Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1167-1177.doi: 10.3724/SP.J.1006.2012.01167

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Sequence Analysis of Δ9-Stearoyl-ACP Desaturase Gene (SAD) in Peanut

DONG Jin-Yu,WAN Yong-Shan*,LIU Feng-Zhen*   

  1. State Key Laboratory of Crop Biology / Shandong Key Laboratory of Crop Biology / Agronomy College of Shandong Agricultural University, Tai’an 271018, China
  • Received:2011-12-29 Revised:2012-04-15 Online:2012-07-12 Published:2012-05-11
  • Contact: 万勇善, E-mail: yswan@sdau.edu.cn, Tel: 0538-8241540; 刘风珍, E-mail: liufz@sdau.edu.cn, Tel: 0538-8241540 E-mail:dongjinyu86@163.com

PDF

1504

Abstract: The peanut (Arachis hypogaea L.) cultivars are an allotetraploid consisting of A and B genomes. Δ9-Stearoyl-ACP desaturase (SAD) is a key enzyme that catalyzes the conversion of stearoyl-ACP to oleoyl-ACP, and finally controls the content of oleic acid as well as the proportion of saturated to unsaturated fatty acids. By using the primers based on the peanut cDNA sequence of SAD (AF172728), genomic DNAs were amplified from the wild diploid species A. duranensis and A. ipaensis, and from the cultivated accessions of peanut Fenghua 2, Shanhua 7 and Puyangdatuoyang, respectively. Two isoforms of the genomic SAD were identified and named as FhgSAD-1 and FhgSAD-2 from the cultivated peanut accessions. In addition, two isoforms of SAD cDNA were isolated and named as FhrSAD-1 and FhrSAD-2 from Fenghua 2. Comparison of the genomic sequences with cDNAs revealed that there were two introns in the SAD genomic sequences. Sequences alignment showed that the similarity between FhgSAD-1 and FhgSAD-2 in nucleotide level was over 97.5%, with 69 different sites in total, including 62 SNP sites and six variation sites of endonuclease recognition. The cDNA sequence similarity between FhrSAD-1 and FhrSAD-2 was 98.6%, with 98.9% nucleotides identity in coding region. Deduced amino acid sequences revealed that only one difference occurred in serine gathering area of 17PSSSSSSSSSSFSL30. gSAD-1 shared 99.9% nucleotide sequence homology with gSAD-A, while gSAD-2 was the same as gSAD-B. According to the phylogenetic tree, it is assumed that gSAD-1 and gSAD-2 may come from the A and B genome, respectively. The results revealed the characteristics of SAD sequences from different genomes of peanut, and provided important basis for exploring gene expression regulation and fatty acid component improvement in peanut seeds.

Key words: Peanut, Stearoyl-ACP desaturase (SAD), Sequence analysis

[1]Wan S-B(万书波). Peanut Quality (花生品质学). Beijing: China Agricultural Science and Technology Press, 2005. pp 2–10 (in Chinese)

[2]Chu Y, Holbrook C C, Ozias-Akins P. Two alleles of ahFAD2B control the the high oleic acid trait in cultivated peanut. Crop Sci, 2009, 49: 2029–2036

[3]Yukawa Y, Takaiwa F, Shojik K, Masuda K, Yamada K. Structure and expression of two seed-specific cDNA encoding stearoyl-acyl carrier protein desaturase from sesame, Sesamum indicum. Plant Cell Physiol, 1996, 37: 201–205

[4]Li X-D(李晓丹), Cao Y-L(曹应龙), Hu Y(胡亚), Xiao L(肖玲), Wu Y-H(武玉花), Wu G(吴刚), Lu C-M(卢长明). Fatty acid accumulation pattern in developing seeds of peanut. Chin J Oil Crop Sci (中国油料作物学报), 2009, 31(2): 157–162 (in Chinese with English abstract)

[5]Wendy C, Paolo L, Nunzia S, Monica D P, Paola S, Virginia C, Noreen M C, Alan M M, Peter M, Tony A K, Philip J D, Stefania G, Teodoro C. Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance. Transgenic Res, 2008, 17: 769–782

