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作物学报 ›› 2020, Vol. 46 ›› Issue (01): 52-61.doi: 10.3724/SP.J.1006.2020.91024

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大麦重组自交系群体籽粒总花色苷含量和千粒重QTL定位

杨晓梦1,2,李霞1,2,普晓英1,2,杜娟1,2,Muhammad Kazim Ali3,杨加珍1,2,曾亚文1,2,*(),杨涛1,2   

  1. 1 云南省农业科学院生物技术与种质资源研究所, 云南昆明 650205
    2 云南省农业生物技术重点实验室, 云南昆明 650205
    3 卡拉奇乌尔都艺术与科技大学生物技术系, 卡拉奇 75300, 巴基斯坦
  • 收稿日期:2019-03-18 接受日期:2019-08-09 出版日期:2020-01-12 网络出版日期:2019-09-18
  • 通讯作者: 曾亚文
  • 作者简介:杨晓梦, E-mail: yxm89ccf@126.com
  • 基金资助:
    本研究由云南省应用基础研究计划项目(2017FD021);国家现代农业产业技术体系建设专项资助(CARS-05-01A-04)

QTL mapping for total grain anthocyanin content and 1000-kernel weight in barley recombinant inbred lines population

YANG Xiao-Meng1,2,LI Xia1,2,PU Xiao-Ying1,2,DU Juan1,2,Muhammad Kazim Ali3,YANG Jia-Zhen1,2,ZENG Ya-Wen1,2,*(),YANG Tao1,2   

  1. 1 Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China
    2 Agricultural Biotechnology Key Laboratory of Yunnan Province, Kunming 650205, Yunnan, China
    3 Department of Biotechnology, Fedural Urdu University of Arts, Science and Technology, Karachi 75300, Pakistan
  • Received:2019-03-18 Accepted:2019-08-09 Published:2020-01-12 Published online:2019-09-18
  • Contact: Ya-Wen ZENG
  • Supported by:
    This study was supported by Yunnan Applied Basic Research Projects of China(2017FD021);China Agriculture Research System(CARS-05-01A-04)

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摘要：

以云南特有的紫色大麦紫光芒裸二棱和澳大利亚引进的黄色大麦Schooner构建的193个重组自交系为材料, 对 2013—2015年3年3个试验点的大麦籽粒总花色苷含量和千粒重进行相关性分析和QTL定位。大麦总花色苷含量和千粒重之间呈显著或极显著负相关。共检测到12个总花色苷含量QTL, 分别位于1H、2H、4H、6H和7H染色体, 贡献率为5.06%~23.86%; 8个千粒重QTL, 分别位于2H、4H和7H染色体, 贡献率为4.67%~42.32%。贡献率大于10%的QTL有10个, 大于20%的有5个, 最大的可达42.32%。其中至少2年2点重复检测到2个总花色苷含量QTL, 分别位于2H Bmag0125-GBM1309和7H EBmatc0016-Bmag0206区间, 可分别解释表型变异的13.66%~17.76%和13.07%~16.43%; 3年3点重复检测到2个千粒重QTL, 分别位于2H Scssr03381-scssr07759和7H GBM1297-GBM1303区间, 可分别解释表型变异的4.67%~14.55%和34.51%~42.32%, 其加性作用方向均一致。控制总花色苷含量与千粒重的主效QTL同位于2H和7H染色体。

关键词: 大麦重组自交系, 总花色苷含量, 千粒重, 相关性, QTL定位

Abstract:

In this study, 193 recombinant inbred lines (RILs) derived from a cross between Ziguangmangluoerleng (purple barley, endemic to Yunnan, China) and Schooner (yellow barley introduced from Australia), grown at three sites of Yunnan province of China for three consecutive years (2013-2015), were used to determine total grain anthocyanin content, 1000-kernel weight and their correlations, and mapped QTLs. There was a significant negative correlation between total grain anthocyanin content and 1000-kernel weight. Twelve QTLs for total grain anthocyanin content were located on chromosomes 1H, 2H, 4H, 6H, and 7H which showed 5.06% to 23.86% of phenotypic variation. Eight QTLs for 1000-kernel weight were located on chromosomes 2H, 4H, and 7H, which explained phenotypic variation from 4.67% to 42.32%. Ten QTLs had phenotypic variation ≥10% and five QTLs ≥ 20%, while the highest phenotypic variation was 42.32%. In addition, two QTLs for total grain anthocyanin content were repeatedly detected at two sites for two years, and located on 2H Bmag0125 to GBM1309 and 7H EBmatc0016 to Bmag0206 interval, respectively, which accounted for 13.66% to 17.76%, and 13.07% to 16.43% of the phenotypic variation, respectively. Two QTLs for 1000-kernel weight were repeatedly detected at three sites for three years and located on 2H scssr03381 to scssr07759 and 7H GBM1297 to GBM1303 interval, which showed 4.67% to 14.55% and 34.51% to 42.32% of phenotypic variations, respectively, and their contribution rates of additive effects were consistent. The common major QTLs for two agronomic attributes were mainly distributed on chromosomes 2H and 7H. These results provide a basis for further fine mapping, cloning and marker-assisted breeding of beneficial genes related to total grain anthocyanin content and 1000-kernel weight.

