欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2023, Vol. 49 ›› Issue (4): 1132-1139.doi: 10.3724/SP.J.1006.2023.21021

• 研究简报 • 上一篇    下一篇

小麦种质资源苗期耐盐性鉴定评价

孙现军1(), 姜奇彦1, 胡正1, 李宏博2, 庞斌双2, 张风廷2, 张胜全2,*(), 张辉1,*()   

  1. 1中国农业科学院作物科学研究所作物种质资源中心, 北京 100081
    2北京市农林科学院杂交小麦研究所, 北京 100097
  • 收稿日期:2022-03-12 接受日期:2022-09-05 出版日期:2023-04-12 网络出版日期:2022-09-13
  • 通讯作者: *张胜全, E-mail: zsq8200@126.com;张辉, E-mail: zhanghui06@caas.cn
  • 作者简介:E-mail: sunxianjun@caas.cn
  • 基金资助:
    中国农业科学院科技创新工程(CAAS-ZDRW202201);北京市农林科学院科技创新能力建设专项(KJCX20210439);国家自然科学基金项目(31601329)

Identification and evaluation of wheat germplasm resources at seedling stage

SUN Xian-Jun1(), JIANG Qi-Yan1, HU Zheng1, LI Hong-Bo2, PANG Bin-Shuang2, ZHANG Feng-Ting2, ZHANG Sheng-Quan2,*(), ZHANG Hui1,*()   

  1. 1Center for Crop Germplasm Resources, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2Beijing Academy of Agriculture and Forestry Science Research Institute of Hybrid Wheat, Beijing 100097, China
  • Received:2022-03-12 Accepted:2022-09-05 Published:2023-04-12 Published online:2022-09-13
  • Contact: *E-mail: zsq8200@126.com;E-mail: zhanghui06@caas.cn
  • Supported by:
    Agricultural Science and Technology Innovation Program(CAAS-ZDRW202201);Science and Technology Innovation Project of BAAFS(KJCX20210439);National Natural Science Foundation of China(31601329)

RichHTML

24

PDF

636

摘要：

土壤盐渍化是影响小麦生长的重要非生物胁迫之一, 筛选培育耐盐小麦种质资源对开展盐碱地综合利用具有重要意义。本研究以19份杂交小麦和2份常规品种为试验材料, 以蛭石为培养基质, 设置NaCl含量分别为0、0.4%、0.8%、1.2%、1.6%、2.0%的6个处理, 从播种时开始盐胁迫处理, 分析测定生长相关的11项指标。采用多元统计分析方法对小麦种质资源进行苗期耐盐性评价, 结果表明, 在1.2%盐处理下, 小麦种质资源大多数指标的耐盐系数四分位差最大, 因此, 1.2%盐被认为是耐盐鉴定最适浓度; 利用主成分分析方法可将11项调查指标的耐盐系数简化为3个主成分; 通过主成分贡献率和隶属函数分析进一步将3个主成分值简化成综合评价指标D值; 根据D值利用聚类分析将21份小麦种质资源划分为5类, 对应高耐、耐盐、中耐、敏感和高敏5个耐盐级别, 苗期耐盐鉴定表明13份杂交小麦的综合评价D值高于捷麦19与济麦22; 结合逐步回归分析获得11个调查指标耐盐系数与D值的最优回归方程: D = -0.743+0.779×PLL+0.372×TNL+1.273×PH+0.336×PLC+0.279×RL+0.558×RDW, 由此回归方程可知倒二叶叶长(PLL)、总叶片数(TNL)、株高(PH)、倒二叶叶绿素(PLC)、根长(RL)和根干重(RDW)可作为1.2%持续盐胁迫下小麦种质资源苗期鉴定评价指标。

关键词: 小麦种质资源, 苗期耐盐, 综合评价

Abstract:

