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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (09): 1374-1380.doi: 10.3724/SP.J.1006.2016.01374


Effect of High Temperature Stress on Bt Insecticidal Protein Content and Nitrogen Metabolism of Square in Bt Cotton

HENG Li,HU Da-Peng,WANG Gui-Xia,LYU Chun-Hua,ZHANG Xiang,CHEN Yuan,CHEN De-Hua*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / College of Agriculture, Yangzhou University, Yangzhou 225009, China?
  • Received:2016-03-14 Revised:2016-06-20 Online:2016-09-12 Published:2016-06-27
  • Contact: 陈德华, E-mail: cdh@yzu.edu.cn, Tel: 18051058960 E-mail:917103441@qq.com
  • Supported by:

    The research was supported by the National Natural Science Foundation of China (31171479, 31301263, 31471435), the Ministry of Education Research Fund for the Doctoral Program (20113250110001), Jiangsu “Three-innovation” Agricultural Project [SXGC(2014)317], and the Development Plan of Higher Education Advantaged Discipline in Jiangsu Province.


To explore the reason for low insect resistance under high temperature climate in Bt cotton production, we selected two Bt cotton cultivars as experimental material with treatments of 38°C for three, five and seven days in 2011, and 38°C/25°C at day /night for four, seven and ten days, respectively at the artificial climate chamber in 2012. The results showed that the Bt insecticidal protein contents declined within seven days under 38℃, with the largest reduction occurred within three days. Compared with control, the square Bt insecticidal protein contents decreased by 18.71% and 26.54% for cultivar Sikang3 and Sikang1, respectively. Under 38°C/25°C at day /night , the square Bt insecticidal protein contents had no obvious reduction within four days, and significant reduced after seven days with the reduction of 11.32% for SK-3and 14.18% for SK-1. Under the treatment conditions the soluble protein contents, glutamate pyruvate transaminase (GPT) activities reduced, but the free amino acid contents, protease activities increased. There existed significant negative correlation of insecticidal protein content with free amino acid contents, and protease activities ; and significantly positive correlation of insecticidal protein content with soluble protein contents, and GPT activities under high temperature stresses. Therefore, the reduced synthesis and the enhanced degradation for protein in the square under the high temperature condition resulted in the decrease of soluble protein content, including Bt insecticidal protein content. The larger reductions of the square Bt insecticidal protein content and nitrogen metabolic strength were detected in cultivar Sikang1 as confound with cultivar SK-3 under the high temperature treatments.

Key words: Bt cotton, Square, Bt insecticidal protein, Nitrogen metabolism, High temperature stress

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