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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 808-813.doi: 10.3724/SP.J.1006.2012.00808


Cloning and Analysis on Full-length cDNA Sequence of Ubiquitin-conjugating Enzyme Gene from Oryza meyeriana Baill.

JIANG Chun-Miao1,2,HUANG Xing-Qi1,FU Jian1,YU Teng-Qiong1,ZHONG Qiao-Fang1,LI Ding-Qin1,YIN Fu-You1,ZHANG Dun-Yu1,WANG Ling-Xian1,CHENG Zai-Quan1,*   

  1. 1 Biotechnology & Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China; 2 College of Life Science, Yunnan University, Kunming 650091, China
  • Received:2011-10-19 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 程在全, E-mail: czquan-99@163.com, Tel: 13888556318, 0871-5140200

Abstract: Based on the bioinformatic analysis of the ESTs from the subtraction cDNA library of Oryza meyeriana, we cloned one of the ESTs which has the homolog function of ubiquitin-conjugating enzyme and got a full-length cDNA sequence of ubiquitin-conjugating enzyme gene by RACE, and named it OmE2 (Oryza meyerianaBaill ubiquitin-conjugating enzyme, OmE2). This full-length cDNA sequence has 917 bp including 528 bp open reading frame encoding a protein of 175 amino acids, with a predicted molecular mass of 19.3 kD and pI of 9.32. OmE2 protein which had significant amino sequence identity and similarity with ubiquitin-conjugating enzyme from other species contained the conserved sequence and cysteine residue in active sites of ubiquitin-conjugating family. OmE2 had three trans-membrane domains. It is predicted that OmE2 protein is a kind of ubiquitin-conjugating protein and transmembrane protein. The expression of OmE2 inOryza meyeriana was induced by Xanthomonas campestris pv. oryzae. Therefore OmE2 gene which was first found in wild rice may participate in the signal transduction and the resistance response in Oryza meyeriana.

Key words: Oryza meyeriana Baill., Ubiquitin-conjugating enzyme, Sequence analysis

[1]Dong F-C(董发才), Song C-P(宋纯鹏). The ubiquitin and physiological functions of plant cell. Plant Physiol Commun (植物生理学通讯), 1999, 35(1): 54–59 (in Chinese)

[2]Ingvardsen C, Veierskov B. Ubiquitin and proteasome-dependent proteolysis in plant. Physiol Plant, 2001, 112: 451–459

[3]Smalle J, Viertra R D. The ubiquitin 26S proteasome proteolytic pathway. Annu Rev Plant Biol, 2004, 16: 3181–3195

[4]Dreher K, Callis J. Ubiquitin, hormones and biotic stress in plants. Ann Bot, 2007, 99: 787–822

[5]Wang J-L(王金利), Shi S-Q(史胜青), Jia L-Q(贾利强), Jiang Z-P(江泽平). Progress on functions of ubiquitin-conjugating enzyme (E2) in plants. Biotechnol Bull (生物技术通报), 2010, (4): 7–9 (in Chinese with English abstract)

[6]Pickart C M. Mechanisms underlying ubiquitination. Annu Revbiochem, 2001, 70: 503–533

[7]Lau O S, Deng X W. Effect of Arabidopsis COP10 ubiquitin E2 enhancement activity across E2 families and functional conservation among its canonical homologs. Biochem J, 2009, 418: 683–690

[8]Lin W Y, Lin S I, Chiou T I. Molecular regulators of phosphate homeostasis in plants. J Exp Bot, 2009, 60: 1427–1438

[9]Feussner K, Feussner I, Leopold I. Isolation of a cDNA coding for an uniquitin-conjugating enzyme UBC1 of tomato—the first stress-induced UBC of higher plants. FEBS Lett, 1997, 409: 211–215

[10]Xu C-X(徐晨曦), Jiang J(姜静), Liu T-T(刘甜甜), Wang Y-C(王玉成), Liu G-F(刘桂丰), Yang C-P(杨传平). Sequence analysis and function determination of E2s gene from Tamarix androssowii. Northeast For Univ (东北林业大学学报), 2007, 35(11): 1–4 (in Chinese with English abstract)

[11]Lu Y-S(陆月赏), Liu Y-H(刘颖慧), Zhang D-F(张登峰), Shi Y-S(石云素), Song Y-C(宋燕春), Wang T-Y(王天宇), Yang D-G(杨德光), Li Y(黎裕). Identification and expression analysis of ZmERD16: a ubiquitin extension protein gene in maize (Zea mays L.). Acta Agron Sin (作物学报), 2010, 36(7): 1075–1083 (in Chinese with English abstract)

[12]Yi L-F(易乐飞), Liu C-W(刘楚吾), Wang P(王萍), Zhou X-H(周向红). cDNA cloning and characterization of a novel ubiquitin-conjugating enzyme gene from Porphyra yezoensis Ueda. J Fish China (水产学报), 2009, 33(5): 719–725 (in Chinese with English abstract)

[13]Guo Q-F(郭启芳). Cloning and Transformation of Ubiquitin Gene in Winter Wheat and Its Expression. MS Dissertation of Shandong Agricultural University, 2004 (in Chinese with English abstract)

[14]Yan H-J(宴慧君). Construction and Analysis of Oryza meyeriana Suppression Subtractive Hybridization Library under Xanthomonas campestris pv. oryza Stress. MS Dissertation of Yunnan University, 2007 (in Chinese with English abstract)

[15]Xu L, Menard R, Berr A. The E2 ubiquitin-conjugation enzymes, AtUBC1 and AtUBC2, play redundant role and are involved in activation of FLC expression and repression of flowering in Arabidopsis thaliana. Plant J, 2009, 57: 279–288

[16]Song J-H(宋江华), Zhang L-X(张立新). Progress on the transmembrane protein in plants. J Biol (生物学杂志), 2009, 26(6): 62–64 (in Chinese with English abstract)

[17]Chen X F, Wang B Y, Wu R. A gibberellin-stimulated ubiquitin-conjugating enzyme gene is involved in ?-amylase gene expression in rice aleurone. Plant Mol Biol, 1995, 29: 787–795
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