Soil salinization is one of the important abiotic stresses which affects wheat growth. The screening and breeding salt-tolerant wheat is of great significance to the utilization of salinized land. 19 hybrid wheat resources and 2 conventional varieties were used as the experimental materials, seeds were grown in vermiculite, 6 treatments of NaCl solutions with 0, 0.4%, 0.8%, 1.2%, 1.6%, and 2.0% from sowing were set, and 11 indicators related to the growth were analyzed and determined. The multivariate statistical analysis method was used to evaluate the salt-tolerant of wheat germplasm resources at seedling stage. The results showed that the saline tolerance coefficient of most indicators were the highest interquartile range under 1.2% salinity among germplasm resources, thus the 1.2% salinity was considered as the optimum concentration for identification saline tolerance. 11 indicators could be simplified into 3 principal components by using principal component analysis. Through the analysis of principal component contribution rate and membership function, the 3 principal component values were further reduced to D-value of comprehensive evaluation index. According to the D-value, 21 wheat germplasm resources were divided into 5 salt-tolerant levels (high tolerance, salt tolerance, medium tolerance, sensitive and high sensitivity) by cluster analysis. The comprehensive evaluation D-values of 13 hybrid wheat varieties were higher than that of Jiemai 19 and Jimai 22 at seedling stage. Combined with stepwise regression analysis, the optimal regression equation of salt-tolerant coefficient and D-value of 11 indicators was obtained: D= -0.743 + 0.779×PLL + 0.372×TNL + 1.273×PH + 0.336×PLC + 0.279×RL + 0.558×RDW. Based on the above regression equation, PLL (penultimate leaf length), PH (plant height), PLC (penultimate leaf chlorophyll), RL (root length), and RDW (root dry weight), could be used as evaluation indicators for wheat germplasm resources identification under 1.2% continuous salt stress at seedling stage.

Key words: wheat germplasm resources, salt-tolerant at seedling stage, comprehensive evaluation

附表1

小麦种质资源苗期耐盐鉴定评价体系要点汇总"

培养基质
Cultivation of
substrate		 初始处理期
Initial processing
period		 盐处理浓度
Salt treatment concentrations		 评价指标
Evaluation indicators		 持续时间
Time of duration (d)		 参考文献
Reference
萌发盘+水培
Germination box and water planting		 二叶期
Two leaves period		 1.2% 苗高 Plant height
主根长 Main root length
根数 Number of root
根鲜(干)重 Root fresh/dry weight
茎叶鲜(干)重 Stem fresh/dry weight
综合评价D值 Comprehensive evaluation D-value		 7 [2]
萌发盘+水培
Germination box and water planting		 发芽移栽后3 d
Three days after germination and transplanting		 1.0% 苗高 Plant height
根长 Root length
相对生长量 Relative growth rate		 7 [3]
萌发盘+水培
Germination box and water planting		 发芽移栽后3 d
Three days after germination and transplanting		 0.5%
1.0%
1.5%		 株高 Plant height
根长 Root length		 5 [4]
萌发盘+水培
Germination box and water planting		 三叶期
Three leaves period		 0.2 mol L-1
 生物量 Biomass
电导率 Conductivity
抗氧化酶类 Antioxidation enzymes		 7 [5]
萌发盘+水培
Germination box and water planting		 三叶期
Three leaves period		 0.3%
0.5%
1.0%		 叶绿素 Chlorophyll
抗氧化酶类 Antioxidation enzymes
 15 [6]
萌发盘+水培
Germination box and water planting		 一叶后6 d
Six days after one leaf period		 逐渐增加浓度
Concentration increased gradually
25 mmol L-1
75 mmol L-1
150 mmol L-1		 株高 Plant height
主根数 Number of main root
根长 Root length
根鲜(干)重 Root fresh/dry weight
茎叶鲜(干)重 Stem fresh/dry weight
耐盐指数 Index of salinity tolerance		 28 [7]
萌发盘+水培
Germination box and water planting		 二叶期
Two leaves period		 0.3 mol L-1
1.6 mol L-1		 生长指标 Growth index;
生理生化指标
Physiological and biochemical indexes
D值综合分析 Comprehensive evaluation D-value		 3 [8]
耕层土
Top soil		 三叶期
Three leaves period		 0.6%
0.8%
1.0%
1.2%		 株高 Plant height
茎叶鲜重 Fresh weight of stem and leaf
茎叶干重 Dry weight of stem and leaf
D值综合分析 Comprehensive evaluation D-value		 10 [9]
萌发盘+水培
Germination box and water planting		 二叶期
Two leaves period		 300 mmol L-1 单株调查耐盐表型
Salt tolerance phenotype of each plant		 20 [10]
萌发盘+水培
Germination box and water planting		 二叶期
Two leaves period		 处理液中含40%海水
Treatment solution contains 40% seawater		 株高 Plant height
根长 Root length
地上干重 Ground dry weight
地下干重 Root dry weight		 14 [11]
萌发盘+水培
Germination box and water planting		 二叶期
Two leaves period		 300 mmol L-1 单株调查耐盐表型
Salt tolerance phenotype of each plant		 20 [12]
萌发盘+水培
Germination box and water planting		 萌动发芽后
After germination
 逐渐提高盐浓度
Concentration increased gradually		 根长 Root length
株高 Plant height
根量 Number of root
分蘖数 Tiller number
叶片盐害症状 Symptom of leaf salt injury		 30 [13]

