Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (11): 1975-1983.doi: 10.3724/SP.J.1006.2011.01975

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Establishment of Molecular Identity in 142 Sweet Sorghum Varieties

WANG Li-Ming,JIAO Shao-Jie,JIANG Yan-Xi,YAN Hong-Dong,SU De-Feng,SUN Guang-Quan   

  1. Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
  • Received:2011-05-25 Revised:2011-07-15 Online:2011-11-12 Published:2011-09-06

PDF

1276

Cited

26

Abstract: A large change occurs in phenotypic characters under different environments for introducing and frequently exchanging of abundant varieties, and accurate identification is usually affected by the similar phenotypic characters performed in the field, so it is necessary to establish ID to distinguish varieties between each other. A total of 103 SSR primers covering 10 linkage groups of sorghum chromosome were screened, 41 primers showing polymorphic bands were used to establish molecular ID of 142 sweet sorghum germplasm resources. A total of 189 polymorphic bands were amplified and obtained. The number of alleles revealed by each primer was 2-11 with an average of 4.6 per primer. The polymorphism information content (PIC) value for the SSR loci ranged from 0.089 to 0.850 with an average of 0.543. There existed much difference in specific indices of varieties, ranging from 109.1 to 454.7 with an average of 189.0. The results showed that 11 primers (Xtxp329, Xtxp258, Xtxp113, Xtxp303, Xtxp61, Xtxp201, Xtxp141, Xtxp91, Xtxp47, Xtxp217, and Xtxp67) were confirmed on the basis of allele number of each pair of primers. The IDs of 142 sweet sorghum germplasm resources were established by the 11 pairs of primers, which can discriminate a variety from others.

