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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (4): 679-684.doi: 10.3724/SP.J.1006.2009.00679

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Comparative Study on Genetic Diversity of Wild Rice(Oryza rufipogon Griff.)in China and Three Countries in Southeast Asia

SUN Xi-Ping12,YANG Qing-Wen2*   

  1. 1Shanxi Agricultural University,Taigu 030801,China;2Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2008-08-17 Revised:2008-10-18 Online:2009-04-12 Published:2009-02-16
  • Contact: YANG Qing-Wen E-mail:qwyang@mail.caas.net.cn

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Abstract:

Simple sequence repeat (SSR) analysis was performed to assess the genetic diversity of Oryza rufipogon Griff. using 10 representative populations from China, 2 populations from Vietnam, 1 population from Laos and 2 populations from Cambodia. A total of 282 individuals were screened using 24 different SSR primers. Relationship among populations was analyzed based on the UPGMA cluster method. The results were as follows: (i) Two hundred and eighty nine alleles were detected at 24 microsatellites loci; the average number of alleles (A) was 12; the average effective number of alleles (Ae) was 7; the expected heterozygosity (He) was 0.812; the observed heterozygosity (Ho) was 0.543; the Shannon’ index (I) was 1.99, which meant that the detected populations from 4 countries were rich of genetic diversity. (ii) The populations from China had the most abundant genetic diversity with I of 1.92, while those from Laos, Vietnam and Cambodia were with I of 1.69, 1.47, and 1.45 respectively. (iii) Populations from Laos and Cambodia had the closest relationship, while populations from Vietnam, Laos and Cambodia had the closer relationships to each other than to those from China. (iv) Compared with the other populations in China, these from Wuxuan and Hezhou had the closer relationships to these from Southeast countries.

Key words: Oryza rufipogon Griff., Simple sequent repeat(SSR), Genetic diversity

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