作物学报 ›› 2019, Vol. 45 ›› Issue (11): 1664-1671.doi: 10.3724/SP.J.1006.2019.92003
刘江宁1,2,王楚鑫1,2,张宏根1,2,*(),缪一栩1,2,高海林1,2,许作鹏1,2,刘巧泉1,2,汤述翥1,2
LIU Jiang-Ning1,2,WANG Chu-Xin1,2,ZHANG Hong-GEN1,2,*(),MIAO Yi-Xu1,2,GAO Hai-Lin1,2,XU Zuo-Peng1,2,LIU Qiao-Quan1,2,TANG Shu-Zhu1,2
摘要:
发掘水稻黑条矮缩病的抗性基因有助于抗病品种的选育, 减少黑条矮缩病对水稻生产的危害。本研究构建了包含222个家系的L5494/IR36重组自交系群体。对该群体进行黑条矮缩病的田间诱发鉴定, 抗性亲本IR36发病率为28.70%, 感病亲本L5494发病率为84.26%, 群体发病率范围为11.21%~89.81%。利用134对分子标记构建覆盖12条染色体的遗传连锁图谱, 总遗传距离为1475.97 cM, 平均标记间距为11.1 cM。利用QTL IciMapping 4.0对抗黑条矮缩病QTL进行分析, 共检测到4个QTL, 其中第1、第2、第9染色体上QTL的表型贡献率分别为12.64%、16.00%和8.43%, 抗病等位基因来自抗病亲本IR36; 第6染色体上QTL的表型贡献率为10.82%, 抗病等位基因来自感病亲本L5494。在此基础上, 利用93-11为供体、日本晴为背景的近等基因系材料, 在qRBSDV-1定位区间内检测到来自93-11的抗性QTL。本研究结果为水稻黑条矮缩病抗性基因定位及分子标记辅助选择育种提供借鉴。
[1] | Milne R G, Lovisolo O . Maize rough dwarf and related viruses. Adv Virus Res, 1977,21:267-341. |
[2] | Azuhata F, Uyeda I, Kimura I, Shikata E . Close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses. J Gen Virol, 1993,74:1227-1232. |
[3] | Wang Z H, Fang S G, Xu J L, Sun L Y, Li D W, Yu J L . Sequence analysis of the complete genome of rice black-streaked dwarf virus isolated from maize with rough dwarf disease. Virus Genes, 2003,27:163-168. |
[4] | Zhang H M, Chen J P, Lei J L, Adama M J . Sequence analysis shows that a dwarfing disease on rice, wheat and maize in China is caused by rice black-streaked dwarf virus. Eur J Plant Pathol, 2001,107:563-567. |
[5] | 王朝辉, 周益军, 范永坚, 程兆榜, 张文荟 . 江苏水稻黑条矮缩病毒 S10 的cDNA克隆序列分析. 中国病毒学, 2002,17(2):142-144. |
Wang C H, Zhou Y J, Fan Y J, Cheng Z B, Zhang W H . cDNA clone and sequence analysis of segment 10 of rice black-streaked dwarf Fijivirus in Jiangsu. Virol Sin, 2002,17(2):142-144 (in Chinese with English abstract). | |
[6] | 李德葆, 王拱辰, 盛方镜 . 浙江省水稻病毒病的发生规律和防治. 植物病理学报, 1979,9:73-87. |
Li D B, Wang Z C, Sheng F J . Epidemological study on rice virus disease and their control in Zhejiang province. Acta Phytopathol Sin, 1979,9:73-87 (in Chinese with English abstract). | |
[7] | Wang H D, Chen J P, Wang A G, Jiang X H, Adama M J . Studies on the epidemiology and yield losses from rice black-streaked dwarf disease in a recent epidemic in Zhejiang province, China. Plant Pathol, 2009,58:815-825. |
[8] | 陈声祥, 吴惠玲, 廖璇刚, 吕永平, 沈升法, 王藕芳, 金梅松 . 水稻黑条矮缩病在浙中的回升流行原因分析. 浙江农业科学, 2000, ( 6):287-289. |
Chen S X, Wu H L, Liao X G, Lyu Y P, Shen S F, Wang O F, Jin M S . The prevalent reason of rice black-streaked dwarf viral disease in the middle of Zhejiang. Zhejiang Agric Sci, 2000, ( 6):287-289 (in Chinese with English abstract). | |
[9] | 孙枫, 徐秋芳, 程兆榜, 范永坚, 周益军 . 中国水稻黑条矮缩病研究进展. 江苏农业学报, 2013,29:195-201. |
Sun F, Xu Q F, Cheng Z B, Fan Y J, Zhou Y J . Advances in rice black-streaked dwarf disease in China. Jiangsu J Agric Sci, 2013,29:195-201 (in Chinese with English abstract). | |
[10] | 任应党, 鲁传涛, 王锡锋 . 水稻黑条矮缩病暴发流行原因分析——以河南开封为例. 植物保护, 2016,42(3):8-16. |
Ren Y D, Lu C T, Wang X F . Analysis of the reason for the outbreak epidemics of the rice black-streaked dwarf disease: Kaifeng in Henan Province as an example. Plant Prot, 2016,42(3):8-16 (in Chinese with English abstract). | |
