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作物学报 ›› 2021, Vol. 47 ›› Issue (4): 626-637.doi: 10.3724/SP.J.1006.2021.04145

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜早熟性状QTL定位及候选基因筛选

李书宇(), 黄杨, 熊洁, 丁戈, 陈伦林*(), 宋来强   

  1. 江西省农业科学院作物研究所, 江西南昌330200
  • 收稿日期:2020-07-02 接受日期:2020-10-14 出版日期:2021-04-12 网络出版日期:2020-11-11
  • 通讯作者: 陈伦林
  • 作者简介:E-mail: lishuyu0104@163.com
  • 基金资助:
    国家自然科学基金项目(31660403);国家重点研发计划项目(2017YFD0101703);国家现代农业产业技术体系建设专项(CARS-12)

QTL mapping and candidate genes screening of earliness traits in Brassica napus L.

LI Shu-Yu(), HUANG Yang, XIONG Jie, DING Ge, CHEN Lun-Lin*(), SONG Lai-Qiang   

  1. Institute of Crops, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, Jiangxi, China
  • Received:2020-07-02 Accepted:2020-10-14 Published:2021-04-12 Published online:2020-11-11
  • Contact: CHEN Lun-Lin
  • Supported by:
    National Natural Science Foundation of China(31660403);National Key Research and Development Program of China(2017YFD0101703);China Agriculture Research System(CARS-12)

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摘要：

目前对于油菜早熟的研究主要围绕开花期性状进行, 虽然开花期与生育期呈显著正相关, 但却并不完全一致。对于油菜开花后一系列生长发育进程相关性状的遗传研究和QTL定位鲜有报道。本研究以成熟期差异较大的2个油菜品种‘花前早’和‘Global’构建的DH群体为材料, 对影响油菜全生育期的各个发育阶段(开花期、花期持续时间、角果期持续时间和全生育期等)进行表型调查和QTL定位分析。共检测到30个早熟相关性状QTL位点, 其中开花期、花期持续时间、角果期持续时间和全生育期等分别检测到12、5、4和9个QTL位点, 解释了5.8%~22.4%的表型方差。发现4、2和1个全生育期QTL置信区间分别与开花期、花期持续时间、角果期持续时间位点置信区间完全或部分重叠。筛选到29个可能与油菜早熟性状相关的候选基因, 它们通过调控花期或籽粒发育等生长发育进程影响油菜早熟。因此, 在早熟性状的研究中, 可以同时从开花期和籽粒发育过程入手, 不但有利于使熟期进一步提前, 也可减缓早熟油菜品种过早开花导致的冬前低温寒潮天气的不利影响。

关键词: 甘蓝型油菜, 早熟, QTL作图, 候选基因

Abstract:

Currently, the research on earliness traits of rapeseed mainly focused on flowering time. Although there was a significant positive correlation between the flowering period and the growth period, it was not completely consistent. There are few reports on the genetic studies and QTL mapping of traits related to rapeseed growth and development after flowering. We carried out phenotypic survey (flowering time, flowering period duration, silique period duration etc.) and QTL mapping with the DH population constructed by ‘Huaqianzao’ and ‘Global’ as material. A total of 30 QTL loci for earliness traits were detected. Among them, 12, 5, 4 and 9 QTL loci were detected in the flowering time, flowering period duration, silique period duration and full growth period, respectively, explaining 5.8%-22.4% phenotypic variance. The 2, 4, and 1 QTL confidence intervals of full growth period were found overlap in part or in whole with flowering time, flowering period duration and silique period duration respectively. The 29 candidate genes were screened, which affected the earliness traits by regulating flowering or silique development in rapeseed growth and development processes. Therefore, in the study of earliness traits, we could consider flowering time and silique development process at the same time, which not only helps to advance the maturity period, but also reduces the adverse effects of early flowering.

