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作物学报 ›› 2020, Vol. 46 ›› Issue (5): 661-667.doi: 10.3724/SP.J.1006.2020.94119

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用RIL群体创制低山嵛酸花生新种质

郭建斌,黄莉,刘念,罗怀勇,周小静,陈伟刚,吴贝,淮东欣,任小平,姜慧芳()   

  1. 中国农业科学院油料作物研究所 / 农业农村部油料作物生物学与遗传育种重点实验室, 湖北武汉 430062
  • 收稿日期:2019-08-14 接受日期:2019-12-26 出版日期:2020-05-12 网络出版日期:2020-01-16
  • 通讯作者: 姜慧芳
  • 作者简介:E-mail:guojianbin1990@163.com
  • 基金资助:
    本研究由国家自然科学基金项目(31571713);本研究由国家自然科学基金项目(31761143005);本研究由国家自然科学基金项目(31801403);本研究由国家自然科学基金项目(31871666);农作物种质资源保护项目(2017NWB033);国家农作物种质资源共享服务平台(NICGR2017-36);国家现代农业产业技术体系建设专项(CARS-13-花生种质资源评价)资助

Novel peanut genotype with low behenic acid developed from recombinant inbred lines

Jian-Bin GUO,Li HUANG,Nian LIU,Huai-Yong LUO,Xiao-Jing ZHOU,Wei-Gang CHEN,Bei WU,Dong-Xin HUAI,Xiao-Ping REN,Hui-Fang JIANG()   

  1. Oil Crops Research Institute, China Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, the Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
  • Received:2019-08-14 Accepted:2019-12-26 Published:2020-05-12 Published online:2020-01-16
  • Contact: Hui-Fang JIANG
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31571713);This study was supported by the National Natural Science Foundation of China(31761143005);This study was supported by the National Natural Science Foundation of China(31801403);This study was supported by the National Natural Science Foundation of China(31871666);the Crop Germplasm Resources Protection Project(2017NWB033);the Plant Germplasm Resources Sharing Platform(NICGR2017-36);the China Agricultural Research System (CARS-13-Germplasm Resource Evaluation for Peanut)

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摘要：

花生是我国食用植物油的重要来源, 改善花生油脂品质是我国花生育种的重要目标。利用不同遗传背景的亲本(中花10号×ICG12625)杂交构建含有140个家系的重组自交系群体(RIL), 对超长链饱和脂肪酸(花生酸、山嵛酸和二十四碳烷酸)含量测定表明, RIL家系超长链饱和脂肪酸含量的变异范围为4.27%~7.05%, 均值为5.54%, 最低值分别比父本和母本低1.46和1.63个百分点, 降低率达25.27%和27.62%; 山嵛酸含量的变异范围为1.86%~3.37%, 均值为2.53%, 3份家系的山嵛酸含量稳定低于2% (QT0002、QT0075和QT0120), 比低值亲本中花10号分别低0.57、0.51和0.51个百分点, 降低率分别为23.45%、20.98%和20.98%。利用前期构建的遗传图谱, 通过WinQTLcart软件检测到一个与山嵛酸相关的稳定的QTL, 位于B04染色体13.31~16.34 M内, 该区间内含有131个预测基因。本结果为高油酸花生品种的山嵛酸遗传改良奠定了基础。

关键词: 花生, 重组自交系, 脂肪酸, 山嵛酸

Abstract:

Peanut is an important source of edible vegetable oil in China, and improving the quality of peanut oil is an important goal in peanut breeding. In this study, the contents of very long-chain saturated fatty acids (arachidic acid, behenic acid, and tetracosanoic acid) were determined by using RIL population containing 140 lines derived from the cross between Zhonghua 10 and ICG12625 with different genetic backgrounds. The content of very long- chain saturated fatty acid in the RILs was 4.27%-7.05%, with an average of 5.54%, the minimum value was 1.46 and 1.63 percentage points lower than that of male and female parents, with reduction rates of 25.27% and 27.62%, respectively. The behenic acid content ranged from 1.86% to 3.37%, with an average of 2.53%. Three lines of QT0002, QT0075, and QT0120 had behenic acid content lower than 2%, which was 0.57, 0.51, and 0.51 percentage points lower than that of Zhonghua10 with the reduction rate of 23.45%, 20.98%, and 20.98%, respectively. A stable QTL located in 13.31-16.34 M of B04 was detected using a linkage map and WinQTLcart software. In this region of 13.31-16.34 M contained 131 prediction genes. The results lay a foundation for the genetic improvement of low behenic acid and high-oleic peanut varieties.

Key words: peanut, recombined inbreed lines (RILs), fatty acid, behenic acid

图1

亲本在超长链饱和脂肪酸上的差异"

图2

超长链饱和脂肪酸相关性分析"

表1

亲本及RIL群体中山嵛酸表型统计分析"

年份
Year		 亲本 Parent (%) RIL群体 RIL population (%)
中花10号
Zhonghua 10		 ICG12625 最小值
Min		 最大值
Max		 均值
Mean		 标准差
SD		 变异系数
CV		 偏度
Skewness		 峰度
Kurtosis
2015 2.17 2.70 1.80 3.51 2.53 0.31 12.39 0.44 0.34
2016 2.58 3.23 1.90 3.69 2.66 0.37 13.96 0.48 0
2017 2.52 2.81 1.68 3.21 2.40 0.29 11.99 0.08 -0.12

表2

3份低山嵛酸家系其他脂肪酸表现"

家系
Line		 山嵛酸
Behenic acid		 棕榈酸
Palmitic acid		 硬脂酸
Stearic acid		 油酸
Oleic acid		 亚油酸
Linoleic acid		 花生酸
Arachidic acid		 花生烯酸
Arachidonic acid		 二十四碳烷酸
Tetracosanoic acid		 超长链饱和脂肪酸
Very long-chain
saturated fatty acid
QT0002 1.86 11.86 2.93 48.49 31.67 1.32 0.88 1.09 4.27
QT0075 1.92 12.73 3.51 42.92 35.78 1.41 0.66 1.13 4.46
QT0120 1.92 11.83 3.69 50.47 28.76 1.54 0.69 1.20 4.65
Zhonghua 10 2.43 10.45 5.30 48.54 29.29 2.09 0.65 1.39 5.90
ICG12625 2.91 13.48 2.48 40.02 37.29 1.32 1.13 1.49 5.73

图3

RIL群体山嵛酸性状在3年中的分布"

表3

标记GM2246-2和AGGS1236在RIL群体中山嵛酸含量的表型效应"

标记
Marker		 基因型
Genotype		 山嵛酸含量Content of behenic acid (%)
2015 2016 2017 均值 Mean
GM2246-2 AA 2.43±0.25 2.57±0.34 2.30±0.25 2.44±0.23
aa 2.67±0.35 2.81±0.39 2.52±0.31 2.66±0.31
aa-AA 0.24** 0.24** 0.22** 0.22**
AGGS1236 AA 2.42±0.28 2.58±0.67 2.31±0.45 2.45±0.25
aa 2.70±0.32 2.78±0.75 2.52±0.31 2.66±0.29
aa-AA 0.28** 0.20** 0.21** 0.21**

图4

标记在RIL群体中超长链饱和脂肪酸的表型效应"

图5

GO富集分析"

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