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作物学报 ›› 2020, Vol. 46 ›› Issue (01): 40-51.doi: 10.3724/SP.J.1006.2019.94066

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

陆地棉钾转运体基因GhHAK5启动子的克隆与功能分析

晁毛妮1,胡海燕1,*(),王润豪1,陈煜2,付丽娜1,刘庆庆1,王清连1   

  1. 1 河南科技学院/现代生物育种河南省协同创新中心, 河南新乡 453003
    2 山东棉花研究中心, 山东济南 250100
  • 收稿日期:2019-04-25 接受日期:2019-08-09 出版日期:2020-01-12 网络出版日期:2020-03-04
  • 通讯作者: 胡海燕
  • 作者简介:E-mail: chaomaoni@126.com
  • 基金资助:
    本研究由国家自然科学基金项目(31601347);河南省博士后科学基金项目(1902042);河南省科技攻关计划项目(192102110030);河南省高等学校重点科研计划项目资助(19A210013)

Cloning and functional analysis of promoter of potassium transporter gene GhHAK5 in upland cotton (Gossypium hirsutum L.)

Mao-Ni CHAO1,Hai-Yan HU1,*(),Run-Hao WANG1,Yu CHEN2,Li-Na FU1,Qing-Qing LIU1,Qing-Lian WANG1   

  1. 1 Henan Institute of Science and Technology/Henan Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, Henan, China
    2 Cotton Research Center of Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China
  • Received:2019-04-25 Accepted:2019-08-09 Published:2020-01-12 Published online:2020-03-04
  • Contact: Hai-Yan HU
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31601347);Henan Postdoctoral Science Foundation(1902042);Henan Scientific and Technological Research Program(192102110030);Key Research Projects of Henan Higher Education Institutions(19A210013)

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摘要：

KUP/HAK/KT钾转运体基因的转录调控是植物响应低钾胁迫的一项重要机制。克隆和分析棉花钾转运体基因的启动子, 不仅有助于了解其表达模式及调控机制, 对于改良棉花的钾吸收特性也具有重要意义。陆地棉钾转运体基因GhHAK5是一个在根中特异性高表达的基因, 其表达受低钾胁迫诱导, 目前关于该基因启动子的功能还不清楚。本研究以陆地棉品种百棉1号为材料, 通过PCR方法对GhHAK5上游2000 bp启动子片段(pGhHAK5)进行克隆, 并通过转化拟南芥、GUS组织定位和低钾诱导表达特性分析来研究其功能。结果表明, pGhHAK5除具有TATA-box和CAAT-box等基本顺式作用元件外, 还含有多个响应于光、逆境胁迫、植物激素和生物钟等的顺式作用元件。pGhHAK5与雷蒙德氏棉pGrHAK5在重要调控元件的数量和位置分布上具有较高的一致性, 均具有5个参与根特异性表达调控的元件(ATAAAAT)和1个参与低钾条件下转录调控的ARF转录因子结合位点(TGTCNN)。GUS组织化学染色结果显示, 转基因拟南芥幼苗的叶脉和胚轴维管束组织染色较深, 根系染色较浅; 成熟期转基因拟南芥植株的根、叶脉和花萼维管束组织染色较深, 茎和荚皮染色较浅, 表明pGhHAK5驱动的GUS主要在拟南芥成熟的根和地上部维管束组织中表达。进一步低钾诱导表达特性分析表明, PGhHAK5驱动的GUS在拟南芥幼苗幼嫩根中的表达很弱, 且其表达不受低钾胁迫诱导而增强, 表明PGhHAK5可能是一个主要在成熟根中具有功能的低钾诱导型启动子。转录组分析和荧光定量PCR结果表明, GhHAK5主要在成熟的根中表达, 且其表达受发育时期的影响, 该结果与pGhHAK5驱动的GUS在拟南芥根中的表达结果一致。本研究结果有助于深入了解GhHAK5表达调控的分子机制, 并为棉花钾吸收效率的提高及钾高效棉花品种的培育提供理论依据。

关键词: 启动子, 维管束组织, 钾转运体, 低钾, 陆地棉

Abstract:

Transcriptional regulation of KUP/HAK/KT potassium transporter gene is an important mechanism of plant response to low potassium stress. Cloning and analysis of promoter of potassium transporter gene in cotton is not only helpful to understand its expression pattern and regulation mechanism, but also important to improve the potassium absorption in cotton. Potassium transporter gene GhHAK5 is a highly expressed in roots and induced by low potassium stress in upland cotton, but the function of its promoter is still unclear. In this study, the 2000 bp promoter fragment of GhHAK5 was cloned from upland cotton variety Baimian 1 by using PCR amplification, and its function was analyzed by GUS histochemical staining and induced expression analysis of GUS under low potassium in pGhHAK5 transgenic Arabidopsis thaliana. In addition to TATA-box, CAAT-box and other basic cis-acting elements, pGhHAK5 also contained a number of cis-acting elements responsive to light, stress, phytohormone and circadian. pGhHAK5 was highly consistent with pGrHAK5 in the number and location of important regulatory elements, and had five root-specific expression regulatory elements (ATAAAAT) and an ARF transcription factor binding site (TGTCNN) involved in transcription regulation under low potassium conditions. GUS histochemical staining of transgenic Arabidopsis thaliana seedlings showed that the leaf veins and vascular tissue of hypocotyl were deeply stained, and the roots were shallowly stained. For mature Arabidopsis thaliana plants, enhanced GUS staining was observed in roots, leaf veins and the vascular tissue of calyx, and weakened GUS staining was observed in stem and pod skin, suggesting that pGhHAK5-driven GUS was mainly expressed in mature roots and vascular tissue of shoots. Induced expression analysis of GUS under low potassium in pGhHAK5 transgenic Arabidopsis thaliana showed that the expression of GUS driven by pGhHAK5 was weak in young roots of Arabidopsis thaliana seedlings, and its expression was not enhanced by low potassium stress. These results suggest that pGhHAK5 might be a potassium-deficient inducible promoter mainly in mature roots. Transcriptome and quantitative real-time PCR analysis showed that GhHAK5 expression in roots was affected by developmental stages, which was consistent with the results of GUS expression driven by pGhHAK5 in Arabidopsis thaliana. These results are helpful to understand the molecular mechanism of GhHAK5 expression regulation, and provide theoretical basis for improving potassium uptake efficiency and breeding potassium efficient varieties in cotton.

