Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 629-634.doi: 10.3724/SP.J.1006.2011.00629

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Gene Mapping of a Novel Mutant ahl in Rice

WANG Zeng**,LI Yun-Feng**,MA Jiao,REN De-Yong,WANG De-Zhong,YOU Xiao-Qing,SANG Xian-Chun,HE Guang-Hua*   

  1. Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China
  • Received:2010-09-17 Revised:2011-01-06 Online:2011-04-12 Published:2011-02-24
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

PDF

2289

Abstract: Theresearch on rice flower development gene is very important in molecular breeding of rice for related traits. A rice flower mutant abnormal hull and lodicule (ahl) was identified from Jinhui 10 (Oryza sativa L. ssp. indica) treated by EMS. In ahl mutant, lemma and palea minished and significantly twisted along with the apparently elongated apical lodicules. This minished lemma and palea led toabnormal kernelsafter grain filling, meanwhile, the 1000-grain weight decreased. The trait wassteadily inherited, and controlled by a pair of recessive gene.Using bulked segregation analysis method AHL was restricted between SSR markers RM14153 and RM14167, with genetic distances of 1.34 cM and 1.19 cM respectively, which covered an approximate 226 kb region on the chromosome 2. The result is useful for further map-based cloning and functional analysis of AHL gene.

Key words: Rice (Oryza sativa L.), abnormal hull and lodicule, Genetic analysis, Gene mapping

