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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (10): 1615-1623.doi: 10.3724/SP.J.1006.2010.01615

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Association Analysis between Submergence Tolerance and SSR Markers in Domestic and Foreign Soybean Cultivars in Asia

SUN  Hui-Min1,ZHANG  Jun1,2,ZHAO  Tuan-Ji1,*,GAI  Jun-Yi1,*   

  1. 1 Soybean Research Institute / Nanjing Agricultural University / National Center for Soybean Improvement / National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China; 2 Binzhou Vocational College, Binzhou 256603, China
  • Received:2010-02-08 Revised:2010-05-23 Online:2010-10-12 Published:2010-08-04

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Abstract: Association mapping is one of the important ways to detect QTLs and reveal genetic bases of target traits in the natural populations. According to the analyses of genetic structure of the total 291 Asian soybean accessions with 64 simple-sequence repeat (SSR) markers, the tested population was divided into Chinese population (Population 1) and exotic population (Population 2). Linkage disequilibrium was detected extensively in both populations. The results of association mapping showed that total 3, 7, and 12 SSR loci in Population 1 were associated with three submergence tolerance traits, i.e. relative seedling death rate, relative chlorophyll deficiency rate and relative wilting rate, while 6, 3, and 5 associated SSR loci were in Population 2. Several loci were associated simultaneously with two or three tolerance traits, indicating their common genetic bases, while some loci were consistent with QTLs detected from family-based linkageship mapping procedure. Total 24 and 22 elite alleles of the three indicators of submergence tolerance, as well as their carriers, were detected in the two populations, respectively. The frequency distribution of elite allele of relative seedling death rate varied among five major pedigrees of released cultivars from Huang-Huai-Hai Valleys and southern China. Some cultivars, such as Hedou 2 Hao, Qiandou 3 Hao, Youbian 31, and Nannong 493-1 in Population 1, and PI208432, PI377576, PI481690 in Population 2, with elite alleles and performance of tolerance to submergence were selected as donors in breeding for tolerance to submergence stress in soybean.

Key words: Released cultivar, Submergence tolerance, SSR marker, Association analysis, Elite allele

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