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作物学报 ›› 2021, Vol. 47 ›› Issue (2): 210-223.doi: 10.3724/SP.J.1006.2021.04086

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

抗根肿病甘蓝型油菜新品种华油杂62R的选育

李倩1(), Nadil Shah1, 周元委2, 侯照科1, 龚建芳1, 刘珏3, 尚政伟1, 张磊1, 战宗祥1, 常海滨4, 傅廷栋1, 朴钟云5,*(), 张椿雨1,*()   

  1. 1华中农业大学植物科学技术学院, 湖北武汉 430070
    2宜昌市农业科学研究院, 湖北宜昌 443000
    3德宏傣族景颇族自治州农业技术推广中心, 云南德宏 678400
    4黄冈市农业科学研究院, 湖北黄冈 438000
    5沈阳农业大学园艺学院, 辽宁沈阳 110000
  • 收稿日期:2020-04-02 接受日期:2020-07-02 出版日期:2021-02-12 网络出版日期:2020-07-11
  • 通讯作者: 朴钟云,张椿雨
  • 作者简介:E-mail: 2455302842@qq.com
  • 基金资助:
    国家重点研发计划项目(2016YFD0101300);国家现代农业产业技术体系建设专项(CARS-12)

Breeding of a novel clubroot disease-resistant Brassica napus variety Huayouza 62R

LI Qian1(), Nadil Shah1, ZHOU Yuan-Wei2, HOU Zhao-Ke1, GONG Jian-Fang1, LIU Jue3, SHANG Zheng-Wei1, ZHANG Lei1, ZHAN Zong-Xiang1, CHANG Hai-Bin4, FU Ting-Dong1, PIAO Zhong-Yun5,*(), ZHANG Chun-Yu1,*()   

  1. 1College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
    2Yichang Academy of Agricultural Sciences, Yichang 443000, Hubei, China
    3Dehong Dai Jingpo Autonomous Prefecture Agricultural Technology Extension Center, Dehong 678400, Yunnan, China
    4Huanggang Academy of Agricultural Sciences, Huanggang 438000, Hubei, China
    5College of Horticulture, Shenyang Agricultural University, Shenyang 110000, Liaoning, China
  • Received:2020-04-02 Accepted:2020-07-02 Published:2021-02-12 Published online:2020-07-11
  • Contact: PIAO Zhong-Yun,ZHANG Chun-Yu
  • Supported by:
    National Key Research and Development Program of China(2016YFD0101300);China Agriculture Research System(CARS-12)

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摘要：

我国油菜根肿病发病面积在66.7万公顷左右, 约占总生产面积的10%, 已严重威胁到油菜的安全生产。基于此, 本研究以含有CRb抗根肿病位点的大白菜材料CR Shinki为供体亲本, 以甘蓝型油菜国审杂交种华油杂62的父本Pol.CMS恢复系Bing409为受体亲本, 通过杂交、回交及自交等育种程序, 结合前景和遗传背景筛选, 将CRb抗病位点导入到Bing409中。在BC3F2代获得了遗传背景高度接近Bing409且含CRb抗病位点的抗根肿病新恢复系Bing409R, 进而成功选育了我国首个抗根肿病杂交油菜新品种华油杂62R。CRb抗病位点在甘蓝型油菜背景中表现为单基因显性遗传, 根肿病抗性遗传改良的同时并未对Bing409R及由其配制的杂交种华油杂62R的产量、品质造成不良影响, Bing409R及华油杂62R对我国四川、湖北、安徽等地区根肿菌生理小种具有免疫抗性。本研究的开展为我国油菜抗根肿病育种提供了宝贵资源, 为我国抵抗油菜根肿病的威胁提供了重要保障。

关键词: 甘蓝型油菜, 根肿病, CRb抗病位点, 恢复系, 分子改良, 华油杂62R

Abstract:

The rapeseed clubroot disease incidence in China is about 0.67 million hectare, accounting for 10% of the canola production area, which become a serious threat for the safety of Brassica napus industry. Based on this, we used CR Shinki, a Chinese cabbage material containing CRb clubroot disease resistance locus, as the donor parent, and Pol.CMS restorer line Bing409, the parent of Brassica napus national approved varieties Huayouza 62, as the recipient parent, and the CRb resistance locus was introduced into Bing409 by breeding programs such as crossing, backcrossing, self-cross with the foreground and genetic background selection. In the BC3F2 generation, a new restorer line Bing409R with a genetic background close to Bing409 containing CRb resistance locus was obtained, and Huayouza 62R, the first rapeseed hybrid resistant to clubroot disease in China was successfully developed. The results were as follows: CRb disease resistance locus appeared as a dominant single-gene inheritance in B. napus background, and the genetic improvement of resistance to clubroot disease did not at the expense of yield and quality losses for new restorer line Bing409R and its hybrid Huayouza 62R. Bing409R and Huayouza 62R were showed immune-resistance to physiological races of Plasmodiophora brassicae in Sichuan, Hubei, and Anhui provinces in China. This study will provide valuable resources for the breeding of rapeseed in China, and supplemented important support to overcome the threat of rapeseed clubroot disease.

Key words: Brassica napus, clubroot disease, CRb resistance locus, restorer line, genetic improvement, Huayouza 62R

附表1

用于遗传背景筛选的多态性分子标记"

染色体
Chr.		 标记名称
Marker name		 物理位置
Physical
location (Mb)		 正向引物
Forward primer (5°-3°)		 反向引物
Reverse primer (5°-3°)
A01 niab_ssr113 366,648 CAAAAAGTTGCGGTCAATCT CCTCCAAAGCTCAATCACTG
sau_um356 2,137,110 CATCTTCGTCTCTCCATCACCT GTATGGTAGGAGGAGAGTTCGCT
cnu_ssr134 4,481,886 TCTCTTTGCCATCGTCGTTTC CCCCTCAAACTGAGCAGTCAA
niab_ssr032 7,684,077 TTCTCCCCATCCTCTCATCTTA ACCCACAACCAACAAAATCTTC
cnu_mBRPGM0190 9,861,961 GAGATCCAATAGCGAGCACA TGTGTTATCGGGTGAAGTGG
染色体
Chr.		 标记名称
Marker name		 物理位置
Physical
location (Mb)		 正向引物
Forward primer (5°-3°)		 反向引物
Reverse primer (5°-3°)
A01 ACMP00456 13,955,674 CTCTACAAGCCGCAGAGAGA CATCCACCACAGAGATTGCT
ACMP00617 15,044,996 AACTCAATGCTCTTCGCTCA CCTTCTGCTGCTTCCAAGAT
ACMP00297 16,999,515 CAGATATTGCCCAAACACCA CTTCGCTTCTGAGCTTTCCT
sau_um586 20,719,752 AGGGTGATGATGATGTACAGGAG CTTCGTTAATTCTTCCACCCAG
cnu_ssr125 26,899,174 TCCAAGTGAAGGATAATGCTCGT TGTACAATGGGGATGTTGTGG
A02 sau-um619 1,404,160 GAGAGCTCACTTGTGCTTCTTGT CCGATCAAGTTATTCGCTCTCT
cnu_ssr116 3,025,687 GCCATGGTGGTGGGATTAGA TGGTCGAACGTGTGTCGATAAT
niab_ssr143 5,142,784 GATATGTTTCCAAACAAGTCAA AAATTCGACCCCTTTTCG
sau_um434 7,390,390 AGCTAGGGATGAGAAGAAGACCG TCTTTGTGTCCGTCAAGAGTCC
cnu_ssr036 10,214,155 AGCACAGTCAAAACTCAGAATGA AGGCATGCGCTCTCTATCTC
cnu_mBRPGM1391 11,315,073 GGTTTAGAGTGGTGCGGAAT CCGGGCCTAAAGTAATCAAA
cnu_mBRPGM0182a 14,588,355 TGGAGTAGAAGCGTCGTCTG CCTTCCTCCTCTCAGTTCCA
cnu_mBRPGM1527a 16,911,280 GCAAGCACGGAGACTAAACA GACGGTGAAGCTGTACGAAG
cnu_mBRPGM0813 18,824,804 GTTCCATAGGGCGTTCACAT CCGATGATTCTCTCAATCAGG
cnu_ssr447 21,870,396 TGGTGTCAAACGGACAGAAA ATACTCGGCTCAAACCGTCA
CB10416 23,408,087 GCTGTTGCTGTAGGTTTGA GAGCCAGCGTTGATAAGA
A03 niab_ssr115 1,049,474 CGGTGTATACCGAACGAGAA AAACCCAATCAACCCCTTTA
ACMP00292 2,920,902 GGGTTGCTGGTTTAGCTGTT TGAATCCGCTGAACTCTCTG
cnu_mBRPGM0240 5,317,208 GAGGGAAAGAGGACAATACGA TCATCGAGAAACGAAGGGTA
cnu_ssr173 8,444,756 TGTATTCCATTATTTCCGACTAACCT CCGCATTTTAAAAACGTGAGAAA
cnu_ssr290 9,860,984 CGATTTTGCCATTGTCTAAGC TGAAGACACGTTGGTTGAACA
ACMP00755 11,006,524 GTCATCGCAAGAGGACAAGA AAAGCTCCATCAAAGCACCT
cnu_ssr098 14,357,780 TGCGACCCAAGTAGGTGAAAC TGTCTCTCGCTCATTCATCCAA
cnu_ssr327 17,867,509 TTCTTGACCAAAAGAATCATGG CTAACACGGGGAAAAGCAGA
ACMP00186 19,379,932 GCCTCCCTGACTTGTACCTC TTCAATGCGCCAGTTAAGAC
ACMP00410 20,585,363 CTCAAAGCATGAACGTGGAC CCTCCCTTGATTTGTGGAGT
sau_um146 22,875,333 CTCGCAAAATCCCTTCTTCG CATATCGCTCGAGTTGCAGA
cnu_ssr526 23,351,679 TCCGAGAAGCACACAAAGAA TGACCATTTTCTGCCATTCA
TCR079 23,692,426 TGACGTTCAATCAAAGCCTGA TTTAGCAATCAAATGCAAATTCAA
cnu_ssr492 23,747,774 TCGAGGTGGTTACAATCCAA CAATGCGGATCTACCTCTCA
cra_id011 24,345,511 GTCGGATTTCTTTCTACACG TGAACCTATCTTCCTCAACG
cra_ssr015 24,345,899 CGGCCTCCGGAAATTTATTA TGGGAGGACCTCTCTCTTCTT
Cra_RT1 24,351,487 ACGTAAAGAAGCTGACCGGAGAC AGGCTTAACAACAGTTCCAGATT
cra_ssr017 24,355,996 GTGTGACCGCACTGTTGTTT AGTTTGACCCAAACGCATAA
sau_um026 25,648,956 AGTGGCTCCCAGGAGGATAATA CTTGGAGAAGAGAAACTTGGGC
cnu_ssr241 27,414,883 AATGCTGTGTCCATGACCAA CGGGCATCCACCTAATTTGT
At2g35530 29,980,641 CAGAGTAACTGGTTATGCCCGTC CAATAGGGATAAACCTGGAGACAG
ACMP00563 31,183,993 CGGAGATGGGATTAAGGAGA AAAGATCGTGTGGGTGGAAT
A04 BrID10929 251,988 CAATTTGGGAAGACAGTTCT GATTCGTTGATATAAGGCCA
BrID10321 2,417,875 TGTGTTTCCTAGTGTGTTGG ATCAGTCTGAGGGTTCATCA
BrID101249 3,990,390 TTGCATAGCACATGTAGGAG GAACGTCTACTTATGGAGAACA
BrID10645 7,441,204 GCAGAGGAAAACAATCAGAC AAGCGTCGACTTGAAATCT
ACMP00073 8,674,696 TTCAACCACACCGACAAACT CTGACGGAGTCCCTGTACCT
ACMP00356 11,811,660 TGAGGTCTACAGCCCAAGTG AATGGAGATCGTGTGCAAAG
染色体
Chr.		 标记名称
Marker name		 物理位置
Physical
location (Mb)		 正向引物
Forward primer (5°-3°)		 反向引物
Reverse primer (5°-3°)
A04 ACMP00744 12,860,981 CCTGTTCCAATCCCATTCTT AGATCCCTGACGGGTTTATG
ACMP00281 15,028,874 TCGACTTTGACGAAGACTGG AGTCCTCATGGCATCAAACC
hri_Mbrms195 17,105,559 AATACTTTCTGAAGTTGTCCGCTAA AACCTACGCAAGATGCTTCTACTT
cnu_ssr005 18,776,905 AGGAGTCTCGTTCCGTGAGA TTGAAATTAAGTCGAGCAAACAA
A05 cnu_ssr387 236,456 ACTCGAACCATTCTGGCAAA GGAACGACTTCCTCCCGTAT
niab_ssr017 2,501,334 GGTTAAGCAGACGATGGAAGTAA TATAGGGGAAATCATCTCAAGCA
cnu_ssr381 4,003,395 TGCTTTTAACCAAACTCAAACG TGCAGAGAGGCAAGTTTCAA
sau_um392 5,882,422 GCCAGTTTCGTCTTCTTCTCTG GAAGTCACACCCCCATCTCTATC
BrID90500 6,762,400 ATGGGCTTTGTTTGGGTAAA TGAAAGAGACAAGCTGGCAA
BrID10825 9,949,084 CAATGTGTTCGATGGAATG TAGTGTGCGAACAGATTCAG
ACMP00841 13,132,010 CAGTGGCAACAACATCAACA CTTCACATCGTCTCCACCAC
BrID101239 13,226,921 TCCACACAGAAGATAACTGGT GAGATGACATTTTCGCTGAC
cnu_ssr458 16,415,511 GGGGTGAATCTTGGATGAGG CTGACGGATTCCCAACGAAT
sau_um366 18,549,687 TTCTCCTCGTATCACCACTCCT GCCTACGTCTTCTACAGCGAGAT
niab_ssr082 20,079,421 CATTTCCCCGTGACTATCTG CGTCTTCATCTCAATCTCGC
sau_um551 21,619,807 GTCCATCTCCTACCTGCTCCA GTTTTGAGCCGAATAATGGTTG
hri_mBRMS007 23,268,922 AAATTGTTTCTCTTCCCCAT GTGTTAGGGAGCTGGAGAAT
A06 BrID10395 1,513,120 GCTGACATGTACCTTTTGAA CATCTAAGACCGAGTCAAGC
sau_um278 3,092,702 GAGAACAAGAGGAGGACGCTT CCGGAGGCAAGTATCCATAAG
niab_ssr134 5,621,445 CGCAGCCTTTTGCTTCT TTGCTCTCCTGCAGCTTG
cnu_mBRPGM1016a 6,689,917 TGGAGATGGCTGTTGTTGAT AGCAGATGTCGGGAATAACC
niab_ssr049 7,659,576 GAGGAATTAACGGCGTCTTG CAGTCGCCACTACCTGGTTT
ACMP00739 10,965,852 GGTGACTGTTCCTCATCAGC ATCCCTATCCAAACCTCTCG
BrID10847 14,198,854 TCATTGCCTTACTTTGTGAC CTGACACAGGTGAATCAACA
BrID10849 14,265,494 AAAGATCTGTGGAATCATGG GGCAAACATGGGTTGATA
BrID10627 15,630,795 AACACAGATCCAATCTAGCG GTCCTTAGGCCAAGCATT
ACMP00692 17,268,545 CGAGTTGCAGAGCCAAGTAG AACGTAACGCTTCCTCTTCC
sau-um121 18,555,402 GAACCTAACGAAAGGCACCAC AGTGAGGGTAGACAGGGAGAGAG
cnu_ssr220 21,896,695 ATCAGAACCGAATCCGACCA CAATGGTTGCAATGTTATTTGGA
sau-um415 22,971,940 ACGCAAAGAGAGCGAAAGAGTC GGTCTTAATCGCATGGAATCCG
sau-um616 23,447,329 ATTACCTATTGACCCCACACCAC GACGTAGAACAAGTGAGAAGGGA
A07 ACMP00261 1,018,274 AAGCCTCGACTTTGGAAGAT ATCTCCGTCTGGTCTCGTCT
At2g06510 2,516,307 GATCGGGTTAAGTCAGGACA ATGGTCTCCATGTTCAGCAC
ACMP00785 4,675,451 TGGCTCTGTTTCCTTCTCCT AAGGGATTGATCGGAACTTG
BrID10283 8,743,728 CGGTTAGGTCGTAACTCGTA CTCTTCATACGCAAGTCTTT
BrID10487 11,667,757 TCCCTTACAAGTTCATGGAT ATGGTAGCAACAAAACCAGT
nia_m063 12,010,219 GAAGAAACTCGGTGGGGAGT AAAGAGTTCCGAAAATGGGC
cnu_ssr511 14,701,383 TGTGGACGAGAAACTGAGGA TGAGATACTGGTGCGTGTGG
cnu_ssr044 16,404,302 TGTTTTGATCTTTACTGTTTTTGGA AATGTTTTTATATCACTATTGCCAAAT
cnu_ssr048 18,226,238 TTCTCCATGCTGTTCAATTCAC CATGAAAAATCGACCTTATTCCA
cnu_ssr516 20,127,600 ACTTGCCTTAGCCAGCAGCG AAGATTTTGTGTTGTGGTCTGGTGA
cnu_ssr1566 22,103,544 GTCAGACTCGGATGGCTTG TAGGAGCAGTTGGTTCAGCA
A08 ACMP00659 1,364,408 CCGCCTCAATCTCTAGCTTT CTCGTTCACCACCTCTGCTA
ACMP00551 2,716,097 CAGCAATGGTGGACATCTCT AACAAGACCGGAACCATCTC
染色体
Chr.		 标记名称
