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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (7): 1268-1273.doi: 10.3724/SP.J.1006.2009.01268

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genotype Analysis and QTL Mapping for Tolerance to Low Temperature in Germination by Introgression Lines in Soybean

JIANG Hong-Wei1,2,LI Can-Dong1,2,LIU Chun-Yan1,2,ZHANG Wen-Bo2,QIU Peng-Cheng2,LI Wen-Fu12,GAO Yun-Lai12,HU Guo-Hua13*,CHEN Qing-Shan2*   

  1. 1Land Reclamation Research & Breeding Centre of Heilongjiang,Harbin 150090,China;2College of Agriculture,Northeast Agricultural University,Harbin 150030 China;3 The National Research Center of Soybean Engineering and Technology,Harbin 150050,China
  • Received:2008-10-30 Revised:2009-03-23 Online:2009-07-12 Published:2009-05-19
  • Contact: HU Guo-Hua, E-mail: Hugh757@vip.163.com, Tel: 0451-55199475; CHEN Qing-Shan, E-mail: qshchen@126.com, Tel: 0451-55191945
  • About author:E-mail: j444444@yahoo.cn

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Abstract:

Northeast of China is the main soybean production area. Low temperature is one of the main factors to reduce soybean yield and to limit soybean production spreading to north regions. Shortening the growth stage is considered as one way of solving the problem, but the effective way is to breed cultivars with low temperature to tolerance. The identification of low-temperature tolerance germplasm is the basis for soybean breeding. A set of backcross introgression lines were constructed with Hongfeng 11 as recurrent parent and Clark as donor parent, 46 individuals were screened out, which were more tolerant than recurrent parent Hongfeng 11 under low temperature condition. Fifty individuals from BC1F4 were used as control (random population). Fourteen QTLs on ten linkage groups for tolerance to low temperature in germination were detected with genotype analysis by chi-test and one-way ANOVA. Twelve excessive-introgression alleles from donor parent with positive effect were acquired by chi-square and 5 alleles by ANOVA. Satt237, SOYPRP1, and Satt540 were detected by the two methods, so the three loci should be the main effect QTL and more believable. Introgression lines were used for mapping QTL related with tolerance to low temperature in germination, and the molecular mechanism could be studied in the further research on the platform established in this study for molecular assisted breeding.

Key words: Soybean, Introgression lines, Germination stage, Low temperature tolerance, QTL mapping

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