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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (11): 1975-1983.doi: 10.3724/SP.J.1006.2011.01975

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Establishment of Molecular Identity in 142 Sweet Sorghum Varieties

WANG Li-Ming,JIAO Shao-Jie,JIANG Yan-Xi,YAN Hong-Dong,SU De-Feng,SUN Guang-Quan   

  1. Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
  • Received:2011-05-25 Revised:2011-07-15 Online:2011-11-12 Published:2011-09-06

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Abstract: A large change occurs in phenotypic characters under different environments for introducing and frequently exchanging of abundant varieties, and accurate identification is usually affected by the similar phenotypic characters performed in the field, so it is necessary to establish ID to distinguish varieties between each other. A total of 103 SSR primers covering 10 linkage groups of sorghum chromosome were screened, 41 primers showing polymorphic bands were used to establish molecular ID of 142 sweet sorghum germplasm resources. A total of 189 polymorphic bands were amplified and obtained. The number of alleles revealed by each primer was 2-11 with an average of 4.6 per primer. The polymorphism information content (PIC) value for the SSR loci ranged from 0.089 to 0.850 with an average of 0.543. There existed much difference in specific indices of varieties, ranging from 109.1 to 454.7 with an average of 189.0. The results showed that 11 primers (Xtxp329, Xtxp258, Xtxp113, Xtxp303, Xtxp61, Xtxp201, Xtxp141, Xtxp91, Xtxp47, Xtxp217, and Xtxp67) were confirmed on the basis of allele number of each pair of primers. The IDs of 142 sweet sorghum germplasm resources were established by the 11 pairs of primers, which can discriminate a variety from others.

Key words: Sweet sorghum, Germplasm resources, Molecular identity

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