Key words: promoter, vascular tissues, potassium transporter, low potassium, upland cotton

图1

陆地棉GhHAK5启动子序列的扩增 1: GhHAK5启动子PCR扩增产物; 2: DNA分子量标准(DL2000)。"

表1

GhHAK5启动子区包含的顺式作用元件"

元件类型
Element type		 名称
Name		 拷贝数
Copy number		 基序
Motif sequence		 功能
Function
基础元件
Basal
element		 5UTR Py-rich stretch 1 TTTCTTCTCT 高转录水平顺式作用元件
cis-acting element conferring high transcription levels
TATA-box 61 TATA/ATATAT/TTTTA 转录起始位点-30核心启动子元件 Core promoter element around -30 of transcription start
CAAT-box 18 CAATT/CAAT/CCAAT 启动子和增强子区的一般顺式作用元件 Common cis-acting element in promoter and enhancer regions
光
Light		 Box I 1 TTTCAAA 光响应元件 Light responsive element
I-box 1 ATGATATGA 部分光响应元件 Part of a light responsive element
G-Box 1 CACGTT 光响应顺式作用调控元件 cis-acting regulatory element involved in light responsiveness
AT1-motif 1 ATTAATTTTACA 部分光响应模块 Part of a light responsive module
Box II 1 GTGGATATTATAT 部分光响应元件 Part of a light responsive element
生物钟
Circadian		 Circadian 1 CAANNNNATC 昼夜节律顺式作用调控元件 cis-acting regulatory element involved in circadian control
植物激素
Phytohormone		 CGTCA-motif 1 CGTCA 茉莉酸响应顺式作用元件 cis-acting regulatory element involved in the MeJA-responsiveness
TCA-element 1 GAGAAGAATA 水杨酸响应顺式作用元件 cis-acting element
involved in salicylic acid responsiveness
TGA-element 1 AACGAC 生长素响应元件 Auxin-responsive element
ERE 1 ATTTCAAA 乙烯响应元件 Ethylene-responsive element
逆境胁迫
Stress		 Box-W1 1 TTGACC 真菌诱导子响应元件
Fungal elicitor responsive element
HSE 4 AAAAAATTTC 热胁迫响应顺式作用元件 cis-acting element
involved in heat stress responsiveness
其他
Other		 Skn-1_motif 4 GTCAT 胚乳表达相关顺式调控元件 cis-acting regulatory element required for endosperm expression
AT-rich element 1 ATAGAAATCAA AT-rich DNA结合蛋白的结合位点(ATBP-1)
Binding site of AT-rich DNA binding protein
Root-specific motif 5 ATAAAAT 根特异性表达响应元件
Root-specific responsive element

图2

GhHAK5启动子的序列分析 核苷酸的+1位置指的是起始密码子ATG中的A的位置, ATG用红色表示。部分预测的顺式作用元件在图中用灰色阴影表示。1个ARF转录因子结合位点(TGTCNN)在图中用红色加下画线表示。5个根特异性表达元件(ATAAAAT)在图中用红色加粗表示。"

图3

GhHAK5同源基因启动序列的比较分析 加粗的为棉属4个种钾转运体基因HAK5的启动子。"

图4

T1代转基因拟南芥植株的PCR检测 M: marker; 1: 野生型拟南芥(阴性对照); 2: 表达载体pCAMBIA1381Z-pGhHAK5质粒(阳性对照); 3~8转基因拟南芥。"

图5

转pGhHAK5拟南芥植株的GUS组织化学染色 A: 野生型拟南芥; B: 转pGhHAK5拟南芥; 1~4: 拟南芥幼苗(1)及其叶片(2)、胚轴(3)和根(4)对应位置放大图; 5~12: 成熟期拟南芥植株的叶片(5)、茎(6)、花(7)、花对应位置放大图(8)、荚(9)、荚对应位置放大图(10)、根(11)和根对应位置放大图(12)。"

图6

转pGhHAK5拟南芥幼苗对低钾胁迫的响应 WT: 野生型拟南芥; HK: 正常钾; LK: 低钾。a: 拟南芥幼苗; b: 叶对应位置放大图; c: 根对应位置放大图。"

图7

陆地棉GhHAK5基因的时空表达特性分析 A: 陆地棉GhHAK5基因时空表达特性的转录组分析; B: 陆地棉GhHAK5基因时空表达特性的荧光定量PCR分析; C: 不同发育时期棉花根系形态的比较; **表示在0.01水平上差异显著。"

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