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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (11): 1949-1959.doi: 10.3724/SP.J.1006.2012.01949

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Screening and Evaluation of Heat Tolerance of Introgression Lines with Japonica Chaoyou 1 Background

MENG Li-Jun1,2,MA Xiu-Fang3,**,TANG Zhi-Qiang3,SHEN Feng3,CUI Yan-Ru1,CAI Lu1,CHEN Kai1,XU Jian-Long1,*,LI Zhi-Kang1,*   

  1. 1 Shenyang Agricultural University / Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Shenyang 110161, China;
    2 Institute of Crop Sciences / National Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Rice Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110101, China
  • Received:2012-03-29 Revised:2012-06-10 Online:2012-11-12 Published:2012-09-01
  • Contact: 徐建龙, E-mail: xujlcaas@yahoo.com.cn; 黎志康, E-mail: zhkli1953@126.com

Abstract:

Global climatic warming inevitably affects rice production. Nine BC2F4 backcross introgression populations derived from crosses between a japonica variety Chaoyou1 (CY1) as the recurrent parent (RP) and nine donor parents collected from different countries were screened for heat tolerance (HT) at flowering stage. A total of 124 introgression lines (ILs) were selected based on seed setting rate in single plant under high temperature (38℃). The results showed that: introgression lines (ILs) had better HT than Chaoyou1, and the frequency of plants with HT derived from the ILs with donors of japonica was higher than that with donors of indica, demonstrating the potential of using genes from japonica varieties to improve HT of breeding line. Progeny testing of the 124 ILs under heat stress and normal condition revealed a high selection efficiency of 64.5% for HT and 80 ILs had higher SF than RP. The 124 selected ILs presented wide segregations for the tested traits in replicated experiments under stress and normal conditions and allowed us to identify eight promising ILs that had greatly improved HT and higher yield than CY1. HT-tolerant plants selected from three pyramiding populations had an average SF of more than 80%, which was significantly higher than that of RP and the highest pyramiding parent. From them, 106 plants with significantly better HT than pyramiding parent were selected, showing ideal pyramiding effect on HT. Detailed comparisons between the ILs and CY1 for yield performances and related traits under heat stress and non-stress conditions provided useful information and better understanding in donor selection, selection efficiency and screening method for HT in the BC breeding process. The selected lines with HT will become valuable materials for gene mining and molecular breeding for improvement of HT by high efficient pyramiding of favorable genes.

Key words: Rice (Oryza sativa L.), Heat tolerance, High temperature stress, Introgression line, Pyramiding breeding

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