志, 2015, ( 5):116-122.
Xian M Z, Yang P, Hu L Y, Xu Z H . Comprehensive evaluation of low temperature tolerance in rapeseed during germination and emergence periods. Crops, 2015, ( 5):116-122 (in Chinese with English abstract).
[11] Thompson K, Grime J P, Mason G . Seed germination in response to diurnal fluctuations of temperature. Nature, 1977,267:147-149.
[12] Upadhyaya H D, Wang Y H, Sastry D V S S R, Dwivedi S L, Prasad P V V, Burrell A M, Klein R R, Morris G P, Klein P E . Association mapping of germinability and seedling vigor in sorghum under controlled low-temperature conditions. Genome, 2016,59:137-145.
[13] Franks C D, Burow G B, Burke J J . A comparison of us and Chinese sorghum germplasm for early season cold tolerance. Crop Sci, 2006,46:1371-1376.
[14] 张丽霞, 张明政, 张灵敏, 霍岩, 王春语, 丛玲 . 高粱种子萌发期耐低温材料的筛选与鉴定. 西南农业学报, 2017,30:20-25.
Zhang L X, Zhang M Z, Zhang L M, Huo Y, Wang C Y, Cong L . Screening and identification of low-temperature resistant materials at seed stage. Southwest China J Agric Sci, 2017,30:20-25 (in Chinese with English abstract).
[15] 张海燕, 史红梅, 杨彬, 周忠宇, 张桂香 . 高粱种子耐冷材料筛选鉴定与利用评价. 种子, 2016,35(3):65-67.
Zhang H Y, Shi H M, Yang B, Zhou Z Y, Zhang G X . Utilization evaluation and screening identification on the chilling tolerance materials of sorghum seeds. Seed, 2016,35(3):65-67 (in Chinese with English abstract).
[16] 朱晨曦, 马艳青, 杨博智, 张竹青 . 不同辣椒品种种子萌发期耐低温性的研究. 中国蔬菜, 2015, ( 8):34-38.
Zhu C X, Ma Y Q, Yang B Z, Zhang Z Q . Studies on low temperature tolerance of different pepper varieties in seed germination stage. Chin Veg, 2015, ( 8):34-38 (in Chinese with English abstract).
[17] 王俊娟, 王德龙, 阴祖军, 王帅, 樊伟丽, 陆许可, 穆敏, 郭丽雪, 叶武威, 喻树迅 . 陆地棉萌发至幼苗期抗冷性的鉴定. 中国农业科学, 2016,49:3332-3346.
doi: 10.3864/j.issn.0578-1752.2016.17.008
Wang J J, Wang D L, Yin Z J, Wang S, Fan W L, Lu X K, Mu M, Guo L X, Ye W W, Yu S X . Identification of the chilling resistance from germination stage to seedling stage in upland cotton. Sci Agric Sin, 2016,49:3332-3346 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2016.17.008
[18] 张雪峰, 张立军, 胡滨 . 玉米萌发期种子耐低温鉴定指标的筛选. 辽宁农业科学, 2011, ( 1):25-30.
Zhang X F, Zhang L J, Hu B . Effects of chilling stress on chill-resistance physiological indexes of maize germinating seeds. Liaoning Agric Sci, 2011, ( 1):25-30 (in Chinese with English abstract).
[19] 高利英, 邓永胜, 韩宗福, 孔凡金, 申贵芳, 李汝忠, 尹燕枰 . 黄淮棉区棉花品种种子萌发期低温耐受性评价. 棉花学报, 2018,30:36-44.
Gao L Y, Deng Y S, Han Z F, Kong F J, Shen G F, Li R Z, Yin Y P . Evaluation of the low-temperature tolerance of cotton varieties in the Huang-Huai region during seed germination. Cotton Sci, 2018,30:36-44 (in Chinese with English abstract).
[20] Lu Q S, Dahlberg J A . Chinese sorghum genetic resources. Econ Bot, 2001,55:401-425.
doi: 10.1007/BF02866563
[21] 高士杰, 陈冰嬬, 李继洪, 贾俊英, 侯玉波 . 中国高粱春播早熟区雄性不育系存在的问题探讨. 吉林农业科学, 2012,37(5):9-11.
Gao S J, Chen B X, Li J H, Jia J Y, Hou Y B . Discussions on problems in male sterile line of sorghum in spring seeding early-maturing region in China. J Jilin Agric Sci, 2012,37(5):9-11 (in Chinese with English abstract).
[22] Balota M, Payne W A, Veergoni S K, Stewrt B A, Rosenow D T . Respiration and its relationship to germination, emergence, and early growth under cool temperatures in sorghum. Crop Sci, 2010,50:1414-1422.
