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Table of Content

    12 November 2012, Volume 38 Issue 11
    • CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
      Screening and Evaluation of Heat Tolerance of Introgression Lines with Japonica Chaoyou 1 Background
      MENG Li-Jun,MA Xiu-Fang,TANG Zhi-Qiang,SHEN Feng,CUI Yan-Ru,CAI Lu,CHEN Kai,XU Jian-Long,LI Zhi-Kang
      Acta Agron Sin. 2012, 38(11):  1949-1959.  doi:10.3724/SP.J.1006.2012.01949
      Abstract ( 1946 )   PDF (209KB) ( 1293 )   Save
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      Global climatic warming inevitably affects rice production. Nine BC2F4 backcross introgression populations derived from crosses between a japonica variety Chaoyou1 (CY1) as the recurrent parent (RP) and nine donor parents collected from different countries were screened for heat tolerance (HT) at flowering stage. A total of 124 introgression lines (ILs) were selected based on seed setting rate in single plant under high temperature (38℃). The results showed that: introgression lines (ILs) had better HT than Chaoyou1, and the frequency of plants with HT derived from the ILs with donors of japonica was higher than that with donors of indica, demonstrating the potential of using genes from japonica varieties to improve HT of breeding line. Progeny testing of the 124 ILs under heat stress and normal condition revealed a high selection efficiency of 64.5% for HT and 80 ILs had higher SF than RP. The 124 selected ILs presented wide segregations for the tested traits in replicated experiments under stress and normal conditions and allowed us to identify eight promising ILs that had greatly improved HT and higher yield than CY1. HT-tolerant plants selected from three pyramiding populations had an average SF of more than 80%, which was significantly higher than that of RP and the highest pyramiding parent. From them, 106 plants with significantly better HT than pyramiding parent were selected, showing ideal pyramiding effect on HT. Detailed comparisons between the ILs and CY1 for yield performances and related traits under heat stress and non-stress conditions provided useful information and better understanding in donor selection, selection efficiency and screening method for HT in the BC breeding process. The selected lines with HT will become valuable materials for gene mining and molecular breeding for improvement of HT by high efficient pyramiding of favorable genes.

      Development and Validation of CAPS Markers for Marker-Assisted Selection of Rice Blast Resistance Gene Pi25
      WANG Hui-Mei,CHEN Jie,SHI Yong-Feng,PAN Gang,SHEN Hai-Chao,WU Jian-Li
      Acta Agron Sin. 2012, 38(11):  1960-1968.  doi:10.3724/SP.J.1006.2012.01960
      Abstract ( 2254 )   PDF (871KB) ( 1494 )   Save
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      To promote the application of rice blast resistance gene Pi25 in rice breeding programs, we developed four sets of gene-specific CAPS markers (CAP1/Hinc II, CAP3/Bgl II, CAP3/Nde I, and CAP3/Hpy 99I) based on the coding sequences of the locus. One hundred and sixty-nine rice accessions, 98 recombinant inbred lines (RILs) and 217 transgenic plants were used for the validation of the markers. The results showed that all the four sets of markers were able to accurately and efficiently detect the Pi25/pi25 locus, CAP1/Hinc II and CAP3/Hpy 99I could digest specifically the dominant allele Pi25 while CAP3/Bgl I and CAP3/Nde I were able to digest specifically the recessive allele pi25. RILs and transgenic lines carrying Pi25 allele were resistant to the blast isolate JS001-20 while the lines carrying pi25 allele were susceptible, indicating a perfect detection of the target locus by the CAPS markers. In addition, a low frequency (1.2%) of the dominant allele was detected in the germplasm collections, indicating this gene has not been fully utilized in rice breeding programs in China. Markers CAP1/Hinc II and CAP3/Hpy 99I are recommended and will be useful for the improvement of blast resistance, especially for the early-season indica rice.

      Analysis of Resistance to Heterodera filipjevi in Triticum aestivumDasypyrum villosum Germplasm
      ZHANG Jia-Jia,YUAN Hong-Xia,ZHANG Rui-Qi,XING Xiao-Ping,DAI Jun-Li,NIU Ji-Shan,LI Hong-Lian,CHEN Pei-Du
      Acta Agron Sin. 2012, 38(11):  1969-1976.  doi:10.3724/SP.J.1006.2012.01969
      Abstract ( 1770 )   PDF (252KB) ( 1241 )   Save
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      Heterodera filipjevi is a new pathogenic species of cereal cyst nematode (CCN) in China, which has a potential threat to wheat production security. Wheat relatives are important resources of multiple resistance genes to many biotic and abiotic stresses, including CNN resistance genes. Using relative resistance index (RRI) as the evaluation indicator, we screened resistant resources against H. filipjevi from 20 wheat relatives after artificial inoculation, and found Dasypyrum villosum highly and stably resistant to H. filipjevi Xuchang population. Three sets of common wheat (Triticum aestivum L. cv. Chinese Spring) chromosome addition lines with individual D. villosum chromosome 1V to 7V, as well as 6VS translocation lines from different origins, were evaluated for CCN resistance. The Triticum aestivumD. villosum 6V chromosome addition line showed high resistance to CCN with RRI higher than 0.90 in all sets of addition lines; however, the translocation lines 6VS were susceptible to CNN with RRI ranging from 0.43 to 0.51. Therefore, the CCN resistance gene is most probably located on chromosome 6VL.

      Maturity Group Classification of Check Varieties in National Soybean Uniform Trials of China
      WU Cun-Xiang1,2, LI Ji-Cun,SHA Ai-Hua,ZENG Hai-Yan,SUN Shi,YANG Guang-Ming,ZHOU Xin-An,CHANG Ru-Zhen,NIAN Hai,HAN Tian-Fu
      Acta Agron Sin. 2012, 38(11):  1977-1987.  doi:10.3724/SP.J.1006.2012.01977
      Abstract ( 2463 )   PDF (162KB) ( 1429 )   Save
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      Maturity Group (MG) is a widely-used system for soybean varieties classification in the world. However, it has not been adopted yet in China. In the current studies, we identified the MG catagories for 19 check varieties from the National Soybean Uniform Trials of China by comparing the check varieties with 38 MG standard varieties from the North America, which covered MG000–MGVIII. The check varieties from 16 trial groups represented most of the released soybean varieties in China. All standard and check varieties were sown in spring of Beijing and Wuhan, and the supplementary tests using local varieties were conducted in other 18 sites across the country. The results showed that the MGs of check varieties in the National Soybean Uniform Trials ranged from MG0 to MGVI. Some varieties from different trial groups could be classified into the same MGs, for example, MGIII included the varieties from the Late Group of the Northern Spring Planting Soybean Region, Northwest Spring Planting Soybean Region, all three (north, mid and south) zones of Huanghuaihai Summer Planting Soybean Region, and Southwest Mountainous Spring Planting Soybean Region; the varieties from Yangtze River Spring Planting Soybean Region and the Tropical and Subtropical Summer Planting Soybean Regions were classified into MGV and MGVI. Fudou 301 and Quandou 7, check vrieties from the Tropical and Subtropical Spring Planting Soybean Regions, belonged to MGII and MGIV, respectively. Based on the MG classification and other factors, the authors suggested that the Huanghuaihai Summer Planting Soybean Region can be changed to two (north and south) zones from the current three (north, mid, and south) zones, and the regionization of National Soybean Uniform trials in South China should also be modified. Late Maturity Group of the Northern Spring Planting Soybean Region and the Northwest Spring Planting Soybean Region, both belong to MGIII, should be separated because they are different ecotypes in drought tolerance. The results of this experiment pave the way for establishing the MG system based on biological identities of varieties in order to classify soybean varieties and regionalize the soybean production regions in China.

      Preliminary Functional Analysis of a Rice Leaf Senescence Up-Regulated Gene
      SUN Bo,ZHOU Yong,LIN Yong-Jun
      Acta Agron Sin. 2012, 38(11):  1988-1996.  doi:10.3724/SP.J.1006.2012.01988
      Abstract ( 2409 )   PDF (317KB) ( 1622 )   Save
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      Leaf senescence is the last stage in leaf development. It has important theoretical and practical significance to study the mechanism on rice leaf senescence and the find out a way to purposefully control or delay leaf senececne. In this study, we identified a rice leaf senescence up-regulated gene A12 (LOC_Os 12g33120) according to the leaf senescence gene expression microarray data. The analysis from the rice gene expression profile database in whole growth period and qRT-PCR result of A12 expression in flag leaves at different stages after heading further confirmed that A12 was a leaf senescence up-regulated gene. Bioinformatic analysis found many phytohormone-responsive cis-acting elements in A12 promoter. Quantitative real-time PCR analysis indicated the A12 gene were up-regulated by JA and KT treatments, but not apparently induced by BR, GA, IAA, and ABA. Research on the rice T-DNA insertion mutant of A12 found that loss of function of this gene led to premature senescence of flag leaf. These results lay a foundation for the further functional analysis of this gene.

      Association between Starch Pasting Properties and DArT Markers in Common Wheat
      YU Hai-Xia,TIAN Ji-Chun
      Acta Agron Sin. 2012, 38(11):  1997-2006.  doi:10.3724/SP.J.1006.2012.01997
      Abstract ( 1748 )   PDF (313KB) ( 1123 )   Save
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      Starch pasting properties are important indicators in the evaluation of processing quality and quality breeding in wheat (Triticum aestivum L.), which are commonly represented by RVA parameters including peak viscosity, trough viscosity, breakdown, final viscosity, setback, and pasting temperature. These RVA parameters of Aimengniu and its derived progenies planted in five environments in two locations from 2007 to 2010 were determined, and the association between the RVA parameters and 971 DArT (Diversity Array Technology) markers were analyzed using mixed linear model. Seventy DArT markers distributed on 19 chromosomes were significantly associated with the six RVA parameters (P ≤ 0.001, R2 = 0.2–23.3%). The markers on chromosomes 2A, 2B, and 2D were identified to have large effects and associated with several RVA parameters. This result provides an important information for marker-assisted selection on starch pasting properties in wheat.

