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作物学报 ›› 2020, Vol. 46 ›› Issue (11): 1810-1816.doi: 10.3724/SP.J.1006.2020.04080

• 研究简报 • 上一篇    

水杨酸提高甘草种子萌发和幼苗生长对盐胁迫耐性的效应

李润枝1(), 靳晴2, 李召虎2, 王晔1, 彭真1, 段留生1,2,*()   

  1. 1 北京农学院 / 农业应用新技术北京市重点实验室 / 植物生产国家级实验教学示范中心, 北京 102206
    2 中国农业大学农学院 / 植物生长调节剂教育部工程研究中心, 北京 100193
  • 收稿日期:2020-03-29 接受日期:2020-07-02 出版日期:2020-11-12 网络出版日期:2020-07-13
  • 通讯作者: 段留生
  • 作者简介:李润枝, E-mail:lirunzhi7639@163.com

Salicylic acid improved salinity tolerance of Glycyrrhiza uralensis Fisch during seed germination and seedling growth stages

LI Run-Zhi1(), JIN Qing2, LI Zhao-Hu2, WANG Ye1, PENG Zhen1, DUAN Liu-Sheng1,2,*()   

  1. 1 Beijing University of Agriculture / Beijing Key Laboratory of New Technology in Agricultural Application / National Demonstration Center for Experimental Plant Production Education, Beijing 102206, China
    2 College of Agronomy, China Agricultural University / Engineering Research Center of Plant Growth Regulator, Beijing 100193, China
  • Received:2020-03-29 Accepted:2020-07-02 Published:2020-11-12 Published online:2020-07-13
  • Contact: Liu-Sheng DUAN

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摘要：

本研究通过对甘草种子和幼苗进行水杨酸处理, 探讨在盐胁迫下水杨酸对其形态和生理生化指标的影响, 及其与耐盐性的关系。结果表明, 0.5 mmol L-1水杨酸处理甘草种子, 可以明显促进盐胁迫下(200 mmol L-1NaCl)甘草种子胚根伸长, 增加幼苗鲜重, 降低胚根中丙二醛(MDA)和脯氨酸含量, 提高过氧化物酶(POD)活性。在盐胁迫(100 mmol L-1和200 mmol L-1NaCl)条件下, 0.5 mmol L-1水杨酸喷施甘草幼苗, 可以降低幼苗中丙二醛(MDA)和脯氨酸含量, 不同程度的提高超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性。同时, 水杨酸处理甘草种子和幼苗, 可以显著增加盐胁迫下根中甘草酸含量。综上所述, 外施水杨酸可以缓解盐胁迫对甘草种子萌发的抑制作用, 提高抗氧化酶活性和降低膜脂过氧化程度, 提高了甘草种子和幼苗对盐胁迫的耐性。

关键词: 水杨酸, 甘草, 盐胁迫, 抗逆性

Abstract:

The present study investigated the effects of salicylic acid treatments on morphology, physiological and biochemical parameters and their relationship to salt stress of G. uralensis Fisch seeds and seedlings. Under the salt stress of 200 mmol L-1 NaCl, the application of 0.5 mmol L-1 salicylic acid on G. uralensis Fisch seeds could significantly promote the elongation of radicle, increase fresh weight of seedlings, reduce the content of malondialdehyde (MDA) and proline in the radicle, and improve the activity of peroxidase (POD). Under the salt stress (i.e. 100 mmol L -1 and 200 mmol L-1 NaCl), the application of 0.5 mmol L-1 salicylic acid on G. uralensis Fisch seedling could reduce MDA and proline content, and increase the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in different degrees. Under salt stress condition, the application of salicylic acid could increase the root glycyrrhizinic acid content. In summary, the application of salicylic acid could improve the tolerance to salt stress by alleviating the inhibition of salt stress on the germination of seeds, increasing the activity of antioxidant enzymes, and reducing the degree of membrane lipid peroxidation.

