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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 820-828.doi: 10.3724/SP.J.1006.2012.00820

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of QTLs for Glycinin (11S) and β-Conglycinin (7S) Fractions of Seed Storage Protein in Soybean by Association Mapping

JIAN Shuang1,2,WEN Zi-Xiang1,LI Hai-Chao1,YUAN Dao-Hua1,LI Jin-Ying1,ZHANG Hui1,YE Yong-Zhong2,LU Wei-Guo1,*   

  1. 1 Institute of Industrial Crops, Henan Academy of Agricultural Sciences / National Subcenter for Soybean Improvement in Zhengzhou / Key Laboratory of Oil Crops in Huanghuai Valleys, Ministry of Agricultural, Zhengzhou 450002, China; 2 College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2011-08-22 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 卢为国, E-mail: 123bean@163.com, Tel: 0371-65733647

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Abstract: The glycinin (11S) and β-conglycinin (7S) are major components of seed storage protein in soybean, which play important roles in the functionality of seed protein. In the present study, association mapping was used to map the QTLs (quantitative trait loci) for glycinin (11S) and β-conglycinin (7S) fractions. One hundred and sixty-six accessions from Chinese soybean minicore collection were genotyped with 134 selected simple-sequence repeat (SSR) markers. The storage protein of each accession was separated by SDS-PAGE method, and gels were scanned for individual subunits of 11S and 7S by ImageQuant TL software. The association analysis between SSR loci and protein subunit components was performed with TASSEL GLM (general linear model) and MLM (mixed linear model) programs. The results showed that, both in 2008 and 2010, fourteen SSR loci associated with the protein subunit components were screened out by GLM program, and five SSR (Satt234, Satt595, Sat_062, Satt583, and Satt291) loci by MLM program, respectively. The high variation of 7S subunits was the main reason that caused 11S/7S ratio variance. Fewer loci were detected to be associated with the protein subunits whose phenotypic variations were higher, which might be due to the more recombination incidents during the evolution of the related genes. Therefore the LD decay distances of these genes were short, some QTLs could not be detected with limited SSR markers. The results of this study are meaningful for the marker assisted selection breeding of soybean varieties with higher protein quality.

Key words: Cultivated soybean [Glycine max (L.) Merr.], Glycinin (11S), &, beta, -conglycinin (7S), Simple-sequence repeat (SSR), Association mapping

[1]Fukushima D. Recent progress of soybean protein foods: chemistry, technology and nutrition. Food Rev Intl, 1991, 7: 323–351

[2]Tayama T E, Kitamura K. Genetic improvement of seed storage protein using three variant allele sofglob-ulinubunts in soybean. Japan J Breed, 1989, 39: 137–147

[3]Huang L-H(黄丽华), Ma H(麻浩), Wang X-S(王显生), Cui G-X(崔国贤). Study on 11S and 7S fractions of seed storage proteins in soybean seeds. Chin J Oil Crop Sci (中国油料作物学报), 2003, 25(3): 20–23 (in Chinese with English abstract)

[4]Liu S-S(刘珊珊), Tian F-D(田福东), Gao L-H(高丽辉), Wang Z-K(王志坤), Ge Y-J(葛玉君), Diao G-Z(刁桂珠), Li W-B(李文滨). Effect of subunit composition of 7S globulin on soybean quality characteristics. Acta Agron Sin (作物学报), 2008, 34(5): 909–913 (in Chinese with English abstract)

[5]Cheng C-L(程翠林), Wang Z-Y(王振宇), Shi Y-G(石彦国), Zhao H-T(赵海田). Effect of soy protein subunits composition and ratio of 7S to11S on the quality and productivity of tofu. China Oils Fats (中国油脂), 2006, 31(4): 16–19 (in Chinese with English abstract)

[6]Liu S-S(刘珊珊), Wang Z-K(王志坤), Ge Y-J(葛玉君), Diao G-Z(刁桂珠), Tian F-D(田福东), Gao L-H(高丽辉), Li W-B(李文滨). Correlations between relative content of individual subunit of 7S globulin and quality characteristics in soybean germplasm. Chin J Oil Crop Sci (中国油料作物学报), 2008, 30(3): 284–289 (in Chinese with English abstract)

[7]Ma H(麻浩), Wang X-S(王显生), Liu C(刘春), Yu T(俞阗), Hao X-Y(郝小燕), Gao W-R(高文瑞), He X-L(何小铃). The content variation of 7S, 11S globulins and their subunits of seed storage protein of 706 Chinese soybean germplasm. Soybean Sci (大豆科学), 2006, 25(1): 11–17 (in English with Chinese abstract)

[8]Wang L-X(王丽侠), Guo S-T(郭顺堂), Fu C-Z(付翠真), Guan R-X(关荣霞), Zhang Q-S(张其属), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). The analysis of 11S/7S in soybean storage protein. Chin Cereals Oil Assoc (中国粮油学报), 2004, 19(4): 64–66 (in Chinese with English abstract)

[9]Liu S-H(刘顺湖), Zhou R-B(周瑞宝), Yu D-Y(喻德跃), Chen S-Y(陈受宜), Gai J-Y(盖钧镒). QTL mapping of protein related traits in soybean [Glycine max (L.) Merr.]. Acta Agron Sin (作物学报), 2009, 35(12): 2139–2149 (in Chinese with English abstract)

[10]Panthee D R, Kwanyuenb P, Samsa C E, Westa D R, Saxtonc A M, Pantalonea V R. Quantitative trait loci for β-conglycinin (7S) and glycinin (11S) fractions of soybean storage protein. J Am Oil Chem Soc, 2004, 81: 1005–1012

[11]Ruth E M. Gene mapping by linkage and association analysis. Mol Biotechnol, 1999, 3: 113–122

[12]Eizenga G C, Agrama H A, Lee F N, Yan W, Jia Y. Identifying novel resistance genes in newly introduced blast resistant rice germplasm. Crop Sci, 2006, 46: 1870–1878

[13]Flint-Garcia S A, Thuillet A, Yu J, Pressoir G, Romero S M, Mitchell S E, Doebley J F, Kresovich S, Goodman M M, Buckler E S. Maize association population: a high resolution platform for QTL dissection. Plant J, 2005, 44: 1054–1064

[14]Jun T H, Van K, Kim M Y, Lee S H. Association analysis using SSR markers to find QTL for seed protein content in soybean. Euphytica, 2008, 162: 179–191

[15]Qiu L-J(邱丽娟), Li Y-H(李英慧), Guan R-X(关荣霞), Liu Z-X(刘章雄), Wang L-X(王丽侠), Chang R-Z(常汝镇). Establishment, representative testing and research progress of soybean core collection and mini core collection. Acta Agron Sin (作物学报), 2009, 35(4): 571–579 (in Chinese with English abstract)

[16]Gai J-Y(盖钧镒), Wang Y-S(汪越胜). A study on the varietal eco-regions of soybeans in China. Sci Agric Sin (中国农业科学), 2001, 34(2): 139–145 (in Chinese with English abstract)

[17]Wang H-L(王红玲), Chen S-G(陈少光), Xiang S-P(向世鹏), Chen C(陈晨), Hao X-Y(郝小燕), Ma H(麻浩). Effects of climate factors on the relative contents of major storage protein fractions and its subunits in soybean seeds. Chin J Oil Crop Sci (中国油料作物学报), 2007, 29(4): 431–437 (in Chinese with English abstract)

[18]Wang X-S(王显生), Ma H(麻浩), Xiang S-P(向世鹏), Zhang G-Z(张国正), Cui G-X(崔国贤). The resolving effect of soybean storage protein subunits under different separation gel concentrations of SDS-PAGE. Chin J Oil Crop Sci (中国油料作物学报), 2004, 26(2): 75–80 (in Chinese with English abstract)

[19]Doyle J J, Doyle J I. Isolation of plant DNA from fresh tissue. Focus, 1990, 12: 149–151

[20]Song Q J, Marek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregan P B. A new integrated genetic linkage map of the soybean. Theor Appl Genet, 2004, 109: 122–128

[21]Wen Z-X(文自翔), Zhao T-J(赵团结), Zheng Y-Z(郑永战), Liu S-H(刘顺湖), Wang C-E(王春娥), Wang F(王芳), Gai J-Y(盖钧镒). Association analysis of agronomic and quality traits with SSR markers in Glycine max and Glycine soja in China: I. Population structure and associated markers. Acta Agron Sin (作物学报), 2008, 34(7): 1169–1178 (in Chinese with English abstract)

[22]Evanno G, Regnaut S, Goudet J. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol, 2005, 14: 2611–2620

[23]Zhu Y L, Song Q J, Hyten D L, Van Tassell C P, Matukumalli L K, Grimm D R, Hyatt S M, Fickus E W, Young N D, Cregan P B. Single nucleotide polymorphisms in soybean. Genetics, 2003, 163: 1123–1134

[24]Li Y H, Guan R X, Liu Z X, Ma Y S, Qiu L J. Genetic structure and diversity of cultivated soybean (Glycine max (L.) Merr.) landraces in China. Theor Appl Genet, 2008, 117: 857–871

[25]Yu J M, Gael P, William H B. A unified mixed-model method for association mapping that accounts for multiple levels of relatedness. Nat Genet, 2006, 38(2): 203–207

[26]Gupta P K, Rustgi S, Kulwal P L. Linkage disequilibrium and association studies in higher plants: present status and future prospects. Plant Mol Biol, 2005, 57: 461–485

[27]Specht J E, Chase K, Macrander M, Graef G L, Chung J, Markwell J P, Germann M, Orf J H, Lark K G. Soybean response to water: a QTL analysis of drought tolerance. Crop Sci, 2001, 41: 493–509

[28]Chung J, Babka H L, Graef G L, Staswick P E, Lee D J, Cregan P B, Shoemaker R C, Specht J E. The seed protein, oil, and yield QTL on soybean linkage group I. Crop Sci, 2003, 43: 1053–1067

[29]Poysa V, Woodrow L, Yu K. Effect of soy protein subunit composition on tofu quality. Food Res Intl, 2006, 39: 309–317

[30]Harada K, Barker S, Goldberg R. Soybean β-conglycinin genes are clustered in several DNA regions and are regulated by transcriptional and posttranscriptional processes. Plant Cell, 1989, 1: 415–425

[31]Moreira M A, Hermodson M A, Larkins B A, Nielsen N C. Partial characterization of the acidic and bacidic polypeptides of glycinin. Biol Chem, 1979, 254: 9921–9926

[32]Huang L-H(黄丽华), Ma H(麻浩), Wang X-S(王显生), Cui G-X(崔国贤). Study on 11S and 7S fractions of seed storage proteins in soybean seeds. Chin J Oil Crop Sci (中国油料作物学报), 2003, 25(3): 20–23 (in Chinese with English abstract)

[33]Chen H-M(陈海敏), Hua Y-F(华欲飞). Effects of various cultivars on functionalities of soy protein. China Western Cereals Oils Technol (西部粮油科技), 2001, 26(3): 36–38 (in Chinese with English abstract)
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