Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1167-1177.doi: 10.3724/SP.J.1006.2012.01167

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Sequence Analysis of Δ9-Stearoyl-ACP Desaturase Gene (SAD) in Peanut

DONG Jin-Yu,WAN Yong-Shan*,LIU Feng-Zhen*   

  1. State Key Laboratory of Crop Biology / Shandong Key Laboratory of Crop Biology / Agronomy College of Shandong Agricultural University, Tai’an 271018, China
  • Received:2011-12-29 Revised:2012-04-15 Online:2012-07-12 Published:2012-05-11
  • Contact: 万勇善, E-mail: yswan@sdau.edu.cn, Tel: 0538-8241540; 刘风珍, E-mail: liufz@sdau.edu.cn, Tel: 0538-8241540 E-mail:dongjinyu86@163.com

PDF

2239

Abstract: The peanut (Arachis hypogaea L.) cultivars are an allotetraploid consisting of A and B genomes. Δ9-Stearoyl-ACP desaturase (SAD) is a key enzyme that catalyzes the conversion of stearoyl-ACP to oleoyl-ACP, and finally controls the content of oleic acid as well as the proportion of saturated to unsaturated fatty acids. By using the primers based on the peanut cDNA sequence of SAD (AF172728), genomic DNAs were amplified from the wild diploid species A. duranensis and A. ipaensis, and from the cultivated accessions of peanut Fenghua 2, Shanhua 7 and Puyangdatuoyang, respectively. Two isoforms of the genomic SAD were identified and named as FhgSAD-1 and FhgSAD-2 from the cultivated peanut accessions. In addition, two isoforms of SAD cDNA were isolated and named as FhrSAD-1 and FhrSAD-2 from Fenghua 2. Comparison of the genomic sequences with cDNAs revealed that there were two introns in the SAD genomic sequences. Sequences alignment showed that the similarity between FhgSAD-1 and FhgSAD-2 in nucleotide level was over 97.5%, with 69 different sites in total, including 62 SNP sites and six variation sites of endonuclease recognition. The cDNA sequence similarity between FhrSAD-1 and FhrSAD-2 was 98.6%, with 98.9% nucleotides identity in coding region. Deduced amino acid sequences revealed that only one difference occurred in serine gathering area of 17PSSSSSSSSSSFSL30. gSAD-1 shared 99.9% nucleotide sequence homology with gSAD-A, while gSAD-2 was the same as gSAD-B. According to the phylogenetic tree, it is assumed that gSAD-1 and gSAD-2 may come from the A and B genome, respectively. The results revealed the characteristics of SAD sequences from different genomes of peanut, and provided important basis for exploring gene expression regulation and fatty acid component improvement in peanut seeds.

Key words: Peanut, Stearoyl-ACP desaturase (SAD), Sequence analysis

[1]Wan S-B(万书波). Peanut Quality (花生品质学). Beijing: China Agricultural Science and Technology Press, 2005. pp 2–10 (in Chinese)

[2]Chu Y, Holbrook C C, Ozias-Akins P. Two alleles of ahFAD2B control the the high oleic acid trait in cultivated peanut. Crop Sci, 2009, 49: 2029–2036

[3]Yukawa Y, Takaiwa F, Shojik K, Masuda K, Yamada K. Structure and expression of two seed-specific cDNA encoding stearoyl-acyl carrier protein desaturase from sesame, Sesamum indicum. Plant Cell Physiol, 1996, 37: 201–205

[4]Li X-D(李晓丹), Cao Y-L(曹应龙), Hu Y(胡亚), Xiao L(肖玲), Wu Y-H(武玉花), Wu G(吴刚), Lu C-M(卢长明). Fatty acid accumulation pattern in developing seeds of peanut. Chin J Oil Crop Sci (中国油料作物学报), 2009, 31(2): 157–162 (in Chinese with English abstract)

[5]Wendy C, Paolo L, Nunzia S, Monica D P, Paola S, Virginia C, Noreen M C, Alan M M, Peter M, Tony A K, Philip J D, Stefania G, Teodoro C. Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance. Transgenic Res, 2008, 17: 769–782