Key words: Sweet sorghum, Germplasm resources, Molecular identity

[1]Liu G-S(刘公社), Zhou Q-Y(周庆源), Song S-Q(宋松泉), Jing H-C(景海春), Gu W-B(谷卫彬), Li X-F(李晓峰), Su M(苏蔓), Ramachandran Srinivasan1. Research advances into germplasm resources and molecular biology of the energy plant sweet sorghum. Chin Bull Bot (植物学报), 2009, 44(3): 253–261 (in Chinese with English abstract)
[2]Wu W(武文), Deng Q-Y(邓启云), Zhou L-J(周丽洁), Zhu X-Q(朱校奇), Chen C-G(陈春光), Zhuang W(庄文), Jin D-M(金德敏), Wang B(王斌). Establishment of DNA fingerprinting with SSR for Y58S and some other elite parents of two-line hybrid rice. Hybrid Rice (杂交水稻), 2008, 23(3): 52–56 (in Chinese with English abstract)
[3]Gao Y-L(高运来), Zhu R-S(朱荣胜), Liu C-Y(刘春燕), Li W-F(李文福), Jiang H-W(蒋洪蔚), Li C-D(李灿东), Yao B-C(姚丙晨), Hu G-H(胡国华), Chen Q-S(陈庆山). Establishment of molecular ID in soybean varieties in Heilongjiang, China. Acta Agron Sin (作物学报), 2009, 35(2): 211–218 (in Chinese with English abstract)
[4]Zhao H-K(赵洪锟), Li Q-Y(李启云), Wang Y-M(王玉民), Zhang M(张明), Zhuang B-C(庄炳昌). DNA fingerprint of the main parents and current varieties of soybean in Jilin province. Chin J Oil Crop Sci (中国油料作物学报), 2000, 22(4): 12–16 (in Chinese with English abstract)
[5]Wang L-X(王立新), Li Y-F(李云伏), Chang L-F(常利芳), Huang L(黄岚), Li H-B(李宏博), Ge L-L(葛玲玲), Liu L-H(刘丽华), Yao J(姚骥), Zhao C-P(赵昌平). Method of ID constitution for wheat cultivars. Acta Agron Sin (作物学报), 2007, 33(10): 1738–1740 (in Chinese with English abstract)
[6]Li X-H(李晓辉), Li X-H(李新海), Gao W-W(高文伟), Tian Q-Z(田清震), Li M-S(李明顺), Ma F-M(马凤鸣), Zhang S-H(张世煌). Establishment of DNA fingerprinting database of maize hybrids and its application in parentage identification. Acta Agron Sin (作物学报), 2005, 31(3): 386–391 (in Chinese with English abstract)
[7]Wang F-G(王凤格), Zhao J-R(赵久然), Guo J-L(郭景伦), Liu L-Z(刘龙洲). Series of research on establishing DNA finger-printing poll of Chinese new maize cultivars: I. The establishment of a standard SSR system fitting for maize cultivars identification. J Maize Sci (玉米科学), 2003, 11(1): 3–6 (in Chinese with English abstract)
[8]Liu P-W(刘平武), Zhou G-L(周国岭), Yang G-S(杨光圣), Fu T-D(傅廷栋). Fingerprints construction of hybrid parents in Brassica napus and its utilization in hybrid purity test. Acta Agron Sin (作物学报), 2005, 31(5): 640–646 (in Chinese with English abstract)
[9]Qi L(齐兰), Wang W-Q(王文泉), Zhang Z-W(张振文), Ye J-Q(叶剑秋), Li K-M(李开绵). DNA Fingerprinting analysis of 18 cassava varieties using sequence-related amplified polymorphism markers. Acta Agron Sin (作物学报), 2010, 36(10): 1642–1648 (in Chinese with English abstract)
[10]Fu Y-H(付瑜华), Li J(李杰), Wang H-Y(王海燕), Yang Z-X(杨子贤), Wang W-Q(王文泉). Establishment of fingerprints for several commercial cultivars in cassava. J Plant Genet Resour (植物遗传资源学报), 2007, 8(1): 51–55 (in Chinese with English abstract)
[11]Piperidis G, Rattey A R, Taylor G O, Cox M C. DNA markers: a tool for identifying sugarcane varieties. Austral Sugarcane, 2004, 8(suppl): 1–8
[12]Zheng H-Y(郑海燕), Su J-G(粟建光), Dai Z-G(戴志刚), Li Y(李燕), Chen J-Q(陈基权), Gong Y-C(龚友才). Establishment of molecular identity for kenaf germplasm using ISSR and RAPD markers. Sci Agric Sin (中国农业科学), 2010, 43(17): 3499–3510 (in Chinese with English abstract)
[13]Liu X-L(刘新龙), Ma L(马丽), Chen X-K(陈学宽), Ying X-M(应雄美), Cai Q(蔡青), Liu J-Y(刘家勇), Wu C-W(吴才文). Establishment of DNA fingerprint ID in sugarcane cultivars in Yunnan, China. Acta Agron Sin (作物学报), 2010, 36(2): 202–210 (in Chinese with English abstract)
[14]Ali M L, Rajewski J F, Baenziger P S, Gill K S, Eskridge K M, Dweikat I. Assessment of genetic diversity and relationship among a collection of US sweet sorghum germplasm by SSR markers. Mol Breed, 2008, 21: 497–509
[15]Zhao X-N(赵香娜), Yue M-Q(岳美琪), Liu Y(刘洋), Dun B-Q(顿宝庆), Zhao W-H(赵伟华), Tan L(谭亮), Li G-Y(李桂英). Genetic diversity of domestic and foreign sweet sorghum germplasm revealed by SSR markers. J Plant Genet Resour (植物遗传资源学报), 2010, 11(4): 407–412 (in Chinese with English abstract)
[16]Fang X-E(方雪恩), Chen Q(陈沁), Yin L-P(印丽萍), Wang W(王伟). Application of ISSR in genetic relationship analysis of sorghum species. Acta Agron Sin (作物学报), 2008, 34(8): 1480–1483
[17]Chen Q-S(陈庆山). Comparative analysis of specialty index and genetic similarity on soybean germplasm with resistance to Cercospora sojina Hara. China Biotechnol (中国生物工程杂志), 2005, 25(suppl): 155–158 (in Chinese with English abstract)
[18]Yuan L-X(袁力行), Fu J-H(傅骏骅), Warburton M, Li X-H(李新海), Zhang S-H(张世煌), Khairallah M, Liu X-Z(刘新芝), Peng Z-B(彭泽斌), Li L-C(李连城). Compare of genetic diversity among maize inbred lines based on RFLPs, SSRs, AFLPs and RAPDs. Acta Genet Sin (遗传学报), 2000, 27(8): 725–733 (in Chinese with English abstract)
[19]Guo W-Z(郭旺珍), Zhang T-Z(张天真), Pan J-J(潘家驹), He J-L(何金龙). Analysis of RAPD finger printing on main cotton cultivars in China. J Agric Biotechnol (农业生物技术学报), 1996, 4(2): 129–134 (in Chinese with English abstract)
[20]Russell J R, Fuller J D, Macaulau M, Hatz B G, Jahoor A, Powel W, Waugh R. Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theor Appl Genet, 1997, 9: 714–722
[21]Zhang H(张晗), Wang J-C(王建成), Wang D-J(王东建), Yao F-X(姚凤霞), Xu J-F(许金芳), Song G-A(宋国安), Guan Y-A(管延安), Li R-Y(李汝玉). Assessment of genetic diversity in Chinese sorghum landraces using SSR markers as compared with foreign accessions. Acta Agron Sin (作物学报), 2011, 37(2): 224–234 (in Chinese with English abstract)
[1] CHEN Xiao-Hong, LIN Yuan-Xiang, WANG Qian, DING Min, WANG Hai-Gang, CHEN Ling, GAO Zhi-Jun, WANG Rui-Yun, QIAO Zhi-Jun. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR [J]. Acta Agronomica Sinica, 2022, 48(4): 908-919.
[2] HU Liang-Liang, WANG Su-Hua, WANG Li-Xia, CHENG Xu-Zhen, CHEN Hong-Lin. Identification of salt tolerance and screening of salt tolerant germplasm of mungbean (Vigna radiate L.) at seedling stage [J]. Acta Agronomica Sinica, 2022, 48(2): 367-379.
[3] ZHANG He, JIANG Chun-Ji, YIN Dong-Mei, DONG Jia-Le, REN Jing-Yao, ZHAO Xin-Hua, ZHONG Chao, WANG Xiao-Guang, YU Hai-Qiu. Establishment of comprehensive evaluation system for cold tolerance and screening of cold-tolerance germplasm in peanut [J]. Acta Agronomica Sinica, 2021, 47(9): 1753-1767.
[4] WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agronomica Sinica, 2021, 47(7): 1259-1274.
[5] SUN Zhi-Guang, WANG Bao-Xiang, ZHOU Zhen-Ling, FANG Lei, CHI Ming, LI Jing-Fang, LIU Jin-Bo, Bello Babatunde Kazeem, XU Da-Yong. Screening of germplasm resources and QTL mapping for germinability under submerged condition in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2021, 47(1): 61-70.
[6] ZHANG Xiao-Jun,XIAO Jin,WANG Hai-Yan,QIAO Lin-Yi,LI Xin,GUO Hui-juan,CHANG Li-Fang,ZHANG Shu-Wei,YAN Xiao-Tao,CHANG Zhi-Jian,WU Zong-Xin. Evaluation of resistance to Fusarium head blight in Thinopyrum-derived wheat lines [J]. Acta Agronomica Sinica, 2020, 46(01): 62-73.
[7] GAO Huan-Huan,YE Sang,WANG Qian,WANG Liu-Yan,WANG Rui-Li,CHEN Liu-Yi,TANG Zhang-Lin,LI Jia-Na,ZHOU Qing-Yuan,CUI Cui. Screening and comprehensive evaluation of aluminum-toxicity tolerance during seed germination in Brassca napus [J]. Acta Agronomica Sinica, 2019, 45(9): 1416-1430.
[8] Cui CUI,Chuang CHENG,Yu-Feng ZHAO,Huan-Huan GAO,Rui-Li WANG,Liu-Yan WANG,Qing-Yuan ZHOU. Screening and comprehensive evaluation of aluminum-toxicity tolerance during germination stage in 52 varieties (lines) of pea germplasm [J]. Acta Agronomica Sinica, 2019, 45(5): 798-805.
[9] YUAN Yi-Chuan, CHEN Xiao-Yu, LI Ming-Ming, LI Ping, JIA Ya-Tao, HAN Yuan-Huai, XING Guo-Fang. Screening of germplasm tolerant to low phosphorus of seedling stage and response of root protective enzymes to low phosphorus in foxtail millet [J]. Acta Agronomica Sinica, 2019, 45(4): 601-612.
[10] Qian WANG,Cui CUI,Sang YE,Ming-Sheng CUI,Yu-Feng ZHAO,Na LIN,Zhang-Lin TANG,Jia-Na LI,Qing-Yuan ZHOU. Screening and Comprehensive Evaluation of Germplasm Resources with Tribenuron-methyl Tolerance at Germination Stage in Rapeseed (Brassica napus L.) [J]. Acta Agronomica Sinica, 2018, 44(8): 1169-1184.
[11] Guang-Long ZHU,Cheng-Yu SONG,Lin-Lin YU,Xu-Bing CHEN,Wen-Fang ZHI,Jia-Wei LIU,Xiu-Rong JIAO,Gui-Sheng ZHOU. Alleviation Effects of Exogenous Growth Regulators on Seed Germination of Sweet Sorghum under Salt Stress and Its Physiological Basis [J]. Acta Agronomica Sinica, 2018, 44(11): 1713-1724.
[12] CHEN Li-Na,SI Hai-Ping,FANG Wei,CHEN Yan-Qing,CAO Yong-Sheng. Visualization Study for Investigation Data of Crop Germplasm Resources in Guizhou Province [J]. Acta Agron Sin, 2017, 43(09): 1300-1307.
[13] CHEN Yan-Qing,CAO Yong-Sheng,FANG Wei*,CHEN Li-Na. Spatial Distribution Characteristics of National Crop Germplasm Resources Based on Spatial Statistics in Comprehensive Agricultural Regional Scale [J]. Acta Agron Sin, 2017, 43(03): 378-388.
[14] CHEN Li-Na,FANG Wei,SI Hai-Ping,CAO Yong-Sheng. Ontology Construction of Crop Germplasm Resources [J]. Acta Agron Sin, 2016, 42(03): 407-414.
[15] TANG Chao-Chen,LUO Feng,LI Xin-Yu,PEI Zhong-You,GAO Jian-Ming,SUN Shou-Jun*. Responsiveness of Sweet Sorghum in Yield and Quality Related Traits to Environmental Factors [J]. Acta Agron Sin, 2015, 41(10): 1612-1618.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd