Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 814-819.doi: 10.3724/SP.J.1006.2012.00814

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Effect of Successive Backcrossing on Eliminating Somaclonal Variation Caused by Agrobacterium-Mediated Transformation in Rice

YANG Zhou1,2,CHEN Hao1,TANG Wei3,LIN Yong-Jun1,*   

  1. 1 National Key Laboratory of Crop Genetic Improvement / National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan
    430070, China; 2 Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China; 3 Biochemistry Department, Hubei
    University of Medicine, Shiyan 442000, China
  • Received:2011-08-29 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 林拥军, E-mail: yongjunlin@mail.hzau.edu.cn

PDF

1617

Cited

4

Abstract: Somaclonal variation caused by Agrobacterium-mediated transformation influences some agronomic traits of transgenic plants. As a result, a large number of T0 regenerated plants are required in the development of transgenic crop. Here, we successively backcrossed transgenic rice (Oryza sativa L.) to progenitor variety and evaluated agronomic performance of progeny, with the objective of eliminating somaclonal variation and recovering agronomic traits of transgenic parent. Three transgenic lines possessing different insect-resistance genes from Bacillus thuringiensis (Bt) were used as donor parents. They were backcrossed successively to the progenitor variety, and the individuals of each BCnF1 generation were then selfed twice, with selection for insect-resistance. BCnF3 homozygous lines, which were selected by a germination assay, caused an insect mortality of 100% in laboratory bioassay. In the field evaluation, these lines showed obviously higher yield than the donor parents and no significant differences from the progenitor variety in most agronomic traits. These results indicate that successive backcrossing can to a large extent recover agronomic traits of transgenic rice and reduce the workload required in transgenic breeding program.

Key words: Successive backcross, Somaclonal variation, Agrobacterium-mediated transformation, Transgenic rice, Insect-resistance, Agronomic trait

[1]Hiei Y, Komari T, Kubo T. Transformation of rice mediated by Agrobacterium tumefaciens. Plant Mol Biol, 1997, 35: 205–218

[2]Lin Y J, Zhang Q F. Optimising the tissue culture conditions for high efficiency transformation of indica rice. Plant Cell Rep, 2005, 23: 540–547

[3]Shu Q Y, Cui H R, Ye G Y, Wu D X, Xia Y W, Gao M W, Altosaar I. Agronomic and morphological characterization of Agrobacterium-transformed Bt rice plants. Euphytica, 2002, 127: 345–352

[4]Dale P J, McPartlan H C. Field performance of transgenic potato plants compared with controls regenerated from tuber discs and shoot cuttings. Theor Appl Genet, 1992, 84: 585–591

[5]Wang G J, Castiglione S, Chen Y, Li L, Han Y F, Tian Y C, Gabriel D W, Han Y N, Mang K Q, Sala F. Poplar (Populus nigra L.) plants transformed with a Bacillus thuringiensis toxin gene: insecticidal activity and genomic analysis. Transgenic Res, 1996, 5: 289–301

[6]Feldmann K A, Marks M D, Christianson M L, Quatrano R S. A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis. Science, 1989, 243: 1351-1354

[7]Bregitzer P, Halbert S E, Lemaux P G. Somaclonal variation in the progeny of transgenic barley. Theor Appl Genet, 1998, 96: 421–425

[8]Arencibia A D, Carmona E R, Cornide M T, Castiglione S, O’Relly J, Chinea A, Oramas P, Sala F. Somaclonal variation in insect-resistant transgenic sugarcane (Saccharum hybrid) plants produced by cell electroporation. Transgenic Res, 1999, 8: 349–360

[9]Hiei Y, Ohta S, Komari T, Kumashiro T. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J, 1994, 6: 271–282

[10]Labra M, Savini C, Bracale M, Pelucchi N, Colombo L, Bardini M, Sala F. Genomic changes in transgenic rice (Oryza sativa L.) plants produced by infecting calli with Agrobacterium tumefaciens. Plant Cell Rep, 2001, 20: 325–330

[11]Larkin P J, Scowcroft W R. Somaclonal variation—a novel source of variability from cell cultures for plant improvement. Theor Appl Genet, 1981, 60: 197–214

[12]Bertin P, Bouharmont J. Use of somaclonal variation and in vitro selection for chilling tolerance improvement in rice. Euphytica, 1997, 96: 135–142

[13]Van Sint Jan V, de Macedo C C, Kinet J M, Bouharmont J. Selection of Al-resistant plants from a sensitive rice cultivar, using somaclonal variation, in vitro and hydroponic cultures. Euphytica, 1997, 97: 303–310

[14]Sundaram R M, Vishnupriya M R, Biradar S K, Laha G S, Reddy G N, Rani N S, Sarma N P, Sonti R V. Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety. Euphytica, 2008, 160: 411–422

[15]Sundaram R M, Vishnupriya M R, Laha G S, Rani N S, Rao P S, Balachandran S M, Reddy G N, Sarma N P, Sonti R V. Introduction of bacterial blight resistance into Triguna, a high yielding, mid-early duration rice variety. Biotechnol J, 2009, 4: 400–407

[16]Ragagnin V A, de Souza T L P O, Sanglard D A, Arruda K M A, Costa M R, Alzate-Marin A L, Carneiro J E, Moreira M A, de Barros E G. Development and agronomic performance of common bean lines simultaneously resistant to anthracnose, angular leaf spot and rust. Plant Breed, 2009, 128: 156–163

[17]Micallef M C, Austin S, Bingham E T. Improvement of transgenic alfalfa by backcrossing. In Vitro Cell Dev Biol Plant, 1995, 31: 187–192

[18]Bregitzer P, Dahleen L S, Neate S, Schwarz P, Manoharan M. A single backcross effectively eliminates agronomic and quality alterations caused by somaclonal variation in transgenic barley. Crop Sci, 2008, 48: 471–479

[19]Chen H, Tang W, Xu C G, Li X H, Lin Y J, Zhang Q F. Transgenic indica rice plants harboring a synthetic cry2A* gene of Bacillus thuringiensis exhibit enhanced resistance against lepidopteran rice pests. Theor Appl Genet, 2005, 111: 1330–1337

[20]Tang W, Chen H, Xu C G, Li X H, Lin Y J, Zhang Q F. Development of insect-resistant transgenic indica rice with a synthetic cry1C* gene. Mol Breed, 2006, 18: 1–10

[21]Chen H, Zhang G A, Zhang Q F, Lin Y J. Effect of transgenic Bacillus thuringiensis rice lines on mortality and feeding behavior of rice stem borers (Lepidoptera: Crambidae). J Econ Entomol, 2008, 101: 182–189

[22]Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 1962, 15: 473–497

[23]Thompson C J, Movva N R, Tizard R, Crameri R, Davies J E, Lauwereys M, Botterman J. Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO J, 1987, 6: 2519–2523

[24]Kaeppler S M, Kaeppler H F, Rhee Y. Epigenetic aspects of somaclonal variation in plants. Plant Mol Biol, 2000, 43: 179–188

[25]Bergelson J, Purrington C B, Palm C J, López-Gutiérrez J C. Costs of resistance: a test using transgenic Arabidopsis thaliana. Proc Biol Sci, 1996, 263: 1659–1663

[26]Saxena D, Stotzky G. Bt corn has a higher lignin content than non-Bt corn. Am J Bot, 2001, 88: 1704–1706

[27]Zhang M(张敏), Wang X-C(王校常), Yan W-D(严蔚东), Liang Y-C(梁永超), Shi W-M(施卫明). K+ and Na+ uptake and transport and SOD activity in Bt transgenic cotton seedlings under salt stress. Acta Pedol Sin (土壤学报), 2005, 42(3): 460–467 (in Chinese with English abstract)

[28]Bates S L, Zhao J Z, Roush R T, Shelton A M. Insect resistance management in GM crops: past, present and future. Nat Biotechnol, 2005, 23: 57–62

[29]Ye R J, Huang H Q, Yang Z, Chen T Y, Liu L, Li X H, Chen H, Lin Y J. Development of insect-resistant transgenic rice with Cry1C*-free endosperm. Pest Manag Sci, 2009, 65: 1015–1020

[30]Peng J Y, Kononowicz H, Hodges T K. Transgenic indica rice plants. Theor Appl Genet, 1992, 83: 855–863
[1] Zhang Ying-Xing, Bheel Chander Kumar, Song Yu-Zhen, Wang Yue, Cao Yue, Khound Rituraj, Santra Dipak Kumar, Cao Xiao-Ning, Wang Rui-Yun. Screening and phenotypic characterization of EMS-induced mutants with elite agronomic traits in broomcorn millet [J]. Acta Agronomica Sinica, 2026, 52(5): 1388-1400.
[2] Li Rui, Yu Yi-Wen, Wang Dun-Liang, Tian Ting, Sun Ling-Xiang, Tao Yue-Yue, Sun Hua. Comparative study on the characteristics of rapeseed yield under the rapeseed- oil dual-purpose mode in the middle and lower reaches of the Yangtze River [J]. Acta Agronomica Sinica, 2026, 52(2): 620-630.
[3] Chi Xiao-Yuan, Liu Qing, Zhang Jun, Zhao Xu-Hong, Li Mei, Yu Tian-Yi, Pan Li-Juan, Xu Jing, Jiang Xiao, Yin Xiang-Zhen, Ma Jun-Qing, Chen Na. Field evaluation of salt-alkaline tolerance and trait correlation analysis in different peanut varieties (lines) [J]. Acta Agronomica Sinica, 2026, 52(1): 85-98.
[4] Yang Rui, Chen Jing-Dong, Huang Ying, Zhang Xue-Kun, Zhou Deng-Wen, Liu Qing-Yun, Xu Jing-Song, Xie Ling-Li, Xu Ben-Bo. Region-specific yield optimization strategies for rapeseed (Brassica napus L.) in the middle Yangtze Basin across the 30°N latitude [J]. Acta Agronomica Sinica, 2026, 52(1): 99-117.
[5] QIN Jin-Hua, HONG Wei-Yuan, FENG Xiang-Qian, LI Zi-Qiu, ZHOU Zi-Yu, WANG Ai-Dong, LI Rui-Jie, WANG Dan-Ying, ZHANG Yun-Bo, CHEN Song. Analysis of agronomic and physiological indicators of rice yield and grain quality under nitrogen fertilization management [J]. Acta Agronomica Sinica, 2025, 51(2): 485-502.
[6] CHEN Yu-Ting, DING Xiao-Yu, XU Ben-Bo, ZHANG Xue-Kun, XU Jin-Song, YIN Yan. Effects of climate warming on yield, quality-related and agronomic traits of winter rapeseed (Brassica napus L.) [J]. Acta Agronomica Sinica, 2025, 51(2): 516-525.
[7] LI Yan, LI Yu-Chen, YU Ai-Ping, CHEN Ai-Ping. Comprehensive evaluation of forage yield and nutritional quality in eight Bromus inermis germplasm resources [J]. Acta Agronomica Sinica, 2025, 51(12): 3377-3386.
[8] JIANG Ang-Chen, LI Yan, LI Yu-Chen, ZHANG Jing, CHEN Ai-Ping. Comprehensive evaluation of agronomic traits and seed yield of 21 Bromus inermis germplasm and screening of superior germplasm [J]. Acta Agronomica Sinica, 2025, 51(11): 2958-2970.
[9] GUI Ling-Xing, LING Xi-Tie, TANG Zhao-Cheng, LUO Wen-Zhen, ZHU Pan-Zhen, QIU Ze-Yu, ZHANG Bao-Long. Identification and agronomic characterization of imazamox-resistant dwarf mutants in Ningmai 36 [J]. Acta Agronomica Sinica, 2025, 51(11): 2923-2932.
[10] JIANG Xiao, ZHAO Jian-Xin, BI Jing-Nan, XU Jing, YIN Xiang-Zhen, ZHAO Xu-Hong, PAN Li-Juan, CHEN Na, Ma Jun-Qing, HAN Peng, YANG Zhen, CHI Xiao-Yuan. Effects of major geographic and climatic factors on agronomic traits and quality of peanut in the main production areas of northern China [J]. Acta Agronomica Sinica, 2025, 51(10): 2805-2820.
[11] NIE Bo-Tao, LIU De-Quan, CHEN Jian, CUI Zheng-Guo, HOU Yun-Long, CHEN Liang, QIU Hong-Mei, WANG Yue-Qiang. Analysis and comprehensive evaluation of agronomic and quality traits of spring soybean varieties in northern China [J]. Acta Agronomica Sinica, 2024, 50(9): 2248-2266.
[12] LIU Yuan-Yuan, DONG Jian-Ke, YING Jing-Wen, MEI Wen-Xiang, CHENG Gang, GUO Jing-Jing, JIAO Wen-Biao, SONG Bo-Tao. Creating cold resistant germplasm of potato using Solanum boliviense [J]. Acta Agronomica Sinica, 2024, 50(6): 1384-1393.
[13] LIANG Xing-Wei, YANG Wen-Ting, JIN Yu, HU Li, FU Xiao-Xiang, CHEN Xian-Min, ZHOU Shun-Li, SHEN Si, LIANG Xiao-Gui. Is cob color variation in maize accidental or incidental to any agronomic traits? —An example of nationally approved common hybrids over the years [J]. Acta Agronomica Sinica, 2024, 50(3): 771-778.
[14] XUE Ya-Peng, XIN Xu-Xia, WANG Ruo-Nan, YU Xiao-Han, LIU Shao-Xiong, WANG Rui-Yun, LIU Min-Xuan. Analysis of agronomic, quality traits and genetic diversity of domestic and foreign foxtail millet resources [J]. Acta Agronomica Sinica, 2024, 50(10): 2468-2482.
[15] LIU Jia, GONG Fang-Yi, LIU Ya-Xi, YAN Ze-Hong, ZHONG Xiao-Ying, CHEN Hou-Lin, HUANG Lin, and WU Bi-Hua. Genome-wide association study for agronomic traits in common wheat lines derived from wild emmer wheat [J]. Acta Agronomica Sinica, 2023, 49(5): 1184-1196.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd