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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1178-1186.doi: 10.3724/SP.J.1006.2012.01178

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Rice Pyramiding Breeding using Sheath Blight Resistance QTL qSB-9TQ and Stripe Disease Resistance Gene Stv-bi

CHEN Zong-Xiang1,ZUO Shi-Min1,*,ZHANG Ya-Fang1,ZHU Jun-Kai1,WANG Long-Ping1,FENG Fan1,MA Yu-Yin2,PAN Xue-Biao1,*   

  1. 1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genetics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; 2 Yangzhou Polytechnic College, Yangzhou 225000, China
  • Received:2012-02-14 Revised:2012-04-20 Online:2012-07-12 Published:2012-05-11
  • Contact: 左示敏, 潘学彪, E-mail: shuidao@yzu.edu.cn, Tel: 0514-87972136 E-mail:czx@yzu.edu.cn

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Abstract: Indica rice cultivar Teqing and japonica rice cultivar Zhendao 88 were used as the donor parents with the sheath blight (SB) resistance QTL qSB-9TQ and the resistance gene Stv-bi, respectively. Commercial rice japonica cultivars, Wuyujing 3 and Wujing 15, were used as the receipt parents to cross and backcross with the donor parents, respectively. Flanked polymorphic markers to the two loci were developed and employed to select the target loci via marker assisted selection (MAS) in each backcross and self-cross progeny. Till backcross generation BC4F1, pyramiding was performed by crossing the selected individuals with different integression genes. At F3 generation, the pyramiding lines with homozygosity at both gene loci were obtained. Results of disease resistance evaluation indicated that both pyramiding lines were resistant to stripe disease, and increased resistance to SB with a reducing SB sating of 1.1–1.6 in Wuyujing 3 background and 0.8–1.4 in Wujing 15 background based on a “0–9” SB rating scale. Combining the results of resistance phenotype at the low backcrossing generation, we thought the markers developed in this study were efficient in selecting the two genes by MAS. Some questions associated with SB resistance breeding and pyramiding breeding via MAS were discussed.

Key words: Marker assisted selection, Sheath blight, Stripe disease, Pyramiding breeding

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