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Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (04): 557-564.doi: 10.3724/SP.J.1006.2008.00557

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Expression of a Vacuolar Na+/H+ Antiporter Gene of Alfalfa Enhances Salinity Tolerance in Transgenic Arabidopsis

AN Bao-Yan12**,LUO Yan1**,LI Jia-Rui2,QIAO Wei-Hua3,ZHANG Xian-Sheng1*,GAO Xin-Qi1*   

  1. 1 State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an 271018, Shandong, China; 2 Department of Plant Pathology, Kansas State University, Manhattan 66506 KS, USA; 3 College of Life Sciences, Peking University, Beijing 100871, China
  • Received:2007-07-29 Revised:1900-01-01 Online:2008-04-12 Published:2008-04-12
  • Contact: ZHANG Xian-Sheng

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Abstract: Plant Na+/H+ antiporters play an important role in salt tolerance. An alfalfa (Medicago sativa) full length cDNA (named as MsNHX1) was isolated by RT-PCR according to the homologous region of plant Na+/H+ antiporter genes. Southern blot analysis revealed that there was a small antiporter gene family in alfalfa genome. Sequence analysis indicated that MsNHX1 shared high homology with other vacuolar Na+/H+ antiporters. Transient transfection of MsNHX1-GFP fusion gene revealed that MsNHX1 targeted to vacuolar membrane. The transcription of MsNHX1 was up-regulated after high salinity treatment. Furthermore, the expression of MsNHX1 in yeast partly suppressed the salt-sensitive phenotype of yeast Na+/H+ antiporter mutant, indicating that MsNHX1 was a functional Na+/H+ antiporter of alfalfa. The physiological analysis revealed that the expression of MsNHX1 in Arabidopsis enhanced the resistance of transgenic plants to salt stress. These results suggest that MsNHX1 is a vacuolar Na+/H+ antiporter and functions in salt tolerance of plants.

Key words: Alfalfa, Na+/H+ antiporter gene, Arabidopsis, Salt tolerance

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