Key words: barley RIL, total anthocyanin content, 1000-kernel weight, correlation, QTL mapping

表1

大麦籽粒总花色苷和千粒重表型值"

性状
Trait		 地点和年份
Site and year		 亲本Parents RIL群体 RIL population
紫光芒裸二棱
ZGMLEL		 Schooner 平均值
Mean		 标准差
SD		 变幅
Range		 变异系数
CV (%)		 偏度
Skewness		 峰度
Kurtosis
总花色苷含量
TAC (mg g-1)		 玉溪 Yuxi, 2013 0.96 0.48 0.65 C 0.22 0.36-1.38 33.85 1.22 0.75
白邑 Baiyi, 2014 1.03 0.63 0.79 B 0.21 0.50-1.50 26.58 1.33 1.09
嵩明 Songming, 2015 1.14 0.70 0.84 A 0.21 0.50-1.58 25.00 1.14 0.81
千粒重
TKW (g)		 玉溪 Yuxi, 2013 35.50 46.00 45.55 C 6.01 31.65-58.00 13.19 -0.34 -0.62
白邑 Baiyi, 2014 33.35 42.55 44.00 B 6.64 23.05-60.50 15.09 -0.28 -0.35
嵩明 Songming, 2015 31.00 40.05 42.36 A 7.53 25.00-57.60 17.78 -1.10 4.00

图1

大麦籽粒总花色苷含量和千粒重的次数分布 横坐标分别代表3年3个试验点(玉溪2013、白邑2014和嵩明2015年)大麦RIL群体总花色苷含量和千粒重; 纵坐标分别代表大麦RIL群体总花色苷含量和千粒重的株系数。"

表2

大麦籽粒总花色苷含量和千粒重的相关系数"

性状
Trait		 地点和年份
Site and year		 总花色苷含量 Total grain anthocyonin content 千粒重 1000-kernel weight
玉溪
Yuxi 2013		 白邑
Baiyi 2014		 嵩明
Songming 2015		 玉溪
Yuxi 2013		 白邑
Baiyi 2014		 嵩明
Songming 2015
总花色苷含量
TAC		 玉溪Yuxi 2013 1.00
白邑Baiyi 2014 0.918** 1.00
嵩明Songming 2015 0.873** 0.833** 1.00
千粒重
TKW		 玉溪Yuxi 2013 -0.223** -0.151* -0.233** 1.00
白邑Baiyi 2014 -0.163* -0.130 -0.174* 0.832** 1.00
嵩明Songming 2015 -0.146* -0.064 -0.173* 0.775** 0.720** 1.00

表3

大麦籽粒总花色苷含量和千粒重的QTL分析"

地点和年份
Site and year		 性状
Trait		 QTL 连锁群
Chr.		 标记区间
Marker interval		 LOD值
LOD value		 贡献率
Contribution (%)		 加性效应
Additive
玉溪 Yuxi
2013		 总花色苷
TAC		 qTAC-1H 1H GBM1234-Bmag0504 4.04 5.91 0.055
qTAC-2H 2H Bmag0125-GBM1309 10.42 13.66 0.080
qTAC-4H 4H HVBAMMGB84-BMAG0808 5.21 6.23 -0.054
qTAC-6H 6H GBMS0072-GBM1270 4.06 7.68 0.061
qTAC-7H 7H EBmatc0016-Bmag0206 9.82 13.07 -0.079
千粒重
TKW		 qTKW-2H 2H Scssr03381-scssr07759 7.54 14.55 -2.293
qTKW-4H 4H Scssr20569-Bmag0781 3.81 4.73 1.396
qTKW-7H 7H GBM1297-GMS056 26.14 42.32 -4.390
白邑 Baiyi
2014		 总花色苷
TAC		 qTAC-2H 2H Bmag0125-GBM1309 11.38 17.76 0.089
qTAC-4H 4H GBM1221-GBM1501 3.58 5.06 0.049
qTAC-6H 6H BMAG0807-GBM1212 4.08 6.49 0.054
qTAC-7H 7H EBmatc0016-Bmag0206 9.24 16.43 -0.085
千粒重
TKW		 qTKW-2Ha 2H Scssr03381-scssr07759 2.75 4.67 -1.434
qTKW-2Hb 2H Bmag0749-GBMS0128 4.59 7.70 -1.839
qTKW-7H 7H GMS056-GBM1303 18.73 34.51 -4.250
嵩明 Songming 2015 总花色苷
TAC		 qTAC-2H 2H GBM1121-Bmag0125 12.16 20.09 0.095
qTAC-6H 6H GBM1212-Bmag0173 3.77 6.24 0.053
qTAC-7H 7H GBM1516-EBmatc0016 12.01 23.86 -0.104
千粒重
TKW		 qTKW-2H 2H Scssr03381-scssr07759 2.50 5.39 -1.599
qTKW-7H 7H GMS056-GBM1303 17.37 35.37 -4.569

图2

大麦籽粒总花色苷含量和千粒重QTL 在染色体上的分布 三角形图案表示总花色苷含量QTL, 五角星图案表示千粒重QTL。白色、灰色和黑色图案分别表示在玉溪(2013年)、白邑(2014年)和嵩明(2015年)检测到的QTL。图中右侧标示的是2H和7H部分分子标记对应的物理位置。"

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