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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (12): 2107-2114.doi: 10.3724/SP.J.1006.2013.02107

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Molecular Mapping of Powdery Mildew Resistance Gene pmCH83 in a Putative Wheat–Thinopyrum intermedium Cryptic Introgression Line

SUN Cui-Hua1,HOU Li-Yuan1,GUO Hui-Juan2,ZHANG Xiao-Jun2,*,JIA Ju-Qing3,LI Xin2,ZHAN Hai-Xian2,CHANG Zhi-Jian2,*   

  1. 1 Graduate School of Shanxi University, Taiyuan 030006, China; 2 Institute of Crop Science, Shanxi Academy of Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, China; 3 College of Agronomy, Shanxi Agricultural University, Taigu 030801, China
  • Received:2013-05-17 Revised:2013-07-25 Online:2013-12-12 Published:2013-09-29
  • Contact: 畅志坚, E-mail: wrczj@126.com; 张晓军, E-mail: zxjemail@163.com

Abstract:

Wheat introgression line CH09W83 is immune or highly resistant to wheat powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) pathotypes E09, E20, E21, E23, E26, Bg1, and Bg2 at seedling stage in China. This line was derived from the cross between wheat–Thinopyrum intermedium line TAI7047 (resistant) and wheat cultivar Jintai 170 (susceptible). To identify the Bgt resistance gene(s) in CH09W83, we developed segregating populations (F2, F2:3, and BC1) by crossing/backcrossing CH09W83 with susceptible wheat parents. The result of genetic analysis showed that the adult resistance to Bgt E09 in CH09W83 was controlled by a single recessive gene, which was tentatively designated as pmCH83. Using the F2 population of Taichang 29 (susceptible) ´ CH09W83 and 658 pairs of SSR primers, four codominant SSR markers, i.e., Xgwm193, Xgwm251, Xwmc652, and Xgpw7272, were identified to be linked with pmCH83 according to bulked segregant analysis, and the genetic distances between the target gene and flanking markers were 4.3 cM to Xgwm251 and 3.8 cM to Xwmc652. Using Chinese Spring nullitetrasomic and ditelosomic lines, pmCH83 was located on chromosomal arm 4BL of wheat. In combination with evidence of bivalent pairing at metaphase I and GISH, CH09W83 is probably a cryptic wheat–Th. intermedium translocation. Based on pedigree analysis and linkage map, we deduced that pmCH83 is a novel Bgt resistance gene originating from Th. intermedium.

Key words: Cryptic alien introgression, Resistance to powdery mildew, Linkage map, Molecular markers, GISH

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