[6]Kachroo A, Shanklin J, Whittle E, Lapchyk L, Hildebrand D, Kachroo P. The Arabidopsis stearoyl-acyl carrier protein-desaturase family and the contribution of leaf isoforms to oleic acid synthesis. Plant Mol Biol, 2007, 63: 257–271

[7]Byfield G E, Xue H, Upchurch R G. Two genes from soybean encoding soluble Δ9-stearoyl-ACP desaturase. Crop Sci, 2006, 46: 840–846

[8]Ping Z, Joseph W B, Robert G U, Edward W, John S, Ralph E D. Mutations in a Δ9-Stearoyl-ACP-desaturase gene are associated with enhanced stearic acid levels in soybean seeds. Crop Sci, 2008, 48: 2305–2313

[9]Aardra K, John S, Edward W, Ludmila L, David H, Pradeep K. The Arabidopsis stearoyl-acyl carrier protein-desaturase family and the contribution of leaf isoforms to oleic acid synthesis. Plant Mol Biol, 2007, 63: 257–271

[10]Luo T, Deng W Y, Zeng J, Zhang F I. Cloning and characterization of a stearoyl-Acyl carrier protein desaturase gene from Cinnamomum longepaniculatum. Plant Mol Biol Rep, 2009, 27: 13–19

[11]Florin S, Yael B, Arnon B, Ilan H, Ran H. Identification and molecular characterization of homeologous Δ9-Stearoyl-acyl carrier protein desaturase3 genes from the allotetraploid peanut (Arachis hypogaea). Plant Mol Biol Rep, 2010, 29: 232–241

[12]Whittle E, Cahoon E B, Subrahmanyam S, Shanklin J. A multifunctional acyl-acyl carrier protein desaturase from Hedera helix L. (English ivy) can synthesize 16- and 18-carbon monoene and diene products. J Biol Chem, 2005, 280: 28169–28176

[13]Zaborowska Z, Starzycki M, Femiak I, Swiderski M, Legocki A B. Yellow lupine gene encoding stearoyl-ACP desaturase-organization, expression and potential application. Acta Biochimicn Polonicn, 2002, 49: 29–42

[14]Chen M N, Ren Z K, Chi X Y, Pan L J, Yu S L, Yang Q L. Isolation, characterization and expression analysis of stearoyl-ACP desaturase gene from Kosteletzkya virginica. Bioinform Biomed Engin (iCBBE), 2010, 4: 1–5

[15]Shah F H, Rashid O, San C T. Temporal regulation of two isoforms of cDNA clones encoding delta 9-stearoyl-ACP desaturase from oil palm (Elaies guineensis). Plant Sci, 2000, 152: 27–33

[16]Liu Q, Singh S P, Green A G. High-steric and oleic cottonseed oils produced by hairpin RNA-mediated post-transcriptional gene silencing. Plant Physiol, 2002, 129: 1–12

[17]Wendy C, Paolo L, Nunzia S, Monica D P, Paola S, Virginia C, Noreen M C, Alan M M, Peter M, Tony A K, Philip J D, Stefania G, Teodoro C. Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance. Transgenic Res, 2008, 17: 769–782

[18]Thompson G A, Scherer D E, Aken S F, Kenny J W, Young H L, Shintani D K, Kridl J C, Knauf V C. Primary structures of the precursor and mature forms of stearoyl-acyl carrier protein desaturase from safflower embryos and requirement of ferredoxin for enzyme activity. Proc Natl Acad Sci USA, 1991, 88: 2578–2582

[19]Zhang D-Q(张党权), Tan X-F(谭晓风), Chen H-P(陈鸿鹏), Zeng Y-L(曾艳玲), Jiang Y(蒋瑶), Li W(李魏), Hu F-M(胡芳名). Full-length cDNA cloning and bioinformatic analysis of Camellia oleifera SAD. Sci Silv Sin (林业科学), 2008, 44(2): 155–159 (in Chinese with English abstract)