Marker name		 物理位置
Physical
location (Mb)		 正向引物
Forward primer (5°-3°)		 反向引物
Reverse primer (5°-3°)
A08 BrID90197 4,203,640 TGTTCAAACTTCCCACCCTC GTGAGCCCAAAAGTTCCTGA
BrID10839 6,657,012 AGGAACAATACCCATTTGTG GGTGTTTGTGTGTTCGGTA
BrID101199 7,356,083 ATGATGGAGATGGACATTTG TGTATCGGCAGAAGAATCTC
sau_um192 10,064,084 TCCCTCCTCTCTACGTCTTCTTC CTTCTCTGTAACGGGCTTTGAC
sau_um044 14,603,121 CGATTCATCCATCCATCACC CTATAGGACCGATCCCGTCTTT
cnu_ssr432 16,431,751 CAAACCTCGTCCTAAGCAGAA ACCTGAAGATGACCCAGACG
sau_um077 17,469,120 CTGATCCTCGAAGAAGACAGTGC CTTCATGCACTTGAGGAGTCG
cnu_ssr176 18,202,437 TGTAAGTCACGTTCGGTTTGCT AGGCATGTATGGAGATGTAGAGTGA
BRMS-198 21,521,373 CGAGAGCAGTTAGGAAGCTTATAGA AGAGATACTCTGTCCTCCACCTCTT
A09 nia_m010 1,815,031 GGTTGACGTCTCATTGTGTTCTT TAGCTTTGCTCACTTTTCACTCC
sau_um219 3,482,038 CGCAGCTTCCTCTGTATTGCTA GGCTCTCACCAGAGTCAAGTCTA
cnu_ssr157 5,671,382 CCGCAGTTGATCCATTAGCC ACGCTGCATCCACATGAAAC
sau_um368 6,944,590 AGCCCCGTCTCTTTCACTGTAT GATCTAGGGTCTCGTCGACTTTG
nia_m022 10,330,441 CTCTCGTCTCGGAGGATCTAAA GTGAGAGTGGTTGCTGAGTGAG
sau_um019 13,593,016 GGTCCTGCCATTCCTATTCTCT CATGCCACGTCAGCAATATG
sau_um138 16,538,853 CGCACACCATTTCCACAAAC GAGATGAATGTGCGTCTCCTG
ACMP00188 18,078,993 GATTCCTCTCCACGACCATT TCTCCCAAATCGGTTCTTTC
sau_um101 21,178,401 GATCTTATCGTGCCCATTGC CTCCTCATAGGGCTCCTTTTTC
cnu_ssr598 24,440,145 TTCACCGTCTGCTCTTATCG CTGCTCCCATACGATCCACT
cnu_ssr016 27,461,366 GGTGAATGGAATCTTGTCTTGA CCCAACAATCCCAGAAACAC
cnu_ssr119 30,057,081 ACACCTACTTGTTTCCATCCAAAT CGGGTATTTGCGTTGTTTCC
sau_um187 33,166,753 GTCCTCCTCAACCTCATCATCA AGTCGAGAGTAACGGGAAGAGAG
sau_um105 36,739,828 CCTTTCTAATGGGAAGCGGTAG CTCCCTCTTCGAATTGACTCAC
A10 sau_um126 2,528,134 AGAACACGCTCCTAACCATCAG TAGCTACGAGGCCTTAGAGGGTA
niab_ssr034 4,065,070 GTGCAAGTCAGTGCCAAAGA CTCGGTGGTTGAGTGAAGGT
sau_um433 7,037,138 AAGAGTCCACAGCAGGAGATTG GGGATGAGAAAAAGACAGGTGG
sau_um216 10,127,674 CTTTCTTCTCTCCGTCGTTCC AAGGTTAGGGTTAGAGAAGCCG
niab_ssr123 11,313,024 GGATCTAGAAACCCCTTCACA ATCTTGTGTCGGGCAGATAA
Nniab_ssr122 12,936,001 ACTTCTCCGGCTGGATACTC CCGTTTAAACTTGCGTTTGT
niab_ssr009 15,004,910 TTCCCAAGCTTGCTGGTACT GAGATTTCCCTCGCTTGATG
sau_um310 15,341,225 TCTTTTCCATCTCTCTACCATCATC CCTATGAGAGGAAGACCGAGACT

图1

华油杂62恢复系Bing409根肿病抗性遗传改良的技术路线"

表1

CRb抗病位点及Rfp恢复基因连锁标记"

标记名称
Marker name		 标记类型
Marker type		 正向引物
Forward primer (5°-3°)		 反向引物
Reverse primer (5°-3°)		 物理位置
Physical location (Mb)
CRb_ssr541 SSR TGCTTGAGCAGAAACAATATCAA TTGCGCATCTCTGTTTAGCTT A03: 23693689
CRb_ssr413 SSR ATTGTGCCGTCGGAATTAAA GATGATTAGAAAAGGTGTCTATTGC A03: 23762587
CRb_ssr01 SSR TCGAGGTGGTTACAATCCAA CAATGCGGATCTACCTCTCA A03: 24031219
Rfp_ssr52 SSR TCAACAACAACAGCCTTTCG GGAAGAAGTCGCTTCCTGTG A09: 34418209
Rfp_rt5 功能标记FM GGGATGCGATCCTGATATTTG GAGAGAGGCTACAGAACAAACT A09: 34485601

表2

均匀覆盖甘蓝型油菜A基因组的标记统计"

染色体
Chr.		 标记数目Marker
numbers		 标记间平均间距
Average distance of adjacent markers (Mb)
A01 10 2.95
A02 11 2.30
A03 22 2.01
A04 10 2.07
A05 13 1.92
A06 14 1.83
A07 11 2.11
A08 11 2.02
A09 14 2.69
A10 8 1.83
总数Total 124 —

附表2

F1、BC1及两亲本的发病率统计"

发病等级
Disease level		 调查株数Number of plants investigated
F1 BC1 CR Shinki Bing409
4级 Level 4 0 19 0 35
3级 Level 3 0 0 0 12
2级 Level 2 0 0 0 0
1级 Level 1 0 0 0 0
0级 Level 0 31 24 30 0

图2

F1及BC1抗病性鉴定 a: F 1接菌表型鉴定; b: F 1病情指数; c: BC1接菌表型鉴定; d: BC1病情指数; e: BC1基因型鉴定。A表示Bing409基因型, B表示CR Shinki基因型, H表示杂合基因型; S表示感病表型, R表示抗病表型。"

图3

BC1F1~BC3F1前景入选单株遗传背景回复率统计 图中括号里面的数字表示当代经过前景选择入选的含有前景抗病位点的单株数。"

表3

BC1~BC3各世代前景及背景选择概况"

世代
Generation		 抗病位点前景选择Foreground selection 背景筛选标记数
No. of markers used to background selection		 最终保留株数
No. of
selected
plants		 背景回复率
Genetic
background
recovery rates (%)		 用途
Application
检测株数
No. of
plants tested		 筛选标记数
No. of markers		 入选株数
No. of
selected plants
BC1F1 492 5 256 118 5 64-71 回交Backcross
BC2F1 232 5 107 36 4 73-93 回交Backcross
BC3F1 267 5 139 5 6 87-97 回交、自交
Backcross, self-cross

图4

BC3抗病性鉴定及恢复基因Rfp位点检测 a: BC3接菌表型鉴定; b: BC3病情指数; c: BC3基因型鉴定; d: BC3抗病植株恢复基因Rfp位点检测。A表示Bing409基因型, B表示CR Shinki基因型, H表示杂合基因型; S表示感病表型, R表示抗病表型。"

附表3

Bing409R抗病材料接种我国不同地区根肿菌株数统计"

根肿菌
P. brassica		 收集地点
Collection place		 Bing409R接菌株数Number of inoculated Bing409R plants (R/S) 对照
Control (R/S)
重复1 Repeat 1 重复2 Repeat 2 重复3 Repeat 3
Y-TC 云南腾冲Tengchong, Yunnan 15 (3/12) 24 (5/19) 26 (5/21) 19 (0/19)
Y-XC 云南楚雄Xiongchu, Yunnan 19 (16/3) 25 (19/6) 25 (21/4) 25 (0/25)
Y-BS 云南保山Baoshan, Yunnan 24 (5/19) 26 (6/20) 23 (4/19) 20 (0/20)
Y-LC 云南临沧Lincang, Yunnan 20 (11/9) 25 (15/10) 26 (16/10) 21 (0/21)
Y-DH 云南德宏Dehong, Yunnan 20 (4/16) 26 (6/20) 24 (5/19) 25 (0/25)
H-ES 湖北恩施Enshi, Hubei 17 (4/13) 26 (6/20) 20 (3/17) 22 (0/22)
H-YC 湖北宜昌Yichang, Hubei 23 (23/0) 22 (22/0) 26 (26/0) 22 (0/22)
H-ZJ 湖北枝江Zhijiang, Hubei 22 (22/0) 21 (21/0) 19 (19/0) 20 (0/22)
S-DY 四川德阳Deyang, Sichuan 26 (26/0) 22 (22/0) 24 (24/0) 22 (0/22)
S-PZ 四川彭州Pengzhou, Sichuan 27 (27/0) 22 (22/0) 23 (23/0) 20 (0/20)
S-GH 四川广汉Guanghan, Sichuan 25 (25/0) 23 (23/0) 23 (23/0) 25 (0/22)
S-CD 四川成都Chengdu, Sichuan 24 (24/0) 20 (20/0) 19 (19/0) 25 (0/22)
A-HS 安徽黄山Huangshan, Anhui 17 (17/0) 19 (19/0) 23 (23/0) 21 (0/22)

表4

Bing409R抗病材料对我国不同地区根肿菌的抗性评价"

根肿菌编号
No. of P. brassica		 收集地点
Collection locations		 409R相对病情指数Relative disease index of 409R 抗性评价
Resistance evaluation
重复1 Repeat 1 重复2 Repeat 2 重复3 Repeat 3
Y-TC 云南腾冲Tengchong, Yunnan 40 40 40 S (40±0)
Y-XC 云南楚雄Xiongchu, Yunnan 8 12 8 R (9±1)
Y-BS 云南保山Baoshan, Yunnan 40 38 41 S (40±1)
Y-LC 云南临沧Lincang, Yunnan 23 20 19 MS (23±1)
Y-DH 云南德宏Dehong, Yunnan 40 38 40 S (39±1)
H-ES 湖北恩施Enshi, Hubei 38 38 43 S (40±1)
H-YC 湖北宜昌Yichang, Hubei 0 0 0 I (0±0)
H-ZJ 湖北枝江Zhijiang, Hubei 0 0 0 I (0±0)
S-DY 四川德阳Deyang, Sichuan 0 0 0 I (0±0)
S-PZ 四川彭州Pengzhou, Sichuan 0 0 0 I (0±0)
S-GH 四川广汉Guanghan, Sichuan 0 0 0 I (0±0)
S-CD 四川成都Chengdu, Sichuan 0 0 0 I (0±0)
A-HS 安徽黄山Huangshan, Anhui 0 0 0 I (0±0)

表5

BC3F3不同抗病株系品质测定"

抗病株系
Resistant lines		 含油量
Oil content (%)		 芥酸
Erucic acid (%)		 硫甙
Glucosinolate (μmol g-1)
622-22 41.81 0.17 21.76
622-37 40.05 0 24.61
623-30 40.99 0 23.49
624-04 43.63 0.34 26.54
625-17 41.11 0 24.32
630-11 40.65 0 23.33
18ZP06 42.66 0 23.69
18ZP07 42.44 0 30.05
18ZP08 41.99 0.08 28.61
409S01 (CK) 41.06 0 22.76
409S02 (CK) 40.38 0 24.31
409S03 (CK) 43.78 0 23.78

图5

F1枝江病区表型鉴定 华油杂62R (F1)的抗病性明显优于其感病亲本Bing409S。"

图6

华杂62R新品种在枝江根肿病区的田间表现"

表6

抗根肿病杂交种华油杂62R主要产量性状考察及小区产量测定"

品种
Variety		 株高
Plant height (cm)		 有效分枝数
Effective branch number		 单株有效角果数
Silique number per plant		 每角粒数
Seeds per silique		 千粒重
Thousand-seed weight (g)		 单株产量
Yield per plant (g)		 小区产量
Yield per plot
(kg)
华油杂12
Huayouza 12		 170.8 5.6 162.2 20.9 3.41 11.6 6.367
华油杂62R
Huayouza 62R		 161.2 5.8 176.8 18.1 3.62 11.6 6.267
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