[23] 常博文, 钟鹏, 刘杰, 唐中华, 高亚冰, 于洪久, 郭炜 . 低温胁迫和赤霉素对花生种子萌发和幼苗生理响应的影响. 作物学报, 2019,45:122-134.
Chang B W, Zhong P, Liu J, Tang Z H, Gao Y B, Yu H J, Guo W . Effect of low-temperature stress and gibberellin on seed germination and seedling physiological responses in peanut. Acta Agron Sin, 2019, 45:122-134 (in Chinese with English abstract).
[24] 张海艳 . 低温对鲜食玉米种子萌发及幼苗生长的影响. 植物生理学报, 2013,49:347-350.
Zhang H Y . Effects of low temperature on seed germination and seedling growth of fresh corn. Acta Phytophysiol Sin, 2013,49:347-350 (in Chinese with English abstract).
[25] 江玲, 侯名语, 刘世家, 陈亮明, 刘喜, 翟虎渠, 万建民 . 水稻种子低温萌发生理机制的初步研究. 中国农业科学, 2005,38:480-485.
Jiang L, Hou M Y, Liu S J, Chen L M, Liu X, Zhai H Q, Wan J M . Physiological mechanism of seed germination ( Oryza sativa L.) under low temperature. Sci Agric Sin, 2005,38:480-485 (in Chinese with English abstract).
[26] Masondo N A, Kulkarni M G, Finnie J F, Staden J V . Influence of biostimulants-seed-priming on, Ceratotheca triloba, germination and seedling growth under low temperatures, low osmotic potential and salinity stress. Ecotox Environ Safe, 2018,147:43-48.
[27] Sergio M C, Dante R T, Diodoro G S, Violeta C, Leopoldo M C, Hubert T . Phenotype, seed size and temperature affect the germinative capacity of Agave cupreata. Seed Sci Technol, 2017,45:1-6.
doi: 10.15258/sst.2017.45.1.05
[28] 耿广东, 程智慧, 张素勤 . 低温发芽鉴定茄子耐冷性的研究. 种子, 2006,25(6):43-46.
Geng G D, Cheng Z H, Zhang S Q . Appraisal of eggplant chilling-resistant by low temperature germination. Seed, 2006,25(6):43-46 (in Chinese with English abstract).
[29] 王钰静, 谢磊, 李志博, 魏亦农, 林海荣 . 低温胁迫对北疆棉花种子萌发的影响及其耐冷性差异评价. 种子, 2014,33(5):74-77.
Wang Y J, Xie L, Li Z B, Wei Y N, Lin H R . Effects of low temperature stress to germination of cotton seeds and evaluation of their cold resistance in northern Xinjiang. Seed, 2014,33(5):74-77 (in Chinese with English abstract).
[30] 徐建伟, 张晨, 曾晓燕, 张小均, 李志博, 魏亦农 . 近十年新疆北疆主栽棉花种子低温萌发能力差异评价. 新疆农业科学, 2017,54:1569-1578.
Xu J W, Zhang C, Zeng X Y, Zhang X J, Li Z B, Wei Y N . Evaluation of seed germination of main-cultivated cotton under low temperature in northern Xinjiang in recent ten years. Xinjiang Agric Sci, 2017,54:1569-1578 (in Chinese with English abstract).
[31] 王艺陶, 周宇飞, 李丰先, 依兵, 白薇, 闫彤, 许文娟, 高明超, 黄瑞冬 . 基于主成分和SOM聚类分析的高粱品种萌发期抗旱性鉴定与分类. 作物学报, 2014,40:110-121.
doi: 10.3724/SP.J.1006.2014.00110
Wang Y T, Zhou Y F, Li F X, Yi B, Bai W, Yan T, Xu W J, Gao M C, Huang R D . Identification and classification of Sorghum cultivars for drought resistance during germination stage based on principal components analysis and self organizing map cluster analysis. Acta Agron Sin, 2014,40:110-121 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2014.00110
[32] 龙卫华, 浦惠明, 张洁夫, 戚存扣, 张学昆 . 甘蓝型油菜发芽期的耐盐性筛选. 中国油料作物学报, 2013,35:271-275.
Long W H, Pu H M, Zhang J F, Qi C K, Zhang X K . Screening of Brassica napus for salinity tolerance at germination stage. Chin J Oil Crop Sci, 2013,35:271-275 (in Chinese with English abstract).
[33] Tiryaki I, Andrews D J . Germination and seedling cold tolerance in sorghum: I. Evaluation of rapid screening methods. Agron J, 2001,93:1386-1391.
[34] Yu J, Tuinstra M R, Claassen M M, Gordon W B, Witt M D . Analysis of cold tolerance in sorghum under controlled environment conditions. Field Crops Res, 2004,85:21-30.
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