[11] | 王宝祥, 江玲, 陈亮明, 卢百关, 王琦, 黎光泉, 樊继伟, 程遐年, 翟虎渠, 徐大勇, 万建民 . 水稻黑条矮缩病抗性资源的筛选和抗性QTL的定位. 作物学报, 2010,36:1258-1264. |
Wang B X, Jiang L, Chen L M, Lu B G, Wang Q, Li G Q, Fan J W, Cheng X N, Zhai H Q, Xu D Y, Wan J M . Screening of rice resources against rice black-streaked dwarf virus and mapping of resistant QTL. Acta Agron Sin, 2010,36:1258-1264 (in Chinese with English abstract). | |
[12] | 李爱宏, 戴正元, 季红娟, 张小祥, 李育红, 潘存红, 张洪熙, 潘学彪 . 不同基因型水稻种质对黑条矮缩病抗性的初步分析. 扬州大学学报(农业与生命科学版), 2008,29(3):18-22. |
Li A H, Dai Z Y, Ji H J, Zhang X X, Li Y H, Pan C H, Zhang H X, Pan X B . Preliminary analysis on resistance of rice black-streaked dwarf viral visease for germplasms with different gene-types. J Yangzhou Univ(Agric & Life Sci Edn), 2008,29(3):18-22 (in Chinese with English abstract). | |
[13] | 李爱宏, 潘存红, 戴正元, 肖宁, 余玲, 李育红, 张小祥, 张洪熙, 潘学彪 . 以标记辅助选择改良江苏主栽粳稻品种“淮稻5号”黑条矮缩病抗性. 作物学报, 2012,38:1775-1781. |
Li A H, Pan C H, Dai Z Y, Xiao N, Yu L, Li Y H, Zhang X X, Zhang H X, Pan X B . Using marker-assisted selection to improve the resistance to rice black-streaked dwarf viral disease of Huaidao 5, an elite japonica rice cultivar in Jiangsu. Acta Agron Sin, 2012,38:1775-1781 (in Chinese with English abstract). | |
[14] | 王宝祥, 胡金龙, 孙志广, 宋兆强, 卢百关, 周振玲, 樊继伟, 秦德荣, 刘裕强, 江铃, 徐大勇, 万建民 . 水稻黑条矮缩病抗性评价方法及抗性资源筛选. 作物学报, 2014,40:1521-1530. |
Wang B X, Hu J L, Sun Z G, Song Z Q, Lu B G, Zhou Z L, Fan J W, Qin D R, Liu Y Q, Jiang L, Xu D Y, Wan J M . An evaluation system for rice black-streaked dwarf virus disease and screening for resistant rice germplasm. Acta Agron Sin, 2014,40:1521-1530 (in Chinese with English abstract). | |
[15] | 潘存红, 李爱宏, 陈宗祥, 吴林波, 戴正元, 张洪熙, 黄年生, 陈夕军, 张亚芳, 左示敏, 潘学彪 . 水稻黑条矮缩病抗性QTL分析. 作物学报, 2009,35:2213-2217. |
Pan C H, Li A H, Chen Z X, Wu L B, Dai Z Y, Zhang H X, Huang N S, Chen X J, Zhang Y F, Zuo S M, Pan X B . Detection of QTL for resistance to rice black-streaked dwarf viral disease. Acta Agron Sin, 2009,35:2213-2217 (in Chinese with English abstract). | |
[16] | Li A H, Pan C H, Wu L B, Dai Z Y, Zuo S M, Xiao N, Yu L, Li Y H, Zhang X X, Xue W X, Zhang H X, Pan X B . Identification and fine mapping of qRBSDV-6MH, a major QTL for resistance to rice black-streaked dwarf virus disease. Mol Breed, 2013,32:1-13. |
[17] | Sun Z G, Liu Y Q, Xiao S Z, Hu J L, Pan G, He J, Xu T T, Huang J, Qiu Z Y, Fan D J, Zhang L, Liu L L, Jiang L, Cheng X N, Zhai H Q, Wan J M . Identification of quantitative trait loci for resistance to rice black-streaked dwarf virus disease and small brown planthopper in rice. Mol Breed, 2017,37:72. |
[18] | Zhou T, Du L L, Wang L J, Wang Y, Gao C Y, Lan Y, Sun F, Fan Y J, Wang G L, Zhou Y J . Genetic analysis and molecular mapping of QTLs for resistance to rice black-streaked dwarf disease in rice. Sci Rep, 2015,5:10509. |
[19] | Zhang H G, Ge Y S, Wang M Y, Liu J N, Si H, Zhang L J, Liang G H, Gu M H, Tang S Z . Mapping QTLs conferring resistance to rice black-streaked dwarf disease in rice (Oryza sativa L.). Euphytica, 2016,212:323-330. |
[20] | 王英 . 水稻对黑条矮缩病的抗性遗传分析及基因定位. 南京农业大学硕士学位论文,江苏南京, 2011. |
Wang Y . Genetic Analysis and Molecular Mapping of QTL for Rice Black Streaked-dwarf Disease Resistance in Rice. MS Thesis of Nanjing Agricultural University, Nanjing, Jiangsu, China, 2011 (in Chinese with English abstract). | |
[21] | Zheng T Q, Yang J, Zhong W G, Zhai H Q, Zhu L H, Fan F J, Juahar A, Yang J H, Wang J, Zhu J Y, Veronica U, Xu J L, Li Z K . Novel loci for field resistance to black-streaked dwarf and stripe viruses identified in a set of reciprocal introgression lines of rice (Oryza sativa L.). Mol Breed, 2012,29:925-938. |
[22] | Xu Z P, Li S C, Zhang C Q, Zhang B C, Zhu K Z, Zhou Y H, Liu Q Q . Genetic connection between cell-wall composition and grain yield via parallel QTL analysis in indica and japonica subspecies. Sci Rep, 2017,7:12561. |
[23] | Zhu K Z, Zhang H, Liu X Y, Hu B, Zhang C Q, Gu M H, Liu Q Q . Development and high-throughput genotyping of substitution lines carrying the chromosome segments of indica 9311 in the background of japonica Nipponbare. J Genet Genomics, 2011,38:603-611. |
[24] |
周彤, 吴丽娟, 王英, 程兆榜, 季英华, 范永坚, 周益军 . 水稻对黑条矮缩病感病生育期研究初报. 华北农学报, 2010,25(6):128-131.
doi: 10.7668/hbnxb.2010.06.024 |
Zhou T, Wu L J, Wang Y, Cheng Z B, Ji Y H, Fan Y J, Zhou Y J . Preliminary report on the susceptible growth stages of rice to rice black-streaked dwarf disease. Acta Agric Boreali Sin, 2010,25(6):128-131 (in Chinese with English abstract).
doi: 10.7668/hbnxb.2010.06.024 |
|
[25] | Rogers S O, Bendich A J. Extraction of DNA from plant tissues. In: Gelvin S B, Schilperoort R A, Verma D P S, eds. Plant Molecular Biology Manual. Dordrecht: Springer, 1989. pp 73-83. |
[26] | Meng L, Li H H, Zhang L Y, Wang J K . QTL IciMapping: integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop J, 2015,3:269-283. |
[27] | McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T . Report on QTL nomenclature. Rice Genet Newsl, 1997,14:11-13. |
[28] | 周彤, 王英, 吴丽娟, 范永坚, 周益军 . 水稻品种抗黑条矮缩病人工接种鉴定方法. 植物保护学报, 2011,38:301-305. |
Zhou T, Wang Y, Wu L J, Fan Y J, Zhou Y J . Method of artificial inoculation identification of rice cultivar resistance to rice black-streaked dwarf. Acta Phytophy Sin, 2011,38:301-305 (in Chinese with English abstract). | |
[29] | 王宝祥, 谭维娜, 卢百关, 宋兆强, 徐大勇 . 水稻品种‘连粳7号’对黑条矮缩病的抗性分析. 中国农学通报, 2014,30(12):51-56. |
Wang B X, Tan W N, Lu B G, Song Z Q, Xu D Y . Resistance analysis of the rice cultivar ‘Lianjing 7’ to rice black-streaked dwarf disease. Chin Agric Sci Bull, 2014,30(12):51-56 (in Chinese with English abstract). | |
[30] | Wang Q, Liu Y Q, Hu J L, Zhang Y X, Xie K, Wang B X, Le Quang T, Song Z Q, Wu H, Liu Y L, Jiang L, Liu S J, Cheng X N, Wang C M, Zhai H Q, Wan J M . Detection of quantitative trait loci (QTLs) for resistances to small brown planthopper and rice stripe virus in rice using recombinant inbred lines. Int J Mol Sci, 2013,14:8406-8421. |
[31] | Wang B X, Wen Y B, Liu Y, Wang K J, Dai H M, Liu J B, Pu H C, Xu D Y . Correlation analysis of rice resistance against black-streaked dwarf virus disease (RBSDV) and small brown planthopper (Laodelphax striatellus Fallen). Plant Diseases Pests, 2016,7(3):1-5. |
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