Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (4): 615-619.doi: 10.3724/SP.J.1006.2009.00615

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

SSR Analysis of Funo Wheat and Its Derivatives

SI Qing-Lin12,LIU Xin-Lun1,LIU Zhi-Kui3,WANG Chang-You1,JI Wan-Quan1*   

  1. 1College of Agronomy,Northwest A&F University,Yangling 712100,China;2Institute of Jiyuan Agricultual Science, JiYuan 454652,China;3Seed Management Station of Xi'an City,Xi'an 710054,China
  • Received:2008-09-22 Revised:2008-10-15 Online:2009-04-12 Published:2009-02-13
  • Contact: JI Wan-Quan E-mail:jiwanquan2003@126.com

Abstract:

Funo is one of the most important backbone parents in China, and many wheat (Triticum aestivum L.) varieties are derived from it. Funo and 8 parents of the first generation of Funo were analyzed using 247 simple sequence repeat (SSR) markers that covered the entire wheat genome to disclose the inheritance of genes in Funo on genome level and provide theoretical basis on development of backbone parent in wheat. Specific DNA fragments were detected using three SSR markers, Xwmc398, Xgwm400, and Xgwm268. The specific fragments were 178 and 151 bp for Xwmc398, 180 and 149 bp for Xgwm400, and 191 bp for Xgwm268. A total of 255 derivatives of Funo including 54, 124, 36, 33, 5, and 3 varieties respectively from the first to the sixth generations and 5 selected strains of Funo were analyzed using the specific primers. The specific bands of Xwmc398 were observed in all the five selected strains of Funo, whereas, only in Anxuan 2 for Xgwm400 and in Yangmai 1 for Xgwm268,. In the 255 varieties derived from Funo, the inheritable rates of specific bands for Xwmc398 were 52.8%, 38.4%, 16.7%, 0.0%, 0.0%, and 0.0% from the first to the sixth generations, respectively, with an average rate of 32.2%. For Xgwm400, the rates were 32.1%, 19.2%, 41.7%, 33.3%, 20.0%, and 0.0%, with an average of 26.7%. For Xgwm268, the rates were 22.6, 34.4, 11.1, 12.1, 0.0, and 0.0%, respectively, with an average of 24.7%. Xwmc398 had the highest inheritable rate in the Funo-derivative varieties and the most stable inheritance in Funo-selective varieties.

Key words: Funo pedigree, Backbone parent, Specific band of SSR marker

[1] Wang S-S(王珊珊), Li X-Q(李秀全), Tian J-C(田纪春). Genetic diversity of main parent of wheat ‘Aimengniu’and its pedigree on SSR markers. Mol Plant Breed (分子植物育种), 2007, 5(4): 485–490 (in Chinese with English abstract)
[2] Qiu F-L(邱福林), Zhuang J-Y(庄杰云), Hua Z-T(华泽田), Wang Y-R(王彦荣), Cheng S-H(程式华). Inspect of genetic differentiation of main parents of japonica hybrid rice in the northern China by simple sequence repeats (SSR). Chin J Rice Sci (中国水稻科学), 2005, 19(2): 101–104 (in Chinese with English abstract)
[3] Zhang X-Y(张学勇), Tong Y-P(童依平), You G-X(游光霞), Hao C-Y(郝晨阳), Gai H-M(盖红梅), Wang L-F(王兰芬), Li B(李滨), Dong Y-C(董玉琛), Li Z-S(李振声). Hitchhiking effect mapping: a new approach for discovering agronomic important genes. Sci Agric Sin (中国农业科学), 2006, 39(8): 1526–1535 (in Chinese with English abstract)
[4] Duan S-H(段世华), Mao J-N(毛加宁), Zhu Y-G(朱英国). Genetics analysis and identification of hybrid rice HL-type (Honglian-2) and their backbone parental with RAPD markers. J Wuhan Bot Res (武汉植物学研究), 2002, 20(3): 171–176 (in Chinese with English abstract)
[5] Zhang K-Z(张楷正), Ming H-M(明红梅), Li P(李平). Identification and analysis of core rice parents’ resistance to rice sheath blight in south rice production regions of China. Plant Prot (植物保护), 2008, 34(1): 45–48 (in Chinese with English abstract)
[6] Zhao Y-Z(赵一洲), Wang S-L(王绍林), Zhang Z(张战). Analysis on breeding value primal parents in rice. Reclaiming & Rice Cult (垦殖与稻作), 2008, (4): 6–9 (in Chinese with English abstract)
[7] Zhan K-H(詹克慧), Gao X(高翔), Fan P(范平), Xu H(许海), Ren G-M(任国民), Wu X-T(吴秀婷). An analysis of the corner stone parents for registered wheat (Triticum aestivum L.) cultivars in Henan province. J Henan Agric Univ (河南农业大学学报), 2006, 40(1): 11–14 (in Chinese with English abstract)
[8] Zhuang Q-S(庄巧生). Chinese Wheat Improvement and Pedigree Analysis (中国小麦品种改良及系谱分析). Beijing: China Agriculture Press, 2003 (in Chinese)
[9] Jin S-B(金善宝). Chinese Wheat and Pedigree Analysis (中国小麦品种及其系谱). Beijing: Agriculture Press, 1983 (in Chinese)
[10] Yang H-A(杨华安), Stubbs R. W. Gene postulation for wheat stripe rust resistance on Chinese differential hosts. Acta Phytophylacica Sin (植物保护学报), 1990, 17(1): 67–72 (in Chinese with English abstract)
[11] McIntosh R A. Catalogue of gene symbols for wheat. In: Proceedings of the 9th International Wheat Genetics Symposium. 1998. pp 139–141
[12] Ma L-L(马蕾蕾), Wang R-Y(王瑞义), Wu Y-X(吴玉星), Lin R-M(蔺瑞明), Xu S-C(徐世昌). Monosomic analysis of the resistant genes of Chinese differential-Funo to Puccinia striiformis. Plant Prot (植物保护), 2006, 32(1): 27–29 (in Chinese with English abstract)
[13] Saghai-Maroof M A, Soliman K, Joregensen R A, Allard R W. Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA, 1984, 81: 8014–8018
[14] Roder M S, Korzum V, Wendehake K, Plaschke J, Tixier M H, Lnoy P, Ganal M W. A microsatellite map of wheat. Genetics, 1998, 149: 2007–2023
[15] Guyomarc’h H, Sourdille P, Charmet G, Edwards K J, Bernard M. Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor Appl Genet, 2002, 104: 1164–1172
[16] Somers D J, Isaac P, Edwards K. A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet, 2004, 109: 1105–1114
[17] Wang C Y, Ji W Q, Zhang G S, Wang Q Y, Cai D M, Xue X Z. SSR markers and preliminary chromosomal location of a powdery mildew resistance gene in common wheat germplasm N9134. Acta Agron Sin (作物学报), 2007, 33(1): 163–166
[18] Zhou Y(周阳), He Z-H(何中虎), Zhang G-S(张改生), Xia L-Q(夏兰琴), Chen X-M(陈新民), Zhang L-P(张立平), Chen F(陈锋). Rht8 dwarf gene distribution in Chinese wheats identified by microsatellite markers. Acta Agron Sin (作物学报), 2003, 29(6): 810–814 (in Chinese with English abstract)
[1] XIE Hui,DANG Xiao-Jing,LIU Er-Bao,ZENG Si-Yuan,HONG De-Lin. Identifying SSR Marker Locus Genotypes with Elite Combining Ability for Yield Traits in Backbone Parents of Japonica Hybrid Rice (Oryza sativa L.) in Jianghuai Area [J]. Acta Agron Sin, 2016, 42(03): 330-343.
[2] ZHAO Chun-Hua,FAN Xiao-Li,WANG Wei-Lian,ZHANG Wei,HAN Jie,CHEN Mei,JI Jun,CUI Fa, LI Jun-Ming. Genetic Composition and Its Transmissibility Analysis of Wheat Candidate Backbone Parent Kenong 9204 [J]. Acta Agron Sin, 2015, 41(04): 574-584.
[3] SUN Zong-Xiu**,*,E Zhi-Guo**,WANG Lei,ZHU De-Feng,ZHANG Yu-Ping,HU Guo-Cheng,LIU Wen-Zhen,FU Ya-Ping*. Exploring Assessment Method of Chinese Rice Backbone Parents [J]. Acta Agron Sin, 2014, 40(06): 973-983.
[4] ZHAO Chun-Hua, CUI Fa, LI Jun, DING An-Meng, LI Xin-Feng, GAO Ji-Rong, WANG Hong-Gang. Genetic Difference of Siblines Derived from Winter Wheat Germplasm “Aimengniu” [J]. Acta Agron Sin, 2011, 37(08): 1333-1341.
[5] HE Guang-Hua;PEI Yan;YANG Guang-Wei;XIE Rong. DNA Variance of Parental Lines for Semilate Indica Hybrid Rice in China [J]. Acta Agron Sin, 2000, 26(04): 449-454.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!