[6]Kachroo A, Shanklin J, Whittle E, Lapchyk L, Hildebrand D, Kachroo P. The Arabidopsis stearoyl-acyl carrier protein-desaturase family and the contribution of leaf isoforms to oleic acid synthesis. Plant Mol Biol, 2007, 63: 257–271

[7]Byfield G E, Xue H, Upchurch R G. Two genes from soybean encoding soluble Δ9-stearoyl-ACP desaturase. Crop Sci, 2006, 46: 840–846

[8]Ping Z, Joseph W B, Robert G U, Edward W, John S, Ralph E D. Mutations in a Δ9-Stearoyl-ACP-desaturase gene are associated with enhanced stearic acid levels in soybean seeds. Crop Sci, 2008, 48: 2305–2313

[9]Aardra K, John S, Edward W, Ludmila L, David H, Pradeep K. The Arabidopsis stearoyl-acyl carrier protein-desaturase family and the contribution of leaf isoforms to oleic acid synthesis. Plant Mol Biol, 2007, 63: 257–271

[10]Luo T, Deng W Y, Zeng J, Zhang F I. Cloning and characterization of a stearoyl-Acyl carrier protein desaturase gene from Cinnamomum longepaniculatum. Plant Mol Biol Rep, 2009, 27: 13–19

[11]Florin S, Yael B, Arnon B, Ilan H, Ran H. Identification and molecular characterization of homeologous Δ9-Stearoyl-acyl carrier protein desaturase3 genes from the allotetraploid peanut (Arachis hypogaea). Plant Mol Biol Rep, 2010, 29: 232–241

[12]Whittle E, Cahoon E B, Subrahmanyam S, Shanklin J. A multifunctional acyl-acyl carrier protein desaturase from Hedera helix L. (English ivy) can synthesize 16- and 18-carbon monoene and diene products. J Biol Chem, 2005, 280: 28169–28176

[13]Zaborowska Z, Starzycki M, Femiak I, Swiderski M, Legocki A B. Yellow lupine gene encoding stearoyl-ACP desaturase-organization, expression and potential application. Acta Biochimicn Polonicn, 2002, 49: 29–42

[14]Chen M N, Ren Z K, Chi X Y, Pan L J, Yu S L, Yang Q L. Isolation, characterization and expression analysis of stearoyl-ACP desaturase gene from Kosteletzkya virginica. Bioinform Biomed Engin (iCBBE), 2010, 4: 1–5

[15]Shah F H, Rashid O, San C T. Temporal regulation of two isoforms of cDNA clones encoding delta 9-stearoyl-ACP desaturase from oil palm (Elaies guineensis). Plant Sci, 2000, 152: 27–33

[16]Liu Q, Singh S P, Green A G. High-steric and oleic cottonseed oils produced by hairpin RNA-mediated post-transcriptional gene silencing. Plant Physiol, 2002, 129: 1–12

[17]Wendy C, Paolo L, Nunzia S, Monica D P, Paola S, Virginia C, Noreen M C, Alan M M, Peter M, Tony A K, Philip J D, Stefania G, Teodoro C. Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance. Transgenic Res, 2008, 17: 769–782

[18]Thompson G A, Scherer D E, Aken S F, Kenny J W, Young H L, Shintani D K, Kridl J C, Knauf V C. Primary structures of the precursor and mature forms of stearoyl-acyl carrier protein desaturase from safflower embryos and requirement of ferredoxin for enzyme activity. Proc Natl Acad Sci USA, 1991, 88: 2578–2582

[19]Zhang D-Q(张党权), Tan X-F(谭晓风), Chen H-P(陈鸿鹏), Zeng Y-L(曾艳玲), Jiang Y(蒋瑶), Li W(李魏), Hu F-M(胡芳名). Full-length cDNA cloning and bioinformatic analysis of Camellia oleifera SAD. Sci Silv Sin (林业科学), 2008, 44(2): 155–159 (in Chinese with English abstract)

[20]Dong S-Z(董胜张), Ye G-Y(叶恭银), Liu C-L(刘朝良). Research progress in molecular evolution of yolk proteins in insects. Acta Entomol Sin (昆虫学报), 2008, 51(11): 1 196–1 209 (in Chinese with English abstract)