表1

不同盐胁迫下调查指标耐盐系数描述"

统计量
Statistics		 盐浓度Salinity (%) 总叶片数
TNL		 耐盐级别
STL		 株高
PH		 倒二叶
叶长
PLL		 倒二叶
叶宽
PLW		 倒二叶
叶绿素
PLC		 根长
RL		 地上鲜重
AFW		 地上干重
ADW		 根鲜重
RFW		 根干重
RDW
中位数
Median		 0.4 0.77 1.00 0.59 0.62 0.43 0.88 0.81 0.23 0.04 0.37 0.04
0.8 0.57 2.78 0.41 0.39 0.37 0.86 0.49 0.11 0.01 0.21 0.01
1.2 0.45 3.67 0.30 0.27 0.39 0.71 0.28 0.07 0.01 0.14 0.01
1.6 0.26 9.00 0.13 0.11 0.35 0.82 0.20 0.03 0 0.06 0.01
2.0 — — — — — — — — — — —
四分位差
Interquartile Range		 0.4 0.08 0.30 0.12 0.16 0.10 0.22 0.33 0.08 0.01 0.19 0.02
0.8 0.13 1.10 0.08 0.11 0.12 0.29 0.22 0.05 0.01 0.11 0.01
1.2 0.14 1.78 0.06 0.16 0.11 0.25 0.21 0.02 0.01 0.07 0
1.6 0.17 0 0.08 0.09 0.16 0.00 0.18 0.03 0.01 0.05 0.01
2.0 — — — — — — — — — — —

表2

前3个主成分特征值和特征向量描述"

性状
Trait		 主成分分析 Comprehensive indexes
F1 F2 F3
总叶片数 Total number of leaf 0.53 -0.11 0.57
耐盐级别 Salt tolerance level 0.44 0.66 -0.47
株高 Plant height 0.48 0.32 0.53
倒二叶长 Penultimate leaf length 0.59 0.70 0.15
倒二叶宽 Penultimate leaf width 0.60 -0.52 0.17
倒二叶叶绿素 Penultimate leaf chlprophyll 0.70 0.37 -0.45
根长 Root length 0.63 0.37 0.32
地上鲜重 Above-ground fresh weight 0.73 -0.27 -0.37
地上干重 Above-ground dry weight 0.54 -0.41 -0.30
根鲜重 Root fresh weight 0.78 -0.44 -0.12
根干重 Root dry weight 0.80 -0.27 0.13
特征值 Eigen values 4.36 2.07 1.44
贡献率 Contribution rate (%) 39.65 18.78 13.04
累计贡献率 Accumulative contribution rate (%) 39.65 58.43 71.48
权重 Weight (%) 55.47 26.28 18.25

表3

每份鉴定种质的主成分值、隶属函数值与综合评价D值"

