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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (11): 1988-1996.doi: 10.3724/SP.J.1006.2012.01988

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Preliminary Functional Analysis of a Rice Leaf Senescence Up-Regulated Gene

SUN Bo,ZHOU Yong,LIN Yong-Jun*   

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2012-04-10 Revised:2012-07-05 Online:2012-11-12 Published:2012-09-10
  • Contact: 林拥军, E-mail: yongjunlin@mail.hzau.edu.cn, Tel: 027-87281719

Abstract:

Leaf senescence is the last stage in leaf development. It has important theoretical and practical significance to study the mechanism on rice leaf senescence and the find out a way to purposefully control or delay leaf senececne. In this study, we identified a rice leaf senescence up-regulated gene A12 (LOC_Os 12g33120) according to the leaf senescence gene expression microarray data. The analysis from the rice gene expression profile database in whole growth period and qRT-PCR result of A12 expression in flag leaves at different stages after heading further confirmed that A12 was a leaf senescence up-regulated gene. Bioinformatic analysis found many phytohormone-responsive cis-acting elements in A12 promoter. Quantitative real-time PCR analysis indicated the A12 gene were up-regulated by JA and KT treatments, but not apparently induced by BR, GA, IAA, and ABA. Research on the rice T-DNA insertion mutant of A12 found that loss of function of this gene led to premature senescence of flag leaf. These results lay a foundation for the further functional analysis of this gene.

Key words: Rice, Leaf senescence-associated gene, Function analysis

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