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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (11): 1969-1976.doi: 10.3724/SP.J.1006.2012.01969

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Resistance to Heterodera filipjevi in Triticum aestivumDasypyrum villosum Germplasm

ZHANG Jia-Jia1,YUAN Hong-Xia1,ZHANG Rui-Qi2,XING Xiao-Ping1,DAI Jun-Li1,NIU Ji-Shan1,LI Hong-Lian1,*,CHEN Pei-Du2,*   

  1. 1 College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China; 2 Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2012-04-05 Revised:2012-07-05 Online:2012-11-12 Published:2012-09-10
  • Contact: 李洪连, E-mail: honglianli@sina.com; 陈佩度, E-mail: pdchen@njau.edu.cn

Abstract:

Heterodera filipjevi is a new pathogenic species of cereal cyst nematode (CCN) in China, which has a potential threat to wheat production security. Wheat relatives are important resources of multiple resistance genes to many biotic and abiotic stresses, including CNN resistance genes. Using relative resistance index (RRI) as the evaluation indicator, we screened resistant resources against H. filipjevi from 20 wheat relatives after artificial inoculation, and found Dasypyrum villosum highly and stably resistant to H. filipjevi Xuchang population. Three sets of common wheat (Triticum aestivum L. cv. Chinese Spring) chromosome addition lines with individual D. villosum chromosome 1V to 7V, as well as 6VS translocation lines from different origins, were evaluated for CCN resistance. The Triticum aestivumD. villosum 6V chromosome addition line showed high resistance to CCN with RRI higher than 0.90 in all sets of addition lines; however, the translocation lines 6VS were susceptible to CNN with RRI ranging from 0.43 to 0.51. Therefore, the CCN resistance gene is most probably located on chromosome 6VL.

Key words: Wheat relatives, Dasypyrum villosum, Heterodera filipjevi, GISH, Gene location

[1]Rivoal R, Nicol J M. Past research on the cereal cyst nematode complex and future needs. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal Cyst Nematodes: Status: Research and Outlook. Ankara, Turkey: CIMMYT Press, 2009. pp 3−10



[2]Mcdonald A H, Nicol J M, Nematode parasites of cereals. In: Luc M, Sikora R A, Bridge J, eds. Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, 2nd Edn. Wallingford: CABI Publishing, 2005. pp 131−191



[3]Handoo Z A. A key and compendium to species of the Heterodera avenae group (Nematoda: Heteroderidae). J Nematol, 2002, 34: 250–262



[4]Abidou H, El-Ahmed A, Nicol J M, Bolat N, Rivoal R, Yahyaoui A. Occurrence and distribution of species of the Heterodera avenae group in Syria and Turkey. Nematologia Mediterranea, 2005, 33: 195



[5]Rivoal R, Cook R. Nematode pests of cereals. In: Evans K, Trudgill D L, Webster J M, eds. Plant parasitic nematodes in temperate agriculture, Wallingford: CABI Publishing, 1993. pp 259−303



[6]Chen P-S(陈品三), Wang M-Z(王明祖), Peng D-L(彭德良). Preliminary report of identification on cereal cyst nematode of wheat in China. Sci Agric Sin (中国农业科学), 1991, 24(5): 89−91 (in Chinese with English abstract)



[7]Peng D L, Nicol J M, Li H M, Hou S Y, Li H X, Chen S L, Ma P, Li H L, Riley I T. Current knowledge of cereal cyst nematode (Heterodera avenae) on wheat in China. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal Cyst Nematodes: Status, Research and Outlook. Ankara, Turkey: CIMMYT Press, 2009. pp 29−34



[8]Wang X(王暄), Liang Z-W(梁中伟), Pei S-A(裴世安), Le X-H(乐秀虎), Li H-M(李红梅), Peng D-L(彭德良). Sequences analysis of rDNA-ITS region of cereal cyst nematodes on wheat from Jiangsu province. Nanjing Agric Univ (南京农业大学学报), 2010, 33(6): 55−62 (in Chinese with English abstract)



[9]Peng D L, Ye W X, Peng H, Gu X C. First report of the cyst nematode (Heterodera filipjevi) on wheat in Henan Province, China. Plant Dis, 2010, 94: 1262



[10]Li H L, Yuan H X, Sun J W, Fu B, Nian G L, Hou X S, Xing X P, Sun B J. First record of the cereal cyst nematode Heterodera filipjevi in China. Plant Dis, 2010, 94: 1505



[11]Fu B, Yuan H X, Zhang Y, Hou X S, Nian G L, Zhang P, Xing X P, Sun B J, Riley I T, Li H L. Molecular characterisation of cereal cyst nematodes in winter wheat on the Huang-Huai floodplain of China using RFLP and rDNA-ITS sequence analyses. Australas Plant Pathol, 2011, 40: 277−285



[12]Nian G-L(年高磊), Sun J-W(孙君伟), Hou X-S(侯兴松), Fu B(付博), Yuan H-X(袁虹霞), Xing X-P(邢小萍), Li H-L(李洪连). Identification of pathotypes of three populations of Heterodera filipjevi in Henan province. In: Liao J-L(廖金铃), Peng D-L(彭德良), Duan Y-X(段玉玺), eds. Nematology Research in China (中国线虫学研究), Vol. 3. Beijing: China Agricultural Science and Technology Press, 2010. pp 130–133 (in Chinese)



[13]Gao X(高秀), Cui L(崔磊), Li H-L(李洪连), Wang X-M(王晓鸣), Tang W-H(唐文华), Conner R L, Lin X-H(林小虎), Li H-J(李洪杰). Resistance of Triticum durum Cultivars Waskana and Waskowa to Cereal Cyst Nematode, Heterodera filipjevi and H. avenae. Acta Agron Sin (作物学报), 2012, 38(4): 571–577 (in Chinese with English abstract)