种质
Germpalsm name		 主成分
Comprehensive index		 隶属函数
Membership function		 D值
D-value		 综合评价
Comprehensive
valuation
F1 F2 F3 R1 R2 R3
京麦13 Jingmai 13 1.48 1.06 2.55 0.76 0.77 1.00 0.81 高耐 High tolerance
BH1273 2.41 -0.42 -0.01 1.00 0.37 0.40 0.73 高耐 High tolerance
MS19-4 1.18 1.20 -0.34 0.69 0.80 0.33 0.65 耐盐 Salt tolerance
BH2528 1.03 0.45 -0.32 0.65 0.60 0.33 0.58 耐盐 Salt tolerance
BH6705 0.10 1.94 -1.43 0.41 1.00 0.07 0.51 耐盐 Salt tolerance
BH7805 0.55 -0.22 -0.59 0.53 0.42 0.27 0.45 中耐 Medium tolerance
BH1706 -0.15 -0.07 1.31 0.35 0.47 0.71 0.45 中耐 Medium tolerance
沧麦14 Cangmai 14 -0.44 0.62 0.62 0.28 0.65 0.55 0.42 中耐 Medium tolerance
CHA17-8 0.61 -0.13 -1.75 0.54 0.45 0.00 0.42 中耐 Medium tolerance
垦冬杂22号 Kendongza 22 0.51 -1.62 0.92 0.52 0.05 0.62 0.42 中耐 Medium tolerance
中种麦1731 Zhongzhongmai 1731 -0.77 0.58 1.05 0.19 0.64 0.65 0.39 中耐 Medium tolerance
BH5357 -0.62 -0.13 0.68 0.23 0.45 0.57 0.35 敏感 Sensitivity
CHA18-1 -1.25 1.66 -0.22 0.07 0.92 0.36 0.35 敏感 Sensitivity
捷麦19 Jiemai 19 0.16 -1.07 -0.62 0.43 0.20 0.26 0.34 敏感 Sensitivity
BH3757 -0.40 0.03 -0.80 0.29 0.49 0.22 0.33 敏感 Sensitivity
中种麦5363 Zhongzhongmai 5363 -0.93 0.60 -0.17 0.15 0.64 0.37 0.32 敏感 Sensitivity
西大7号Xida 7 -0.04 -1.07 -0.44 0.38 0.20 0.31 0.32 敏感 Sensitivity
沧麦6005 Cangmai 6005 -0.17 -0.73 -1.07 0.35 0.29 0.16 0.30 敏感 Sensitivity
济麦22 Jimai 22 -0.19 -1.82 -0.11 0.34 0.00 0.38 0.26 高敏 High sensitivity
BH5379 -1.53 -0.09 -0.16 0.00 0.46 0.37 0.19 高敏 High sensitivity
小偃60 Xiaoyan 60 -1.54 -0.77 0.89 0.00 0.28 0.61 0.19 高敏 High sensitivity

图1

基于D值的21份小麦种质资源聚类"

表4

回归方程的估计精度分析"

种质
Germplasm name		 D值
D-value		 预测D值
Predicted D-value		 预测差值
Difference		 预测精度
Evaluation accuracy (%)
BH1273 0.73 0.71 0.02 97.52
BH7805 0.45 0.45 0 99.68
MS19-4 0.65 0.65 0 99.63
BH6705 0.51 0.48 0.03 94.25
CHA18-1 0.35 0.39 -0.04 89.31
CHA17-8 0.42 0.41 0.01 97.98
BH5357 0.35 0.35 0 99.97
京麦13 Jingmai 13 0.81 0.82 -0.01 98.20
BH2528 0.58 0.59 -0.01 98.67
中种麦5363 Zhongzhongmai 5363 0.32 0.31 0.01 97.99
中种麦1731 Zhongzhongmai 1731 0.39 0.38 0.01 96.67
垦冬杂22号 Kendongza 22 0.42 0.42 0 99.64
西大7号 Xida 7 0.32 0.33 -0.01 96.74
济麦22 Jimai 22 0.26 0.27 -0.01 95.34
捷麦19 Jiemai 19 0.34 0.33 0.01 96.83
种质
Germplasm name		 D值
D-value		 预测D值
Predicted D-value		 预测差值
Difference		 预测精度
Evaluation accuracy (%)
沧麦14 Cangmai 14 0.42 0.43 -0.01 98.71
沧麦6005 Cangmai 6005 0.30 0.29 0.01 97.09
小偃60 Xiaoyan 60 0.19 0.18 0.01 95.40
BH5379 0.19 0.20 -0.01 94.33
BH3757 0.33 0.33 0 99.83
BH1706 0.45 0.45 0 99.99
[1] 王遵亲, 祝寿泉, 俞仁培. 中国盐渍土. 北京: 科学出版社, 1993.
Wang Z Q, Zhu S Q, Yu R P. Saline Soil in China. Beijing: Science Press, 1993. (in Chinese)
[2] 刘彤彤, 李宁, 魏良迪, 杨进文, 史雨刚, 王曙光, 孙黛珍. 山西省主推小麦品种芽期及苗期耐盐性的综合评价. 中国农业大学学报, 2022, 27(2): 22-33.
Liu T T, Li N, Wei L D, Yang J W, Shi Y G, Wang S G, Sun D Z. Comprehensive evaluation of salt tolerance of wheat varieties in Shanxi province during germination and seedling stage. J Chin Agric Univ, 2022, 27(2): 22-33. (in Chinese with English abstract).
[3] 韩冉, 解树斌, 李欣, 郭宪峰, 宫文萍, 汪晓璐, 刘爱峰, 李豪圣, 刘成, 刘建军. 耐盐小麦种质筛选、鉴定与评价. 山东农业科学, 2020, 52(9): 12-18.
Han R, Xie S B, Li X, Guo X F, Gong W P, Wang X L, Liu A F, Li H S, Liu C, Liu J J. Screening, identification and evaluation of salt-tolerant wheat germplasm. Shandong Agric Sci, 2020, 52(9): 12-18. (in Chinese with English abstract)
[4] 张巧凤, 陈宗金, 吴纪中, 蒋彦婕, 杨继书, 蔡士宾. 小麦种质芽期和苗期的耐盐性鉴定评价. 植物遗传资源学报, 2013, 14: 620-626.
Zhang Q F, Chen Z J, Wu J Y, Jiang Y J, Yang J S, Cai S B. Screening for salinity tolerance at germination and seedling stages in wheat germplasm. J Plant Genet Resour, 2013, 14: 620-626. (in Chinese with English abstract)
[5] 陈春舟, 马占军, 孟亚雄, 马小乐, 王化俊, 李葆春. 小麦种质资源抗旱耐盐性评价及种质筛选. 分子植物育种, 2021, 19: 4820-4835.
Chen C Z, Ma Z J, Meng Y X, Ma X L, Wang H J, Li B C. Evaluation and screening of wheat germplasm resources for drought and salt tolerance. Mol Plant Breed, 2021, 19: 4820-4835. (in Chinese with English abstract)
[6] 时丽冉, 白丽荣, 吕亚慈, 赵明辉, 赵凤梧, 李会敏. 小麦杂交品种衡9966苗期耐盐性分析. 作物杂志, 2018, (6): 149-153.
Shi L R, Bai L R, Lyu Y C, Zhao M H, Zhao F W, Li H M. Analysis of salt tolerance of wheat hybrid variety Heng 9966 at seedling stage. Crops, 2018, (6): 149-153. (in Chinese with English abstract)
[7] 马艳明, 刘敏, 冯魁, 颜国荣, 王莉, 肖菁, 刘宁. 新疆冬小麦选育品种苗期耐盐性鉴定. 新疆农业科学, 2017, 54: 1394-1401.
doi: 10.6048/j.issn.1001-4330.2017.08.003
Ma Y M, Liu M, Feng K, Yan G R, Wang L, Xiao Q, Liu N. Identification of salt tolerance of winter wheat breeding in Xinjiang in seedling stage. Xinjiang Agric Sci, 2017, 54: 1394-1401. (in Chinese with English abstract)
doi: 10.6048/j.issn.1001-4330.2017.08.003
[8] 张军, 刘英, 杨生鹏, 吴秀宁, 王新军, 陈新宏. 商洛主栽小麦品种苗期的耐盐性评价. 西北农业学报, 2017, 26: 1148-1155.
Zhang J, Liu Y, Yang S P, Wu X N, Wang X J, Chen X H. Evaluation of salt tolerance of main wheat varieties in Shangluo in seedling stage. Acta Agric Boreali-Occident Sin, 2017, 26: 1148-1155. (in Chinese with English abstract)
[9] 彭智, 李龙, 柳玉平, 刘惠民, 景蕊莲. 小麦芽期和苗期耐盐性综合评价. 植物遗传资源学报, 2017, 18: 638-645.
Peng Z, Li L, Liu Y P, Liu H M, Jing R L. Evaluation of salinity tolerance in wheat (Triticum aestium) gemotypes at germination and seedling stages. J Plant Genet Resour, 2017, 18: 638-645. (in Chinese with English abstract)
[10] 李剑峰, 樊哲儒, 张跃强, 王重, 张宏芝. 春小麦种质芽期和苗期耐盐性鉴定. 新疆农业科学, 2014, 51: 1583-1590.
Li J F, Fan Z R, Zhang Y Q, Wang Z, Zhang H Z. Screening for salt resistance at germination and seedling stages of spring wheat germplasm. Xinjiang Agric Sci, 2014, 51: 1583-1590. (in Chinese with English abstract)
[11] 吴纪中, 刘妍妍, 王冲, 沈振国, 蔡士宾, 张巧凤, 夏妍, 王桂萍, 陈亚华. 人工海水胁迫下小麦种质资源的耐盐性筛选与鉴定. 植物遗传资源学报, 2014, 15: 948-953.
Wu J Z, Liu Y Y, Wang C, Shen Z G, Cai S B, Zhang Q F, Xia Y, Wang G P, Chen Y H. Screening and identification of wheat germplasm for salt tolerance using artificial sea water. J Plant Genet Resour, 2014, 15: 948-953 (in Chinese with English abstract).
[12] 王萌萌, 姜奇彦, 胡正, 张辉, 樊守金, 冯沥, 张海玲. 小麦品种资源耐盐性鉴定. 植物遗传资源学报, 2012, 13: 189-194.
Wang M M, Jiang Q Y, Hu Z, Zhang H, Fan S J, Feng L, Zhang H L. Evaluation for salt tolerance of wheat cultivars. J Plant Genet Resour, 2012, 13: 189-194. (in Chinese with English abstract)
[13] 刘旭, 史娟, 张学勇, 马缘生, 贾继增. 小麦耐盐种质的筛选鉴定和耐盐基因的标记. 植物学报, 2001, 43: 948-954.
Liu X, Shi J, Zhang X Y, Ma Y S, Jia J Z. Screening salt tolerance germplasms and tagging the tolerance gene(s) using microsatellite (SSR) markers in wheat. Bull Bot, 2001, 43: 948-954. (in Chinese with English abstract)
[14] 马帅国, 田蓉蓉, 胡慧, 吕建东, 田蕾, 罗成科, 张银霞, 李培富. 粳稻种质资源苗期耐盐性综合评价与筛选. 植物遗传资源学报, 2020, 21: 1089-1101.
Ma S G, Tian R R, Hu H, Lyu J D, Tian L, Luo C K, Zhang Y X, Li P F. Comprehensive evaluation and selection of rice (Oryza sativa japonica) germplasm for saline tolerance at seedling stage. J Plant Genet Resour, 2020, 21: 1089-1101. (in Chinese with English abstract)
[15] 金杭霞, 郭丹丹, 杨清华, 郁晓敏, 傅旭军, 袁凤杰. 利用模糊隶属函数法综合评价大豆萌发期耐盐性. 分子植物育种, 2021, 19: 8265-8271.
Jin H X, Guo D D, Yang Q H, Yu X M, Fu X J, Yuan F J. Comprehensive evaluation of salt tolerance in soybean germination period by fuzzy subordinate function method. Mol PLant Breed, 2021, 19: 8265-8271. (in Chinese with English abstract)
[16] 刘丽丽, 乔梓启, 杜明, 王宝山. 一种基于综合指标的耐盐方程式准确预测玉米耐盐性. 植物生理学报, 2022, 58: 458-468.
Liu L L, Qiao Z Q, Du M, Wang B S. A salt tolerance equation based on comprehensive indexes accurately predicts salt tolerance of maize inbred lines. Plant Physiol Commun, 2022, 58: 458-468. (in Chinese with English abstract)
[17] 胡亮亮, 王素华, 王丽侠, 程须珍, 陈红霖. 绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选. 作物学报, 2022, 48: 367-379.
doi: 10.3724/SP.J.1006.2022.04283
Hu L L, Wang S H, Wang L X, Cheng X Z, Chen H L. Identification of salt tolerance and screening of salt tolerant germplasm of mungbean (Vigna radiate L.) at seedling stage. Acta Agron Sin, 2022, 48: 367-379. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2022.04283
[18] 宝力格, 陆平, 史梦莎, 许月, 刘敏轩. 中国高粱地方种质芽期苗期耐盐性筛选及鉴定. 作物学报, 2020, 46: 734-753.
doi: 10.3724/SP.J.1006.2020.94138
Bao L G, Lu P, Shi M S, Xu Y, Liu M X. Screening and identification of Chinese sorghum landraces for salt tolerance at germination and seedling stages. Acta Agron Sin, 2020, 46: 734-753. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2020.94138
[19] 巨伟, 田汝美, 吕娟, 赵凯, 李鑫, 栗红梅, 张友秋. 小麦耐盐性鉴定指标和方法的研究. 种子世界, 2014, (6): 20-21.
Ju W, Tian R M, Lyu J, Zhao K, Li X, Li H M, Zhang Y Q. Study on appraisal indicators and methods of wheat salt resistance. Seed World, 2014, (6): 20-21. (in Chinese with English abstract)
[20] 何晓群. 多元统计分析(第4版). 北京: 中国人民大学出版社, 2015. pp 152-174.
He X Q. Multivariate Statistical Analysis, 4th edn. Beijing: China Renmin University Press, 2015. pp 152-174. (in Chinese)
[1] 孟雨, 田文仲, 温鹏飞, 丁志强, 张学品, 贺利, 段剑钊, 刘万代, 郭天财, 冯伟. 基于不同发育阶段协同的小麦品种抗旱性综合评判[J]. 作物学报, 2023, 49(2): 570-582.
[2] 祝令晓, 宋世佳, 李浩然, 孙红春, 张永江, 白志英, 张科, 李安昌, 刘连涛, 李存东. 基于耐低氮综合指数的棉花苗期耐低氮品种筛选[J]. 作物学报, 2022, 48(7): 1800-1812.
[3] 胡亮亮, 王素华, 王丽侠, 程须珍, 陈红霖. 绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选[J]. 作物学报, 2022, 48(2): 367-379.
[4] 朱亚迪, 王慧琴, 王洪章, 任昊, 吕建华, 赵斌, 张吉旺, 任佰朝, 殷复伟, 刘鹏. 不同夏玉米品种大喇叭口期耐热性评价和鉴定指标筛选[J]. 作物学报, 2022, 48(12): 3130-3143.
[5] 宋丽君, 聂晓玉, 何磊磊, 蒯婕, 杨华, 郭安国, 黄俊生, 傅廷栋, 汪波, 周广生. 饲用大豆品种耐荫性鉴定指标筛选及综合评价[J]. 作物学报, 2021, 47(9): 1741-1752.
[6] 张鹤, 蒋春姬, 殷冬梅, 董佳乐, 任婧瑶, 赵新华, 钟超, 王晓光, 于海秋. 花生耐冷综合评价体系构建及耐冷种质筛选[J]. 作物学报, 2021, 47(9): 1753-1767.
[7] 郜欢欢,叶桑,王倩,王刘艳,王瑞莉,陈柳依,唐章林,李加纳,周清元,崔翠. 甘蓝型油菜种子萌发期耐铝毒特性综合评价及其种质筛选[J]. 作物学报, 2019, 45(9): 1416-1430.
[8] 纪龙,申红芳,徐春春,陈中督,方福平. 基于非线性主成分分析的绿色超级稻品种综合评价[J]. 作物学报, 2019, 45(7): 982-992.
[9] 崔翠,程闯,赵愉风,郜欢欢,王瑞莉,王刘艳,周清元. 52份豌豆种质萌发期耐铝毒性的综合评价与筛选[J]. 作物学报, 2019, 45(5): 798-805.
[10] 苑乂川, 陈小雨, 李明明, 李萍, 贾亚涛, 韩渊怀, 邢国芳. 谷子苗期耐低磷种质筛选及其根系保护酶系统对低磷胁迫的响应[J]. 作物学报, 2019, 45(4): 601-612.
[11] 张海燕,解备涛,汪宝卿,董顺旭,段文学,张立明. 不同甘薯品种抗旱性评价及耐旱指标筛选[J]. 作物学报, 2019, 45(3): 419-430.
[12] 李鹏程,毕真真,梁文君,孙超,张俊莲,白江平. DNA甲基化参与调控马铃薯干旱胁迫响应[J]. 作物学报, 2019, 45(10): 1595-1603.
[13] 王倩,崔翠,叶桑,崔明圣,赵愉风,林呐,唐章林,李加纳,周清元. 甘蓝型油菜种子萌发期耐苯磺隆种质筛选与综合评价[J]. 作物学报, 2018, 44(8): 1169-1184.
[14] 段文学,张海燕,解备涛,汪宝卿,张立明. 甘薯苗期耐盐性鉴定及其指标筛选[J]. 作物学报, 2018, 44(8): 1237-1247.
[15] 李龙,毛新国,王景一,昌小平,柳玉平,景蕊莲. 小麦种质资源抗旱性鉴定评价[J]. 作物学报, 2018, 44(7): 988-999.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2