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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 79-86.doi: 10.3724/SP.J.1006.2009.00079

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of 1BL/1RS Translocation Based on Mixograph Parameters in Common Wheat

LIU Jian-Jun1,XIAO Yong-Gui2,CHENG Dun-Gong1,LI Hao-Sheng1,LIU Li2,SONG Jian-Min1,LIU Ai-Feng1,ZHAO Zhen-Dong1,HE Zhong-Hu2,3*   

  1. 1 Crop Research Institute,Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2 Institute of Crop Sciences/National Wheat Improvement Centre/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences,Beijing 100081, China; 3 CIMMYT China Office,Beijing 100081,CHina
  • Received:2008-01-11 Revised:2008-05-29 Online:2009-01-12 Published:2008-11-17
  • Contact: HE Zhong-Hu
  • Supported by:

    本研究由国家科技支撑计划项目（2006BAD01A02-14），国家公益性行业（农业）科研专项经费项目（nyhyzx07-002)，农业部引进国际先进农业科学技术计划（948计划）项目（2006-G2），山东省三O项目，山东省泰山学者项目资助

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Abstract:

1BL/1RS translocation has been widely used for improving agronomic performance and disease resistance in wheat (Triticum aestivum L.), however, it has strong negative effect on processing quality. To develop a method for 1BL/1RS translocation identification with mixograph parameters, 404 advanced lines from 146 crosses in 2005–2006 (Experiment I) and 175 advanced lines and main cultivars of Shandong Province (Experiment II) in 2005–2006 and 2006–2007 cropping seasons were used in this study. All materials were sown under irrigation condition in a randomized complete design with 1 replication in Jinan. The genetic effect of 1BL/1RS translocation on mixograph parameters was investigated. The variations of mixograph parameters under different combinations of the high molecular weight glutenin subunits (HMW-GS) and low molecular weight glutenin subunits (LMW-GS) were also analyzed. 1BL/1RS translocation lines showed significantly shorter mixing time, less bandwidth of peak and bandwidth after 1 min peak, and higher angle of descent and the bandwidth ratio (the ratio of bandwidth of peak/bandwidth after 1 min peak) in comparison with non-1BL/1RS translocation lines. It indicated that the 1BL/1RS translocation has deleterious effects on mixograph parameters. Mixograph of the 1BL/1RS translocation was characterized with the bandwidth sharply declining and narrowing after 1 min peak, and increasing the bandwidth ratio, whereas the bandwidth of non-1BL/1RS translocations declined gently after 1 min peak or had a longer mixing tolerance, and had a little variation about the bandwidth ratio. Furthermore, 85.2% (Experiment I) and 96.8% (Experiment II) accuracies were achieved in grouping the 1BL/1RS translocation and non-1BL/1RS translocation on the basis of the band width ratio, i.e., 1BL/1RS translocation line had a value more than or equal to 1.6, and non-1BL/1RS translocation line had a value smaller than 1.6. Although the Glu-B3 alleles showed better quality parameters when HMW-GS 5+10 was presented, it was still the most unfavorable allele on mixograph parameters among all Glu-B3 alleles. Therefore, mixograph parameters could be used to determine the rheological properties and the presence of 1BL/1RS translocation.

Key words: Common wheat(Triticum aestivum L.), 1BL/1RS transloeation, Mixogram characteristics

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