      Epistatic and Additive Effects of QTL for Yield-Related Traits Using Single Segment Substitution Lines in Rice (Oryza sativa L.)
      ZHAO Fang-Ming,ZHANG Gui-Quan,ZENG Rui-Zhen,YANG Zheng-Lin,LING Ying-Hua,SANG Xian-Chun,HE Guang-Hua
      Acta Agron Sin. 2012, 38(11):  2007-2014.  doi:10.3724/SP.J.1006.2012.02007
      Abstract ( 1946 )   PDF (232KB) ( 1332 )   Save
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      Yield-related traits such as panicle number per plant, thousand grain weight, number of grains per panicle, number of spikelets per panicle and seed setting rate are important agronomic traits in rice. Understanding additive and epistatic effects of QTL for yield-related traits are important to increase rice yield using method of pyramiding breeding with molecular marker assisted selection. In this paper, additive and epistatic effects of QTLs for rice yield-related traits were analyzed using 16 single segment substitution lines (SSSL) and 15 double segment substitution lines (DSSL). A total of thirteen QTLs for yield-related traits were identified on the chromosomes 2, 3, 4, 7, and 10 respectively, containing one for grain yield per plant (GY), one for panicle number (PN), four for thousand grain weight (TGW), four for number of grains per panicle (NGP), two for number of spikelets per panicle (NSP) and one for seed setting rate (SSR). Furthermore, twelve pairs of digenic interactions were detected for yield-related traits. The results showed that pyramiding two QTLs with positive effects (or two QTLs with negative effects) often results in negative epistatic effects (or positive epistatic effects) in DSSL. Whether larger or smaller value of yield-related traits is produced lies on the difference between the absolute value of genetic effect (algebraic sum of additive and epistatic effects) in the DSSL and the largest value of additive effect in the SSSL. These results are important to improve yield by pyramiding favorable QTLs for yield-related traits.

      Inheritance of Sterility in Genic Male Sterile Line (20118A) and Marker-Assisted Selection in Hybrid Breeding of Brassica napus L.
      REN Meng-Yang,NI Xi-Yuan,WANG Hao,CHEN Fei,TIAN Jian-Hua,HUANG Ji-Xiang,LI Dian-Rong,ZHAO Jian-Yi
      Acta Agron Sin. 2012, 38(11):  2015-2023.  doi:10.3724/SP.J.1006.2012.02015
      Abstract ( 1848 )   PDF (296KB) ( 1311 )   Save
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      In the present study, twenty varieties/lines of Brassica napus were test-crossed with sterile lines 20118A, and its temporary maintainer 20118A-TAM. Both traditional genetic analysis and molecular marker assisted technology were employed to confirm their genetic model of sterility with two gene control system, to check the allelic distributions among normal rapeseed varieties or lines. Furthermore, the efficiency and accuracy of marker-assisted selection (MAS) for two-type line and temporary maintainers based on co-dominant markers were also investigated. The results showed that the segregation proportion of male fertile to sterile plants in F2 progenies from six varieties (lines) crossed with 20118A fitted mendelian segregation (3:1 and 13:3), and that with 20118A-TAM showed either 13:3 or full male fertile, indicating that the sterility of 20118Ais controlled by one recessive sterile gene interacting with a recessive epistatic suppression gene. In addition, a reverse validation approach based on Bnms3 and Bnrf linked marker assisted selection was used to further confirm the two gene control system. From a total of 1059 F2 plants, 70, 69 and 135 individuals carrying temporary maintainer(ms3ms3rfrf), homozygous sterile (ms3ms3RfRf) and fertile (Ms3ms3RfRf) marker genotypes were screened out, respectively, which also fitted the Mendelian segregation proportions of two gene model (1/16, 1/16, and 1/8). After test-crossing with known homozygous sterile plants or temporary maintainers or one another among marker genotypes, higher than 95% of lines were approved to be the expected genotypes. Finally, according to the information from 20 testcross cultivars/lines, only two alleles of Rf and rf were observed in BnRf locus, implying the third allele naturally existed very few, if any. Therefore, for practical breeding purpose, a marker assisted selection strategy simply based on BnRf/rf and BnMs3/ms3linked co-dominant markers is proposed.

      Cloning and Expression Analysis of ATP-Citrate Lyase Genes from Sugarcane
      LI Chang-Ning,NONG Qian,TAN Qin-Liang,SRIVASTAVA Manoj Kumar,YANG Li-Tao,LI Yang-Rui
      Acta Agron Sin. 2012, 38(11):  2024-2033.  doi:10.3724/SP.J.1006.2012.02024
      Abstract ( 1728 )   PDF (725KB) ( 1296 )   Save
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      Acetyl-CoA plays an important role in the cytosol of plant cells for the synthesis of a diverse set of phytochemicals, and the cytosolic acetyl-CoA is from the reaction of citrate and CoA catalyzed by ATP-citrate lyase (ACL) coupled with the hydrolysis of ATP. In this research, two genes encoding two distinct subunits of ACL were identified from sugarcane (Saccharum officinarum) by RACE and insilico cloning technology, named SoACLA-1 and SoACLB-1, which contained 1 272 and 1 828 bp open reading frames, and encoded 423 and 608 amino acids, respectively. Sequence analysis indicated that both amino acids sequences showed high homology with ACLs from other species and were close clustered with ACLs from gramineous plants in the phylogenetic tree constructed by MEGA5.0 software using a Neighborhood-Joining bootstrap method. Both sequences showed high conservation in the ATP-grasp domain, citrate binding site, active binding site of histidine phosphorylated by ATP, potential ATP-binding, phosphorylated site and CoA-binding site when compared with ACLs from other species. Quantitative real-time PCR results indicated that SoACLA-1 and SoACLB-1 were both induced by the treatment of control+ABA, water stress, water stress +ABA, and their expression levels were higher in leaves than in roots, with the highest express level in water stress treatment, SoACLA-1 and SoACLB-1 showed a synergetic expression pattern. The expression of SoACLA-1 and SoACLB-1 was close correlated with the ABA and ROS contents, indicating that ACL maybe involved in stress responsive metabolic process triggered by ABA in plants.

      Molecular Cloning and Development of a Functional Marker of TaABP1-D Gene Associated with Plant Height in Bread Wheat
      QIAO Lin-Yi,ZHANG Lei,ZHANG Wen-Ping,ZHAO Guang-Yao,WANG Xi,JIA Ji-Zeng
      Acta Agron Sin. 2012, 38(11):  2034-2041.  doi:10.3724/SP.J.1006.2012.02034
      Abstract ( 2215 )   PDF (480KB) ( 1437 )   Save
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      AUXIN BINDING PROTEIN1 (ABP1) is one of the ?rst characterized proteins that binds auxin. It ful?lls the criteria for an auxin receptor and plays an important role in auxin responses on the plasma membrane. In this study, the genomic sequence of a novel gene, designated TaABP1, isolated from the hexaploid wheat (Triticum aestivum L.) Chinese Spring genome databases. The result of chromosomal location showed that TaABP1 was on homoelogous group 5 of chromosomes in wheat. Then TaABP1-D was cloned in Chinese Spring. Sequence analysis showed that the complete open reading frame (ORF) of TaABP1-D was 1887 bp in length, encoding a putative protein composed of 205 amino acids with theendoplasmic reticulum retention sequence Lys-Asp-Glu-Leu (KDEL) and box regions. The expression of TaABP1-D was detected in root, basal stem, upper stem, and leaf tip of wheat at jointing stage with the expression levels as leaf tip > upper stems > root > base stem. Sequence alignment proved that TaABP1-D shared high homology with rice OsABP1 (82% protein sequence identity) and other plant ABPs reported. Using a population of recombinant inbred lines from the cross between W7984 and Opata85, an SSR maker was developed based on (GT)6/5 divergence in the upstream sequence of TaABP1-D. This marker was proved to be highly correlated with plant height, and the phenotypic variation explained was 9.7%. The W7984 type allelic variation for tall plant was specific in wild varieties, and eliminated in cultivated varieties. This indicates that the favorable variation of TaABP1-D has been fixed in cultivated varieties of wheat, and a bottleneck effect might occur during the domestication of wheat.

      Ectopic Expression of TaYAB2, a Member of YABBY Gene Family in Wheat, Causes Partial Abaxialization of Adaxial Epidermises of Leaves in Arabidopsis
      ZHAO Xiang-Yu,XIE Hong-Tao,CHEN Xiang-Bin,WANG Shuai-Shuai,ZHANG Xian-Sheng
      Acta Agron Sin. 2012, 38(11):  2042-2051.  doi:10.3724/SP.J.1006.2012.02042
      Abstract ( 2135 )   PDF (634KB) ( 1611 )   Save
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      Adaxial-abaxial polarity is an important feature of lateral organs. To study the molecular mechanism of polarity establishment, we isolated a YABBY gene, designated TaYAB2, from the young leaves of wheat. TaYAB2 has a zinc finger-like domain in the N terminus and a YABBY domain in the C terminus. Sequence comparison showed that TaYAB2 is a putative member of the YABBY gene family in wheat. Further expression analysis indicated that TaYAB2 was widely expressed in the lateral organs of wheat. Ectopic expression of TaYAB2 inArabidopsis caused the partial abaxialization of the adaxial epidermises of leaves with the promotion of the transcript levels of abaxial identity genes, for example, FIL/YAB1, YAB3, and KAN1. These results indicate that TaYAB2 affects the establishment of adaxial-abaxial polarity when ectopically expressed in Arabidopsis.

      Differential Gene Expression Profiles in Developing Seeds of Brassica napus L. under Different Nitrogen Application Levels
      YAN Gui-Xin,CHEN Bi-Yun,XU Kun,GAO Gui-Zhen,Lü Pei-Jun,WU Xiao-Ming,LI Feng,LI Jun
      Acta Agron Sin. 2012, 38(11):  2052-2060.  doi:10.3724/SP.J.1006.2012.02052
      Abstract ( 1679 )   PDF (334KB) ( 1307 )   Save
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      Nitrogen is one of the major fertilizers to Brassica napus L., it has concluded that increasing nitrogen would raise seed yield, enhance protein abundance but decrease oil content. Up-to-date, little is known on screening nitrogen-tolerant B. napus and identifying nitrogen-response genes and their networks. In the paper, we designed four nitrogen application levels (0, 90, 180, and 270 kg ha–1) using Brassica napus accessions Zhongshuang 11 and Parter (from Germany) with randomized block design. The results indicated that the seed protein content increased whereas oil content decreased with increasing nitrogen levels. There existed variance in protein and oil contents between Zhongshuang 11 and Parter. The comparison of 25 DAP seeds by Agilent oilseed microarrays at the whole-genome level between 180 kg ha–1 nitrogen fertilizer treatment and CK showed that Zhongshuang 11 contained 827 differential expressed genes, Parter comprised 3 676 differentially expressed genes, and two varieties jointly shared 278 differentially expressed genes, including 151 genes up-regulated and 80 genes down-regulated, and four genes with at least 10-fold difference expression. The functional classification of differentially expressed genes indicated that they mainly had binding, catalytic activities and transcriptional regulation activities. Most of them were involved in cellular, metabolic, and stimulative processes. The genes (about 50%) without function annotation should be studied further. Additionally, the expression of eight differentially expressed genes was validated by Quantitative RT-PCR. The results from e two methods were 94% consistent, which indicated that the microarray results were biologically reproducible. Our results shade light on exploring mechanism of nitrogen response and nitrogen-tolerance genotype identification in B. napus.

      TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY
      Differences of Nitrogen Absorption and Utilization in Rice Varieties with Different Productivity Levels
      HUO Zhong-Yang,GU Hai-Yong,MA Qun,YANG Xiong,LI Min,LI Guo-Ye,DAI Qi-Gen,XU Ke,WEI Hai-Yan,GAO Hui,LU Yan,ZHANG Hong-Cheng
      Acta Agron Sin. 2012, 38(11):  2061-2068.  doi:10.3724/SP.J.1006.2012.02061
      Abstract ( 1550 )   PDF (295KB) ( 1213 )   Save
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      The field experiment was carried out using 50 early-maturing late Japonica rice varieties, adopted in the region of Yangtze River, with seven nitrogen application levels (0, 150.0, 187.5, 225.0, 262.5, 300.0, 337.5 kg ha-1) to investigate the differences of the nitrogen absorption and utilization. We defined the highest rice yields under different nitrogen levels as the highest population productivity of N fertilization (HPPNF). The main results indicated that the top- and high-level yielding varieties had extremely significant larger amount of population spikelets than the middle- and low-level yielding varieties, and this superiority of total spikelets was mainly due to the extremely remarkable increase of spikelets per panicle. The nitrogen content and nitrogen absorption rate in plant at jointing, heading and maturity were all significantly increased with increasing productivity level. In transplanting to jointing and jointing to heading stages, there were no significant differences in ratio of nitrogen accumulation among four productivity levels, but a trend of decrease with the increase of productivity level. In heading to maturity stages, the ratio of nitrogen accumulation was significantly increased with increasing productivity level, which was 14.94% for top-level yielding cultivars. With the productivity level increasing, the apparent nitrogen recovery efficiency, agronomic nitrogen use efficiency and physiological nitrogen use efficiency were significantly increased. Taking into consideration of the production capacity and the apparent nitrogen recovery efficiency of all 50 rice varieties, 13 varieties with high yield and high nitrogen efficiency were selected and recommended.

      Response of Nitrogen Use Efficiency to Plant Density and Nitrogen Application Rate for Maize Hybrids from Different Eras in Heilongjiang Province
      QIAN Chun-Rong,YU Yang,GONG Xiu-Jie,JIANG Yu-Bo,ZHAO Yang,HAO Yu-Bo,LI Liang,ZHANG Wei-Jian
      Acta Agron Sin. 2012, 38(11):  2069-2077.  doi:10.3724/SP.J.1006.2012.02069
      Abstract ( 1710 )   PDF (299KB) ( 1192 )   Save
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      n this study, eight typical maize hybrids released from 1970s to 2000s in Heilongjiang Province were selected to investigate the responses of grain yield, Nitrogen (N) partial factor productivity (PFP), N agronomic efficiency (NAE) and N recovery efficiency (NRE) to N application rate and plant density in 2009 and 2010. During the variety improvement period of 1970s–2000s, PFP and NRE increased significantly by 3.41 kg kg-1 10 yr-1 and 2.26 per cent 10 yr-1, respectively. NAE increased significantly from 1970 to 2000 and descended after 2000. N harvest index decreased significantly by 1.51 per cent 10 yr-1. Grain N, stem N and leaf N accumulations rose significantly by 0.09, 0.07, and 0.12 g plant-1 10 yr-1. There were significant interactions among eras, plant densities and N application rates for above indicators. Nitrogen use efficiency exhibited decline trends with increasing N application rate and parabolic shape response to increasing plant density, and got the maximum in the population range of 50 000–70 000 plants ha-1. The theoretical optimal plant density for the maximal NUE was higher in newer hybrids than in the older ones. Higher plant population led to decrease N accumulation and N harvest index in grain, stem and leaf for all hybrids from different eras. Higher N application rate tended to promote N accumulation in grain, stem and leaf, but had different effects on N harvest index for hybrids from different eras. Compared to the varieties released abroad, there is a great potential in maize variety improvement for high-yield and higher-NUE in Northeast China. According to the existing variety’s traits, increasing plant density can enhance not only maize yield but also NUE.

      Characteristics and Evaluation Parameters Associated with Cooking Quality of Chinese Fresh Noodle
      ZHANG Yan,YAN Jun,XIAO Yong-Gui,WANG De-Sen,HE Zhong-Hu
      Acta Agron Sin. 2012, 38(11):  2078-2085.  doi:10.3724/SP.J.1006.2012.02078
      Abstract ( 1997 )   PDF (236KB) ( 1402 )   Save
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      Noodle cooking quality plays an important role in assessing processing quality of Chinese fresh noodle. Forty-six Chinese wheat cultivars and advanced lines from the Northern Plain and the Huang-Huai River Valleys Winter Wheat Regions were used to determine the relationship between wheat quality characters and evaluation parameters of Chinese fresh noodle cooking quality including total organic matter (TOM), cooking losses, water sorption, and noodle stickiness. The results indicated that large variations were observed in milling quality, dough rheology characteristics, starch properties, and noodle cooking quality parameters including TOM value, cooking losses, and noodle stickiness. Extensogram energy and maximum resistance contributed negatively to TOM value, with correlation coefficients of -0.66 (P<0.01) and -0.56 (P<0.01), respectively. Correlation coefficients between Farinogram stability, Extensogram energy and maximum resistance and cooked noodle weights with optimal cooking (6 min) and overcooking (10 min) ranged from -0.55 to -0.63 (P<0.01). Farinogram mixing tolerance index was significantly and positively correlated with cooked noodle weights for 6min and 10min, with correlation coefficients of 0.67 (P<0.01) and 0.69 (P<0.01), respectively. Starch pasting temperature was significantly and positively correlated with cooked noodle weights for 10 min (r = 0.60, P<0.01). This suggested that increased flour protein content and dough gluten strength contributed positively to noodle cooking quality, flour protein property was the major factor in determining noodle cooking quality, and noodle cooking quality was also affected slightly by starch pasting parameters. TOM value was significantly and positively correlated with cooked noodle weights for 6 min and 10 min, with correlation coefficients of 0.66 (P<0.01) and 0.69 (P<0.01), respectively. Correlation coefficient between cooked noodle weight with 6 min and 10 min cooking time was 0.86 (P<0.01). Therefore, it was recommended that cooked noodle weight for 10 min could be an important parameter for evaluation of noodle cooking quality. The cooked noodle weight for 10 min (10 g fresh noodle) should be no more than 21.0 g forgood noodle cooking quality in Chinese wheat samples.

      Effect of Nitrogen Fertilizer Application on Eating Quality of Early-Maturing Late Japonica Rice in Jiangsu and Its Difference among Varieties
      CHEN Ying-Ying,HU Xing-Xing,CHEN Jing-Du,YANG Xiong,MA Qun,CHEN Qiao,GE Meng-Jie,DAI Qi-GEN
      Acta Agron Sin. 2012, 38(11):  2086-2092.  doi:10.3724/SP.J.1006.2012.02086
      Abstract ( 1616 )   PDF (206KB) ( 1287 )   Save
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      A field experiment was carried out with 50 early-maturing late japonica. The main results showed that with increasing nitrogen fertilizer, the eating quality was decreased. There were positive correlations between taste value and gel consistency, PKVand BDV, but negative correlations between taste value and amylose content, protein content, HTV, FNV, SBV, and PT.Eighteen varieties better than others were screened with the taste value between 53.9 and 64.0. The application of nitrogen hadthe different effects on indexes of eating qualities among varieties.According to responses of taste value to nitrogen application levels, these varietieswere divided into three groups (insensitive, intermediate, and sensitive). The insensitive group with high taste value containe varieties, such as Tongeng 981, Nangeng 46, Zhendao 158, and Changyou 2, which taste values were relatively stable under all nitrogen levels, even under high nitrogen levels. Another group with high taste value was sensitive to nitrogen application levels, such as Zhendao 661 and M1148, which we should pay attention to the effect ofN fertilizerapplication to taste value in cultivating. The rest was intermediate. In terms of their different responses of taste value to nitrogen application levels, good cooking quality could be obtained through properly reducing nitrogen application. rice varieties released in late decade in Jiangsu Province, to study the effects of seven nitrogen application levels (0, 150, 187, 225, 262, 298, and 337 kg ha-1) on the difference in eating qualities.

      Effects of Potassium Application Rate on Stem Lodging Resistance of Summer Maize under High Yield Conditions
      LI Bo,ZHANG Ji-Wang,CUI Hai-Yan,JIN Li-Bin,DONG Shu-Ting,LIU Peng,ZHAO Bin
      Acta Agron Sin. 2012, 38(11):  2093-2099.  doi:10.3724/SP.J.1006.2012.02093
      Abstract ( 1806 )   PDF (415KB) ( 1462 )   Save
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      Lodging is an important factor to limit grain yield of summer maize. This study is to investigate the effects of potassium (K) application rate on the lodging resistance of summer maize under high yield cultivation conditions to that providing a reference of reasonable cultivation way. Two cultivars of summer maize (Zea mays L.), Denghai 661 (DH661) and Zhengdan 958 (ZD958), were used and six levels of K application rates were designed. The results showed that the grain yield and bleeding sap were increased at the first, and then gradually decreased with the increment of K application rate. Applied K could enhance rind penetrometer resistance by 17.9%, 25.57% in maximum for DH661 and ZD958. But the increase in different internodes was different. The increased vascular bundles especially the small vascular bundles, per unit area of crease rection of internode, were the key reason to improve stalk puncture strength. DH661 and ZD958 reached the highest yield at the K application rate of 180 kg ha-1 and 240 kg ha-1 respectively.

      Effects of Nitrogen Management in Wheat Season on Matter Production and Nitrogen Use Efficiency in Winter Wheat–Summer Maize Rotation System
      WU Guang-Lei,ZENG Yan,GUO Li-Yue,CUI Zheng-Yong,LI Yong,YIN Yan-Ping,WANG Zhen-Lin,JIANG Gao-Ming
      Acta Agron Sin. 2012, 38(11):  2100-2107.  doi:10.3724/SP.J.1006.2012.02100
      Abstract ( 1939 )   PDF (268KB) ( 1119 )   Save
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      Management of nitrogen (N) fertilizer is a constant topic in crop cultivation aiming at high-yield and good-quality production as well as environmental protection. In winter wheat–summer maize rotation system, N fertilizer input in the wheat season has a subsequent influence on the maize season. In this study, we carried out a two-year field experiment in Linyi of Shandong Province, China from 2009 to 2011 with treatments of four N application rates and two topdressing schemes. In the wheat season, the N application rates were 0 (N0), 168.75 (N1), 225 (N2), and 281.25 kg ha-1 (N3), and the topdressing N was given at jointing stage (S1) and jointing + anthesis stage (S2). In the maize season, N fertilizer of 60 kg ha-1 was applied before sowing and 90 kg ha-1at 12-leaf stagein all treatments except for N0 (no N applied in the maize season as well). The dry matter accumulation and distribution, grain yield, nitrogen agronomic use efficiency (NAUE), and nitrogen partial factor productivity (NPFP) were investigated in both wheat and maize seasons. Under the S1 condition, dry matter accumulations in grains of wheat and maize and the biomasses of maize as well as the biomass of both crops heightened with N rate increased, but the grain yields of wheat and maize had no significant differences between N2 and N3 treatments. As the N rate increased, NAUE and NPFP decreased in winter wheat but increased gradually in summer maize; however, the difference in NPFP between N2 and N3 was not significant. Under the S2 condition, the dry matter accumulations in grain, biomasses, and grain yields of wheat and maize had no significant increases in N3 treatment compared to N2 treatment, although the N application amount was promoted by 25% in N3 treatment. The NAUE and NPFP of wheat decreased significantly due to the more distribution of dry matter in vegetative organs of wheat in N3 treatment than in N2 treatment; whereas, the NAUE and NPFP of maize had no significant changes. In the wheat season, compared to S1, S2 was favorable to increase dry matter accumulationsin grain and vegetative organs, biomass, grain yield, NAUE, and NPFP of winter wheat in treatments N1 and N2, but had no significant effects in treatment N3; in the maize season, S2 showed positive effects on these indices of summer maize in treatments N1, N2, and N3. With the comprehensive consideration of total biomass and grain yield of wheat and maize, NAUE, and NPFP, total N fertilizer of 225 kg ha-1 in wheat season associated with topdressing at jointing and anthesis stages of wheatare recommended as the optimal management mode in winter wheat–summer maize rotation system under similar conditions to this experiment.

      Evaluation of Drought Resistance in Rapeseed (Brassica napus L.) DH Lines at Seedling Stage
      LI Zhen,MEI Shu-Fang,MEI Zhong,LIU Fang,ZHOU Guang-Sheng,WU Jiang-Sheng
      Acta Agron Sin. 2012, 38(11):  2108-2114.  doi:10.3724/SP.J.1006.2012.02108
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      Drought stress in autumn, winter and spring has endangered the production and quality of rapeseed in China.The objective of the study was to evaluate drought resistance of the rapeseed (Brassica napus L.) DH (doubled haploid) population and screen the most drought-resistant DH lines. For phenotyping, the rapeseed lines from DH population [(Huyou 15×5900) F4 × (No2127-17×275B) F4] were grown in plastic pots. Six traits including plant height, root length, shoot dry weight (SDW), root dry weight (RDW), root/shoot ratio and total dry weight (TDW) together with DRC (drought resistance coefficient) of all the traits were used to evaluate the drought resistance of the 118 DH lines and their parents. The results showed that the six responsive traits changed significantly under the drought conditions compared with the control in the experiment and the effects of drought were much more severe on RDW than on other traits. All traits showed transgressive and continuous distribution under both drought and control conditions. The correlation among all the traits investigated in the study indicated that DRC of SDW, RDW and TDW could be used as available evaluation indices for drought resistance. Lines 032, 034, and 035 screened from the population were the most drought-resistant lines and 006, 091, and 104 were the most drought-sensitive lines.

      Changes in Microbial Flora and Bacterial Physiological Group Diversity on Leaf Surface of Transgenic Bt Corn
      TIAN Xiao-Yan,ZHAO Lei,ZHAO Hui,SUN Hong-Wei,LI Fan,YAN Shi-Lei,LU Xing-Bo
      Acta Agron Sin. 2012, 38(11):  2115-2121.  doi:10.3724/SP.J.1006.2012.02115
      Abstract ( 1664 )   PDF (258KB) ( 1005 )   Save
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      In order to evaluate the effect of transgenic maize on epiphytes, the transgenic Bt maize Mon810 and its parental non-transgenic maize were grown in field to study the quantitative changes of culturable microorganisms and the diversity of bacterial functional groups at different growth stages in 2010 and 2011. Although there were differences in the colony-forming unites of culturable bacteria, actinomycetes and fungi of phyllosphere between Bt maize and non-Bt maize in different growing periods and different years, the trend of annual differences was basically consistent at the same growth period:the quantity of microbes in seeding stage is fewest and reaches the peak in the late of growing period. Compared with the controls, transgenic Bt maize seemed to stimulate the reproduction of phyllosphere fungi. However, no significant effect on the populations of actinomycete was observed. Significant differences were detected for bacteria at jointing stage, trumpet stage, silking stage and milking stage. In 2010, the Shannon-Wiener index, Simpson index and evenness index of bacteria physiological groups were higher at seeding, jointing and full-ripe stage, but lower at trumpet stage, tasseling stage, silking stage and milk stage. In 2011, the population characteristic parameters of the microorganisms of Bt-corn are more than the ones of non-Bt corn in the whole growing period except trumpet stage. It may be concluded there was some difference in terms of impacts between transgenic maize and non-transgenic maize, while the main impacts on microbial community composition were likely due to different years and different growth periods.

      RESEARCH NOTES
      Differential Expression of Chloroplast Genes in Chlorophyll-Deficient Wheat Mutant Mt135 Derived from Space Mutagenesis
      XIA Jia-Ping,GUO Hui-Jun,XIE Yong-Dun,ZHAO Lin-Shu,GU Jia-Yu,ZHAO Shi-Rong,LI Jun-Hui,LIU Lu-Xiang
      Acta Agron Sin. 2012, 38(11):  2122-2130.  doi:10.3724/SP.J.1006.2012.02131
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      The leaf color ofchlorophyll-deficient mutant Mt135 (Triticum aestivum L.) shows albino, striped, and green phenotypes. The number and structure of chloroplasts in the albino plant and albino tissue of striped plants are significantly different from those in the wild type, which results in the complete loss of photosynthetic function in albino plant/tissue. The expression alteration of chloroplast genes in young seedlings of albino Mt135 and wild type was studied using RT-PCR technique, and 40 genes were identified to be differentially expressed in the albino plant/tissue, including 18 genes up-regulated and 22 genes down-regulated. These genes categorized into four groups encoded proteins responding photoreaction (31%), enzymes of energy metabolism in chloroplast (27%), ribosome biogenesis (25%), and tRNA biosynthesis (17%). The alteration trends of 13 up-regulated genes detected in the striped plants were similar to those in albino seedlings. Among these differentially expressed genes, gene families of psb, psa, and ycf encoding structural proteins of photosystem II and I were down-regulated significantly, genes encoding ribosomal protein subunits were altered, especially expressions of gene rps14 encoding small ribosomel subunit protein and 23S rDNA encoding 23S rRNA were down-regulated significantly. It was concluded that the differential expression of these genes related with proteins responding photoreaction, enzymes of energy metabolism in chloroplast, ribosome biogenesis and tRNA biosynthesis may induce the phenotype of mutant Mt135.

      Solvent Retention Capacities of Varieties from Different Wheat Regions in China
      ZHANG Yong,JIN Yan,ZHANG Bo-Qiao,ZHANG Xiao,XU Liang,XU Yang,CHENG Shun-He,XU Chen-Wu
      Acta Agron Sin. 2012, 38(11):  2131-2137.  doi:10.3724/SP.J.1006.2012.02131
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      Solvent retention capacity (SRC) is an important index for predicting flour functionality in soft wheat, which includes water SRC, 50% lactic acid SRC, 5% sodium carbonate SRC, and 50% sucrose SRC. To understand the SRCcharacter of wheat varieties in China, We measured four types of SRC in 181 varieties from different wheat regions in 2008–2009 and 2009–2010 growing seasons. According to analysis of variance, all types of SRC were significantly influenced by genotype, year, and wheat region (P < 0.01 or 0.05). Besides, the interactions of genotype ´ year and year ´ wheat region were also significant (P < 0.01). The principal component analysis showed that component 1 had initial eigenvalue higher than 1.0, and explained 72.3% of total SRC variance. The result of clustering based on principal score showed that there was obvious variation of SRC among the 181 varieties, and each wheat region contained varieties with different SRC characteristics. The Middle and Low Yangtze Valleys Autumn-Sown Spring Wheat Zone had more genetic resources with low SRC than other wheat regions, and varieties with medium and high SRC were mostly distributed in the Yellow and Huai River Valleys Facultative Wheat Zone. A few varieties with low SRC were selected, including Abbondanza, Fazhang 5, Huaimai 17, Ningmai 3, Ningmai 6, Wanmai 48, Xuan 7, Yangmai 13, Yangmai 17, and Zhengmai 004, which can be used in breeding and quality improvement of weak-strength wheat.

Co-sponsored:
the Crop Science Society of China
the Institute of Crop Science, CAAS
China Science Publishing & Media Ltd.
Published: Science Press
Editor-in-chief: Wan Jian-min
Associate Editors-in-Chief:
Chen Xiao-ya Yang Jian-chang Zhang Xian-long Wang Jian-kang Xu Ming-liang Liu Chun-ming Wang Dao-wen Sun Chuan-qing Ding Yan-feng Jin Wei-wei Chu Cheng-cai Cheng Wei-hong
Director of the editorial department:
Yan Chun-ling
CN 11-1809/S
ISSN 0496-3490
Post subscription code: 82-336

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  • Started in 2013
  • Covered by SCIE
  • Open access in ScienceDirect

Editor in chief: Wan Jian-min
CN 10-1112/S
ISSN 2095-5421, 2214-5141(online)
Online published:
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E-mail: cropjournal@caas.cn
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