Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 808-813.doi: 10.3724/SP.J.1006.2012.00808

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Analysis on Full-length cDNA Sequence of Ubiquitin-conjugating Enzyme Gene from Oryza meyeriana Baill.

JIANG Chun-Miao1,2,HUANG Xing-Qi1,FU Jian1,YU Teng-Qiong1,ZHONG Qiao-Fang1,LI Ding-Qin1,YIN Fu-You1,ZHANG Dun-Yu1,WANG Ling-Xian1,CHENG Zai-Quan1,*   

  1. 1 Biotechnology & Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China; 2 College of Life Science, Yunnan University, Kunming 650091, China
  • Received:2011-10-19 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 程在全, E-mail: czquan-99@163.com, Tel: 13888556318, 0871-5140200

PDF

1266

Cited

4

Abstract: Based on the bioinformatic analysis of the ESTs from the subtraction cDNA library of Oryza meyeriana, we cloned one of the ESTs which has the homolog function of ubiquitin-conjugating enzyme and got a full-length cDNA sequence of ubiquitin-conjugating enzyme gene by RACE, and named it OmE2 (Oryza meyerianaBaill ubiquitin-conjugating enzyme, OmE2). This full-length cDNA sequence has 917 bp including 528 bp open reading frame encoding a protein of 175 amino acids, with a predicted molecular mass of 19.3 kD and pI of 9.32. OmE2 protein which had significant amino sequence identity and similarity with ubiquitin-conjugating enzyme from other species contained the conserved sequence and cysteine residue in active sites of ubiquitin-conjugating family. OmE2 had three trans-membrane domains. It is predicted that OmE2 protein is a kind of ubiquitin-conjugating protein and transmembrane protein. The expression of OmE2 inOryza meyeriana was induced by Xanthomonas campestris pv. oryzae. Therefore OmE2 gene which was first found in wild rice may participate in the signal transduction and the resistance response in Oryza meyeriana.

Key words: Oryza meyeriana Baill., Ubiquitin-conjugating enzyme, Sequence analysis

[1]Dong F-C(董发才), Song C-P(宋纯鹏). The ubiquitin and physiological functions of plant cell. Plant Physiol Commun (植物生理学通讯), 1999, 35(1): 54–59 (in Chinese)

[2]Ingvardsen C, Veierskov B. Ubiquitin and proteasome-dependent proteolysis in plant. Physiol Plant, 2001, 112: 451–459

[3]Smalle J, Viertra R D. The ubiquitin 26S proteasome proteolytic pathway. Annu Rev Plant Biol, 2004, 16: 3181–3195

[4]Dreher K, Callis J. Ubiquitin, hormones and biotic stress in plants. Ann Bot, 2007, 99: 787–822

[5]Wang J-L(王金利), Shi S-Q(史胜青), Jia L-Q(贾利强), Jiang Z-P(江泽平). Progress on functions of ubiquitin-conjugating enzyme (E2) in plants. Biotechnol Bull (生物技术通报), 2010, (4): 7–9 (in Chinese with English abstract)

[6]Pickart C M. Mechanisms underlying ubiquitination. Annu Revbiochem, 2001, 70: 503–533

[7]Lau O S, Deng X W. Effect of Arabidopsis COP10 ubiquitin E2 enhancement activity across E2 families and functional conservation among its canonical homologs. Biochem J, 2009, 418: 683–690

[8]Lin W Y, Lin S I, Chiou T I. Molecular regulators of phosphate homeostasis in plants. J Exp Bot, 2009, 60: 1427–1438

[9]Feussner K, Feussner I, Leopold I. Isolation of a cDNA coding for an uniquitin-conjugating enzyme UBC1 of tomato—the first stress-induced UBC of higher plants. FEBS Lett, 1997, 409: 211–215

