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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 808-813.doi: 10.3724/SP.J.1006.2012.00808

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Analysis on Full-length cDNA Sequence of Ubiquitin-conjugating Enzyme Gene from Oryza meyeriana Baill.

JIANG Chun-Miao1,2,HUANG Xing-Qi1,FU Jian1,YU Teng-Qiong1,ZHONG Qiao-Fang1,LI Ding-Qin1,YIN Fu-You1,ZHANG Dun-Yu1,WANG Ling-Xian1,CHENG Zai-Quan1,*   

  1. 1 Biotechnology & Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China; 2 College of Life Science, Yunnan University, Kunming 650091, China
  • Received:2011-10-19 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 程在全, E-mail: czquan-99@163.com, Tel: 13888556318, 0871-5140200

Abstract: Based on the bioinformatic analysis of the ESTs from the subtraction cDNA library of Oryza meyeriana, we cloned one of the ESTs which has the homolog function of ubiquitin-conjugating enzyme and got a full-length cDNA sequence of ubiquitin-conjugating enzyme gene by RACE, and named it OmE2 (Oryza meyerianaBaill ubiquitin-conjugating enzyme, OmE2). This full-length cDNA sequence has 917 bp including 528 bp open reading frame encoding a protein of 175 amino acids, with a predicted molecular mass of 19.3 kD and pI of 9.32. OmE2 protein which had significant amino sequence identity and similarity with ubiquitin-conjugating enzyme from other species contained the conserved sequence and cysteine residue in active sites of ubiquitin-conjugating family. OmE2 had three trans-membrane domains. It is predicted that OmE2 protein is a kind of ubiquitin-conjugating protein and transmembrane protein. The expression of OmE2 inOryza meyeriana was induced by Xanthomonas campestris pv. oryzae. Therefore OmE2 gene which was first found in wild rice may participate in the signal transduction and the resistance response in Oryza meyeriana.

Key words: Oryza meyeriana Baill., Ubiquitin-conjugating enzyme, Sequence analysis

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