Key words: Brassica napus L., earliness traits, QTL mapping, candidate genes

表1

亲本和DH群体在4个环境下的早熟相关性状表型"

环境
Environment		 研究材料
Material		 开花期
Flowering time		 花期持续时间
Flowering period duration		 角果期持续时间
Silique period
duration		 全生育期
Full growth period
2016南昌
2016 Nanchang		 亲本
Parents		 花前早 Huaqianzao 76.0 A 64 A 32.5 A 172.5 A
Global 138.5 B 36.5 B 30.0 A 205.0 B
DH群体
DH population		 最大值 Max. 141.0 77.0 36.0 205.0
最小值 Min. 76.0 26.0 22.0 172.5
均值 Mean 118.5 43.5 29.0 191.0
2016韶关
2016 Shaoguan		 亲本
Parents		 花前早 Huaqianzao 64.5 A 40.5 A 27.5 A 132.5 A
Global 123.5 B 22.5 B 37.5 B 183.5 B
DH群体
DH population		 最大值 Max. 131.0 58.5 44.5 185.0
最小值 Min. 58.0 17.0 11.0 124.0
均值 Mean 97.5 34.2 30.2 161.8
2018南昌
2018 Nanchang		 亲本
Parents		 花前早 Huaqianzao 108.0 A 46.0 A 29.0 A 183.0 A
Global 149.0 B 22 B 30.0 A 201.0 B
DH群体
DH population		 最大值 Max. 149.0 46.0 34.0 203.0
最小值 Min. 108.0 17.0 26.0 181.0
均值 Mean 138.1 26.3 30.1 194.5
2018西宁
2018 Xining		 亲本
Parents		 花前早 Huaqianzao 56.0 A 31.5 A 31.5 A 119.0 A
Global 72.5 B 41.0 B 16.0 B 129.5 B
DH群体
DH population		 最大值 Max. 98.0 42.0 31.5 135.5
最小值 Min. 56.0 18.0 9.5 116.5
均值 Mean 72.5 30.1 20.5 123.9

图1

DH群体早熟相关性状的频数分布图 2016 NC, 2016 SG, 2018 NC和2018 XN分别指2016年南昌, 2016年韶关, 2018年南昌和2018年西宁。"

表2

DH群体4个早熟性状的相关性分析"

开花期
Flowering time		 花期持续时间
Flowering period duration		 角果期持续时间
Silique period duration		 全生育期
Full growth period
开花期
Flowering time		 1 -0.880** 0.041 0.926**
花期持续时间
Flowering period duration		 1 -0.120 -0.701**
角果期持续时间
Silique period duration		 1 0.158*
全生育期
Full growth period		 1

表3

遗传连锁图谱信息统计表"

连锁群
Chr.		 连锁群总长度
Length (cM)		 标记数
No. of markers		 相邻标记间的最大间隔
Max. interval (cM)		 Bin数目
No. bins		 相邻bin间平均距离
Bin interval (cM)
A01 110.96 48,693 4.72 183 0.61
A02 117.38 44,545 5.95 201 0.59
A03 197.46 65,502 10.52 280 0.71
A04 119.03 43,644 6.56 190 0.63
A05 101.61 55,238 3.39 187 0.55
A06 107.28 43,735 4.37 197 0.55
A07 118.95 40,195 5.75 212 0.56
A08 62.56 38,113 1.50 151 0.42
A09 139.70 55,599 8.99 226 0.62
A10 95.92 44,019 3.39 185 0.52
C01 126.88 39,781 5.48 197 0.65
C02 148.14 83,307 8.73 201 0.74
C03 206.71 85,609 7.96 320 0.65
C04 195.49 37,202 9.81 248 0.79
C05 193.22 22,608 6.68 217 0.90
C06 161.24 27,401 17.33 165 0.98
C07 157.87 31,921 5.80 211 0.75
C08 133.07 36,408 5.50 194 0.69
C09 107.28 31,475 6.72 140 0.77
平均Average 136.88 46,052 6.80 206 0.67
合计Total 2600.73 874,995 — 3905 —

图2

遗传连锁图谱中的标记在各连锁群上的分布情况"

表4

不同环境下检测出的早熟相关性状QTL"