[21]Sappington T W, Raikhel A S. Molecular characteristics of insect vitellogenin and vitellogenin receptors. Insect Biochem Mol Biol, 1998, 28: 277–300

[22]Lindqvist Y, Huang W, Schneider G, Shanklin J. Crystal structure of delta9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to other diiron proteins. EMBO J, 1996, 15: 4081–4092

[23]Krapovickas A, Gregory W C. Taxonomia del género Arachis (Leguminosae). Bonplandia, 1994, 8: 1–186

[24]Valls J F M, Simpson C E: New species of Arachis from Brazil, Paraguay, and Bolivia. Bonplandia, 2005, 14: 35–64

[25]Smartt J, Gregory W C, Gregory M P. The genomes of Arachis hypogaea L. cytogenetic studies of putative genome donor. Euphytica, 1978, 27: 665–675

[26]Moretzsohn M C, Hopkins M S, Mitchell S E, Kresovich S, Valls J F M, Ferreira M E. Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome. BMC Plant Biol, 2004, 4: 11

[27]Seijo G, Lavia G I, Fernández A, Krapovickas A, Ducasse D A, Bertioli D J, Moscone E A. Genomic relationships between the cultivated peanut (Arachis hypogaea Leguminosae) and its close relatives revealed by double GISH. Am J Bot, 2007, 94: 1963–1971

[28]Milla S R, Isleib T G, Stalker H T. Taxonomic relationships among Arachis sect. Arachis species as revealed by AFLP markers. Genome, 2005, 48: 1–11

[29]Fávero A P, Simpson C E, Valls J F M, Vello N A. Study of the evolution of cultivated peanut through crossability studies among Arachis ipaënsis, A. duranensis, and A. hypogaea. Crop Sci, 2006, 46: 1546–1552

[30]Jung S, Tate P L, Horn R, Kochert G, Moore K, Abbott A G. The phylogenetic relationship of possible progenitors of the cultivated peanut. J Hered, 2003, 94: 334–340
[1] Zheng Yu-Zhen, Qi Fei-Yan, Sun Zi-Qi, Liu Hua, Qin Li, Shi Lei, Wang Juan, Wang Meng-Meng, Han Suo-Yi, Xu Jing, Miao Li-Juan, Huang Bing-Yan, Dong Wen-Zhao, Zheng Zheng, Zhang Xin-You. QTL mapping of total very long-chain fatty acids and seven fatty acid components in peanut seeds [J]. Acta Agronomica Sinica, 2026, 52(6): 1646-1657.
[2] Lu Yi-Chu, Li Zhen-Ying, Mai Chun-Hai, Zhao Xiao-Rui, Wang Li-Xiang. Positively regulating role of the key evening complex gene AhLUX1 in peanut nodulation [J]. Acta Agronomica Sinica, 2026, 52(6): 1658-1668.
[3] Yu Tian-Yi, Wang Chun-Xiao, Xiao Li, Zhong Zhao-Di, Wang Xuan-Cang, Zhao Yong, Lu Ya, Wu Yue, Wu Zheng-Feng. Response of nitrogen accumulation, yield, and quality characteristics of peanut varieties with different nodulation traits to nitrogen fertilizer application rate [J]. Acta Agronomica Sinica, 2026, 52(3): 881-894.
[4] Zhang Sheng-Zhong, Li Guo-Wei, Ge Li-Jiang, Wang Fei-Fei, Hu Xiao-Hui, Miao Hua-Rong, Li Yan, Zhong Wen, Chen Jing. Screening and QTL mapping for mechanical shelling damage related traits in peanut [J]. Acta Agronomica Sinica, 2026, 52(2): 644-652.
[5] Wang Fei-Fei, Zhang Sheng-Zhong, Yang Gui-Hua, Miao Hua-Rong, Hu Xiao-Hui, Zhang Ze-Lin, Liu Sha-Sha, Qiao Li-Xian, Shan Shi-Hua, Chen Jing. Comprehensive evaluation of salt tolerance and identification of elite salt-tolerant germplasm in 331 peanut accessions at seedling stage [J]. Acta Agronomica Sinica, 2026, 52(1): 279-294.
[6] Jin Xin-Xin, Song Ya-Hui, Su Qiao, Yang Yong-Qing, Wang Jin. Growth and dry matter production characteristics of high-yielding, high-oil, and high oleic acid peanut varieties [J]. Acta Agronomica Sinica, 2026, 52(1): 191-201.
[7] Chi Xiao-Yuan, Liu Qing, Zhang Jun, Zhao Xu-Hong, Li Mei, Yu Tian-Yi, Pan Li-Juan, Xu Jing, Jiang Xiao, Yin Xiang-Zhen, Ma Jun-Qing, Chen Na. Field evaluation of salt-alkaline tolerance and trait correlation analysis in different peanut varieties (lines) [J]. Acta Agronomica Sinica, 2026, 52(1): 85-98.
[8] Sun Chen-Shuo, Zhang Yue, Tian Ze-Kai, Yan Li-Ying, Kang Yan-Ping, Chen Yu-Ning, Wang Xin, Huai Dong-Xin, Wang Qian-Qian, Jiang Hui-Fang, Luo Huai-Yong, Huang Li, Liao Bo-Shou, Wang Zhi-Hui, Lei Yong. Genetic differentiation of peg strength and analysis of major influencing factors in peanut germplasm [J]. Acta Agronomica Sinica, 2026, 52(1): 118-130.
[9] WAN Shu-Bo, ZHANG Jia-Lei, GAO Hua-Xin, WANG Cai-Bin. Advances and prospects of high-yield peanut cultivation in China [J]. Acta Agronomica Sinica, 2025, 51(7): 1703-1711.
[10] GUO Teng-Da, CUI Meng-Jie, CHEN Lin-Jie, HAN Suo-Yi, GUO Jing-Kun, WU Chen-Di, FU Liu-Yang, HUANG Bing-Yan, DONG Wen-Zhao, ZHANG Xin-You. Cloning and expression analysis of the phosphatidylinositol transfer protein AhSFH gene in peanuts responsive to Aspergillus flavus infection [J]. Acta Agronomica Sinica, 2025, 51(6): 1489-1500.
[11] LI Wen-Jia, LIAO Yong-Jun, HUANG Lu, LU Qing, LI Shao-Xiong, CHEN Xiao-Ping, JIN Jing-Wei, WANG Run-Feng. Genome-wide associate analysis of flowering traits and identification of candidate genes in peanut [J]. Acta Agronomica Sinica, 2025, 51(5): 1400-1408.
[12] LIN Wei-Jin, GUO Ze-Jia, LIU Hao, LI Hai-Fen, WANG Run-Feng, HUANG Lu, YU Qian-Xia, CHEN Xiao-Ping, HONG Yan-Bin, LI Shao-Xiong, LU Qing. QTL mapping and candidate gene analysis of peanut pod yield-related traits [J]. Acta Agronomica Sinica, 2025, 51(4): 969-981.
[13] CHI Xiao-Yuan, BI Jing-Nan, ZHAO Jian-Xin, CHEN Na, PAN Li-Juan, JIANG Xiao, YIN Xiang-Zhen, ZHAO Xu-Hong, MA Jun-Qing, XU Jing. Evaluation of mechanical properties of peanut pods and screening of early maturing germplasm [J]. Acta Agronomica Sinica, 2025, 51(4): 943-957.
[14] JIN Gao-Rui, WU Xiao-Li, DENG Li, CHEN Yu-Ning, YU Bo-Lun, GUO Jian-Bin, DING Ying-Bin, LIU Nian, LUO Huai-Yong, CHEN Wei-Gang, HUANG Li, ZHOU Xiao-Jing, HUAI Dong-Xin, TAN Jia-Zhuang, JIANG Hui-Fang, REN Li, LEI Yong, LIAO Bo-Shou. Development and characterization of novel peanut genetic stocks with high oleic acid and enhanced resistance both to Aspergillus flavus infection and aflatoxin production [J]. Acta Agronomica Sinica, 2025, 51(3): 687-895.
[15] JIN Xin-Xin, SONG Ya-Hui, SU Qiao, YANG Yong-Qing, LI Yu-Rong, WANG Jin. Identification and comprehensive evaluation of drought resistance in high oleic acid Jihua peanut varieties [J]. Acta Agronomica Sinica, 2025, 51(3): 797-811.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd