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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (8): 1516-1524.doi: 10.3724/SP.J.1006.2009.01516


Ultrastructure of Megasprogenesis in Faculative Apomictic Monosonic Addition Line M14  of Beta corolliflora of Sugar Beet

DING Chang-Hong1,SHEN Jia-Heng1,*,GUO De-Dong2,SHANG Ya-Jia1,LU Jun-Ping1   

  1. 1College of Life and Environmental Sciences,Harbin Normal University,Harbin 150025,China;2College of Life Sciences,Heilongjiang University,Harbin 150080,China
  • Received:2009-01-16 Revised:2009-03-15 Online:2009-08-12 Published:2009-06-10
  • Contact: SHEN Jia-Heng, E-mail: hsdshenjiaheng@yahoo.com.cn


Research on ultrastructure of megasporogenesis facultative apomictic monosonic addition line M14 of Beta corolliflora of sugar beet by TEM, in order to get the distinctions between the initial cells of sexual reproduction and apomixes. The embryo-sac development of M14 can be classified into three types: Allium odorum-type, Antennaria-type, and Polygonum-type. There was little difference between Allium odorum-type and Polygonum-type until the stage of dyads. At meiosis I, nuclear vacuoles, synaptonemal complexes showed up, cytoplasmic components underwent differentiation, callose deposited in the wall. The megasporocyte of Allium odorum-type only underwent meiosis I to form a dyad, and then the micropylar dyad cell of Allium odorum-type degenerated quickly, while the chalazal one developed into diploid functional megaspore. In Polygonum-type the micropylar dyad cell degenerated quickly, while the chalazal one finished meiosis II to form two unequal-sized megaspore. The megaspore at chalazal end developed into haploid functional megaspore. Antennaria-type was the only fashion of apomictic recreation of M14. Ultrastructure observation on M14 showed the signs of diploid apomictic recreation: no meiosis, no nuclear vacuoles, no cytoplasmic components differentiation, no callose deposition, and no plasmodesmata.

Key words: Monosomic addition line in sugar beet, Facultative apomixes, Megasporogenesis, Allium odorum-type, Antennaria-type, Polygonum-type, Ultrastructure

[1] Bicknell R A, Koltunow A M. Understanding apomixis: Recent advances and remaining conundrums. Plant Cell, 2004, 16: 228-245

[2] Mu X-J(母锡金), Cai X(蔡雪), Sun D-L(孙德兰), Shi G-C(时光春), Zhu Z-Q(朱至清), Apomixis and its application prospect. Acta Agron Sin (作物学报), 2001, 27(5): 590-599 (in Chinese with English abstract)

[3] Ma H(马虹), Wang Y-F(王燕飞), Cao R(曹瑞). Study on embryology of Haloxylon ammodendron: I. Preliminary observation on microsporogenesis and formation of male gametophytes and apomixis in megasporogenesis. J Desert Res (中国沙漠), 2004, 24(6): 768-772 (in Chinese with English abstract)

[4] Liao F-X(廖飞雄), Li Y-N(李育农). Embryology characteristics and results of apomixis in Malus toringoides (Rehd) Hughus (3x). Acta Agric Univ Jiangxiensis (江西农业大学学报), 1996, 18(3): 287-291(in Chinese with English abstract)

[5] Zang G-G(臧巩固), Zhao L-N(赵立宁), Sun J-S(孙敬三). Cytoembryogical studies on apomixis in Boehmeria silestrll. Acta Bot Sin (植物学报), 1993, 39(3): 210-215 (in Chinese with English abstract)

[6] Carman J G, Crane C F, Riera-Lizarazu O. Comparative histology of cell walls during meiotic and apomeiotic megasporogenesis in two hexaploid Australasian Elymus species. Crop Sci, 1991, 31: 1527-1532

[7] Wu C-H(伍成厚), Li D-M(李冬妹), Liang C-Y(梁承邺), Ye X-L(叶秀麟). Ultrastrural observations on megasporogenesis in Doritis pulcherrima (Orchidaceae). J Trop Subtrop Bot (热带与亚热带植物学报), 2005, 13(1): 45-48 (in Chinese with English abstract)

[8] Li L(李灵), Ji C-G(吉成均), You R-L(尤瑞麟). Ultrastructral studies on megasporogenesis in Triticum aestivum L. J Nat Sci Univ Pekinensis (北京大学学报自然科学版), 2001, 37(4): 443-453 (in Chinese with English abstract)

[9] Jane W N, Chiang S H. Ultrastructure of megasporogenesis and early megasporogenesis in Arundo. Intl J Plant Sci, 1996, 157: 418-431

[10] Ji C-J(吉成均), Yang X(杨雄), Li Z-L(李正理). Ultrastructural studies on megaspore formation in Ginkgo biloba. Acta Bot Sin (植物学报), 1999, 41(12): 1323-1326(in Chinese with English abstract)

[11] Naumova T N, Osadtchiy J V, Sharma V K, Dijkhuis P, Ramulu K S. Apomixis in plants: Structural and functional aspects of diplosopry in Poa menoralis and P. palustris. Protoplasma, 1999, 208: 186-195

[12] Guo D-D(郭德栋), Wang G-Z(王桂芝), Wang J-Z(王继志), Li H-Q(李汉卿). Studies on Beta vulgaris L.×B. corolliflora Zoss interspecific crossing. In: Chinese Genetics Research. Beijing: Chinese Science and Technology Press, 1991. pp 93-94 (in Chinese)

[13] Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍), Kang C-H(康传红), Li Y(李勇), Wang G-Z(王桂芝). Acquisition and identification of monosonic additional line of apomictic beet. J Yunnan Univ (云南大学学报·自然科学版), 1999, 21(S3): 180-181(in Chinese)

[14] Guo D-D(郭德栋), Liu L-P(刘丽萍), Kang C-H(康传红), Li H-Y(李海英), Han X-Y(韩晓云), Tang Y(唐艳), Wang G-Z(王桂芝). The transmission and reproductive characters of apomictic monosomic addition lines of Beta vulgaris L. J Natl Sci Heilongjiang Univ (黑龙江大学学报·自然科学版), 2001, 18(2): 104-107 (in Chinese with English abstract)

[15] Shen Y(申业), Shen J-H(申家恒), Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍). Megasporogenesis and development of female gametophyte in the apomictic monosomic addition line M14 of Beta corolliflora in Sugar Beet. J Wuhan Bot Res (武汉植物学研究), 2006, 24(2): 106-113(in Chinese with English abstract)

[16] Shen Y(申业), Shen J-H(申家恒), Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍). Cyto-embryological study of the apomictic monosomic addition line M14 in sugar beet. Acta Bot Boreali-Occident Sin (西北植物学报), 2006, 26(5): 957-963(in Chinese with English abstract)

[17] Shen Y(申业), Shen J-H(申家恒), Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍). Dynamics of callose deposition in cell wall during megasporogenesis in the apomictic monosomic addition line M14 of Beta corolliflora of sugar beet. Acta Agron Sin (作物学报), 2006, 32(6): 894-898(in Chinese with English abstract)

[18] Dickinson H G. Cytoplasmic differentiation during microsporogenesis in higher plants. Acta Soc Bot Pol, 1981, 50: 3-12

[19] Koltunow A M. Apomixis: Embryo sac and embryos formed without meiosis or fertilization in ovules. Plant Cell, 1993, 5: 1425-1437

[20] Li D-M(李冬妹), Xia K-F(夏快飞), Ye X-L(叶秀璘), Liang C-Y(梁承邺). Ultrastructural observation on megasporogenesis in Phaius tankervilliae (Aiton) Bl. Acta Sci Nat Univ Sunyatseni (中山大学学报自然科学版), 2005, 144(2): 209-212 (in Chinese with English abstract)

[21] Savidan Y, Carman J G, Dresselhaus T. The Flowering of Apomixis: From Mechanisms to Genetic Engineering Mexico, DF: CIMMYT. IRD. European Commission DGVI (FAIR), 2001. pp 44-63

[22] Rodkiewicz B. Callose deposition during megagametogenesis in two species of angiosperms. Planta, 1970, 93: 39-47

[23] Han Y-R(韩贻仁). Molecular Cell Biology. Beijing: Science Press, 2001. pp 174-175 (in Chinese)

[24] Kapil R N, Bhatnagar A K. Ultrastructure and biology of female gametophyte in flowering plants. Intl Rev Cytol, 1981, 70: 291-341

[25] Karasawa R, Ueda K. Nuclear Vacuoles and synthesis during meiotic prophase in Haplopappus gracilis. Cytologia, 1983, 48: 819-826

[26] Vijayaraghavan M R, Bhatia K. Changes during microsporogenesis, vegetative and generative cell formation: A review based on ultrastructure and cytochemistry. Intl Rev Cytol, 1985, 96: 263-296

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