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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (02): 307-312.doi: 10.3724/SP.J.1006.2017.00307

• RESEARCH NOTES • Previous Articles    

Development of Markers Closely Linked with Wheat Powdery Mildew Resistance Gene Pm48

FU Bi-Sheng1,**,LIU Ying1,2,**,ZHANG Qiao-Feng1,WU Xiao-You1,GAO Hai-Dong3,CAI Shi-Bin1,DAI Ting-Bo2,*,WU Ji-Zhong1,*   

  1. 1 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Jiangsu Provincial Platform for Conservation and? Utilization of Agricultural Germplasm, Nanjing 210014, China; 2 College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; 3 Genepioneer Biotechnologies Co. Ltd., Nanjing 210014, China
  • Received:2016-07-26 Revised:2016-11-02 Online:2017-02-12 Published:2016-11-15
  • Contact: 吴纪中, E-mail: wujz@jaas.ac.cn, Tel: 025-84391667; 戴廷波, E-mail: tingbod@njau.edu.cn, Tel: 025-84395033 E-mail:fbs1006@126.com
  • Supported by:

    This study was supported by the National Key Technology R&D Program of China (2013BAD01B02-12), the China Agriculture Research System (CARS-3-1-17), Jiangsu Provincial Foundation of Agricultural Scienti?c Innovation [CX (14)5006], and the Natural Science Foundation of Jiangsu Province (BK2012783).


Pm48 is a novel powdery mildew resistance gene identified previously in our laboratory. This study aimed at developing close molecular markers for fine mapping of the gene. The ddRAD-sequencing assay revealed 81 SNPs associated with the target gene, in which one converted into the STS marker Xmp931 and three converted into the CAPS markers Xmp928, Xmp930, and Xmp936. We also developed 71 genomic SSR markers according to the genome sequence of Aegilops tauschii. And mapped two of them, Xmp1089 and Xmp1112. Using the 115 F2:3 families derived from the cross of Ningnuomai 1 ´ Tabasco, the target gene was found to be co-segregated with Xmp928 and distal to Xmp1112 with the genetic distance of 3.1 cM towards centromere. In the 671 homozygous susceptible families, Xmp928 also showed co-segregated with the target gene. We also physically mapped Pm48 to the bin of 5DS 0.63–0.67 by using three Chinese Spring 5DS deletion lines.

Key words: Wheat, Powdery mildew resistance gene, Molecular markers, Bulked ddRAD-seq

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