位点
Locus		 性状
Trait		 染色体
Chr.		 位置
Pos (cM)		 LOD值
LOD value		 贡献率
R2 (%)		 加性效应Additive effect 置信区间Confidence
interval		 环境
Environment
qFT.A02-1 FT A02 18.23 5.37 10.6 2.15 16.52-18.52 2018西宁 2018 Xining
qFT.A02-2 FT A02 60.99 3.99 9.1 -1.82 58.14-61.84 2018西宁 2018 Xining
qFT.A06-1 FT A06 95.90 6.73 10.9 2.39 95.61-96.75 2018南昌 2018 Nanchang
qFT.A09-1 FT A09 84.60 5.22 11.8 5.56 84.03-87.84 2016韶关 2016 Shaoguan
qFT.A09-2 FT A09 123.12 4.04 8.8 3.92 121.65-126.32 2016南昌 2016 Nanchang
qFT.C04-1 FT C04 99.26 4.32 9.5 5.04 96.73-99.26 2016韶关 2016 Shaoguan
qFT.C06-1 FT C06 145.13 5.90 9.6 2.29 144.13-149.13 2018南昌 2018 Nanchang
qFT.C07-1 FT C07 81.72 3.76 5.8 1.69 78.76-84.10 2018南昌 2018 Nanchang
qFT.C08-1 FT C08 15.37 7.47 12.4 2.05 10.88-15.37 2018西宁 2018 Xining
qFT.C08-2 FT C08 40.61 6.60 11.1 2.01 34.77-43.49 2018西宁 2018 Xining
FT C08 43.49 4.65 9.0 4.00 42.63-47.70 2016南昌 2016 Nanchang
FT C08 45.80 12.87 22.4 3.71 45.80-45.80 2018南昌 2018 Nanchang
qFT.C08-3 FT C08 52.89 6.54 12.5 6.50 52.04-53.75 2016韶关 2016 Shaoguan
qFT.C08-4 FT C08 104.06 4.57 7.2 2.08 103.27-104.94 2018南昌 2018 Nanchang
qFPD.A02-1 FPD A02 19.09 4.22 7.3 -1.40 19.09-21.67 2018南昌 2018 Nanchang
qFPD.A06-1 FPD A06 95.61 6.14 11.0 -1.54 95.33-97.32 2018南昌 2018 Nanchang
qFPD.A09-1 FPD A09 84.60 4.28 8.9 -2.21 84.03-87.25 2016韶关 2016 Shaoguan
qFPD.C04-1 FPD C04 123.90 3.79 8.4 -2.42 118.86-127.70 2016南昌 2016 Nanchang
qFPD.C08-1 FPD C08 45.80 8.24 15.2 -1.95 44.36-46.70 2018南昌 2018 Nanchang
qSPD.A09-1 SPD A09 91.99 4.59 9.1 -1.19 89.73-92.87 2018西宁 2018 Xining
qSPD.C04-1 SPD C04 64.02 5.07 10.3 -1.30 62.14-64.30 2018西宁 2018 Xining
qSPD.C08-1 SPD C08 17.20 4.77 10.2 1.71 13.88-21.20 2016韶关 2016 Shaoguan
qSPD.C08-2 SPD C08 32.35 5.86 10.1 -1.27 32.35-33.56 2018西宁 2018 Xining
qFGP.A02-1 FGP A02 50.67 5.50 10.7 -5.35 50.10-51.53 2016韶关 2016 Shaoguan
qFGP.A02-2 FGP A02 61.56 5.72 11.9 -1.53 58.71-62.13 2018西宁 2018 Xining
qFGP.A09-1 FGP A09 84.60 4.48 8.8 4.21 83.74-87.25 2016韶关 2016 Shaoguan
qFGP.C06-1 FGP C06 111.50 7.34 12.0 1.34 111.16-119.50 2018南昌 2018 Nanchang
qFGP.C07-1 FGP C07 79.33 6.53 10.0 1.16 78.14-80.33 2018南昌 2018 Nanchang
qFGP.C08-1 FGP C08 5.40 7.56 11.8 1.41 4.54-9.88 2018南昌 2018 Nanchang
qFGP.C08-2 FGP C08 23.27 6.16 12.5 2.15 21.20-27.89 2016南昌 2016 Nanchang
qFGP.C08-3 FGP C08 42.05 3.99 7.2 1.21 40.16-44.65 2018西宁 2018 Xining
FGP C08 44.07 5.42 10.7 4.84 43.49-44.65 2016韶关 2016 Shaoguan
qFGP.C08-4 FGP C08 86.77 5.97 9.2 1.21 86.77-87.36 2018南昌 2018 Nanchang

表5

早熟相关性状候选基因"

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