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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (3): 440-447.doi: 10.3724/SP.J.1006.2020.94080


Recovery characteristics of Bt insecticidal protein and relative physiological mechanisms after high temperature stress termination in square of Bt cotton

Zhen-Yu LIU,Gui-Xia WANG,Li-Nan LI,Ze-Zhou CAI,Pan-Pan LIANG,Xin-Ling WU,Xiang ZHANG,De-Hua CHEN()   

  1. Jiangsu Key Laboratory of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China
  • Received:2019-05-27 Accepted:2019-09-26 Online:2020-03-12 Published:2019-10-14
  • Contact: De-Hua CHEN E-mail:cdh@yzu.edu.cn
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31671613);the China Agriculture Research System (Cotton Post Expert Project)(CARS-18-18);the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)


The experiments were conducted at Key Laboratory of Genetics and Physiology of Yangzhou University. The conventional cultivar Sikang-1 and hybrid cultivar Sikang-3 were used as the experimental materials. The potted cotton at squaring stage was moved to artificial climatic chamber with 38℃ for 72 h , and 38℃ for 96 h respectively in 2017 and 2018 cotton growth season, and moved to natural conditions, then the squares were collected to measure the Bt protein concentration and nitrogen metabolic physiology at 0, 12, 24, 48, 72, 96 hours respectively after the high temperature termination. The square Bt insecticidal protein contents were able to recover to the corresponding control level, the time for the recovery was in need of longer as the stressed high temperature period increased. In 72 h high temperature treatment, the square Bt insecticidal protein content could recover to the corresponding control level at 72 h for Sikang-1 and at 48 h for Sikang-3. In 96 h high temperature treatment, the square Bt insecticidal protein content could recover to the corresponding control level at 96 h for Sikang-1 and at 72 h for Sikang-3. There were significantly positive correlations of Bt toxin content with soluble protein content, glutamate pyruvate transaminase activity and glutamate oxaloacetate transaminase activity (the correlation coefficients were 0.964 **, 0.981 **, and 0.971 **, respectively), and significantly negative correlations of Bt toxin content with free amino acid contents, and activitied of protease and peptidase (the correlation coefficients were -0.894 **, -0.912 **, and -0.834 **, respectively). Therefore, the recovery degree of Bt insecticidal protein content of cotton square can be predicted according to the duration of high temperature stress, reasonably controlling Helicoverpa armigera and related pests in production.

Key words: Bt cotton, square, high temperature stress, Bt protein, nitrogen metabolism

Fig. 1

Changes of insecticidal protein contents in flower buds of Bt cotton after 72 h high temperature stress termination Bt toxin contents at the recovery time with different lowercase letters are significantly different from the control at P < 0.05 under the same high temperature duration for the same cultivar. T: reatments for 72 hours; CK: control."

Fig. 2

Changes of insecticidal protein contents in flower buds of Bt cotton after 96 h high temperature stress termination Bt toxin contents at the recovery time with different lowercase letters are significantly different from the control at P < 0.05 under the same high temperature duration for the same cultivar. T: treatments for 96 hours; CK: control."

Table 1

Changes of square soluble protein and free amino acid contents with time after high temperature stress termination"

Tested index
恢复时间 Recovery time
0 h 12 h 24 h 48 h 72 h 96 h
Soluble protein content
(mg g-1 FM)
SK-1 CK 10.19 a 10.25 a 10.25 a 10.19 a 10.16 a 10.26 a
38℃-72 h 4.77 d 5.12 d 6.45 c 8.56 b 10.36 a 10.36 a
38℃-96 h 4.15 e 4.44 e 5.43 d 6.86 c 8.47 b 10.09 a
SK-3 CK 12.10 a 12.20 a 12.27 a 12.25 a 12.18 a 12.24 a
38℃-72 h 6.26 d 7.42 c 9.17 b 12.05 a 12.23 a 12.27 a
38℃-96 h 5.93 e 6.80 d 7.88 c 10.89 a 12.02 a 12.23 a
Free amino acid content
(μmol g-1 FM)
SK-1 CK 255.21 a 250.16 a 249.88 a 251.92 a 253.55 a 249.60 a
38℃-72 h 747.74 a 693.73 b 626.64 c 476.67 d 240.14 d 243.28 d
38℃-96 h 974.31 a 923.16 b 854.25 c 689.28 d 500.86 e 252.38 e
SK-3 CK 277.06 a 273.20 a 281.32 a 277.44 a 275.88 a 272.99 a
38℃-72 h 681.54 a 607.52 b 520.30 c 286.08 d 279.63 d 272.75 d
38℃-96 h 905.24 a 829.97 b 742.43 c 346.71 d 273.32 e 274.59 e