Key words: salicylic acid, Glycyrrhiza uralensis, salt stress, stress resistance

表1

水杨酸处理对盐胁迫下甘草苗鲜重的影响"

处理
Treatment		 处理时间 Treatment time
1 h 6 h 12 h 24 h 72 h
CK0 35.3±1.0 ab 43.6±2.3 a 44.5±2.4 a 48.5±2.6 a 62.4±3.6 a
SA0 36.4±1.5 a 44.7±1.7 a 45.2±2.5 a 49.1±1.9 a 64.1±2.1 a
CK200 32.3±1.6 c 33.2±0.9 c 34.8±2.1 c 37.4±1.0 c 46.8±2.0 c
SA200 34.0±1.4 bc 35.3±1.0 b 38.6±0.5 b 40.3±1.6 b 52.9±1.9 b

图 1

水杨酸处理对盐胁迫下甘草种子胚根长度的影响 处理同表1。柱上不同小写字母表示在0.05水平上有显著性差异。"

图 2

水杨酸处理对盐胁迫下甘草种子MDA(A)和脯氨酸(B)含量的影响 处理同表1。柱上不同小写字母表示在0.05水平上有显著性差异。MDA: 丙二醛。"

图 3

水杨酸处理对盐胁迫下甘草幼苗叶片中MDA含量的影响 CK0: 无SA处理+无盐胁迫; SA0: SA处理+无盐胁迫; CK100: 无SA处理+100 mmol L-1 NaCl溶液胁迫; SA100: SA处理+100 mmol L-1 NaCl溶液胁迫; CK200: 无SA处理+200 mmol L-1 NaCl溶液胁迫; SA200: SA处理+200 mmol L-1 NaCl溶液胁迫。MDA: 丙二醛。柱上不同小写字母表示在0.05水平上有显著性差异。"

图4

水杨酸处理对盐胁迫下甘草幼苗叶片中脯氨酸含量的影响 处理同图3。柱上不同小写字母表示在0.05水平上有显著性差异(P<0.05)。"

表2

水杨酸处理对盐胁迫下甘草种子氧化酶活性的影响"

处理
Treatment		 SOD活性
SOD activity (U g-1 FW)		 POD活性
POD activity (OD470 g-1 FW min-1)		 CAT活性
CAT activity (U g-1 FW min-1)
CK0 14±1.3 c 33±1.8 c 2.3±0.2 c
SA0 16±1.2 c 25±1.7 d 2.5±0.2 c
CK200 58±3.6 a 56±2.7 b 4.1±0.3 a
SA200 35±2.0 b 67±3.7 a 3.2±0.2 b

表3

水杨酸处理对盐胁迫下甘草幼苗叶片中氧化酶活性的影响"

处理
Treatment		 SOD活性
SOD activity (U g-1 FW)		 POD活性
POD activity (OD470 g-1 FW min-1)		 CAT活性
CAT activity (U g-1 FW min-1)
CK0 64.4±4.3 a 9.0±1.1 a 27.3±1.6 b
SA0 64.4±3.6 a 10.6±0.7 a 39.5±1.2 a
CK100 108.2±8.1 a 13.3±0.8 a 27.6±1.2 b
SA100 80.5±5.6 b 11.1±1.3 a 39.9±0.9 a
CK200 130.9±7.6 a 12.3±1.0 b 27.2±2.0 a
SA200 144.3±9.2 a 16.8±0.9 a 32.6±1.5 b

表4

水杨酸处理对盐胁迫下甘草种子甘草酸含量的影响"

处理
Treatment		 1 h 6 h 12 h 24 h 72 h
CK0 0.35±0.02 b 0.45±0.12 ab 0.73±0.09 ab 1.69±0.29 ab 4.62±0.52 b
SA0 0.45±0.03 a 0.65±0.12 a 0.95±0.10 a 1.89±0.36 a 5.81±0.32 a
CK200 0.19±0.01 c 0.25±0.07 b 0.38±0.15 b 0.79±0.04 c 2.64±0.21 c
SA200 0.33±0.03 b 0.43±0.11 ab 0.71±0.19 ab 1.18±0.09 b 3.86±0.45 b

图5

水杨酸处理对盐胁迫下甘草幼苗根中甘草酸含量的影响 处理同图3。柱上不同小写字母表示在0.05水平上有显著性差异(P < 0.05)。"

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