[14]Zhang W-M(张万民), Duan Y-X(段玉玺), Chen L-J(陈立杰), Liang C(梁晨). Effect of agrochemicals and biocontrol productions on soil nematode community dynamics. Chin J Appl Ecol(应用生态学报), 2002, 13(5): 638–640 (in Chinese with English abstract)



[15]Zheng J-W(郑经武). Cereal cyst nematode and its control in Australia. World Agric (世界农业), 1995, (3): 36–37 (in Chinese)



[16]Yuan H-X(袁虹霞), Zhang F-X(张福霞), Zhang J-J(张佳佳), Hou X-S(侯兴松), Li H-J(李洪杰), Li H-L(李洪连). Resistance of CIMMYT wheat germplasm to Heterodera filipjevi Xuchang population from Henan Province, China. Acta Agron Sin (作物学报), 2011, 37(11): 1956–1966 (in Chinese with English abstract)



[17]Wang Z-Y(王振跃), Gao S-F(高书峰), Li H-L(李洪连), Sha G-L(沙广乐), Yu M-Q(余懋群). Resistance of different wheat cultivars to cereal cyst nematode. J Henan Agric Sci (河南农业科学), 2006, (5): 50–52 (in Chinese)



[18]Jahier J, Abelard P, Tanguy M, Dedryver F, Rivoal R, Khatkar S, Bariana H S, Koebner R. The Aegilops ventricosa segment on chromosome 2AS of the wheat cultivar ‘VPM1’ carries the cereal cyst nematode resistance gene Cre5. Plant Breed, 2001, 120: 125–128



[19]Eastwood R F, Lagudah E S, Appels R. A directed search for DNA sequences tightly linked to cereal cyst nematode resistance genes in Triticum tauschii. Genome, 1994, 37: 311–319



[20]Barloy D, Lemoine J, Abelard P, Tanguy A M, Rivoal R, Jahier J. Marker-assisted pyramiding of two cereal cyst nematode resistance genes from Aegilops variabilis in wheat. Mol Breed, 2007, 20: 31–40



[21]Yu M Q, Person D F, Jahier J. Resistance to the root knot nematode, Meloidogyne naasi transferred from Aegilops variabilis to breed wheat. Agronome, 1990, 6: 451–456



[22]Yu M Q, Jahier J, Person D F. Chromosomal location of a gene (Rkn-mnl) for resistance to the root-knot nematode transferred into wheat from Aegilops variabilis. Plant Breed, 1995, 114: 358–360



[23]Eastwood R F, Lagudah E S, Appels R, Hannah M, Kollmorgen J F. Triticum tauschii: a novel source of resistance to cereal cyst nematode (Heterodera avenae). Australas J Agric Res, 1991, 42: 69–77



[24]Jing J-X(井金学), Fu J(傅杰), Yuan H-X(袁红旭), Wang M-N(王美南), Shang H-S(商鸿生), Li Z-Q(李振岐). A preliminary study on heredity of the resistance to stripe rust in three wild relatives of wheat. Acta Phytopathol Sin (植物病理学报), 1999, 29(2): 147–150 (In Chinese with English abstract)



[25]Liu P(刘萍), Yang B-J(杨宝军), Chen P-D(陈佩度). Transfer of disease resistance of Triticum aestivum-Haynaldia villosa 6VS/6AL translocation lines in different wheat background. J Nanjing Agric Univ (南京农业大学学报), 2004, 27(2): 1–5 (in Chinese with English abstract)



[26]Blanco A, Resta P, Simeone R, Parmar S, Shewry P R, Simeone R, Sabelli P, Lafiandra D Chromosomal location of seed storage protein genes in the genome of Dasypyrum villosum (L.) Candargy. Theor Appl Genet, 1991, 82: 358–362



[27]Blanco A, Simeone R, Tanzarella O A, Greco B. Morphology and chromosome pairing of a hybrid between Triticum durum Desf. and Haynaldia villosa (L.) Schur. Theor Appl Genet, 1983, 64: 333–337



[28]Qi L L, Chen P D, Liu D J, Zhou B, Zhang S Z. Development of translocation lines of Triticum aestivum with powdery mildew resistance introduced from Haynaldia villosa. Li Z S, Xin Z Y. Proceedings of the 8th international wheat genetics symposium. Beijing: Chinese Agricultural Scientech Press, 1993. pp 333–337



[29]Chen P D, Qi L L, Zhou B, Zhang S Z, Liu D J. Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet, 1995, 91: 1125–1128



[30]Qi L L, Pumphrey M O, Friebe B, Chen P D, Gill B S. Molecular cytogenetic characterization of alien introgressions with gene Fhb3 for resistance to Fusarium head blight disease of wheat. Theor Appl Genet, 2008, 117: 1155–1166



[31]Jiang J, Gill B S. Sequential chromosome banding and in situ hybridization analysis. Genome, 1993, 36: 792–795



[32]Zhang P, Li W, Friebe B, Gill B S. Simultaneous painting of three genomes in hexploid wheat by BAC-FISH. Genome, 2004, 47:979−987



[33]Li H J, Conner R L, Chen Q, Jia X, Li H, Graf R J, Laroche A, Kuzyk A D. Different reactions to the wheat curl mite and Wheat streak mosaic virus in various wheat-Haynaldia villosa 6V and 6VS lines. Plant Dis, 2002, 86: 423–428

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