Table 2

Changes of square GOT and GPT activities with time after high temperature stress termination"

Tested index
恢复时间 Recovery time
0 h 12 h 24 h 48 h 72 h 96 h
(μmol g-1 h-1)
SK-1 CK 12.71 a 12.79 a 12.69 a 12.95 a 12.40 a 12.49 a
38℃-72 h 3.79 e 4.62 d 6.05 c 8.59 b 12.77 a 12.61 a
38℃-96 h 2.20 f 2.84 e 3.99 d 6.52 c 9.40 b 13.59 a
SK-3 CK 15.37 a 15.25 a 15.34 a 15.24 a 15.34 a 15.19 a
38℃-72 h 6.36 d 8.51 c 11.76 b 15.84 a 15.62 a 15.72 a
38℃-96 h 4.31 e 5.77 d 7.71 c 13.53 a 15.93 a 15.82 a
(μmol g-1 h-1)
SK-1 CK 14.19 a 14.24 a 14.35 a 14.19 a 14.25 a 14.30 a
38℃-72 h 4.73 e 5.78 d 7.14 c 9.72 b 14.35 a 14.61 a
38℃-96 h 3.30 f 4.09 e 5.08 d 7.42 c 10.54 b 14.21 a
SK-3 CK 17.05 a 16.63 a 17.57 a 17.07 a 17.60 a 17.34 a
38℃-72 h 6.45 d 8.88 c 11.59 b 17.57 a 17.30 a 17.25 a
38℃-96 h 4.81 e 5.86 d 8.17 c 14.64 a 17.20 a 17.49 a

Table 3

Changes of square protease and peptidase activities with time after high temperature stress termination"

Tested index
恢复时间 Recovery time
0 h 12 h 24 h 48 h 72 h 96 h
Protease activity
(μg g-1 h-1)
SK-1 CK 34.62 a 34.08 a 34.88 a 33.86 a 35.07 a 34.58 a
38℃-72 h 125.79 a 114.46 b 100.05 c 73.23 d 35.43 d 34.88 d
38℃-96 h 153.95 a 145.35 b 131.47 c 106.43 d 76.00 e 35.35 e
SK-3 CK 39.84 a 40.76 a 40.98 a 40.67 a 39.74 a 40.19 a
38℃-72 h 114.88 a 99.46 b 83.75 c 39.08 d 41.16 d 39.94 d
38℃-96 h 141.91 a 122.30 b 105.78 c 62.09 d 43.01 e 41.49 e
Peptidase activity
(μmol g-1 h-1)
SK-1 CK 1.28 a 1.28 a 1.29 a 1.27 a 1.28 a 1.27 a
38℃-72 h 3.43 a 3.24 a 3.06 ab 2.46 b 1.26 d 1.27 d
38℃-96 h 4.07 a 3.91 b 3.72 c 3.15 d 2.50 e 1.30 f
SK-3 CK 1.64 a 1.61 a 1.62 a 1.62 a 1.61 a 1.60 a
38℃-72 h 3.20 a 2.77 b 2.32 c 1.56 d 1.62 d 1.59 d
38℃-96 h 3.79 a 3.48 b 3.11 c 2.07 d 1.59 e 1.59 e
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