作物学报 ›› 2023, Vol. 49 ›› Issue (1): 119-128.doi: 10.3724/SP.J.1006.2023.12089
赵凌(), 梁文化, 赵春芳, 魏晓东, 周丽慧, 姚姝, 王才林, 张亚东()
ZHAO Ling(), LIANG Wen-Hua, ZHAO Chun-Fang, WEI Xiao-Dong, ZHOU Li-Hui, YAO Shu, WANG Cai-Lin, ZHANG Ya-Dong()
摘要:
挖掘新的控制水稻抽穗期相关位点和候选基因, 对抽穗期的遗传机制研究和品种改良具有重要的意义。利用抽穗期存在明显差异的粳稻TD70和籼稻Kasalath杂交衍生的包含186个家系的重组自交系群体, 构建了基于深度重测序的高密度Bin遗传图谱, 图谱共包含12,328个Bin标记。RIL群体及亲本2018年和2021年正季种植于江苏省南京市江苏省农业科学院。以家系从播种到抽穗所经历的天数作为抽穗期表型值, 使用IciMapping软件3.4版的完备区间作图法, 对控制水稻抽穗期的QTL进行鉴定。2年共检测到15个抽穗期的QTL, 分布在3号、6号、7号、8号、10号和12号染色体上, LOD值为2.58~10.68, 其中7个QTL和已知抽穗期QTL的位置存在重叠或者部分重叠。共有4个QTL在2年均检测到, 表现出较强的稳定性。对2年重复鉴定到的4个QTL区间进行基因功能注释和亲本间序列分析, 共发现7个注释有功能, 且在2个亲本间编码区存在非同义突变的基因。根据候选基因SNP的类型对RIL群体家系进行基因等位型分类和效应分析, 发现4个基因其不同等位型的RIL家系在抽穗期上存在显著或者极显著差异, 推测可能为候选基因, 可用于后续水稻抽穗期的分子机制研究。
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