[1]Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development. Nature, 1991, 353: 31–37
[2]Weigel D, Meyerowitz E M. The ABCs of floral homeotic genes. Cell, 1994, 78: 203–209
[3]Bowman J L, Drews G N, Meyerowitz E M. Expression of the Arabidopsis floral homeotic gene AGAMOUS is restricted to specific cell-types late in flower development. Plant Cell, 1991, 3: 749–758
[4]Wolfe K H, Gouy M, Yang Y W, Sharp P M, Li W H. Date of the monocot-dicot divergence estimated from chloroplast DNA sequence data. Proc Natl Acad Sci USA, 1989, 86: 6201–6205
[5]Kang H G, Jeon J S, Lee S, An G. Identification of class B and class C floral organ identity genes from rice plants. Plant Mol Biol, 1998, 38: 1021–1029
[6]Lee S, Jeon J S, An K, Moon Y H, Lee S, Chung Y Y, An G. Alteration of floral organ identity in rice through ectopic expression of OsMADS16. Planta, 2003, 217: 904–911
[7]Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, Nagato Y. SUPERWOMAN1, DROOPING LEAF genes control floral organ identity in rice. Development, 2003, 130: 705–718
[8]Xiao H, Wang Y, Liu D F, Wang W M, Li X B, Zhao X F, Xu J C, Zhai W X, Zhu L H. Functional analysis of the rice AP3 homologue OsMADS16 by RNA interference. Plant Mol Biol, 2003, 52: 957−966
[9]Yamaguchi T, Lee D Y, Miyao A, Hirochika H, An G, Hirano H Y. Functional diversification of the two C-class MADS box genes OsMADS3 and OsMADS58 in Oryza sativa. Plant Cell, 2006, 18: 15–28
[10]Pelucchi N, Fornara F, Favalli C, Masiero S, Lago C, Pe M E. Comparative analysis of rice MADS-box genes expressed during flower development. Sex Plant Reprod, 2002, 15: 113–122
[11]Kyozuka J, Kobayashi T, Morita M, Shimamoto K. Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A, B and C genes. Plant Cell Physiol, 2000, 41: 710–718
[12]Greco R, Stagi L, Colombo L, Angenent G C, Sari-Gorla M, Pè ME. MADS-box genes expressed in developing inflorescences of rice and sorghum. Mol Gen Genet, 1997, 253: 615–623
[13]Rogers S O, Bendich AJ. Extraction of DNA from plant tissues. In: Gelvin S B, Schilperoort R A, eds. Plant Molecular Biology Manual. Boston, M A: Kluwer Academic Publishers, 1988. pp A6: 1–10
[14]Sang X-C(桑贤春), He G-H(何光华), Zhang Y(张毅), Yang Z-L(杨正林), Pei Y(裴炎). The simple gain of templates of rice genomes DNA for PCR. Hereditas (遗传), 2003, 25(6): 705–707 (in Chinese with English abstract)
[15]Luo Y-Z(罗远章), Zhao F-M(赵芳明), Sang X-C(桑贤春), Ling Y-H(凌英华), Yang Z-L(杨正林), He G-H(何光华). Genetic analysis and gene mapping of a novel rolled leaf mutant rl12(t) in rice. Acta Agron Sin (作物学报), 2009, 35(11): 1967−1972 (in Chinese with English abstract)
[16]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length poly-morphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 259: 297−607
[17]Kim C, Jeong D H, An G. Molecular cloning and characterization of OsLRK1 encoding a putative receptor-like protein kinase from Oryza sativa. Plant Sci, 2000, 152: 17–26
[18]Agrawal G K, Abe K, Yamazaki M, Miyao A, Hirochika H. Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss-of-function mutants of the OsMADS1 gene. Plant Mol Biol, 2005, 59: 125–135
[19]Yuan Z, Gao S, Xue D W, Luo D, Li L T, Ding S Y, Yao X, Wilson Z A, Qian Q, Zhang D B. RETARDED PALEA1 controls palea development and floral zygomorphy in rice. Plant Physiol, 2009, 149: 235–244
[20]Luo Q, Zhou K D, Zhao X F, Zeng Q C, Xia H G, Zhai W X, Xu J C, Wu X J, Yang H S, Zhu L H. Identification and fine mapping of a mutant gene for palealess spikelet in rice. Planta, 2005, 221: 222–230
[21]Ohmori S, Kimizu M, Sugita M, Miyao A, Hirochika H, Uchida E, Nagato Y, Yoshida H. MOSAIC FLORAL ORGANS1, an AGL6-Like MADS Box Gene, Regulates floral organ identity and meristem fate in rice. Plant Cell, 2009, 21: 3008–3025
[22]Xiao H, Tang J F, Li Y F, Wang W M, Li X B, Jin L, Xie R, Luo H F, Zhao X F, Meng Z, He G H, Zhu L H. STAMENLESS 1, encoding a single C2H2 zinc finger protein, regulates floral organ identity in rice. Plant J, 2009, 59: 789–801
[23]Kyozuka J, Kobayashi T, Morita M, Shimamoto K. Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A, B and C genes. Plant Cell Physiol, 2000, 41: 710–718
[24]Moon S, Jung K H, Lee D E, Lee DY, Lee J, An K, Kang H G, An G. The rice FON1 gene controls vegetative and reproductive development by regulating shoot apical meristem size. Mol Cells, 2006, 21: 147–152
[25]Prasad K, Vijayraghavan U. Double-stranded RNA interference of a rice PI/GLO paralog, OsMADS2, uncovers its second-whorl-specific function in floral organ patterning. Genetics, 2003, 165: 2301–2305
[26]Franks R G, Liu Z C, Fischer R L. SEUSS and LEUNIG regulate cell proliferation, vascular development and organ polarity in Arabidopsis petals. Planta, 2006, 224: 801–811
[1] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[2] WANG Hao-Rang, ZHANG Yong, YU Chun-Miao, DONG Quan-Zhong, LI Wei-Wei, HU Kai-Feng, ZHANG Ming-Ming, XUE Hong, YANG Meng-Ping, SONG Ji-Ling, WANG Lei, YANG Xing-Yong, QIU Li-Juan. Fine mapping of yellow-green leaf gene (ygl2) in soybean (Glycine max L.) [J]. Acta Agronomica Sinica, 2022, 48(4): 791-800.
[3] LIU Lei, ZHAN Wei-Min, DING Wu-Si, LIU Tong, CUI Lian-Hua, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping. Genetic analysis and molecular characterization of dwarf mutant gad39 in maize [J]. Acta Agronomica Sinica, 2022, 48(4): 886-895.
[4] XU Ning-Kun, LI Bing, CHEN Xiao-Yan, WEI Ya-Kang, LIU Zi-Long, XUE Yong-Kang, CHEN Hong-Yu, WANG Gui-Feng. Genetic analysis and molecular characterization of a novel maize Bt2 gene mutant [J]. Acta Agronomica Sinica, 2022, 48(3): 572-579.
[5] ZHAO Mei-Cheng, DIAO Xian-Min. Phylogeny of wild Setaria species and their utilization in foxtail millet breeding [J]. Acta Agronomica Sinica, 2022, 48(2): 267-279.
[6] JIANG Jian-Hua, ZHANG Wu-Han, DANG Xiao-Jing, RONG Hui, YE Qin, HU Chang-Min, ZHANG Ying, HE Qiang, WANG De-Zheng. Genetic analysis of stigma traits with genic male sterile line by mixture model of major gene plus polygene in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2021, 47(7): 1215-1227.
[7] YIN Ming, YANG Da-Wei, TANG Hui-Juan, PAN Gen, LI De-Fang, ZHAO Li-Ning, HUANG Si-Qi. Genome-wide identification of GRAS transcription factor and expression analysis in response to cadmium stresses in hemp (Cannabis sativa L.) [J]. Acta Agronomica Sinica, 2021, 47(6): 1054-1069.
[8] HUANG Xing, XI Jin-Gen, CHEN Tao, QIN Xu, TAN Shi-Bei, CHEN He-Long, YI Ke-Xian. Identification and expression of PAL genes in sisal [J]. Acta Agronomica Sinica, 2021, 47(6): 1082-1089.
[9] WU Ran-Ran, LIN Yun, CHEN Jing-Bin, XUE Chen-Chen, YUAN Xing-Xing, YAN Qiang, GAO Ying, LI Ling-Hui, ZHANG Qin-Xue, CHEN Xin. Genetic and cytological analysis of male sterile mutant msm2015-1 in mungbean [J]. Acta Agronomica Sinica, 2021, 47(5): 860-868.
[10] JIANG Cheng-Gong, SHI Hui-Min, WANG Hong-Wu, LI Kun, HUANG Chang-Ling, LIU Zhi-Fang, WU Yu-Jin, LI Shu-Qiang, HU Xiao-Jiao, MA Qing. Phenotype analysis and gene mapping of small kernel 7 (smk7) mutant in maize [J]. Acta Agronomica Sinica, 2021, 47(2): 285-293.
[11] GUO Qing-Qing, ZHOU Rong, CHEN Xue, CHEN Lei, LI Jia-Na, WANG Rui. Location and InDel markers for candidate interval of the orange petal gene in Brassica napus L. by next generation sequencing [J]. Acta Agronomica Sinica, 2021, 47(11): 2163-2172.
[12] HUANG Yan, HE Huan-Huan, XIE Zhi-Yao, LI Dan-Ying, ZHAO Chao-Yue, WU Xin, HUANG Fu-Deng, CHENG Fang-Min, PAN Gang. Physiological characters and gene mapping of a dwarf and wide-leaf mutant osdwl1 in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2021, 47(1): 50-60.
[13] JIANG Hong-Rui, YE Ya-Feng, HE Dan, REN Yan, YANG Yang, XIE Jian, CHENG Wei-Min, TAO Liang-Zhi, ZHOU Li-Bin, WU Yue-Jin, LIU Bin-Mei. Identification and gene localization of a novel rice brittle culm mutant bc17 [J]. Acta Agronomica Sinica, 2021, 47(1): 71-79.
[14] SHI Hui-Min, JIANG Cheng-Gong, WANG Hong-Wu, MA Qing, LI Kun, LIU Zhi-Fang, WU Yu-Jin, LI Shu-Qiang, HU Xiao-Jiao, HUANG Chang-Ling. Phenotype identification and gene mapping of defective kernel 48 mutant (dek48) in maize [J]. Acta Agronomica Sinica, 2020, 46(9): 1359-1367.
[15] ZHANG Xue-Cui,ZHONG Chao,DUAN Can-Xing,SUN Su-Li,ZHU Zhen-Dong. Fine mapping of Phytophthora resistance gene RpsZheng in soybean cultivar Zheng 97196 [J]. Acta Agronomica Sinica, 2020, 46(7): 997-1005.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd