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作物学报 ›› 2015, Vol. 41 ›› Issue (04): 658-665.doi: 10.3724/SP.J.1006.2015.00658

• 研究简报 • 上一篇    下一篇

利用SSR荧光标记构建20个高粱品种指纹图谱

王瑞1,张福耀1,*,程庆军1,田承华1,凌亮2   

  1. 1山西省农业科学院高粱研究所 / 高粱遗传与种质创新山西省重点实验室, 山西榆次030600;2山西省农业科学院食用菌研究所,山西太原 030031
  • 收稿日期:2014-10-08 修回日期:2015-02-06 出版日期:2015-04-12 网络出版日期:2015-03-02
  • 通讯作者: 张福耀, E-mail: zfy5607@163.com, Tel: 13835425607
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-06-01-01)和酿造专用高粱育种及利用山西省科技创新重点团队(2014131015)项目资助。

Establishment of 20 Sorghum Hybrids Fingerprints Using SSR Fluorescent Marker

WANG Rui1,ZHANG Fu-Yao1,*,CHENG Qing-Jun1,TIAN Cheng-Hua1,LING Liang2   

  1. 1Sorghum Institute, Shanxi Academy of Agricultural Sciences / Key Laboratory of Genetic and Germplasm Innovation in sorghum for Shanxi Province, Shanxi 030600, China; 2Institute of Edible Fungi, Shanxi Academy of Agricultural Sciences, Shanxi 030031, China
  • Received:2014-10-08 Revised:2015-02-06 Published:2015-04-12 Published online:2015-03-02
  • Contact: 张福耀, E-mail: zfy5607@163.com, Tel: 13835425607

摘要:

为建立高粱种子纯度及真实性鉴定技术体系, 构建高粱栽培品种DNA指纹图谱数据库是必要的。利用SSR荧光标记技术筛选出36对SSR荧光标记引物,构建我国高粱杂种优势利用以来中晚熟区主推的20个品种的SSR指纹图谱。36对SSR引物共扩增出235个等位基因,平均每个等位基因检测到多态性位点7个,多态性位点变化范围为2~12个。20个高粱品种36个SSR位点的遗传多样性指数和多态性信息量的变化范围分别为0.3490~0.8813和0.3144~0.8696,平均值分别为0.6976和0.6571。从36对SSR引物中筛选到多态性丰富的2对引物作为高粱品种鉴定的特征引物,2对SSR特征引物组合可区分所有供试品种。20个高粱品种的SSR指纹图谱互不相同,可以作为各品种特定的图谱,为品种鉴别提供依据。

关键词: 高粱, 品种鉴定, SSR荧光标记, 指纹图谱

Abstract:

To establish a standard technical system for purity and authenticity identification of sorghum seeds,it is necessary to construct a DNA fingerprinting database of major sorghum hybrids. The fluorescent marker detection of simple sequence repeat (SSR) was used to establish SSR fingerprints of 20 sorghum hybrids that are popularized in medium and late-maturing areas in China since heterosis has been utilized. Thirty-six pairs of SSR primers were screened and 235 alleles were amplified with the set of primers. The number of alleles per locus ranged from two to twelve with an average of seven. The range of gene diversity and polymorphism information content (PIC) at 36 SSR loci of 20 hybrids were 0.3490–0.8813 and 0.3144–0.8696, with an average of 0.6976 and 0.6571, respectively. Among 36 SSR primers, two primer pairs with high polymorphism were identified as the specific primers for distinguishing the all 20 sorghum hybrids, resulting in the fingerprints for the 20 sorghum hybrids. The presented data and technology provides an important basis for hybrids identification in sorghum.

Key words: Sorghum, Hybrid identification, SSR fluorescent marker, Fingerprint

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