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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (01): 67-73.doi: 10.3724/SP.J.1006.2011.00067


Primary Infection Suppression of Blumeria graminis f. sp. Tritici and Host Cell Responses Regulated by Pm21 Gene in Wheat

ZHANG Zhen,LIU Xing-Hong,ZHAI Hong-Cui,WANG Hua-Zhong*   

  1. School of Life Sciences / Tianjin Key Laboratory of Cyto-genetical & Molecular Regulation, Tianjin Normal University, Tianjin 300387, China
  • Received:2010-04-28 Revised:2010-07-30 Online:2011-01-12 Published:2010-10-09
  • Contact: WANG Hua-Zhong,E-mail:hsxywhz@mail.tjnu.edu.cn

Abstract: Pm21 is a wide-spectrum resistance gene to powdery mildew (Blumeria graminis f. sp. tritici, Bgt) in wheat (Triticum aestivum L.), which originates from Haynaldia villosa. To reveal the mechanism of Pm21-mediated Bgt resistance in wheat at early infection stage, we carried out the cytological observations using wheat line 92R137, carrying Pm21 gene and presenting immunity (but with visible spots on leaves) to Bgt after artificial inoculation with the prevalent Bgt race 15. Chinese Spring (CS) was used as the susceptible control. In wheat–Bgt interaction sites on leaves, papillae formed at a similar time in challenged epidermal cells in both 92R137 and CS. However, the papillae were larger and more compact and persisted in longer time in 92R137 than in CS, resulting in effectively blocking the penetration of Bgt. Accumulation of H2O2 was observed in the papillae of 92R137 by DAB staining. Appressorium malformation occurred in 92R137, which was probably caused by the failure of multiple penetration attempts of Bgt. For the small number of Bgt that successfully penetrated the epidermal cells of 92R137, haustorium-dependent hypersensitive reactions were induced to suppress further development of haustoria and secondary hyphae, arresting onset of powdery mildew. Therefore, the major mechanism of Pm21-mediated Bgt resistance involves challenged host cell wall reinforcement and hypersensitive reaction.

Key words: Wheat powdery mildew, Pm21 gene, Cell wall reinforcement, Hypersensitive reaction

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