[20]Dong S-Z(董胜张), Ye G-Y(叶恭银), Liu C-L(刘朝良). Research progress in molecular evolution of yolk proteins in insects. Acta Entomol Sin (昆虫学报), 2008, 51(11): 1 196–1 209 (in Chinese with English abstract)

[21]Sappington T W, Raikhel A S. Molecular characteristics of insect vitellogenin and vitellogenin receptors. Insect Biochem Mol Biol, 1998, 28: 277–300

[22]Lindqvist Y, Huang W, Schneider G, Shanklin J. Crystal structure of delta9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to other diiron proteins. EMBO J, 1996, 15: 4081–4092

[23]Krapovickas A, Gregory W C. Taxonomia del género Arachis (Leguminosae). Bonplandia, 1994, 8: 1–186

[24]Valls J F M, Simpson C E: New species of Arachis from Brazil, Paraguay, and Bolivia. Bonplandia, 2005, 14: 35–64

[25]Smartt J, Gregory W C, Gregory M P. The genomes of Arachis hypogaea L. cytogenetic studies of putative genome donor. Euphytica, 1978, 27: 665–675

[26]Moretzsohn M C, Hopkins M S, Mitchell S E, Kresovich S, Valls J F M, Ferreira M E. Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome. BMC Plant Biol, 2004, 4: 11

[27]Seijo G, Lavia G I, Fernández A, Krapovickas A, Ducasse D A, Bertioli D J, Moscone E A. Genomic relationships between the cultivated peanut (Arachis hypogaea Leguminosae) and its close relatives revealed by double GISH. Am J Bot, 2007, 94: 1963–1971

[28]Milla S R, Isleib T G, Stalker H T. Taxonomic relationships among Arachis sect. Arachis species as revealed by AFLP markers. Genome, 2005, 48: 1–11

[29]Fávero A P, Simpson C E, Valls J F M, Vello N A. Study of the evolution of cultivated peanut through crossability studies among Arachis ipaënsis, A. duranensis, and A. hypogaea. Crop Sci, 2006, 46: 1546–1552

[30]Jung S, Tate P L, Horn R, Kochert G, Moore K, Abbott A G. The phylogenetic relationship of possible progenitors of the cultivated peanut. J Hered, 2003, 94: 334–340
[1] YANG Huan, ZHOU Ying, CHEN Ping, DU Qing, ZHENG Ben-Chuan, PU Tian, WEN Jing, YANG Wen-Yu, YONG Tai-Wen. Effects of nutrient uptake and utilization on yield of maize-legume strip intercropping system [J]. Acta Agronomica Sinica, 2022, 48(6): 1476-1487.
[2] LI Hai-Fen, WEI Hao, WEN Shi-Jie, LU Qing, LIU Hao, LI Shao-Xiong, HONG Yan-Bin, CHEN Xiao-Ping, LIANG Xuan-Qiang. Cloning and expression analysis of voltage dependent anion channel (AhVDAC) gene in the geotropism response of the peanut gynophores [J]. Acta Agronomica Sinica, 2022, 48(6): 1558-1565.
[3] DING Hong, XU Yang, ZHANG Guan-Chu, QIN Fei-Fei, DAI Liang-Xiang, ZHANG Zhi-Meng. Effects of drought at different growth stages and nitrogen application on nitrogen absorption and utilization in peanut [J]. Acta Agronomica Sinica, 2022, 48(3): 695-703.
[4] HUANG Li, CHEN Yu-Ning, LUO Huai-Yong, ZHOU Xiao-Jing, LIU Nian, CHEN Wei-Gang, LEI Yong, LIAO Bo-Shou, JIANG Hui-Fang. Advances of QTL mapping for seed size related traits in peanut [J]. Acta Agronomica Sinica, 2022, 48(2): 280-291.
[5] WANG Ying, GAO Fang, LIU Zhao-Xin, ZHAO Ji-Hao, LAI Hua-Jiang, PAN Xiao-Yi, BI Chen, LI Xiang-Dong, YANG Dong-Qing. Identification of gene co-expression modules of peanut main stem growth by WGCNA [J]. Acta Agronomica Sinica, 2021, 47(9): 1639-1653.
[6] WANG Jian-Guo, ZHANG Jia-Lei, GUO Feng, TANG Zhao-Hui, YANG Sha, PENG Zhen-Ying, MENG Jing-Jing, CUI Li, LI Xin-Guo, WAN Shu-Bo. Effects of interaction between calcium and nitrogen fertilizers on dry matter, nitrogen accumulation and distribution, and yield in peanut [J]. Acta Agronomica Sinica, 2021, 47(9): 1666-1679.
[7] SHI Lei, MIAO Li-Juan, HUANG Bing-Yan, GAO Wei, ZHANG Zong-Xin, QI Fei-Yan, LIU Juan, DONG Wen-Zhao, ZHANG Xin-You. Characterization of the promoter and 5'-UTR intron in AhFAD2-1 genes from peanut and their responses to cold stress [J]. Acta Agronomica Sinica, 2021, 47(9): 1703-1711.
[8] GAO Fang, LIU Zhao-Xin, ZHAO Ji-Hao, WANG Ying, PAN Xiao-Yi, LAI Hua-Jiang, LI Xiang-Dong, YANG Dong-Qing. Source-sink characteristics and classification of peanut major cultivars in North China [J]. Acta Agronomica Sinica, 2021, 47(9): 1712-1723.
[9] ZHANG He, JIANG Chun-Ji, YIN Dong-Mei, DONG Jia-Le, REN Jing-Yao, ZHAO Xin-Hua, ZHONG Chao, WANG Xiao-Guang, YU Hai-Qiu. Establishment of comprehensive evaluation system for cold tolerance and screening of cold-tolerance germplasm in peanut [J]. Acta Agronomica Sinica, 2021, 47(9): 1753-1767.
[10] XUE Xiao-Meng, WU JIE, WANG Xin, BAI Dong-Mei, HU Mei-Ling, YAN Li-Ying, CHEN Yu-Ning, KANG Yan-Ping, WANG Zhi-Hui, HUAI Dong-Xin, LEI Yong, LIAO Bo-Shou. Effects of cold stress on germination in peanut cultivars with normal and high content of oleic acid [J]. Acta Agronomica Sinica, 2021, 47(9): 1768-1778.
[11] HAO Xi, CUI Ya-Nan, ZHANG Jun, LIU Juan, ZANG Xiu-Wang, GAO Wei, LIU Bing, DONG Wen-Zhao, TANG Feng-Shou. Effects of hydrogen peroxide soaking on germination and physiological metabolism of seeds in peanut [J]. Acta Agronomica Sinica, 2021, 47(9): 1834-1840.
[12] ZHANG Wang, XIAN Jun-Lin, SUN Chao, WANG Chun-Ming, SHI Li, YU Wei-Chang. Preliminary study of genome editing of peanut FAD2 genes by CRISPR/Cas9 [J]. Acta Agronomica Sinica, 2021, 47(8): 1481-1490.
[13] DAI Liang-Xiang, XU Yang, ZHANG Guan-Chu, SHI Xiao-Long, QIN Fei-Fei, DING Hong, ZHANG Zhi-Meng. Response of rhizosphere bacterial community diversity to salt stress in peanut [J]. Acta Agronomica Sinica, 2021, 47(8): 1581-1592.
[14] HUANG Bing-Yan, SUN Zi-Qi, LIU Hua, FANG Yuan-Jin, SHI Lei, MIAO Li-Juan, ZHANG Mao-Ning, ZHANG Zhong-Xin, XU Jing, ZHANG Meng-Yuan, DONG Wen-Zhao, ZHANG Xin-You. Genetic analysis of fat content based on nested populations in peanut (Arachis hypogaea L.) [J]. Acta Agronomica Sinica, 2021, 47(6): 1100-1108.
[15] XU Jing, PAN Li-Juan, LI Hao-Yuan, WANG Tong, CHEN Na, CHEN Ming-Na, WANG Mian, YU Shan-Lin, HOU Yan-Hua, CHI Xiao-Yuan. Expression pattern analysis of genes related to lipid synthesis in peanut [J]. Acta Agronomica Sinica, 2021, 47(6): 1124-1137.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd