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作物学报 ›› 2022, Vol. 48 ›› Issue (5): 1222-1234.doi: 10.3724/SP.J.1006.2022.14060

• 耕作栽培·生理生化 • 上一篇    下一篇

不同施肥水平下甘蔗植株根系内生细菌群落结构特征

肖健1(), 陈思宇1, 孙妍1, 杨尚东1,*(), 谭宏伟2,*()   

  1. 1广西大学农学院, 广西南宁 530004
    2广西农业科学院 / 广西甘蔗遗传改良重点实验室, 广西南宁 530007
  • 收稿日期:2021-04-13 接受日期:2021-07-12 出版日期:2022-05-12 网络出版日期:2021-08-03
  • 通讯作者: 杨尚东,谭宏伟
  • 作者简介:E-mail: 1318513279@qq.com
  • 基金资助:
    国家重点研发计划项目(2020YFD1000505);国家自然科学基金项目(31760368);广西壮族自治区农业科学院广西甘蔗遗传改良重点实验室开放课题(16-K-04-01);国家现代农业产业技术体系建设专项(甘蔗宿根栽培, CARS170206);广西创新驱动发展专项(桂科AA17202042);广西甘蔗产业创新团队甘蔗栽培岗资助

Characteristics of endophytic bacterial community structure in roots of sugarcane under different fertilizer applications

XIAO Jian1(), CHEN Si-Yu1, SUN Yan1, YANG Shang-Dong1,*(), TAN Hong-Wei2,*()   

  1. 1Agricultural College, Guangxi University, Nanning 530004, Guangxi, China
    2Guangxi Key Laboratory of Sugarcane Genetic Improvement / Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
  • Received:2021-04-13 Accepted:2021-07-12 Published:2022-05-12 Published online:2021-08-03
  • Contact: YANG Shang-Dong,TAN Hong-Wei
  • Supported by:
    National Key Research and Development Program of China(2020YFD1000505);National Natural Science Foundation of China(31760368);Guangxi Zhuang Autonomous Region Academy of Agricultural Sciences Guangxi Sugarcane Genetic Improvement Key Laboratory Open Project(16-K-04-01);China Agricuture Research System (Sugarcane Host Cultivation, CARS170206);Guangxi Innovation-Driven Development Special Project (Guike AA17202042);Guangxi Sugarcane Industry Innovation Team Sugarcane Cultivation Post

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摘要：

分析不同施肥水平下甘蔗植株根系内生细菌群落结构特征, 旨在解析不同施肥水平对甘蔗植株内生细菌群落的影响, 为挖掘和利用有益内生细菌及其功能提供理论依据。结果发现, 门分类水平, 施肥处理条件下甘蔗植株根系中放线菌门(Actinobacteria)细菌丰度占比均低于相应的不施肥处理, 但绿弯菌门(Chloroflexi)细菌丰度占比均高于不施肥处理。属分类水平, Haliangium、Acidicapsa、unclassified_f__Ktedonobacteraceae和unclassified_f__ Acidobacteriaceae_Subgroup_1是常规施肥水平(3000 kg hm-2, H处理)条件下甘蔗植株根系特有的优势内生细菌属; 而鞘氨醇单胞菌属(Sphingomonas)是减量施肥水平(1500 kg hm-2, M处理)下特有的优势内生细菌属; 中慢生根瘤菌属(Mesorhizobium)、norank_f__BIrii41、糖霉菌属(Glycomyces)、norank_f__67-14、Reyranella、unclassified_f__ Steroidobacteraceae、unclassified_f__Xanthobacteraceae和norank_f__Roseiflexaceae、Dongia是低施肥水平处理(300 kg hm-2, L处理)甘蔗植株根系特有的内生细菌优势菌属; 柄杆菌属(Caulobacter)、假诺卡菌属(Pseudonocardia)、诺卡氏菌属(Nocardia)、Kitasatospora、norank_f__norank_o__norank_c__Actinobacteria、Oceanibaculum和拟无枝菌酸菌属(Amycolatopsis)是不施肥水平(CK)条件下甘蔗植株根系中特有的内生细菌优势属。基因功能预测结果显示, 与不施肥处理相比, 施肥不仅影响甘蔗植株根系内生细菌群落组成, 而且影响植株根系内生细菌的代谢功能; 但不同施肥水平处理之间, 依施肥量的多少对甘蔗植株根系内生细菌群落组成影响存在显著差异。与常规施肥相比, 减量施肥(1500 kg hm-2)处理不仅没有导致甘蔗产量显著降低, 而且没有显著改变常规施肥条件下甘蔗植株内生细菌的群落组成和代谢功能, 完全可替代常规施肥进行施肥管理。

关键词: 甘蔗, 减量施肥, 内生细菌, 高通量测序

Abstract:

To explore the beneficial endophytic bacteria in sugarcanes and their functions, we analyzed the characteristics of endophytic bacterial community structure in roots of sugarcane under different fertilizer applications. At the phylum level, the proportion of Actinobacteria in roots of sugarcanes under different fertilization levels were lower than that in the control. However, the proportion of Chloroflexi in roots of sugarcanes under different fertilization levels applications were higher than that in CK. At the genus level, Haliangium, Acidicapsa, unclassified_f__Ktedonobacteraceae, and unclassified_f__Acidobacteriaceae_ Subgroup_1 were the specific dominant endophytic bacterial genera in root of sugarcanes under conventional fertilization level (3000 kg hm-2, H treatment); while Sphingomonas was the specific dominant endophytic bacterial genus in roots of sugarcanes under fertilization level (1500 kg hm-2, M treatment); Mesorhizobium, norank_f__BIrii41, Glycomyces, norank_f__67-14, Reyranella, unclassified_f__Steroidobacteraceae, unclassified_f__Xanthobacteraceae, norank_f__Roseiflexaceae, and Dongia were the specific dominant endophytic bacterial genus in roots of sugarcanes under the fertilization level (300 kg hm-2, L treatment). On the contrary, Caulobacter, Pseudonocardia, Nocardia, Kitasatospora, norank_f__norank_o__norank_c__Actinobacteria, Oceanibaculum, and Amycolatopsis were the unique dominant endophytic bacterial genera in root of sugarcane without fertilization application (control). And Actinospica, Catenulispora, Acidothermus, Dyella, and norank_f__Xanthobacteraceae were lost in root of sugarcanes under low nitrogen applications. Gene function prediction also revealed that, compared to no-fertilization treatment, both the endophytic bacterial community structure and the metabolic functions of endophytic bacteria in roots of sugarcanes were affected by different fertilizer applications. However, the structures of endophytic bacterial community in roots of sugarcane were significant differences among different fertilization treatments depending on the amounts of fertilizer applications. Compared to conventional fertilization, the yields of sugarcanes, and the structure, metabolic function of endophytic bacteria in roots of sugarcanes were not significantly changed by fertilizer application under 1500 kg hm-2 treatment. In conclusion, the conventional fertilization at the present stage can be substituted by reducing fertilizer application from 3000 kg hm-2 to 1500 kg hm-2 level in sugarcane.

Key words: sugarcane (Saccharum L.), reduced fertilization, endophytic bacteria, high-throughput sequencing

表1

不同施肥水平下甘蔗产量"

样本
Sample name		 产量Yield
2018 2019 2020
CK 56.41±2.59 b 58.02±5.32 b 53.95±5.87 b
H 111.09±2.69 a 114.15±5.21 a 108.04±11.22 a
M 107.78±6.92 a 106.60±2.68 a 99.24±5.36 a
L 61.21±4.13 b 64.29±4.05 b 60.79±7.10 b

表2

不同施肥水平下甘蔗植株根系内生细菌不同分类水平数量"

样本
Sample		 分类操作单元数量
Number of operational
taxonomic units (OTU)		 不同分类阶元归类数量 Number of different taxonomic categories
门
Phylum		 纲
Class		 目
Order		 科
Family		 属
Genus		 种
Species
CK 406 14 26 73 110 185 276
H 569 18 34 84 138 231 372
M 570 19 31 84 136 234 362
L 641 20 35 93 152 266 419
总计 Total 963 20 40 105 184 338 565

表3

不同施肥水平下甘蔗植株根系内生细菌Alpha多样性"

样本
Sample name		 香农指数
Shannon index		 辛普森指数
Simpson index		 ACE指数
ACE index		 Chao1指数
Chao1 index
CK 3.41±0.02 c 0.082±0.012 a 414.08±29.20 b 309.04±19.74 b
H 4.05±0.06 ab 0.039±0.001 b 522.80±9.85 a 538.11±8.83 a
M 4.45±0.12 a 0.030±0.003 b 584.38±34.07 a 545.31±4.66 a
L 4.20±0.06 ab 0.046±0.017 ab 507.29±12.03 a 460.50±82.69 a

图1

不同施肥水平下甘蔗植株根系内生细菌门分类水平细菌组成 样本缩写同表1。"

图2

不同施肥水平下甘蔗植株根系内生细菌属分类水平细菌组成 样本缩写同表1。"

图3

不同施肥水平下甘蔗植株根系内生细菌属和种分类水平Venn图 样本缩写同表1。"

表4

预测功能基因在不同样品间的占比(一级功能层)"

一级功能层 Pathway level 1 H M L CK
代谢Metabolism 25.86 24.16 21.40 28.58
环境信息处理Environmental information processing 24.76 23.43 23.35 28.47
遗传信息处理Genetic information processing 27.02 24.70 21.22 27.05
细胞过程Cellular processes 24.65 24.85 22.42 28.09
人类疾病Human diseases 24.96 24.85 22.96 27.23
有机系统Organismal systems 26.23 23.71 21.30 28.76

图4

OTU水平不同施肥处理下甘蔗植株根系内生细菌COG功能分类 样本缩写同表1。"

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