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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1167-1177.doi: 10.3724/SP.J.1006.2012.01167

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Sequence Analysis of Δ9-Stearoyl-ACP Desaturase Gene (SAD) in Peanut

DONG Jin-Yu,WAN Yong-Shan*,LIU Feng-Zhen*   

  1. State Key Laboratory of Crop Biology / Shandong Key Laboratory of Crop Biology / Agronomy College of Shandong Agricultural University, Tai’an 271018, China
  • Received:2011-12-29 Revised:2012-04-15 Online:2012-07-12 Published:2012-05-11
  • Contact: 万勇善, E-mail: yswan@sdau.edu.cn, Tel: 0538-8241540; 刘风珍, E-mail: liufz@sdau.edu.cn, Tel: 0538-8241540 E-mail:dongjinyu86@163.com

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Abstract: The peanut (Arachis hypogaea L.) cultivars are an allotetraploid consisting of A and B genomes. Δ9-Stearoyl-ACP desaturase (SAD) is a key enzyme that catalyzes the conversion of stearoyl-ACP to oleoyl-ACP, and finally controls the content of oleic acid as well as the proportion of saturated to unsaturated fatty acids. By using the primers based on the peanut cDNA sequence of SAD (AF172728), genomic DNAs were amplified from the wild diploid species A. duranensis and A. ipaensis, and from the cultivated accessions of peanut Fenghua 2, Shanhua 7 and Puyangdatuoyang, respectively. Two isoforms of the genomic SAD were identified and named as FhgSAD-1 and FhgSAD-2 from the cultivated peanut accessions. In addition, two isoforms of SAD cDNA were isolated and named as FhrSAD-1 and FhrSAD-2 from Fenghua 2. Comparison of the genomic sequences with cDNAs revealed that there were two introns in the SAD genomic sequences. Sequences alignment showed that the similarity between FhgSAD-1 and FhgSAD-2 in nucleotide level was over 97.5%, with 69 different sites in total, including 62 SNP sites and six variation sites of endonuclease recognition. The cDNA sequence similarity between FhrSAD-1 and FhrSAD-2 was 98.6%, with 98.9% nucleotides identity in coding region. Deduced amino acid sequences revealed that only one difference occurred in serine gathering area of 17PSSSSSSSSSSFSL30. gSAD-1 shared 99.9% nucleotide sequence homology with gSAD-A, while gSAD-2 was the same as gSAD-B. According to the phylogenetic tree, it is assumed that gSAD-1 and gSAD-2 may come from the A and B genome, respectively. The results revealed the characteristics of SAD sequences from different genomes of peanut, and provided important basis for exploring gene expression regulation and fatty acid component improvement in peanut seeds.

Key words: Peanut, Stearoyl-ACP desaturase (SAD), Sequence analysis

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