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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 79-86.doi: 10.3724/SP.J.1006.2009.00079


Identification of 1BL/1RS Translocation Based on Mixograph Parameters in Common Wheat

LIU Jian-Jun1,XIAO Yong-Gui2,CHENG Dun-Gong1,LI Hao-Sheng1,LIU Li2,SONG Jian-Min1,LIU Ai-Feng1,ZHAO Zhen-Dong1,HE Zhong-Hu2,3*   

  1. 1 Crop Research Institute,Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2 Institute of Crop Sciences/National Wheat Improvement Centre/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences,Beijing 100081, China; 3 CIMMYT China Office,Beijing 100081,CHina
  • Received:2008-01-11 Revised:2008-05-29 Online:2009-01-12 Published:2008-11-17
  • Contact: HE Zhong-Hu
  • Supported by:



1BL/1RS translocation has been widely used for improving agronomic performance and disease resistance in wheat (Triticum aestivum L.), however, it has strong negative effect on processing quality. To develop a method for 1BL/1RS translocation identification with mixograph parameters, 404 advanced lines from 146 crosses in 2005–2006 (Experiment I) and 175 advanced lines and main cultivars of Shandong Province (Experiment II) in 2005–2006 and 2006–2007 cropping seasons were used in this study. All materials were sown under irrigation condition in a randomized complete design with 1 replication in Jinan. The genetic effect of 1BL/1RS translocation on mixograph parameters was investigated. The variations of mixograph parameters under different combinations of the high molecular weight glutenin subunits (HMW-GS) and low molecular weight glutenin subunits (LMW-GS) were also analyzed. 1BL/1RS translocation lines showed significantly shorter mixing time, less bandwidth of peak and bandwidth after 1 min peak, and higher angle of descent and the bandwidth ratio (the ratio of bandwidth of peak/bandwidth after 1 min peak) in comparison with non-1BL/1RS translocation lines. It indicated that the 1BL/1RS translocation has deleterious effects on mixograph parameters. Mixograph of the 1BL/1RS translocation was characterized with the bandwidth sharply declining and narrowing after 1 min peak, and increasing the bandwidth ratio, whereas the bandwidth of non-1BL/1RS translocations declined gently after 1 min peak or had a longer mixing tolerance, and had a little variation about the bandwidth ratio. Furthermore, 85.2% (Experiment I) and 96.8% (Experiment II) accuracies were achieved in grouping the 1BL/1RS translocation and non-1BL/1RS translocation on the basis of the band width ratio, i.e., 1BL/1RS translocation line had a value more than or equal to 1.6, and non-1BL/1RS translocation line had a value smaller than 1.6. Although the Glu-B3 alleles showed better quality parameters when HMW-GS 5+10 was presented, it was still the most unfavorable allele on mixograph parameters among all Glu-B3 alleles. Therefore, mixograph parameters could be used to determine the rheological properties and the presence of 1BL/1RS translocation.

Key words: Common wheat(Triticum aestivum L.), 1BL/1RS transloeation, Mixogram characteristics

[1]Ehdaie B, Whitkus R W, Waines J G. Root biomass, water-use efficiency, and performance of wheat-rye translocations of chromosomes 1 and 2 in spring bread wheat ‘Pavon’. Crop Sci, 2003, 43: 710–717
[2]Rabinovich S V. Importance of wheat-rye translocation for breeding modern cultivars of Triticum aestivum L. Euphytica, 1998, 100: 323–340
[3]Zhou Y(周阳), He Z-H(何中虎), Zhang G-S(张改生), Xia L-Q(夏兰琴), Chen X-M(陈新民), Gao Y-C(高永超), Jing Z-B(井赵斌), Yu G-J(于广军). Utilization of 1BL/1RS translocation in wheat breeding in China. Acta Agron Sin (作物学报), 2004, 30(6): 531–535(in Chinese with English abstract)
[4]Liu L(刘丽), Yan J(阎俊), Zhang Y(张艳), He Z-H(何中虎), Pe?a R J, Zhang L-P(张立平). Allelic variation at the Glu-1 and Glu-3 loci and presence of 1B/1R translocation, and their effects on processing quality in cultivars and advanced lines from autumn-sown wheat regions in China. Sci Agric Sin (中国农业科学), 2005, 38(10): 1944–1950(in Chinese with English abstract)
[5]Wieser H, Kieffer R, Lelley T. The influence of 1B/1R chromosome translocation on gluten protein composition and technological properties of bread wheat. J Sci Food Agric, 2000, 80: 1640–1647
[6]Liu J-J (刘建军), He Z-H(何中虎), Pe?a R J, Zhao Z-D(赵振东). The effects of 1B/1R translocation on grain quality and noodle quality of bread wheat. Acta Agron Sin (作物学报), 2004, 30(2): 149–153(in Chinese with English abstract)
[7]Burnett C J, Lorenz K J, Carver B F. Effects of the 1B/1R translocation in wheat on composition and properties of grain and flour. Euphytica, 1995, 86: 159–166
[8]Moreno-Sevilla B, Baenziger P S, Shelton D R, Graybosch R A, Peterson C J. Agronomic performance and end-use quality of 1B vs 1B/1R genotypes derived from the winter wheat “Rawhide”. Crop Sci, 1995, 35: 1607–1612
[9]Martin P, Carrillo J M. Cumulative and interaction effects of prolamin allelic variation and of 1BL/1RS translocation on flour quality in bread wheat. Euphytica, 1999, 108: 29–39
[10]Branlard G, Felix I. Part of the HMW glutenin subunits and omega gliadin allelic variants in the explanation of the quality parameters. In: Martino al Cimino S eds. Proceedings of International Meeting—Wheat Kernel Proteins: Molecular and Functional Aspects, Viterbo, Italy, 1994. pp 249–251
[11]Gianibelli M C, Larroque O R, MacRichie F, Wrigley C W. Biochemical, genetic, and molecular characterization of wheat glutenin and its component subunits. Cereal Chem, 2001, 78: 635–646
[12]Bietz J A, Wall J S. Isolation and characterization of gliadin-like subunits from glutenin. Cereal Chem, 1973, 50: 537–547
[13]Gupta R B, MacRitchie F. Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheats: II. Biochemical basis of the allelic effects on dough properties. J Cereal Sci, 1994, 19: 19–29
[14]Branlard G, Dardevet M, Saccomano R, Lagoutte F, Gourdon J. Genetic diversity of wheat storage proteins and bread wheat quality. Euphytica, 2001, 119: 59–67
[15]He Z H, Liu L, Xia X C, Liu J J, Pe?a R J. Composition of HMW and LMW glutenin subunits and their effects on dough properties, pan bread, and noodle quality of Chinese bread wheats. Cereal Chem, 2005, 82: 345–350
[16]Kolster P, Krechting C F, van Gelder W M J. Quantification of individual high molecular weight subunits of wheat glutenin SDS-PAGE and scanning densitometry. J Cereal Sci, 1992, 15: 49–61
[17]Wang F-C(王凤成), Zhang W(张玮), Chen W-Y(陈万义). Mixograph and its application in the determination of wheat flour quality. Cereal & Feed Ind, 2004, 12: 10–12
[18]Lagudah E S, Appels R, McNeil D. The Nor-D3 locus of Triticum tauschii: natural variation and genetic linkage to markers in chromosome 5. Genome, 1991, 34: 387–395
[19]Francis H A, Leitch A R, Koebner R M D. Conversion of a RAPD-generated PCR product, containing a novel dispersed repetitive element, into a fast and robust assay for the presence of rye chromatin in wheat. Theor Appl Genet, 1995, 90: 636–642
[20]Xiao Y-G(肖永贵), Yan J(阎俊), He Z-H(何中虎), Zhang Y(张勇), Zhang X-K(张晓科), Liu L(刘丽), Li T-F(李天富), Qü Y-Y(曲延英), Xia X-C(夏先春). Effect of 1BL/1RS translocation on yield traits and powdery mildew resistance in common wheat and QTL analysis. Acta Agron Sin (作物学报), 2006, 32(11): 1636–1641(in Chinese with English abstract)
[21]Liu L(刘丽), Zhou Y(周阳), He Z-H(何中虎), Yan J(阎俊), Zhang Y(张艳), Pe?a R J. Effect of allelic variation at Glu-1 and Glu-3 loci on processing quality in common wheat. Acta Agron Sin (作物学报), 2004, 30(11): 959–968(in Chinese with English abstract)
[22]Graybosch R A. Uneasy Unions: Quality effects of rye chromatin transfer to wheat. J Cereal Sci, 2001, 33: 3–16
[23]Fenn D, Lukow O M, Bushuk W, DePauw R M. Milling and baking quality of 1BL/1RS translocation wheat: I. Effects of genotype and environment. Cereal Chem, 1994, 71: 189–195
[24]Zarco-Hernandez J A, Santiveri F, Michelena A, Pe?a R J. Durum wheat (Triticum turgidum L.) carrying the 1BL/1RS chromosomal translocation: agronomic performance and quality characteristics under Mediterranean conditions. Eur J Agron, 2005, 22: 33–43
[25]Finney K F, Shogren M D. A ten-gram mixograph for determining and predicting functional properties of wheat flours. Baker’s Digest, 1972, 46: 32–77
[26]Randall P G, Manley M, McGill A E, Taylor J R N. Relationship between high Mr subunits of glutenin of South African wheats and end-use quality. J Cereal Sci, 1993, 18: 251–258
[27]Lee J H, Graybosch R A, Peterson C J. Quality and biochemical effects of a 1BL/1RS wheat-rye translocation in wheat. Theor Appl Genet, 1995, 90: 105–112
[28]Pe?a R J, Amaya A, Rajaram S, Mujeep-Kazi A. Variation in quality of characteristics associated with some spring 1B/1R translocation wheats. J Cereal Sci, 1990, 12: 105–112
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