[10]Xu C-X(徐晨曦), Jiang J(姜静), Liu T-T(刘甜甜), Wang Y-C(王玉成), Liu G-F(刘桂丰), Yang C-P(杨传平). Sequence analysis and function determination of E2s gene from Tamarix androssowii. Northeast For Univ (东北林业大学学报), 2007, 35(11): 1–4 (in Chinese with English abstract)

[11]Lu Y-S(陆月赏), Liu Y-H(刘颖慧), Zhang D-F(张登峰), Shi Y-S(石云素), Song Y-C(宋燕春), Wang T-Y(王天宇), Yang D-G(杨德光), Li Y(黎裕). Identification and expression analysis of ZmERD16: a ubiquitin extension protein gene in maize (Zea mays L.). Acta Agron Sin (作物学报), 2010, 36(7): 1075–1083 (in Chinese with English abstract)

[12]Yi L-F(易乐飞), Liu C-W(刘楚吾), Wang P(王萍), Zhou X-H(周向红). cDNA cloning and characterization of a novel ubiquitin-conjugating enzyme gene from Porphyra yezoensis Ueda. J Fish China (水产学报), 2009, 33(5): 719–725 (in Chinese with English abstract)

[13]Guo Q-F(郭启芳). Cloning and Transformation of Ubiquitin Gene in Winter Wheat and Its Expression. MS Dissertation of Shandong Agricultural University, 2004 (in Chinese with English abstract)

[14]Yan H-J(宴慧君). Construction and Analysis of Oryza meyeriana Suppression Subtractive Hybridization Library under Xanthomonas campestris pv. oryza Stress. MS Dissertation of Yunnan University, 2007 (in Chinese with English abstract)

[15]Xu L, Menard R, Berr A. The E2 ubiquitin-conjugation enzymes, AtUBC1 and AtUBC2, play redundant role and are involved in activation of FLC expression and repression of flowering in Arabidopsis thaliana. Plant J, 2009, 57: 279–288

[16]Song J-H(宋江华), Zhang L-X(张立新). Progress on the transmembrane protein in plants. J Biol (生物学杂志), 2009, 26(6): 62–64 (in Chinese with English abstract)

[17]Chen X F, Wang B Y, Wu R. A gibberellin-stimulated ubiquitin-conjugating enzyme gene is involved in ?-amylase gene expression in rice aleurone. Plant Mol Biol, 1995, 29: 787–795
[1] XU Yu-Chao,HOU Xi-Lin,XU Wei-Wei,SHEN Lu-Lu,ZHANG Shi-Lin,LIU Shi-Tuo,HU Chun-Mei. Cloning and Expression Analysis of Anthocyanidin Synthase Gene BrcANS from Purple Non-heading Chinese Cabbage [J]. Acta Agron Sin, 2016, 42(06): 850-859.
[2] WANG Xiao-Hong, ZHU Pan-Pan, LIANG Yan-Mei, HAN Shu-Mei, ZHAO Ai-Chun, WANG Chuan-Hong, LU Cheng, YU Mao-De. Molecular Cloning and Functional Analysis of Polygalacturonase-Inhibiting Protein Gene MaPGIP1 from Mulberry (Morus atropurpurea Roxb.) [J]. Acta Agron Sin, 2015, 41(09): 1361-1371.
[3] LI Wei,SHANG Hai-Hong,WANG Shao-Gan,FAN Sen-Miao,LI Jun-Wen,LIU Ai-Ying,SHI Yu-Zhen,GONG Ju-Wu,GONG Wan-Kui,WANG Tao,BAI Zhi-Chuan,YUAN You-Lu. Cloning and Expression Analysis of Three Aquaporin Genes in Upland Cotton (Gossypium hirsutum L.) [J]. Acta Agron Sin, 2013, 39(02): 222-229.
[4] LI Shuan-Zhu,WAN Yong-Shan,LIU Feng-Zhen. Cloning and Bioinformatic Analysis of γ-Tocopherol Methyltransferase Gene (γ-TMT) in Peanut [J]. Acta Agron Sin, 2012, 38(10): 1856-1863.
[5] GENG Wei-Dong,LI Yan-Jun,ZHANG Xin-Yu,ZHU Hua-Guo,SUN Jie. Molecular Cloning and Expression Analysis of GhSAMDC at Low Temperature Stress in Cotton (Gossypium hirsutum L.) [J]. Acta Agron Sin, 2012, 38(09): 1649-1656.
[6] DONG Jin-Yu,WAN Yong-Shan,LIU Feng-Zhen. Sequence Analysis of Δ9-Stearoyl-ACP Desaturase Gene (SAD) in Peanut [J]. Acta Agron Sin, 2012, 38(07): 1167-1177.
[7] LIU Yu-Hui,WANG Li,YANG Hong-Yu,YU Bin,LI Yuan-Ming,ZHANG Jun-Lian,WANG Di. Cloning of Granule-Bound Starch Synthase Gene and Construction of Its RNAi Vector in Potato Tuber [J]. Acta Agron Sin, 2012, 38(07): 1187-1195.
[8] YANG Kun,ZHOU Yong-Xiang,ZHANG He-Cui,ZHAO Yong-Bin,YANG Yong-Jun,LU Jun-Xing,ZHU Li-Quan,XUE Li-Yan,Lü Jun,GAO Qi-Guo. Cloning and Expression Characteristics of EXO70A1 from Brassica oleracea, Brassica campestris and Brassica napus [J]. Acta Agron Sin, 2012, 38(04): 578-588.
[9] WANG Wang-Tian, ZHANG Jin-Wen, WANG Di, ZHANG Jun-Lian, SI Fu-Jun, TAO Shi-Hang. Cloning of Rhamnosyl Transferase Gene and Construction of Its RNAi Vector in Potato [J]. Acta Agron Sin, 2011, 37(11): 1926-1934.
[10] ZHANG Qiang-Lin, ZHAO Yan, LI Xiao-Wei, ZAI Ying, ZHANG Yan, WANG Yang, LI Jing-Wen, WANG Qiang-Yu. Cloning and Activity Analysis of Soybean SACPD-C Promote [J]. Acta Agron Sin, 2011, 37(07): 1205-1211.
[11] WANG Ming-Xia,GAO Xiang,CHEN Qi-Jiao,DONG Jian,ZHAO Wan-Chun,LI Yan-Liang et al. Gene Cloning and Sequence Analysis of γ-Gliadin Genes from Wheat Cultivar Shann 253 [J]. Acta Agron Sin, 2010, 36(3): 526-532.
[12] XU Ai-Xia,HUANG Zhen, MA Chao-Zhi, XIAO En-Shi,ZHANG Xiu-Sen,TU Jin-Xing,FU Ting. FAE1 Sequence Characteristics and Its Relationship with Erucic Acid Content in Brassica juncea [J]. Acta Agron Sin, 2010, 36(05): 794-800.
[13] DING Yong;CHEN Qing-Bo;XU Chun-Lei;CHANG Wei;GAN Li. Cloning and Expression of BnClo1 Gene from Brassica napus [J]. Acta Agron Sin, 2008, 34(11): 1921-1928.
[14] GAO Yuan;CHEN Xin-Bo;LONG Song-Hua;DENG Xin;ZOU Jie;LIU Ai-Ling. Cloning and Sequence Analysis of Partial Fragments of Critical Genes Involved in Flax Lignin Synthesis [J]. Acta Agron Sin, 2008, 34(02): 337-340.
[15] WANG Jin-Ming;DING Zai-Song;ZHANG Gui-Fang;ZHAO Ming. Cloning and Sequence Analysis of pdk Gene in [Echinochloa crusgalli var. frumentacea (Roxb.) W. F. Wight] [J]. Acta Agron Sin, 2007, 33(06): 927-930.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd